Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,172,507 C→A 100% I445I (ATC→ATA)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,172,5070CA98.8% 762.3 / ‑0.9 236I445I (ATC→ATA) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (0/3);  new base A (126/106);  total (126/110)
Fisher's exact test for biased strand distribution p-value = 9.83e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 238 total aligned reads displayed.

TGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATC  >  CP009273/4172362‑4172651
                                                                                                                                                 |                                                                                                                                                
tGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGa                                                                                                                                                <  2:1843626/148‑1 (MQ=255)
 gTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGac                                                                                                                                               <  2:2625201/148‑1 (MQ=255)
   cTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGacca                                                                                                                                             >  1:2103226/1‑148 (MQ=255)
    tGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGaccac                                                                                                                                            <  2:958309/148‑1 (MQ=255)
     gCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGaccacc                                                                                                                                           >  2:1566452/1‑148 (MQ=255)
      cGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCt                                                                                                                                          <  1:3717895/148‑1 (MQ=255)
      cGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCt                                                                                                                                          <  1:2425227/148‑1 (MQ=255)
         ttGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCgg                                                                                                                                       >  1:2452809/1‑148 (MQ=255)
         ttGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCgg                                                                                                                                       >  1:632952/1‑148 (MQ=255)
             tCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAAc                                                                                                                                   >  2:534364/1‑148 (MQ=255)
             tCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAAc                                                                                                                                   >  2:534253/1‑148 (MQ=255)
               gTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACcg                                                                                                                                 <  2:293349/148‑1 (MQ=255)
                tATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACcgt                                                                                                                                >  1:201990/1‑148 (MQ=255)
                 aTGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACcgtc                                                                                                                               >  2:3570182/1‑148 (MQ=255)
                     aGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAt                                                                                                                           <  2:1223379/148‑1 (MQ=255)
                          aaCCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAtccgtt                                                                                                                      <  1:2419298/148‑1 (MQ=255)
                           aCCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAtccgttc                                                                                                                     <  2:1596560/148‑1 (MQ=255)
                             cGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAtccgttccg                                                                                                                   <  1:2520019/148‑1 (MQ=255)
                             cGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAtccgttccg                                                                                                                   <  1:2841884/148‑1 (MQ=255)
                               ttCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATccgttccgtt                                                                                                                 >  1:1190151/1‑148 (MQ=255)
                               ttCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATccgttccgtt                                                                                                                 >  1:3094362/1‑148 (MQ=255)
                               ttCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATccgttccgtt                                                                                                                 >  2:3132876/1‑148 (MQ=255)
                               ttCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATccgttccgtt                                                                                                                 <  2:2221177/148‑1 (MQ=255)
                               ttCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATccgttccgtt                                                                                                                 >  2:3543254/1‑148 (MQ=255)
                               ttCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATccgttccgtt                                                                                                                 >  2:2053217/1‑148 (MQ=255)
                               ttCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATAGACCACCTCGGCAACCGTCGTATcagttccgtt                                                                                                                 >  1:2971414/1‑148 (MQ=255)
                               ttCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCAGTGATGTTATGAAAAAGCTCATCGATATCCGTAACTGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATacgttccgtt                                                                                                                 >  1:711150/1‑148 (MQ=255)
                                    tgctgcGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGa                                                                                                            >  1:3271813/1‑148 (MQ=255)
                                     gctgcGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGaa                                                                                                           <  1:1441857/148‑1 (MQ=255)
                                       tgcGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAAt                                                                                                         <  2:2222767/148‑1 (MQ=255)
                                              agaaATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGaa                                                                                                  >  1:2752431/1‑148 (MQ=255)
                                                aaaTCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGaaaa                                                                                                <  2:3107080/148‑1 (MQ=255)
                                                aaaTCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGaaaa                                                                                                <  1:190806/148‑1 (MQ=255)
                                                 aaTCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAAc                                                                                               >  2:961897/1‑148 (MQ=255)
                                                  aTCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAAcc                                                                                              >  2:3622678/1‑148 (MQ=255)
                                                  aTCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAAcc                                                                                              >  2:331907/1‑148 (MQ=255)
                                                  aTCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAAcc                                                                                              >  2:1107453/1‑148 (MQ=255)
                                                    cGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAg                                                                                            >  2:401897/1‑148 (MQ=255)
                                                     gAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGt                                                                                           <  2:3402967/148‑1 (MQ=255)
                                                      aaGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGtt                                                                                          >  1:3206236/1‑148 (MQ=255)
                                                      aaGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGtt                                                                                          <  1:2533008/148‑1 (MQ=255)
                                                       aGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTc                                                                                         <  2:2103226/148‑1 (MQ=255)
                                                        ggTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGAAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTcc                                                                                        >  2:1284612/1‑148 (MQ=255)
                                                         gTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCcg                                                                                       >  1:3465652/1‑148 (MQ=255)
                                                            ccGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGt                                                                                    <  1:1871187/148‑1 (MQ=255)
                                                                tATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGc                                                                                >  1:3273535/1‑148 (MQ=255)
                                                                 aTCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGcc                                                                               >  2:1772180/1‑148 (MQ=255)
                                                                  tCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCt                                                                              >  1:2985442/1‑148 (MQ=255)
                                                                   ccTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTg                                                                             <  2:2885364/148‑1 (MQ=255)
                                                                   ccTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTg                                                                             >  1:391886/1‑148 (MQ=255)
                                                                         cAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACgt                                                                       <  1:986468/148‑1 (MQ=255)
                                                                         cAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACgt                                                                       >  1:1826003/1‑148 (MQ=255)
                                                                         cAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACgt                                                                       >  1:388355/1‑148 (MQ=255)
                                                                             gacgacATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTag                                                                   <  2:2420752/148‑1 (MQ=255)
                                                                                   atcatTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTg                                                             >  2:1491600/1‑148 (MQ=255)
                                                                                   atcatTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTg                                                             <  1:149815/148‑1 (MQ=255)
                                                                                     catTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCg                                                           >  2:1914384/1‑148 (MQ=255)
                                                                                      atTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGAGAAGTCGATGATATAGTCCAGCTCGGCAAACGTCGTATCCGTTCCGTTGGAGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCgg                                                          <  1:664852/148‑1 (MQ=255)
                                                                                          aTGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGaa                                                      <  1:2073495/148‑1 (MQ=255)
                                                                                          aTGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGaa                                                      >  2:781874/1‑148 (MQ=255)
                                                                                           tGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGaaa                                                     >  2:1653498/1‑148 (MQ=255)
                                                                                              tATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAagag                                                  <  2:3680732/148‑1 (MQ=255)
                                                                                                tGAAAAAGCTCATCGATATCCGTCACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTACGCGTTGGCCTGCAACGTGTAGAGCGTGCGGTGAAAGAGCg                                                >  2:434677/1‑148 (MQ=255)
                                                                                                  aaaaaGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgtc                                              >  1:1566496/1‑148 (MQ=255)
                                                                                                     aaGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgtctgt                                           <  2:2438297/148‑1 (MQ=255)
                                                                                                       gCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgtctgtct                                         >  2:424635/1‑148 (MQ=255)
                                                                                                       gCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgtctgtct                                         >  1:1095194/1‑148 (MQ=255)
                                                                                                         tCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTctct                                       <  1:2254264/148‑1 (MQ=255)
                                                                                                          cATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTg                                      <  2:689177/148‑1 (MQ=255)
                                                                                                          cATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTg                                      >  2:3515024/1‑148 (MQ=255)
                                                                                                            tCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTggg                                    >  2:2526953/1‑148 (MQ=255)
                                                                                                             cGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGc                                   >  1:1310516/1‑148 (MQ=255)
                                                                                                               atatCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGa                                 <  1:402001/148‑1 (MQ=255)
                                                                                                               atatCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGa                                 <  2:2779034/148‑1 (MQ=255)
                                                                                                                tatCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGAt                                <  1:1811986/148‑1 (MQ=255)
                                                                                                                tatCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGAt                                <  2:340195/148‑1 (MQ=255)
                                                                                                                tatCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGAt                                <  1:1125970/148‑1 (MQ=255)
                                                                                                                 atCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATc                               >  2:1696116/1‑148 (MQ=255)
                                                                                                                 atCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATc                               >  2:3588997/1‑148 (MQ=255)
                                                                                                                 atCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATc                               >  2:1697020/1‑148 (MQ=255)
                                                                                                                      tAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGAt                          <  2:2521953/148‑1 (MQ=255)
                                                                                                                        aCGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATAc                        >  2:1960657/1‑148 (MQ=255)
                                                                                                                        aCGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATAc                        >  2:515585/1‑148 (MQ=255)
                                                                                                                            tAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTg                    >  1:2178056/1‑148 (MQ=255)
                                                                                                                            tAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTg                    >  1:1957033/1‑148 (MQ=255)
                                                                                                                            tAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTg                    <  2:1343502/148‑1 (MQ=255)
                                                                                                                             aaaGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGa                   <  2:3717600/148‑1 (MQ=255)
                                                                                                                             aaaGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGa                   <  2:3718008/148‑1 (MQ=255)
                                                                                                                             aaaGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGa                   >  2:2352678/1‑148 (MQ=255)
                                                                                                                                  cGAAGTCGATGATATAGACCACCTCGGAAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCca              <  1:1284612/148‑1 (MQ=255)
                                                                                                                                    aaGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCcaca            <  1:2198480/148‑1 (MQ=255)
                                                                                                                                      gTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAgg          <  1:1773456/148‑1 (MQ=255)
                                                                                                                                       tCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGa         >  1:2135223/1‑148 (MQ=255)
                                                                                                                                         gatgatATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGata       <  2:2747905/148‑1 (MQ=255)
                                                                                                                                         gatgatATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGata       <  2:2749004/148‑1 (MQ=255)
                                                                                                                                             atatAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCCCAGGATATGAt   >  2:768948/1‑148 (MQ=255)
                                                                                                                                             atatAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGAt   <  2:1162516/148‑1 (MQ=255)
                                                                                                                                             atatAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGAt   <  2:3058964/148‑1 (MQ=255)
                                                                                                                                              tataGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATc  >  1:917991/1‑148 (MQ=255)
                                                                                                                                              tataGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATc  >  1:19857/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATC  >  CP009273/4172362‑4172651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAAC  >  CP009273/4172359‑4172654
                                                                                                                                                    |                                                                                                                                                   
ACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAG                                                                                                                                                    <  SRR19973069.253176/150‑1 (MQ=60)
ACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAG                                                                                                                                                    <  SRR19973069.584084/150‑1 (MQ=60)
 CTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGA                                                                                                                                                   >  SRR19973069.2900468/1‑150 (MQ=60)
 CTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGA                                                                                                                                                   <  SRR19973069.419641/150‑1 (MQ=60)
    GTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCA                                                                                                                                                >  SRR19973069.2117745/1‑150 (MQ=60)
       TGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCT                                                                                                                                             <  SRR19973069.3173634/150‑1 (MQ=60)
        GCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTC                                                                                                                                            <  SRR19973069.1905189/150‑1 (MQ=60)
         CGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCG                                                                                                                                           <  SRR19973069.3742169/150‑1 (MQ=60)
         CGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCG                                                                                                                                           <  SRR19973069.2440676/150‑1 (MQ=60)
         CGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCG                                                                                                                                           <  SRR19973069.2441595/150‑1 (MQ=60)
          GGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGG                                                                                                                                          >  SRR19973069.2454731/1‑150 (MQ=60)
          GGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGG                                                                                                                                          >  SRR19973069.2469305/1‑150 (MQ=60)
          GGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGG                                                                                                                                          >  SRR19973069.637126/1‑150 (MQ=60)
          GGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACAACCTCGG                                                                                                                                          >  SRR19973069.2454001/1‑150 (MQ=60)
              GGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACTACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAAC                                                                                                                                      >  SRR19973069.1602544/1‑150 (MQ=60)
                 CGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGT                                                                                                                                   >  SRR19973069.203582/1‑150 (MQ=60)
                 CGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGT                                                                                                                                   >  SRR19973069.203605/1‑150 (MQ=60)
                 CGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGT                                                                                                                                   >  SRR19973069.203626/1‑150 (MQ=60)
                  GTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTC                                                                                                                                  >  SRR19973069.1352792/1‑150 (MQ=60)
                   TATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCG                                                                                                                                 >  SRR19973069.2552414/1‑150 (MQ=60)
                          TTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGT                                                                                                                          <  SRR19973069.18967/150‑1 (MQ=60)
                             AACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCC                                                                                                                       <  SRR19973069.2435641/150‑1 (MQ=60)
                              ACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCG                                                                                                                      >  SRR19973069.3306181/1‑150 (MQ=60)
                                CGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTT                                                                                                                    <  SRR19973069.2536778/150‑1 (MQ=60)
                                CGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTT                                                                                                                    >  SRR19973069.3114756/1‑150 (MQ=60)
                                CGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTT                                                                                                                    <  SRR19973069.2860632/150‑1 (MQ=60)
                                CGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTT                                                                                                                    <  SRR19973069.45823/150‑1 (MQ=60)
                                CGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTT                                                                                                                    >  SRR19973069.1198360/1‑150 (MQ=60)
                                CGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGCTCCGNT                                                                                                                    <  SRR19973069.2538288/150‑1 (MQ=60)
                                CGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATAGACCACCTCGGCAACCGTCGTATCAGTTCCGTT                                                                                                                    >  SRR19973069.2991140/1‑150 (MQ=60)
                                CGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCAGTGATGTTATGAAAAAGCTCATCGATATCCGTAACTGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATACGTTCCGTT                                                                                                                    >  SRR19973069.716150/1‑150 (MQ=60)
                                     TCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGA                                                                                                               >  SRR19973069.3293238/1‑150 (MQ=60)
                                     TCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGA                                                                                                               >  SRR19973069.3294313/1‑150 (MQ=60)
                                        GCTGCGCGAAGAATTCGTAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAAT                                                                                                            <  SRR19973069.2113621/150‑1 (MQ=60)
                                        GCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAAT                                                                                                            <  SRR19973069.1825062/150‑1 (MQ=60)
                                        GCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAAT                                                                                                            <  SRR19973069.1451917/150‑1 (MQ=60)
                                              CGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGA                                                                                                      >  SRR19973069.1309448/1‑150 (MQ=60)
                                               GAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAA                                                                                                     >  SRR19973069.2770806/1‑150 (MQ=60)
                                                   AAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACC                                                                                                 <  SRR19973069.192364/150‑1 (MQ=60)
                                                    AATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCA                                                                                                >  SRR19973069.2833120/1‑150 (MQ=60)
                                                      TCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGT                                                                                              <  SRR19973069.2300140/150‑1 (MQ=60)
                                                       CGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTT                                                                                             <  SRR19973069.2932421/150‑1 (MQ=60)
                                                       CGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTT                                                                                             >  SRR19973069.3227112/1‑150 (MQ=60)
                                                         AAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCC                                                                                           <  SRR19973069.2549798/150‑1 (MQ=60)
                                                         AAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCC                                                                                           <  SRR19973069.2206737/150‑1 (MQ=60)
                                                          AGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCG                                                                                          >  SRR19973069.3488080/1‑150 (MQ=60)
                                                           GGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGC                                                                                         >  SRR19973069.3025290/1‑150 (MQ=60)
                                                              TCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTT                                                                                      <  SRR19973069.1117689/150‑1 (MQ=60)
                                                              TCCGGTATCCTGAGCAAAGACGACATCATTGATGTNATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTT                                                                                      <  SRR19973069.3811292/150‑1 (MQ=60)
                                                               CCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTG                                                                                     <  SRR19973069.1884213/150‑1 (MQ=60)
                                                                 GGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGC                                                                                   >  SRR19973069.3294972/1‑150 (MQ=60)
                                                                   TATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCT                                                                                 >  SRR19973069.3005269/1‑150 (MQ=60)
                                                                   TATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCT                                                                                 >  SRR19973069.3004738/1‑150 (MQ=60)
                                                                   TATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACAACCTCGGCAACCTTAGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCT                                                                                 <  SRR19973069.3770301/150‑1 (MQ=60)
                                                                    ATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTG                                                                                >  SRR19973069.394765/1‑150 (MQ=60)
                                                                    ATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTG                                                                                >  SRR19973069.394744/1‑150 (MQ=60)
                                                                    ATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTG                                                                                >  SRR19973069.1781154/1‑150 (MQ=60)
                                                                          AGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGT                                                                          >  SRR19973069.391203/1‑150 (MQ=60)
                                                                          AGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGT                                                                          >  SRR19973069.1489235/1‑150 (MQ=60)
                                                                          AGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGT                                                                          >  SRR19973069.1838809/1‑150 (MQ=60)
                                                                          AGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGT                                                                          >  SRR19973069.1838847/1‑150 (MQ=60)
                                                                            CAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGT                                                                        <  SRR19973069.993152/150‑1 (MQ=60)
                                                                            CAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGT                                                                        <  SRR19973069.992985/150‑1 (MQ=60)
                                                                                  CGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCG                                                                  >  SRR19973069.1220207/1‑150 (MQ=60)
                                                                                   GACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGT                                                                 >  SRR19973069.2174616/1‑150 (MQ=60)
                                                                                      ATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCG                                                              <  SRR19973069.150774/150‑1 (MQ=60)
                                                                                      ATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCG                                                              <  SRR19973069.151139/150‑1 (MQ=60)
                                                                                       TCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGG                                                             >  SRR19973069.2688701/1‑150 (MQ=60)
                                                                                         ATTGATTTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGAGAAGTCGATGATATAGTCCAGCTCGGCAAACGTCGTATCCGTTCCGTTGGAGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTG                                                           <  SRR19973069.669371/150‑1 (MQ=60)
                                                                                             ATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAG                                                       <  SRR19973069.2087854/150‑1 (MQ=60)
                                                                                              TGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGA                                                      <  SRR19973069.1941201/150‑1 (MQ=60)
                                                                                                   TGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTC                                                 >  SRR19973069.1577171/1‑150 (MQ=60)
                                                                                                     AAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTG                                               >  SRR19973069.672996/1‑150 (MQ=60)
                                                                                                        AAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCT                                            >  SRR19973069.1102846/1‑150 (MQ=60)
                                                                                                          GCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCT                                          <  SRR19973069.562079/150‑1 (MQ=60)
                                                                                                            TCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGG                                        <  SRR19973069.2269490/150‑1 (MQ=60)
                                                                                                            TCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGG                                        <  SRR19973069.329613/150‑1 (MQ=60)
                                                                                                            TCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTCCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGG                                        >  SRR19973069.2521077/1‑150 (MQ=60)
                                                                                                              ATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGC                                      >  SRR19973069.1319477/1‑150 (MQ=60)
                                                                                                                CGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGA                                    >  SRR19973069.3268237/1‑150 (MQ=60)
                                                                                                                  ATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATC                                  <  SRR19973069.404947/150‑1 (MQ=60)
                                                                                                                  ATAGCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATC                                  <  SRR19973069.404841/150‑1 (MQ=60)
                                                                                                                   TATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCT                                 <  SRR19973069.1133821/150‑1 (MQ=60)
                                                                                                                   TATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCT                                 <  SRR19973069.1824724/150‑1 (MQ=60)
                                                                                                                   TATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCT                                 >  SRR19973069.2431111/1‑150 (MQ=60)
                                                                                                                        GTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATA                            >  SRR19973069.3200600/1‑150 (MQ=60)
                                                                                                                           ACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCC                         <  SRR19973069.2155929/150‑1 (MQ=60)
                                                                                                                             GGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTG                       >  SRR19973069.2192910/1‑150 (MQ=60)
                                                                                                                             GGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTG                       >  SRR19973069.1970591/1‑150 (MQ=60)
                                                                                                                             GGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTG                       >  SRR19973069.2036305/1‑150 (MQ=60)
                                                                                                                             GGTAAAGGCGAAGTCGATGATATAGAACACCTCGGCAACCGTAGTATACGTTCAGTTGGCGAAAATGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGACTATCTCTGGGCGAGCTTGATACCCTG                       >  SRR19973069.26508/1‑150 (MQ=60)
                                                                                                                               TAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGAT                     >  SRR19973069.639598/1‑150 (MQ=60)
                                                                                                                                AAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATG                    >  SRR19973069.1972998/1‑150 (MQ=60)
                                                                                                                                AAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATG                    >  SRR19973069.1972842/1‑150 (MQ=60)
                                                                                                                                 AAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGC                   >  SRR19973069.2118906/1‑150 (MQ=60)
                                                                                                                                     CGAAGTCGATGATATAGACCACCTCGGAAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACA               <  SRR19973069.1293203/150‑1 (MQ=60)
                                                                                                                                     CGAAGTCGATGATATAGACCACCTCGGAAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACA               <  SRR19973069.1293186/150‑1 (MQ=60)
                                                                                                                                       AAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGG             <  SRR19973069.879643/150‑1 (MQ=60)
                                                                                                                                       AAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGG             <  SRR19973069.879637/150‑1 (MQ=60)
                                                                                                                                       AAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGG             <  SRR19973069.2213409/150‑1 (MQ=60)
                                                                                                                                       AAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGG             <  SRR19973069.2215822/150‑1 (MQ=60)
                                                                                                                                        AGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGA            >  SRR19973069.1170595/1‑150 (MQ=60)
                                                                                                                                        AGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGA            >  SRR19973069.2149898/1‑150 (MQ=60)
                                                                                                                                         GTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGAT           <  SRR19973069.1785947/150‑1 (MQ=60)
                                                                                                                                              TGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGAT      <  SRR19973069.3490884/150‑1 (MQ=60)
                                                                                                                                              TGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGAT      <  SRR19973069.1564798/150‑1 (MQ=60)
                                                                                                                                              TGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGAT      <  SRR19973069.182109/150‑1 (MQ=60)
                                                                                                                                               GATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATC     >  SRR19973069.20143/1‑150 (MQ=60)
                                                                                                                                               GATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATC     >  SRR19973069.924250/1‑150 (MQ=60)
                                                                                                                                                ATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCA    >  SRR19973069.1542325/1‑150 (MQ=60)
                                                                                                                                                ATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCA    >  SRR19973069.1542410/1‑150 (MQ=60)
                                                                                                                                                ATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCA    >  SRR19973069.3090570/1‑150 (MQ=60)
                                                                                                                                                  ATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAAC  <  SRR19973069.5293/150‑1 (MQ=60)
                                                                                                                                                  ATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAAC  <  SRR19973069.5325/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
ACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAAC  >  CP009273/4172359‑4172654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑