Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,172,507 C→A 100% I445I (ATC→ATA)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,172,5070CA96.7% 562.9 / 7.6 183I445I (ATC→ATA) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (0/6);  new base A (88/89);  total (88/95)
Fisher's exact test for biased strand distribution p-value = 2.94e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 184 total aligned reads displayed.

TGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCA  >  CP009273/4172362‑4172652
                                                                                                                                                 |                                                                                                                                                 
    tgaggTTGGTCGTATGAAGTTCAACCGTTCTCTGATGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAATGCGAAGTCGATGATATAGaccac                                                                                                                                             <  2:2414255/145‑1 (MQ=255)
     gCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGaccacc                                                                                                                                            <  1:1543205/148‑1 (MQ=255)
      cGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCt                                                                                                                                           >  1:3053659/1‑148 (MQ=255)
        gTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCg                                                                                                                                         >  2:3462818/1‑148 (MQ=255)
         ttGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCgg                                                                                                                                        >  1:1135142/1‑148 (MQ=255)
         ttGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCgg                                                                                                                                        >  1:2773296/1‑148 (MQ=255)
          tGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGc                                                                                                                                       >  1:1873527/1‑148 (MQ=255)
            gTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCaa                                                                                                                                     >  2:3016095/1‑148 (MQ=255)
            gTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCaa                                                                                                                                     >  2:3019773/1‑148 (MQ=255)
            gTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCaa                                                                                                                                     >  2:3286047/1‑148 (MQ=255)
             tCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAAc                                                                                                                                    <  2:1377759/148‑1 (MQ=255)
              cGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAAcc                                                                                                                                   <  1:1363123/148‑1 (MQ=255)
                    aaGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTa                                                                                                                             <  2:1306495/148‑1 (MQ=255)
                     aGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAt                                                                                                                            >  2:1967756/1‑148 (MQ=255)
                     aGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAt                                                                                                                            >  2:1967643/1‑148 (MQ=255)
                        tCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAtccg                                                                                                                         <  1:3003299/148‑1 (MQ=255)
                         cAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAtccgt                                                                                                                        >  1:1978611/1‑148 (MQ=255)
                          aaCCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAtccgtt                                                                                                                       <  1:3275457/148‑1 (MQ=255)
                          aaCCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAtccgtt                                                                                                                       <  1:2246583/148‑1 (MQ=255)
                           aCCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAtccgttc                                                                                                                      <  1:2085497/148‑1 (MQ=255)
                           aCCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAtccgttc                                                                                                                      >  1:1097785/1‑148 (MQ=255)
                           aCCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAtccgttc                                                                                                                      <  1:2085563/148‑1 (MQ=255)
                             cGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAtccgttccg                                                                                                                    <  2:3168057/148‑1 (MQ=255)
                               ttCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATccgttccgtt                                                                                                                  <  2:1302196/148‑1 (MQ=255)
                                tctcTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTg                                                                                                                 >  1:930907/1‑148 (MQ=255)
                                 ctctGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTgg                                                                                                                <  1:3125893/148‑1 (MQ=255)
                                     gctgcGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGaa                                                                                                            <  1:2831201/148‑1 (MQ=255)
                                         cgcgAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATgg                                                                                                        <  1:3161258/148‑1 (MQ=255)
                                                aaaTCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGaaaa                                                                                                 <  1:1893269/148‑1 (MQ=255)
                                                  aTCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAAcc                                                                                               >  2:59201/1‑148 (MQ=255)
                                                      aaGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGtt                                                                                           >  1:616298/1‑148 (MQ=255)
                                                      aaGGGTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGtt                                                                                           <  2:2020461/148‑1 (MQ=255)
                                                       aGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATCTCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTGTCCGTTCCGTTGGCGAAATGGCGGCACTCCAGTTc                                                                                          >  1:2091148/1‑148 (MQ=255)
                                                       aGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTc                                                                                          >  2:2938934/1‑148 (MQ=255)
                                                       aGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGGTGGCGAAATGGCGGAAAACCAGTTc                                                                                          >  2:2300154/1‑148 (MQ=255)
                                                        ggTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTcc                                                                                         >  1:517617/1‑148 (MQ=255)
                                                         gTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCcg                                                                                        <  2:262602/148‑1 (MQ=255)
                                                           tCCGGTCTCCTGAGCAACGCCCACCTCCTAGCTGGTCAGAAACAGCTCATCGATATCCGTAACGGTAAAGGCGAAGCCGATGATATAGACCACCTCGGCAACCGTCGTATCCGGTCCGTTGGCGAAATGGCGGAAAACCAGTTCcgcg                                                                                      <  2:2441590/148‑1 (MQ=255)
                                                           tCCGGTCTCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCcgcg                                                                                      <  1:2257905/148‑1 (MQ=255)
                                                           tCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCcgcg                                                                                      <  2:398248/148‑1 (MQ=255)
                                                                tATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGc                                                                                 <  1:987948/148‑1 (MQ=255)
                                                                tATCCTAAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGc                                                                                 <  1:3324358/148‑1 (MQ=255)
                                                                    cTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTgg                                                                             >  2:2504826/1‑148 (MQ=255)
                                                                        gCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACg                                                                         <  2:2062825/148‑1 (MQ=255)
                                                                            aGACGACATCATTGATGTTATGAAAAAGATCATCGATATAAGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGAATCCGATCCGTTGGCGAAATGGCGGAAAACCAGTTCAGCGTTGGCCTGGTACGTGTa                                                                     >  2:3179010/1‑148 (MQ=255)
                                                                             gacgacATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTag                                                                    >  1:1671565/1‑148 (MQ=255)
                                                                                    tcatTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGc                                                             >  2:1809441/1‑148 (MQ=255)
                                                                                     catTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCg                                                            >  1:3149422/1‑148 (MQ=255)
                                                                                      atTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCgg                                                           >  1:638328/1‑148 (MQ=255)
                                                                                      atTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCgg                                                           <  1:2575629/148‑1 (MQ=255)
                                                                                       ttGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGt                                                          <  1:1446753/148‑1 (MQ=255)
                                                                                           tGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGaaa                                                      <  1:2684867/148‑1 (MQ=255)
                                                                                           tGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCAGTGaaa                                                      >  2:641905/1‑148 (MQ=255)
                                                                                            gTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAag                                                     >  2:1762145/1‑148 (MQ=255)
                                                                                            gTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAag                                                     <  1:2383053/148‑1 (MQ=255)
                                                                                              tATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAagag                                                   >  2:2479808/1‑148 (MQ=255)
                                                                                               aTGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGc                                                  >  2:1304754/1‑148 (MQ=255)
                                                                                               aTGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGc                                                  <  1:1037049/148‑1 (MQ=255)
                                                                                                   aaaaGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgtct                                              <  1:1007258/148‑1 (MQ=255)
                                                                                                   aaaaGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgtct                                              >  1:290410/1‑148 (MQ=255)
                                                                                                   aaaaGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACATCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgtct                                              <  1:784243/148‑1 (MQ=255)
                                                                                                    aaaGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgtctg                                             >  1:1793207/1‑148 (MQ=255)
                                                                                                     aaGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgtctgt                                            >  1:2356580/1‑148 (MQ=255)
                                                                                                     aaGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgtctgt                                            <  1:382433/148‑1 (MQ=255)
                                                                                                      aGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgtctgtc                                           >  1:1846810/1‑148 (MQ=255)
                                                                                                      aGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgtctgtc                                           >  1:1073258/1‑148 (MQ=255)
                                                                                                       gCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgtctgtct                                          <  2:884357/148‑1 (MQ=255)
                                                                                                          cATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTg                                       >  2:859409/1‑148 (MQ=255)
                                                                                                          cATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTg                                       <  1:359381/148‑1 (MQ=255)
                                                                                                           aTCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTgg                                      >  1:3198597/1‑148 (MQ=255)
                                                                                                            tCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTggg                                     >  1:862951/1‑148 (MQ=255)
                                                                                                             cGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGc                                    >  2:2227519/1‑148 (MQ=255)
                                                                                                             cGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGc                                    >  2:1459356/1‑148 (MQ=255)
                                                                                                                 atCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATc                                >  2:2808414/1‑148 (MQ=255)
                                                                                                                 atCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATc                                >  2:2691984/1‑148 (MQ=255)
                                                                                                                 atCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATc                                >  2:2691981/1‑148 (MQ=255)
                                                                                                                 atCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGAGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATc                                >  2:1055729/1‑148 (MQ=255)
                                                                                                                  tCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCt                               >  2:1913581/1‑148 (MQ=255)
                                                                                                                   ccGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTg                              >  2:2045785/1‑148 (MQ=255)
                                                                                                                   ccGTAACGGAAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGGGTAGAGCGAGCGCTGAAAGAGCGTCTGTCTCTGGGCGATCTg                              >  2:2045787/1‑148 (MQ=255)
                                                                                                                    cGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCTGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTgg                             <  2:2403134/148‑1 (MQ=255)
                                                                                                                         cGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATAcc                        <  1:1744976/148‑1 (MQ=255)
                                                                                                                            tAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTg                     >  1:1176513/1‑148 (MQ=255)
                                                                                                                             aaaGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGa                    <  1:59201/148‑1 (MQ=255)
                                                                                                                             aaaGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGa                    >  2:3370526/1‑148 (MQ=255)
                                                                                                                               aGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATg                  >  2:2086132/1‑148 (MQ=255)
                                                                                                                               aGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATg                  <  1:3019773/148‑1 (MQ=255)
                                                                                                                               aGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATg                  <  1:3016095/148‑1 (MQ=255)
                                                                                                                                ggCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGc                 >  2:1346467/1‑148 (MQ=255)
                                                                                                                                    aaGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAACTGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCcaca             <  2:576788/148‑1 (MQ=255)
                                                                                                                                    aaGTCGATGAGATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCcaca             <  2:576759/148‑1 (MQ=255)
                                                                                                                                      gTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAgg           <  2:2390219/148‑1 (MQ=255)
                                                                                                                                      gTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAgg           <  1:1159118/148‑1 (MQ=255)
                                                                                                                                       tCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGa          <  2:1586944/148‑1 (MQ=255)
                                                                                                                                        cGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGat         >  1:1150905/1‑148 (MQ=255)
                                                                                                                                        cGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGat         <  1:2936193/148‑1 (MQ=255)
                                                                                                                                          atgatATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGatat       <  2:869941/148‑1 (MQ=255)
                                                                                                                                            gatATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGa     <  1:2510490/148‑1 (MQ=255)
                                                                                                                                              tataGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATc   >  2:1200017/1‑148 (MQ=255)
                                                                                                                                               atCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCa  <  1:2422043/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
TGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCA  >  CP009273/4172362‑4172652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACG  >  CP009273/4172358‑4172655
                                                                                                                                                     |                                                                                                                                                    
GACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATA                                                                                                                                                      <  SRR19973065.779768/150‑1 (MQ=60)
 ACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAG                                                                                                                                                     <  SRR19973065.1729816/150‑1 (MQ=60)
 ACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAG                                                                                                                                                     <  SRR19973065.3476714/150‑1 (MQ=60)
   TTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGAC                                                                                                                                                   <  SRR19973065.2596663/150‑1 (MQ=60)
   TTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGAC                                                                                                                                                   <  SRR19973065.3107608/150‑1 (MQ=60)
   TTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGAC                                                                                                                                                   <  SRR19973065.760145/150‑1 (MQ=60)
   TTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGAC                                                                                                                                                   <  SRR19973065.2597441/150‑1 (MQ=60)
   TTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGAC                                                                                                                                                   <  SRR19973065.2597402/150‑1 (MQ=60)
    TGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACC                                                                                                                                                  <  SRR19973065.2611675/150‑1 (MQ=60)
     GTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCA                                                                                                                                                 <  SRR19973065.1552859/150‑1 (MQ=60)
        TGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCT                                                                                                                                              >  SRR19973065.3072331/1‑150 (MQ=60)
         GCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTC                                                                                                                                             <  SRR19973065.1553409/150‑1 (MQ=60)
           GGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGG                                                                                                                                           >  SRR19973065.1142500/1‑150 (MQ=60)
           GGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGG                                                                                                                                           >  SRR19973065.2790520/1‑150 (MQ=60)
            GTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGC                                                                                                                                          >  SRR19973065.1885905/1‑150 (MQ=60)
            GTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGC                                                                                                                                          >  SRR19973065.1885864/1‑150 (MQ=60)
                  CGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGT                                                                                                                                    <  SRR19973065.1372143/150‑1 (MQ=60)
                           TTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGT                                                                                                                           >  SRR19973065.1991481/1‑150 (MQ=60)
                            TCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTT                                                                                                                          <  SRR19973065.3021699/150‑1 (MQ=60)
                            TCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTT                                                                                                                          <  SRR19973065.2528230/150‑1 (MQ=60)
                             CAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTC                                                                                                                         >  SRR19973065.1105007/1‑150 (MQ=60)
                              AACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCC                                                                                                                        <  SRR19973065.3295347/150‑1 (MQ=60)
                              AACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCC                                                                                                                        <  SRR19973065.2260839/150‑1 (MQ=60)
                              AACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCC                                                                                                                        <  SRR19973065.2260818/150‑1 (MQ=60)
                               ACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCG                                                                                                                       <  SRR19973065.2098934/150‑1 (MQ=60)
                               ACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCG                                                                                                                       <  SRR19973065.2098868/150‑1 (MQ=60)
                                  GTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTG                                                                                                                    >  SRR19973065.937037/1‑150 (MQ=60)
                                     CTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCG                                                                                                                 <  SRR19973065.3144926/150‑1 (MQ=60)
                                     CTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCG                                                                                                                 <  SRR19973065.1392514/150‑1 (MQ=60)
                                     CTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCG                                                                                                                 <  SRR19973065.2983838/150‑1 (MQ=60)
                                         GCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAAT                                                                                                             <  SRR19973065.2848658/150‑1 (MQ=60)
                                             CGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCG                                                                                                         <  SRR19973065.3180409/150‑1 (MQ=60)
                                             CGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCG                                                                                                         <  SRR19973065.989448/150‑1 (MQ=60)
                                                GAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAA                                                                                                      >  SRR19973065.1144383/1‑150 (MQ=60)
                                                 AAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAA                                                                                                     >  SRR19973065.3024439/1‑150 (MQ=60)
                                                   GAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATACGTTCCGTTGGCGAAATGGCGGAAAAC                                                                                                   >  SRR19973065.3111988/1‑150 (MQ=60)
                                                    AAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACC                                                                                                  <  SRR19973065.1905714/150‑1 (MQ=60)
                                                    AAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACC                                                                                                  >  SRR19973065.1077827/1‑150 (MQ=60)
                                                        CGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTT                                                                                              >  SRR19973065.620251/1‑150 (MQ=60)
                                                        CGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTT                                                                                              >  SRR19973065.2393719/1‑150 (MQ=60)
                                                         GAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATCTCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTGTCCGTTCCGTTGGCGAAATGGCGGCACTCCAGTTC                                                                                             >  SRR19973065.2104577/1‑150 (MQ=60)
                                                         GAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTC                                                                                             >  SRR19973065.2104121/1‑150 (MQ=60)
                                                          AAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCC                                                                                            >  SRR19973065.521037/1‑150 (MQ=60)
                                                               TCCGGTCTCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTT                                                                                       <  SRR19973065.2272193/150‑1 (MQ=60)
                                                               TCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTT                                                                                       <  SRR19973065.2272130/150‑1 (MQ=60)
                                                                    TATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCNT                                                                                  <  SRR19973065.3344645/150‑1 (MQ=60)
                                                                    TATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCT                                                                                  <  SRR19973065.3272690/150‑1 (MQ=60)
                                                                    TATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCT                                                                                  <  SRR19973065.994509/150‑1 (MQ=60)
                                                                    TATCCTAAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCNT                                                                                  <  SRR19973065.3344644/150‑1 (MQ=60)
                                                                     ATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTG                                                                                 >  SRR19973065.508252/1‑150 (MQ=60)
                                                                          GAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACG                                                                            >  SRR19973065.62850/1‑150 (MQ=60)
                                                                          GAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACG                                                                            >  SRR19973065.60596/1‑150 (MQ=60)
                                                                               AAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAG                                                                       >  SRR19973065.1682746/1‑150 (MQ=60)
                                                                                 GACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAG                                                                     <  SRR19973065.109235/150‑1 (MQ=60)
                                                                                   CGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCG                                                                   <  SRR19973065.3183787/150‑1 (MQ=60)
                                                                                       ATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCG                                                               >  SRR19973065.3168539/1‑150 (MQ=60)
                                                                                       ATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCG                                                               >  SRR19973065.1238194/1‑150 (MQ=60)
                                                                                       ATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCG                                                               >  SRR19973065.3168775/1‑150 (MQ=60)
                                                                                        TCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGG                                                              >  SRR19973065.642508/1‑150 (MQ=60)
                                                                                          ATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTG                                                            <  SRR19973065.2591693/150‑1 (MQ=60)
                                                                                           TTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGA                                                           <  SRR19973065.1456105/150‑1 (MQ=60)
                                                                                               TGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGA                                                       <  SRR19973065.2701519/150‑1 (MQ=60)
                                                                                                GTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAG                                                      <  SRR19973065.2397989/150‑1 (MQ=60)
                                                                                                   ATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGT                                                   <  SRR19973065.1043916/150‑1 (MQ=60)
                                                                                                     GAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCT                                                 >  SRR19973065.292301/1‑150 (MQ=60)
                                                                                                      AAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTCCGTGTAGAGCGTGCGGTGAAAGAGCGgcgg                                                >  SRR19973065.1863118/1‑146 (MQ=60)
                                                                                                      AAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTG                                                >  SRR19973065.1804970/1‑150 (MQ=60)
                                                                                                       AAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGT                                               <  SRR19973065.3306032/150‑1 (MQ=60)
                                                                                                       AAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGT                                               <  SRR19973065.1013943/150‑1 (MQ=60)
                                                                                                       AAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGT                                               >  SRR19973065.2371226/1‑150 (MQ=60)
                                                                                                       AAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACATCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGT                                               <  SRR19973065.789450/150‑1 (MQ=60)
                                                                                                       AAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACATCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGT                                               <  SRR19973065.790663/150‑1 (MQ=60)
                                                                                                        AAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTC                                              >  SRR19973065.1080360/1‑150 (MQ=60)
                                                                                                        AAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTC                                              >  SRR19973065.1858978/1‑150 (MQ=60)
                                                                                                        AAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTC                                              >  SRR19973065.1857265/1‑150 (MQ=60)
                                                                                                         AAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCT                                             <  SRR19973065.385107/150‑1 (MQ=60)
                                                                                                           TCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCT                                           <  SRR19973065.1566654/150‑1 (MQ=60)
                                                                                                             TCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGG                                         >  SRR19973065.3217865/1‑150 (MQ=60)
                                                                                                             TCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGG                                         >  SRR19973065.2867112/1‑150 (MQ=60)
                                                                                                              CATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGG                                        >  SRR19973065.868503/1‑150 (MQ=60)
                                                                                                              CATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGG                                        >  SRR19973065.2367594/1‑150 (MQ=60)
                                                                                                              CATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGG                                        <  SRR19973065.2070354/150‑1 (MQ=60)
                                                                                                              CATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGG                                        <  SRR19973065.361907/150‑1 (MQ=60)
                                                                                                                    TATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCT                                  <  SRR19973065.583438/150‑1 (MQ=60)
                                                                                                                    TATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCT                                  >  SRR19973065.754961/1‑150 (MQ=60)
                                                                                                                         GTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATA                             <  SRR19973065.1946867/150‑1 (MQ=60)
                                                                                                                             CGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCT                         >  SRR19973065.1128002/1‑150 (MQ=60)
                                                                                                                             CGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCT                         <  SRR19973065.1756428/150‑1 (MQ=60)
                                                                                                                             CGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCT                         <  SRR19973065.1756486/150‑1 (MQ=60)
                                                                                                                              GGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTG                        >  SRR19973065.1183989/1‑150 (MQ=60)
                                                                                                                                 AAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATG                     <  SRR19973065.59833/150‑1 (MQ=60)
                                                                                                                                   AGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCC                   <  SRR19973065.3038274/150‑1 (MQ=60)
                                                                                                                                   AGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCC                   <  SRR19973065.3034569/150‑1 (MQ=60)
                                                                                                                                   AGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCC                   <  SRR19973065.3037881/150‑1 (MQ=60)
                                                                                                                                         AGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGA             >  SRR19973065.3040393/1‑150 (MQ=60)
                                                                                                                                         AGTCGATGATATAGAACACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGA             >  SRR19973065.2820734/1‑150 (MQ=60)
                                                                                                                                          GTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGAT            >  SRR19973065.1158317/1‑150 (MQ=60)
                                                                                                                                          GTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGAT            <  SRR19973065.1166524/150‑1 (MQ=60)
                                                                                                                                          GTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGAT            <  SRR19973065.1166546/150‑1 (MQ=60)
                                                                                                                                            CGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATAT          <  SRR19973065.2954001/150‑1 (MQ=60)
                                                                                                                                              ATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGA        >  SRR19973065.2782119/1‑150 (MQ=60)
                                                                                                                                                GATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATC      <  SRR19973065.2526264/150‑1 (MQ=60)
                                                                                                                                                   ATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAAC   <  SRR19973065.2437183/150‑1 (MQ=60)
                                                                                                                                                   ATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAAC   <  SRR19973065.2437320/150‑1 (MQ=60)
                                                                                                                                                    TAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATAAACG  <  SRR19973065.3150310/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
GACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACG  >  CP009273/4172358‑4172655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑