Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,172,507 C→A 100% I445I (ATC→ATA)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,172,5070CA96.7% 649.5 / 11.5 213I445I (ATC→ATA) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (3/4);  new base A (117/89);  total (120/93)
Fisher's exact test for biased strand distribution p-value = 7.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 213 total aligned reads displayed.

TGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATG  >  CP009273/4172362‑4172648
                                                                                                                                                 |                                                                                                                                             
tGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGa                                                                                                                                             <  1:3228551/148‑1 (MQ=255)
  tCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTTAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGacc                                                                                                                                           <  2:1645714/148‑1 (MQ=255)
  tCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGacc                                                                                                                                           <  2:2262103/148‑1 (MQ=255)
     gCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGaccacc                                                                                                                                        <  1:1538528/148‑1 (MQ=255)
      cGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCt                                                                                                                                       <  2:2652715/148‑1 (MQ=255)
             tCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAAc                                                                                                                                <  2:2476363/148‑1 (MQ=255)
                tATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACcgt                                                                                                                             <  1:922222/148‑1 (MQ=255)
                  tGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACcgtcg                                                                                                                           >  1:1183736/1‑148 (MQ=255)
                  tGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACcgtcg                                                                                                                           >  2:1181696/1‑148 (MQ=255)
                  tGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACcgtcg                                                                                                                           >  2:1898729/1‑148 (MQ=255)
                   gAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACcgtcgt                                                                                                                          >  1:1168434/1‑148 (MQ=255)
                   gAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACcgtcgt                                                                                                                          <  1:949961/148‑1 (MQ=255)
                   gAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACcgtcgt                                                                                                                          >  2:2641131/1‑148 (MQ=255)
                   gAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACcgtcgt                                                                                                                          >  2:3105187/1‑148 (MQ=255)
                    aaGTTCAATCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTa                                                                                                                         >  1:1065482/1‑148 (MQ=255)
                     aGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAt                                                                                                                        <  1:2996560/148‑1 (MQ=255)
                       ttCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAtcc                                                                                                                      <  2:1100325/148‑1 (MQ=255)
                        tCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAtccg                                                                                                                     >  1:2175245/1‑148 (MQ=255)
                          aaCCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAtccgtt                                                                                                                   >  2:3342834/1‑148 (MQ=255)
                                tctcTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACAACCTCGGCAACCGTCGTATCCGTTCCGTTg                                                                                                             >  2:1976661/1‑148 (MQ=255)
                                 ctctGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTgg                                                                                                            >  2:1588340/1‑148 (MQ=255)
                                 ctctGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTgg                                                                                                            <  2:1269585/148‑1 (MQ=255)
                                   ctgctgCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCg                                                                                                          >  2:455622/1‑148 (MQ=255)
                                   ctgctgCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCg                                                                                                          >  1:132852/1‑148 (MQ=255)
                                     gctgcGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGaa                                                                                                        >  1:725354/1‑148 (MQ=255)
                                     gctgcGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGaa                                                                                                        <  1:2562756/148‑1 (MQ=255)
                                       tgcGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAAt                                                                                                      <  1:2645033/148‑1 (MQ=255)
                                         cgcgAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATgg                                                                                                    <  2:2737779/148‑1 (MQ=255)
                                         cgcgAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATgg                                                                                                    >  1:1785900/1‑148 (MQ=255)
                                             aagaaATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGa                                                                                                >  2:2987567/1‑148 (MQ=255)
                                               gaaATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGaaa                                                                                              >  1:159194/1‑148 (MQ=255)
                                                 aaTCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAAc                                                                                            <  2:1275885/148‑1 (MQ=255)
                                                 aaTCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAAc                                                                                            <  2:1276999/148‑1 (MQ=255)
                                                 aaTCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAAc                                                                                            <  2:870201/148‑1 (MQ=255)
                                                 aaTCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAAc                                                                                            >  2:772560/1‑148 (MQ=255)
                                                  aTCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAAcc                                                                                           <  1:3442430/148‑1 (MQ=255)
                                                  aTCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAAcc                                                                                           >  2:2551207/1‑148 (MQ=255)
                                                    cGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAg                                                                                         >  2:1901666/1‑148 (MQ=255)
                                                    cGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAg                                                                                         >  2:3298921/1‑148 (MQ=255)
                                                     gAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGt                                                                                        <  1:1769788/148‑1 (MQ=255)
                                                     gAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGt                                                                                        <  1:1663357/148‑1 (MQ=255)
                                                     gAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGt                                                                                        <  1:3111534/148‑1 (MQ=255)
                                                      aaGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGtt                                                                                       >  1:2000574/1‑148 (MQ=255)
                                                      aaGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGtt                                                                                       >  1:1024068/1‑148 (MQ=255)
                                                        ggTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTcc                                                                                     >  2:774031/1‑148 (MQ=255)
                                                        ggTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGCCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTcc                                                                                     >  2:2656549/1‑148 (MQ=255)
                                                              ggTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTg                                                                               >  2:2994858/1‑148 (MQ=255)
                                                                 aTCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGcc                                                                            >  1:2151451/1‑148 (MQ=255)
                                                                 aTCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGcc                                                                            <  1:1465608/148‑1 (MQ=255)
                                                                  tCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCt                                                                           <  2:2998450/148‑1 (MQ=255)
                                                                  tCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCt                                                                           <  1:3347464/148‑1 (MQ=255)
                                                                      gAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTa                                                                       >  2:1682122/1‑148 (MQ=255)
                                                                       aGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTAc                                                                      >  2:2389636/1‑148 (MQ=255)
                                                                       aGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTAc                                                                      >  2:2603460/1‑148 (MQ=255)
                                                                        gCAAAGACGACATCATTTATGTTATGAAAAAGCTCATCTATATCCGTAACGGTAAATGCGAAGTCGATGATATAGACCACCTCGGCAAACGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACg                                                                     <  1:2491288/148‑1 (MQ=255)
                                                                         cAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACgt                                                                    <  1:3190449/148‑1 (MQ=255)
                                                                         cAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACgt                                                                    >  2:712925/1‑148 (MQ=255)
                                                                          aaaGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACgtg                                                                   <  2:2177191/148‑1 (MQ=255)
                                                                            aGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTa                                                                 >  2:1643674/1‑148 (MQ=255)
                                                                              acgacATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTaga                                                               >  1:675690/1‑148 (MQ=255)
                                                                               cgacATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTagag                                                              <  1:1834123/148‑1 (MQ=255)
                                                                                gacATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGc                                                             <  1:348132/148‑1 (MQ=255)
                                                                                 acATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCg                                                            >  2:523346/1‑148 (MQ=255)
                                                                                      atTGATGTTATGAACAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCgg                                                       <  1:2684168/148‑1 (MQ=255)
                                                                                      atTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAGAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCgg                                                       <  2:1776627/148‑1 (MQ=255)
                                                                                      atTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCgg                                                       <  1:3421535/148‑1 (MQ=255)
                                                                                      atTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCgg                                                       <  2:3539364/148‑1 (MQ=255)
                                                                                       ttGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGt                                                      >  2:1123401/1‑148 (MQ=255)
                                                                                       ttGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGt                                                      >  2:1158116/1‑148 (MQ=255)
                                                                                       ttGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGt                                                      >  2:1450571/1‑148 (MQ=255)
                                                                                            gTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAag                                                 >  2:3219142/1‑148 (MQ=255)
                                                                                            gTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAag                                                 >  2:2925802/1‑148 (MQ=255)
                                                                                            gTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAag                                                 >  2:1021079/1‑148 (MQ=255)
                                                                                             ttATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAaga                                                <  1:1246024/148‑1 (MQ=255)
                                                                                              tATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAagag                                               <  2:583708/148‑1 (MQ=255)
                                                                                                tGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCg                                             >  1:1032658/1‑148 (MQ=255)
                                                                                                tGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCg                                             >  1:2704619/1‑148 (MQ=255)
                                                                                                 gAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgt                                            >  1:2653773/1‑148 (MQ=255)
                                                                                                 gAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgt                                            >  1:2545817/1‑148 (MQ=255)
                                                                                                 gAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgt                                            >  1:649632/1‑148 (MQ=255)
                                                                                                  aaaaaGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgtc                                           >  1:1134566/1‑148 (MQ=255)
                                                                                                   aaaaGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgtct                                          >  2:2162422/1‑148 (MQ=255)
                                                                                                     aaGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgtctgt                                        >  1:2968739/1‑148 (MQ=255)
                                                                                                        cTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGtctc                                     >  2:2831886/1‑148 (MQ=255)
                                                                                                        cTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGtctc                                     <  1:1898729/148‑1 (MQ=255)
                                                                                                        cTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGtctc                                     <  2:1947585/148‑1 (MQ=255)
                                                                                                            tCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTggg                                 >  2:3009981/1‑148 (MQ=255)
                                                                                                               atatCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGa                              >  1:281542/1‑148 (MQ=255)
                                                                                                               atatCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGa                              >  2:2797566/1‑148 (MQ=255)
                                                                                                                tatCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGAt                             <  1:2937265/148‑1 (MQ=255)
                                                                                                                 atCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATc                            <  2:156665/148‑1 (MQ=255)
                                                                                                                       aaCGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACTGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATa                      <  2:655/148‑1 (MQ=255)
                                                                                                                         cGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATAcc                    >  1:1929056/1‑148 (MQ=255)
                                                                                                                         cGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATAcc                    <  1:1995773/148‑1 (MQ=255)
                                                                                                                          ggTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATAccc                   <  2:2776438/148‑1 (MQ=255)
                                                                                                                                ggCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGc             <  2:76903/148‑1 (MQ=255)
                                                                                                                                      gTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAgg       <  2:1349533/148‑1 (MQ=255)
                                                                                                                                        cGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGat     >  2:2004704/1‑148 (MQ=255)
                                                                                                                                           tgatATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATg  >  2:1816448/1‑148 (MQ=255)
                                                                                                                                           tgatATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATg  >  1:48168/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
TGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATG  >  CP009273/4172362‑4172648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACG  >  CP009273/4172359‑4172655
                                                                                                                                                    |                                                                                                                                                    
ACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAG                                                                                                                                                     <  SRR19973066.2668983/150‑1 (MQ=60)
ACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAG                                                                                                                                                     <  SRR19973066.871057/150‑1 (MQ=60)
  TTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGAC                                                                                                                                                   <  SRR19973066.1795124/150‑1 (MQ=60)
  TTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGAC                                                                                                                                                   <  SRR19973066.2448287/150‑1 (MQ=60)
  TTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGAC                                                                                                                                                   >  SRR19973066.1764736/1‑150 (MQ=60)
   TGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACC                                                                                                                                                  <  SRR19973066.1466428/150‑1 (MQ=60)
   TGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACC                                                                                                                                                  <  SRR19973066.136330/150‑1 (MQ=60)
   TGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACC                                                                                                                                                  <  SRR19973066.3249842/150‑1 (MQ=60)
   TGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACC                                                                                                                                                  <  SRR19973066.3249887/150‑1 (MQ=60)
        GCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTC                                                                                                                                             <  SRR19973066.1549512/150‑1 (MQ=60)
                   TATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCG                                                                                                                                  >  SRR19973066.1192047/1‑150 (MQ=60)
                   TATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGGATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCG                                                                                                                                  <  SRR19973066.928869/150‑1 (MQ=60)
                    ATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGT                                                                                                                                 >  SRR19973066.1176870/1‑150 (MQ=60)
                    ATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGT                                                                                                                                 >  SRR19973066.1176696/1‑150 (MQ=60)
                     TGAAGTTCAATCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTA                                                                                                                                >  SRR19973066.1073294/1‑150 (MQ=60)
                     TGAAGTTCAATCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTA                                                                                                                                >  SRR19973066.1071466/1‑150 (MQ=60)
                      GAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAT                                                                                                                               <  SRR19973066.956904/150‑1 (MQ=60)
                        AGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCC                                                                                                                             <  SRR19973066.1940463/150‑1 (MQ=60)
                        AGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCC                                                                                                                             <  SRR19973066.1940468/150‑1 (MQ=60)
                        AGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCC                                                                                                                             <  SRR19973066.3016443/150‑1 (MQ=60)
                         GTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCG                                                                                                                            >  SRR19973066.2190353/1‑150 (MQ=60)
                                CGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTT                                                                                                                     <  SRR19973066.982500/150‑1 (MQ=60)
                                    CTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCG                                                                                                                 >  SRR19973066.134151/1‑150 (MQ=60)
                                      CTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAA                                                                                                               >  SRR19973066.730730/1‑150 (MQ=60)
                                        GCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAAT                                                                                                             <  SRR19973066.2580151/150‑1 (MQ=60)
                                        GCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAAT                                                                                                             <  SRR19973066.2580349/150‑1 (MQ=60)
                                         CTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATG                                                                                                            >  SRR19973066.1206622/1‑150 (MQ=60)
                                          TGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGG                                                                                                           >  SRR19973066.1798639/1‑150 (MQ=60)
                                          TGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGG                                                                                                           <  SRR19973066.2663455/150‑1 (MQ=60)
                                          TGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGG                                                                                                           <  SRR19973066.2662807/150‑1 (MQ=60)
                                              CGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGA                                                                                                       >  SRR19973066.2178086/1‑150 (MQ=60)
                                               GAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAA                                                                                                      >  SRR19973066.690504/1‑150 (MQ=60)
                                                AAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAA                                                                                                     >  SRR19973066.160653/1‑150 (MQ=60)
                                                    AATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCA                                                                                                 <  SRR19973066.2025956/150‑1 (MQ=60)
                                                     ATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAG                                                                                                <  SRR19973066.3465459/150‑1 (MQ=60)
                                                       CGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTT                                                                                              <  SRR19973066.2268328/150‑1 (MQ=60)
                                                       CGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTT                                                                                              <  SRR19973066.1658531/150‑1 (MQ=60)
                                                       CGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTT                                                                                              >  SRR19973066.1031606/1‑150 (MQ=60)
                                                       CGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTT                                                                                              >  SRR19973066.2014633/1‑150 (MQ=60)
                                                        GAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTC                                                                                             <  SRR19973066.1782467/150‑1 (MQ=60)
                                                        GAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTC                                                                                             <  SRR19973066.1675474/150‑1 (MQ=60)
                                                        GAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTC                                                                                             <  SRR19973066.3132316/150‑1 (MQ=60)
                                                                  GTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCC                                                                                   >  SRR19973066.2166385/1‑150 (MQ=60)
                                                                    ATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTG                                                                                 <  SRR19973066.1475953/150‑1 (MQ=60)
                                                                    ATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTG                                                                                 <  SRR19973066.1039239/150‑1 (MQ=60)
                                                                     TCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGG                                                                                <  SRR19973066.3369751/150‑1 (MQ=60)
                                                                      CCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGT                                                                               >  SRR19973066.2990017/1‑150 (MQ=60)
                                                                       CTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTA                                                                              >  SRR19973066.2717518/1‑150 (MQ=60)
                                                                        TGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTAC                                                                             >  SRR19973066.948924/1‑150 (MQ=60)
                                                                           GCAAAGACGACATCATTTATGTTATGAAAAAGCTCATCTATATCCGTAACGGTAAATGCGAAGTCGATGATATAGACCACCTCGGCAAACGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTG                                                                          <  SRR19973066.2508280/150‑1 (MQ=60)
                                                                            CAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGT                                                                         <  SRR19973066.3211654/150‑1 (MQ=60)
                                                                             AAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTA                                                                        >  SRR19973066.1307039/1‑150 (MQ=60)
                                                                             AAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTA                                                                        >  SRR19973066.2963846/1‑150 (MQ=60)
                                                                             AAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTA                                                                        >  SRR19973066.1799789/1‑150 (MQ=60)
                                                                              AAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAG                                                                       >  SRR19973066.1078033/1‑150 (MQ=60)
                                                                               AGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGA                                                                      >  SRR19973066.680447/1‑150 (MQ=60)
                                                                               AGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGA                                                                      >  SRR19973066.680633/1‑150 (MQ=60)
                                                                                  CGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCG                                                                   <  SRR19973066.1847221/150‑1 (MQ=60)
                                                                                   GACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGT                                                                  <  SRR19973066.350800/150‑1 (MQ=60)
                                                                                   GACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGT                                                                  <  SRR19973066.350844/150‑1 (MQ=60)
                                                                                    ACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTG                                                                 <  SRR19973066.2547276/150‑1 (MQ=60)
                                                                                      ATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCG                                                               <  SRR19973066.1539406/150‑1 (MQ=60)
                                                                                         ATTGATGTTATGAACAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTG                                                            <  SRR19973066.2702076/150‑1 (MQ=60)
                                                                                         ATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTG                                                            <  SRR19973066.2700210/150‑1 (MQ=60)
                                                                                         ATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTG                                                            <  SRR19973066.3444418/150‑1 (MQ=60)
                                                                                          TTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGA                                                           >  SRR19973066.1164455/1‑150 (MQ=60)
                                                                                                TTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGC                                                     <  SRR19973066.1254960/150‑1 (MQ=60)
                                                                                                TTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGC                                                     <  SRR19973066.2568535/150‑1 (MQ=60)
                                                                                                 TATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCG                                                    >  SRR19973066.2722745/1‑150 (MQ=60)
                                                                                                 TATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCG                                                    >  SRR19973066.1040258/1‑150 (MQ=60)
                                                                                                  ATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGT                                                   >  SRR19973066.654344/1‑150 (MQ=60)
                                                                                                  ATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGT                                                   >  SRR19973066.2563113/1‑150 (MQ=60)
                                                                                                  ATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGT                                                   >  SRR19973066.2671577/1‑150 (MQ=60)
                                                                                                   TGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTC                                                  >  SRR19973066.1142699/1‑150 (MQ=60)
                                                                                                    GAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCT                                                 >  SRR19973066.3073249/1‑150 (MQ=60)
                                                                                                     AAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTG                                                <  SRR19973066.3460514/150‑1 (MQ=60)
                                                                                                     AAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTG                                                <  SRR19973066.2658896/150‑1 (MQ=60)
                                                                                                      AAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGT                                               >  SRR19973066.299111/1‑150 (MQ=60)
                                                                                                      AAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGT                                               >  SRR19973066.2988368/1‑150 (MQ=60)
                                                                                                      AAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGT                                               >  SRR19973066.1558649/1‑150 (MQ=60)
                                                                                                      AAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGT                                               >  SRR19973066.603162/1‑150 (MQ=60)
                                                                                                        AAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCT                                             >  SRR19973066.399467/1‑150 (MQ=60)
                                                                                                         AGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTC                                            >  SRR19973066.1790968/1‑150 (MQ=60)
                                                                                                         AGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTC                                            >  SRR19973066.3403298/1‑150 (MQ=60)
                                                                                                           CTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTG                                          <  SRR19973066.3201620/150‑1 (MQ=60)
                                                                                                           CTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTG                                          <  SRR19973066.1912276/150‑1 (MQ=60)
                                                                                                                CGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGA                                     >  SRR19973066.283589/1‑150 (MQ=60)
                                                                                                                 GATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGAT                                    >  SRR19973066.2768267/1‑150 (MQ=60)
                                                                                                                 GATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGAT                                    <  SRR19973066.2838832/150‑1 (MQ=60)
                                                                                                                  ATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATC                                   >  SRR19973066.189016/1‑150 (MQ=60)
                                                                                                                   TATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCT                                  <  SRR19973066.2956800/150‑1 (MQ=60)
                                                                                                                         TAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATAC                            <  SRR19973066.1971368/150‑1 (MQ=60)
                                                                                                                          AACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACC                           >  SRR19973066.1942763/1‑150 (MQ=60)
                                                                                                                            CGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCT                         <  SRR19973066.2010874/150‑1 (MQ=60)
                                                                                                                            CGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCT                         <  SRR19973066.2009812/150‑1 (MQ=60)
                                                                                                                            CGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCT                         >  SRR19973066.3325321/1‑150 (MQ=60)
                                                                                                                                      GAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAG               >  SRR19973066.863514/1‑150 (MQ=60)
                                                                                                                                          TCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATA           >  SRR19973066.472348/1‑150 (MQ=60)
                                                                                                                                            GATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATG         >  SRR19973066.48784/1‑150 (MQ=60)
                                                                                                                                                  ATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAAC   >  SRR19973066.981543/1‑150 (MQ=60)
                                                                                                                                                   TAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACG  >  SRR19973066.3498355/1‑150 (MQ=60)
                                                                                                                                                   TAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACG  >  SRR19973066.3499684/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
ACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACG  >  CP009273/4172359‑4172655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑