Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,172,507 C→A 100% I445I (ATC→ATA)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,172,5070CA97.4% 851.2 / 9.0 269I445I (ATC→ATA) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (3/4);  new base A (144/118);  total (147/122)
Fisher's exact test for biased strand distribution p-value = 7.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 271 total aligned reads displayed.

TGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAA  >  CP009273/4172362‑4172653
                                                                                                                                                 |                                                                                                                                                  
 gTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGacca                                                                                                                                               >  2:3388517/1‑150 (MQ=255)
  tCTGCGGTTGGTCGGATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTAGGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCTATGATATAGaccac                                                                                                                                              >  2:2983497/1‑150 (MQ=255)
   cTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGaccacc                                                                                                                                             >  2:91920/1‑150 (MQ=255)
   cTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGaccacc                                                                                                                                             >  2:4284815/1‑150 (MQ=255)
      cGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCg                                                                                                                                          >  1:386445/1‑150 (MQ=255)
           ggTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAAc                                                                                                                                     >  1:977078/1‑150 (MQ=255)
            gTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAAcc                                                                                                                                    <  2:5067270/150‑1 (MQ=255)
               gTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAACAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACcgtc                                                                                                                                 <  2:4121294/150‑1 (MQ=255)
                 aTGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACcgtcgt                                                                                                                               >  2:1730950/1‑150 (MQ=255)
                  tGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTa                                                                                                                              <  1:508442/150‑1 (MQ=255)
                   gAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAt                                                                                                                             <  2:3509507/150‑1 (MQ=255)
                    aaGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAtc                                                                                                                            >  1:798487/1‑150 (MQ=255)
                    aaGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAtc                                                                                                                            <  1:4719973/150‑1 (MQ=255)
                     aGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCACAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAtcc                                                                                                                           >  2:4789910/1‑150 (MQ=255)
                      gTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAtccg                                                                                                                          >  2:4620756/1‑150 (MQ=255)
                      gTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAtccg                                                                                                                          >  2:2740993/1‑150 (MQ=255)
                      gTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAtccg                                                                                                                          >  1:2315217/1‑150 (MQ=255)
                      gTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAtccg                                                                                                                          <  2:5012847/150‑1 (MQ=255)
                         cAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAtccgttc                                                                                                                       >  2:773850/1‑150 (MQ=255)
                            ccGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAtccgttccgt                                                                                                                    <  2:664163/150‑1 (MQ=255)
                               ttCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGAGATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTgg                                                                                                                 >  2:4717256/1‑150 (MQ=255)
                                tctcTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGc                                                                                                                >  1:1101346/1‑150 (MQ=255)
                                 ctctGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCg                                                                                                               <  1:623221/150‑1 (MQ=255)
                                 ctctGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCg                                                                                                               >  2:215441/1‑150 (MQ=255)
                                  tctGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGa                                                                                                              <  2:2430765/150‑1 (MQ=255)
                                   ctgctgCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGaa                                                                                                             <  2:2905368/150‑1 (MQ=255)
                                   ctgctgCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGaa                                                                                                             >  1:3488546/1‑150 (MQ=255)
                                    tgctgcGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGaaa                                                                                                            <  1:1264320/150‑1 (MQ=255)
                                    tgctgcGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGaaa                                                                                                            >  2:779285/1‑150 (MQ=255)
                                     gctgcGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAAt                                                                                                           <  1:3120286/150‑1 (MQ=255)
                                      ctgcGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATg                                                                                                          >  1:306567/1‑150 (MQ=255)
                                       tgcGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAAAGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATgg                                                                                                         <  2:1445392/150‑1 (MQ=255)
                                         cgcgAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCg                                                                                                       >  2:2124587/1‑150 (MQ=255)
                                           cgAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGa                                                                                                     >  1:1823039/1‑150 (MQ=255)
                                           cgAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGa                                                                                                     <  1:3306920/150‑1 (MQ=255)
                                            gaagaaATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGaa                                                                                                    >  2:3583511/1‑150 (MQ=255)
                                             aagatATCGACGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGaaa                                                                                                   <  2:4332852/150‑1 (MQ=255)
                                             aagaaATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGaaa                                                                                                   >  1:5156367/1‑150 (MQ=255)
                                              agaaATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGaaaa                                                                                                  >  1:1869768/1‑150 (MQ=255)
                                              agaaATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACCGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGACGTATCCGTTCCGTTGGCGAAATGGCGGaaaa                                                                                                  >  2:2665577/1‑150 (MQ=255)
                                                   tCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGt                                                                                             >  2:3576179/1‑150 (MQ=255)
                                                    cGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGtt                                                                                            <  2:1042668/150‑1 (MQ=255)
                                                     gAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTc                                                                                           <  2:2998983/150‑1 (MQ=255)
                                                       aGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTcca                                                                                         <  2:1575459/150‑2 (MQ=255)
                                                         gTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCcgcg                                                                                       <  1:552521/150‑1 (MQ=255)
                                                         gTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCcgcg                                                                                       <  2:1228726/150‑1 (MQ=255)
                                                          ttCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGt                                                                                      >  1:3397014/1‑150 (MQ=255)
                                                                 aTCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTg                                                                               >  2:2437598/1‑150 (MQ=255)
                                                                 aTCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTg                                                                               >  2:1928305/1‑150 (MQ=255)
                                                                   ccTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGTTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGt                                                                             >  1:2659189/1‑150 (MQ=255)
                                                                   ccTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGt                                                                             >  1:4522768/1‑150 (MQ=255)
                                                                      gAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACg                                                                          >  2:3619938/1‑150 (MQ=255)
                                                                          aaaGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTa                                                                      >  1:2621231/1‑150 (MQ=255)
                                                                             gacgacATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTagag                                                                   >  2:3798803/1‑150 (MQ=255)
                                                                                 acATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTg                                                               <  2:3315165/150‑1 (MQ=255)
                                                                                 acATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTg                                                               <  1:1706733/150‑1 (MQ=255)
                                                                                  catcatTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGc                                                              <  2:4444000/150‑1 (MQ=255)
                                                                                       ttGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGa                                                         >  2:717514/1‑150 (MQ=255)
                                                                                        tGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGaa                                                        <  1:2543448/150‑1 (MQ=255)
                                                                                        tGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGaa                                                        >  1:1478272/1‑150 (MQ=255)
                                                                                          aTGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAag                                                      <  2:4515072/150‑1 (MQ=255)
                                                                                           tGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTCCGTGTAGAGCGTGCGGTGAAaga                                                     >  2:2562523/1‑150 (MQ=255)
                                                                                            gTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAagag                                                    >  2:1617824/1‑150 (MQ=255)
                                                                                             ttATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGc                                                   <  1:382161/150‑1 (MQ=255)
                                                                                                tGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgtc                                                >  2:639602/1‑150 (MQ=255)
                                                                                                tGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgtc                                                >  1:2800179/1‑150 (MQ=255)
                                                                                                 gAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgtct                                               >  2:2199030/1‑150 (MQ=255)
                                                                                                   aaaaGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgtctgt                                             <  1:4893556/150‑1 (MQ=255)
                                                                                                   aaaaGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgtctgt                                             <  1:1053198/150‑1 (MQ=255)
                                                                                                    aaaGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgtctgtc                                            <  1:2380345/150‑1 (MQ=255)
                                                                                                     aaGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgtctgtct                                           >  1:3814162/1‑150 (MQ=255)
                                                                                                     aaGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgtctgtct                                           <  1:4110394/150‑1 (MQ=255)
                                                                                                      aGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGtctc                                          <  1:3258472/150‑1 (MQ=255)
                                                                                                       gCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTctct                                         <  1:3576184/150‑1 (MQ=255)
                                                                                                        cTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTg                                        <  1:4489756/150‑1 (MQ=255)
                                                                                                             cGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGa                                   <  2:5120914/150‑1 (MQ=255)
                                                                                                              gATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGAt                                  >  2:3282941/1‑150 (MQ=255)
                                                                                                                tatCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCt                                <  2:2315217/150‑1 (MQ=255)
                                                                                                                tatCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCt                                >  2:374754/1‑150 (MQ=255)
                                                                                                                tatCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCt                                <  2:977078/150‑1 (MQ=255)
                                                                                                                  tCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTgg                              <  1:1450685/150‑1 (MQ=255)
                                                                                                                    cGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGAt                            >  2:2024170/1‑150 (MQ=255)
                                                                                                                    cGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACAAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGAt                            >  2:165023/1‑150 (MQ=255)
                                                                                                                      tAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATAc                          <  2:3732103/150‑1 (MQ=255)
                                                                                                                      tAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATAc                          >  1:4284535/1‑150 (MQ=255)
                                                                                                                      tAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATAc                          >  1:363261/1‑150 (MQ=255)
                                                                                                                        aCGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATAccc                        <  2:1254796/150‑1 (MQ=255)
                                                                                                                         cGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCt                       >  1:3558020/1‑150 (MQ=255)
                                                                                                                           gTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGa                     <  1:3281197/150‑1 (MQ=255)
                                                                                                                            tAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGAt                    <  1:2207653/150‑1 (MQ=255)
                                                                                                                            tAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGAt                    >  1:1654226/1‑150 (MQ=255)
                                                                                                                                  cGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCcaca              <  1:2437598/150‑1 (MQ=255)
                                                                                                                                   gAAGTCGATGATATATACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCTGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAg             >  2:1640224/1‑150 (MQ=255)
                                                                                                                                   gAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAg             >  2:1979663/1‑150 (MQ=255)
                                                                                                                                   gAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAg             >  1:891680/1‑150 (MQ=255)
                                                                                                                                   gAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAg             <  1:1752254/150‑1 (MQ=255)
                                                                                                                                    aaGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAgg            <  2:3151882/150‑1 (MQ=255)
                                                                                                                                     aGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGa           >  1:2144836/1‑150 (MQ=255)
                                                                                                                                        cGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGatat        <  2:546606/150‑1 (MQ=255)
                                                                                                                                              tataGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCaa  >  1:1397605/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAA  >  CP009273/4172362‑4172653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGC  >  CP009273/4172358‑4172656
                                                                                                                                                     |                                                                                                                                                     
GACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATA                                                                                                                                                       >  SRR19973063.3792112/1‑150 (MQ=60)
    TGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACC                                                                                                                                                   >  SRR19973063.769014/1‑150 (MQ=60)
    TGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACC                                                                                                                                                   <  SRR19973063.1813760/150‑1 (MQ=60)
    TGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACC                                                                                                                                                   <  SRR19973063.1813755/150‑1 (MQ=60)
     GTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCA                                                                                                                                                  <  SRR19973063.85856/150‑1 (MQ=60)
       CTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACC                                                                                                                                                <  SRR19973063.2704435/150‑1 (MQ=60)
          CGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGACGGTTCCGTTATCCTGATCAAAGACGACATCATTGATGTTATGATAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCG                                                                                                                                             <  SRR19973063.524216/150‑1 (MQ=60)
          CGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCG                                                                                                                                             >  SRR19973063.387544/1‑150 (MQ=60)
           GGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGG                                                                                                                                            >  SRR19973063.4917184/1‑150 (MQ=60)
           GGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGG                                                                                                                                            >  SRR19973063.2695585/1‑150 (MQ=60)
             TTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCA                                                                                                                                          <  SRR19973063.1062010/150‑1 (MQ=60)
               GGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAAC                                                                                                                                        >  SRR19973063.980023/1‑150 (MQ=60)
                  CGTATGAAGTTCAACCTTTCTCTGCTGAGCGAAGAAATCGTAGGTTCCGTTATCCTGAGCAAAGAGGAAATCATTTATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGT                                                                                                                                     <  SRR19973063.5096089/150‑1 (MQ=60)
                   GTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTC                                                                                                                                    >  SRR19973063.1466909/1‑150 (MQ=60)
                   GTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCAGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTC                                                                                                                                    <  SRR19973063.2595554/150‑1 (MQ=60)
                    TATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCG                                                                                                                                   <  SRR19973063.4880402/150‑1 (MQ=60)
                     ATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGT                                                                                                                                  <  SRR19973063.1859822/150‑1 (MQ=60)
                      TGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTA                                                                                                                                 >  SRR19973063.3610109/1‑150 (MQ=60)
                      TGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTA                                                                                                                                 <  SRR19973063.509915/150‑1 (MQ=60)
                       GAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTAT                                                                                                                                >  SRR19973063.4529567/1‑150 (MQ=60)
                        AAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATC                                                                                                                               >  SRR19973063.800790/1‑150 (MQ=60)
                        AAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATC                                                                                                                               <  SRR19973063.4735023/150‑1 (MQ=60)
                        AAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATC                                                                                                                               <  SRR19973063.2158564/150‑1 (MQ=60)
                         AGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCC                                                                                                                              <  SRR19973063.1178192/150‑1 (MQ=60)
                         AGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCC                                                                                                                              >  SRR19973063.2391392/1‑150 (MQ=60)
                         AGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCC                                                                                                                              <  SRR19973063.2341131/150‑1 (MQ=60)
                          GTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCG                                                                                                                             >  SRR19973063.2322623/1‑150 (MQ=60)
                             CAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTC                                                                                                                          >  SRR19973063.1366129/1‑150 (MQ=60)
                             CAACAGTTCTCTGCTGCGCGAAGAAATCGACGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTC                                                                                                                          <  SRR19973063.173814/150‑1 (MQ=60)
                              AACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCC                                                                                                                         <  SRR19973063.2474146/150‑1 (MQ=60)
                              AACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCC                                                                                                                         <  SRR19973063.1961993/150‑1 (MQ=60)
                               ACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCG                                                                                                                        >  SRR19973063.1477476/1‑150 (MQ=60)
                               ACCGTTCTCTGCTGCGCGAAGAAATCGAACGATCCGGAATCCTGAGAAAAGACTACATCAATGAGGTTATGAAAAAGATCATCGATAACCGTAACTGTAAAGGCGAAGACGATTATATAGACCACAACGGCAACCGTCGTATacgatacg                                                                                                                        >  SRR19973063.1857614/1‑142 (MQ=60)
                                 CGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTT                                                                                                                      <  SRR19973063.914320/150‑1 (MQ=60)
                                  GTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTG                                                                                                                     >  SRR19973063.3162015/1‑150 (MQ=60)
                                    TCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGC                                                                                                                   >  SRR19973063.3693522/1‑150 (MQ=60)
                                    TCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGC                                                                                                                   >  SRR19973063.1104701/1‑150 (MQ=60)
                                    TCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGC                                                                                                                   <  SRR19973063.3887979/150‑1 (MQ=60)
                                     CTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCG                                                                                                                  >  SRR19973063.2653506/1‑150 (MQ=60)
                                     CTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCG                                                                                                                  <  SRR19973063.625054/150‑1 (MQ=60)
                                       CTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAA                                                                                                                >  SRR19973063.3499696/1‑150 (MQ=60)
                                        TGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAA                                                                                                               <  SRR19973063.1268082/150‑1 (MQ=60)
                                         GCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAAT                                                                                                              <  SRR19973063.3130332/150‑1 (MQ=60)
                                          CTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATG                                                                                                             >  SRR19973063.307469/1‑150 (MQ=60)
                                          CTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATG                                                                                                             >  SRR19973063.307784/1‑150 (MQ=60)
                                              GCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGG                                                                                                         <  SRR19973063.5169791/150‑1 (MQ=60)
                                               CGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGA                                                                                                        >  SRR19973063.1828709/1‑150 (MQ=60)
                                               CGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGA                                                                                                        <  SRR19973063.3317496/150‑1 (MQ=60)
                                               CGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGA                                                                                                        <  SRR19973063.3317495/150‑1 (MQ=60)
                                                GAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAA                                                                                                       >  SRR19973063.143490/1‑150 (MQ=60)
                                                 AAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAA                                                                                                      >  SRR19973063.5172953/1‑150 (MQ=60)
                                                  AGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAA                                                                                                     >  SRR19973063.1875618/1‑150 (MQ=60)
                                                        CGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTT                                                                                               >  SRR19973063.721676/1‑150 (MQ=60)
                                                        CGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTT                                                                                               <  SRR19973063.2311689/150‑1 (MQ=60)
                                                        CGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTT                                                                                               >  SRR19973063.3315248/1‑150 (MQ=60)
                                                           AGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCG                                                                                            >  SRR19973063.1717330/1‑150 (MQ=60)
                                                             GTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCG                                                                                          <  SRR19973063.554033/150‑1 (MQ=60)
                                                             GTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCG                                                                                          <  SRR19973063.4145777/150‑1 (MQ=60)
                                                             GTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCG                                                                                          <  SRR19973063.554152/150‑1 (MQ=60)
                                                              TTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGT                                                                                         >  SRR19973063.3407823/1‑150 (MQ=60)
                                                               TCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTT                                                                                        <  SRR19973063.3560911/150‑1 (MQ=60)
                                                               TCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTT                                                                                        >  SRR19973063.3473684/1‑150 (MQ=60)
                                                                  GGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGC                                                                                     <  SRR19973063.4342030/150‑1 (MQ=60)
                                                                   GTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCC                                                                                    >  SRR19973063.3603897/1‑150 (MQ=60)
                                                                       CCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGTTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGT                                                                                >  SRR19973063.2667705/1‑150 (MQ=60)
                                                                       CCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGT                                                                                >  SRR19973063.4537205/1‑150 (MQ=60)
                                                                       CCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGT                                                                                >  SRR19973063.4537276/1‑150 (MQ=60)
                                                                            GCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTG                                                                           >  SRR19973063.2984591/1‑150 (MQ=60)
                                                                             CAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGT                                                                          >  SRR19973063.1647762/1‑150 (MQ=60)
                                                                              AAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTA                                                                         >  SRR19973063.2629578/1‑150 (MQ=60)
                                                                              AAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTA                                                                         >  SRR19973063.2472529/1‑150 (MQ=60)
                                                                                    GACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGT                                                                   >  SRR19973063.4623398/1‑150 (MQ=60)
                                                                                     ACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTG                                                                  <  SRR19973063.1711933/150‑1 (MQ=60)
                                                                                         CATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGT                                                              <  SRR19973063.2923479/150‑1 (MQ=60)
                                                                                            TGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAA                                                           <  SRR19973063.60152/150‑1 (MQ=60)
                                                                                            TGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAA                                                           >  SRR19973063.1482792/1‑150 (MQ=60)
                                                                                            TGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAA                                                           <  SRR19973063.2551557/150‑1 (MQ=60)
                                                                                                 TTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGC                                                      <  SRR19973063.383253/150‑1 (MQ=60)
                                                                                                    TGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTC                                                   >  SRR19973063.2809182/1‑150 (MQ=60)
                                                                                                     GAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCT                                                  >  SRR19973063.4050256/1‑150 (MQ=60)
                                                                                                       AAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGT                                                <  SRR19973063.1056387/150‑1 (MQ=60)
                                                                                                       AAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGT                                                <  SRR19973063.4909348/150‑1 (MQ=60)
                                                                                                        AAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTC                                               <  SRR19973063.2387971/150‑1 (MQ=60)
                                                                                                         AAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCT                                              <  SRR19973063.4123558/150‑1 (MQ=60)
                                                                                                         AAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCT                                              >  SRR19973063.3826301/1‑150 (MQ=60)
                                                                                                          AGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTC                                             <  SRR19973063.3268930/150‑1 (MQ=60)
                                                                                                           GCTCATCGATATCCGTTACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCT                                            <  SRR19973063.3587655/150‑1 (MQ=60)
                                                                                                           GCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCT                                            <  SRR19973063.3587650/150‑1 (MQ=60)
                                                                                                           GCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCT                                            >  SRR19973063.5172313/1‑150 (MQ=60)
                                                                                                            CTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTG                                           <  SRR19973063.4504158/150‑1 (MQ=60)
                                                                                                                   ATATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATC                                    >  SRR19973063.2191414/1‑150 (MQ=60)
                                                                                                                     ATCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTG                                  <  SRR19973063.850116/150‑1 (MQ=60)
                                                                                                                      TCCGTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGG                                 <  SRR19973063.1455110/150‑1 (MQ=60)
                                                                                                                         GTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATA                              >  SRR19973063.261562/1‑150 (MQ=60)
                                                                                                                         GTAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATA                              >  SRR19973063.118548/1‑150 (MQ=60)
                                                                                                                          TAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATAC                             >  SRR19973063.4298147/1‑150 (MQ=60)
                                                                                                                          TAACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATAC                             >  SRR19973063.364304/1‑150 (MQ=60)
                                                                                                                           AACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACC                            >  SRR19973063.1577746/1‑150 (MQ=60)
                                                                                                                           AACGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACC                            >  SRR19973063.3821815/1‑150 (MQ=60)
                                                                                                                             CGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCT                          <  SRR19973063.3874438/150‑1 (MQ=60)
                                                                                                                             CGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCT                          >  SRR19973063.2280647/1‑150 (MQ=60)
                                                                                                                             CGGTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCT                          >  SRR19973063.3569426/1‑150 (MQ=60)
                                                                                                                               GTAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGA                        <  SRR19973063.3291725/150‑1 (MQ=60)
                                                                                                                                TAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGAT                       >  SRR19973063.1659274/1‑150 (MQ=60)
                                                                                                                                TAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGAT                       >  SRR19973063.1659344/1‑150 (MQ=60)
                                                                                                                                TAAAGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGAT                       <  SRR19973063.2214607/150‑1 (MQ=60)
                                                                                                                                  AAGGCGAAGTCTTTGATATAGTCCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGATGGCCTGGTACGTGTAGAGCGAGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGC                     <  SRR19973063.3289549/150‑1 (MQ=60)
                                                                                                                                   AGGCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCC                    <  SRR19973063.3280292/150‑1 (MQ=60)
                                                                                                                                     GCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCAC                  <  SRR19973063.4295739/150‑1 (MQ=60)
                                                                                                                                     GCGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCAC                  >  SRR19973063.3165367/1‑150 (MQ=60)
                                                                                                                                      CGAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACA                 <  SRR19973063.2445389/150‑1 (MQ=60)
                                                                                                                                       GAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAG                >  SRR19973063.2425604/1‑150 (MQ=60)
                                                                                                                                       GAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAG                <  SRR19973063.1757632/150‑1 (MQ=60)
                                                                                                                                       GAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAG                >  SRR19973063.1281304/1‑150 (MQ=60)
                                                                                                                                       GAAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAG                >  SRR19973063.894269/1‑150 (MQ=60)
                                                                                                                                        AAGTCGATGATATCGACCACCTGGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGG               <  SRR19973063.34385/150‑1 (MQ=60)
                                                                                                                                        AAGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGG               >  SRR19973063.3393521/1‑150 (MQ=60)
                                                                                                                                         AGTCGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGA              >  SRR19973063.2151550/1‑150 (MQ=60)
                                                                                                                                            CGATGATATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATAT           >  SRR19973063.4630295/1‑150 (MQ=60)
                                                                                                                                                GATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATC       >  SRR19973063.3829209/1‑150 (MQ=60)
                                                                                                                                                GATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATC       >  SRR19973063.3829352/1‑150 (MQ=60)
                                                                                                                                                  TATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAA     <  SRR19973063.3549033/150‑1 (MQ=60)
                                                                                                                                                  TATAGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAA     >  SRR19973063.1401833/1‑150 (MQ=60)
                                                                                                                                                     AGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGNCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCNTGCGNTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGC  >  SRR19973063.3509527/1‑150 (MQ=60)
                                                                                                                                                     AGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGC  >  SRR19973063.3509312/1‑150 (MQ=60)
                                                                                                                                                     AGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGC  >  SRR19973063.925780/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGC  >  CP009273/4172358‑4172656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑