Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,249,117 G→T 100% intergenic (+68/‑48) dinF → / → yjbJ oxidative stress resistance protein; putative MATE family efflux pump; UV and mitomycin C inducible protein/stress‑induced protein, UPF0337 family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,249,1170GT98.4% 604.5 / ‑0.8 188intergenic (+68/‑48)dinF/yjbJoxidative stress resistance protein; putative MATE family efflux pump; UV and mitomycin C inducible protein/stress‑induced protein, UPF0337 family
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (97/88);  total (99/89)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 191 total aligned reads displayed.

TGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTG  >  CP009273/4248973‑4249261
                                                                                                                                                |                                                                                                                                                
tGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATaaa                                                                                                                                               >  1:368346/1‑148 (MQ=255)
 ggCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATaaaa                                                                                                                                              <  1:2496618/148‑1 (MQ=255)
  gCGTTGCTCGGGCTTTCTGTGGCTGCTATCTTGCGGCGTGACTGTCTCTATGGTACCTGGTTTGCCGCATCGTTACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAATTTGCTGAATAAACTTTATCTCACTTTCAGATTAAAAg                                                                                                                                             <  2:751397/148‑1 (MQ=255)
     ttGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGa                                                                                                                                          <  1:589560/148‑1 (MQ=255)
     ttGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGa                                                                                                                                          <  1:1777347/148‑1 (MQ=255)
     ttGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGa                                                                                                                                          <  2:560716/148‑1 (MQ=255)
               tttCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACtctc                                                                                                                                <  1:757067/148‑1 (MQ=255)
               tttCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACtctc                                                                                                                                <  2:1706177/148‑1 (MQ=255)
               tttCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACtctc                                                                                                                                >  2:1370473/1‑148 (MQ=255)
                ttCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCa                                                                                                                               >  2:3153380/1‑148 (MQ=255)
                 tctcTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCAt                                                                                                                              <  2:3006415/148‑1 (MQ=255)
                   tctGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTc                                                                                                                            <  2:2278010/148‑1 (MQ=255)
                   tctGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTc                                                                                                                            <  2:2277722/148‑1 (MQ=255)
                    ctGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTca                                                                                                                           >  2:3469579/1‑148 (MQ=255)
                     tGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTcac                                                                                                                          >  1:3467759/1‑148 (MQ=255)
                         ggcTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAAcc                                                                                                                      <  2:948030/148‑1 (MQ=255)
                           cTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTa                                                                                                                    <  1:808687/148‑1 (MQ=255)
                           cTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTa                                                                                                                    <  2:2610184/148‑1 (MQ=255)
                            tATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTaa                                                                                                                   <  1:2702831/148‑1 (MQ=255)
                               cTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGa                                                                                                                >  2:3060408/1‑148 (MQ=255)
                                tGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGAt                                                                                                               <  1:674567/148‑1 (MQ=255)
                                 ggcggcGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATg                                                                                                              <  1:2801964/148‑1 (MQ=255)
                                  gcggcgTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGa                                                                                                             <  1:2536031/148‑1 (MQ=255)
                                    ggcgTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAgg                                                                                                           >  2:1528455/1‑148 (MQ=255)
                                         cACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTg                                                                                                      >  1:3500920/1‑148 (MQ=255)
                                         cACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTg                                                                                                      >  1:1678901/1‑148 (MQ=255)
                                           cTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGAt                                                                                                    >  1:589647/1‑148 (MQ=255)
                                              gcgcAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTAt                                                                                                 <  2:412620/148‑1 (MQ=255)
                                                 cAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGaa                                                                                              <  2:2109185/148‑1 (MQ=255)
                                                 cAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGaa                                                                                              >  1:2732575/1‑148 (MQ=255)
                                                 cAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGaa                                                                                              <  2:606799/148‑1 (MQ=255)
                                                   aTGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATa                                                                                            >  1:1209850/1‑148 (MQ=255)
                                                   aTGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATa                                                                                            >  1:1212123/1‑148 (MQ=255)
                                                         ccTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATg                                                                                      <  2:2834896/148‑1 (MQ=255)
                                                         ccTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATg                                                                                      <  2:1879658/148‑1 (MQ=255)
                                                         ccTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATg                                                                                      >  2:1040795/1‑148 (MQ=255)
                                                          cTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGa                                                                                     >  1:2080785/1‑148 (MQ=255)
                                                           tGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGaa                                                                                    >  1:1821987/1‑148 (MQ=255)
                                                            ggTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAg                                                                                   <  2:1964408/148‑1 (MQ=255)
                                                             gTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGc                                                                                  <  1:979151/148‑1 (MQ=255)
                                                               ttGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCcg                                                                                >  1:2388051/1‑148 (MQ=255)
                                                                  ccGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTACTCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCcggcg                                                                             <  1:2604301/148‑1 (MQ=255)
                                                                   cGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCcggcgg                                                                            <  1:2168205/148‑1 (MQ=255)
                                                                     cAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTa                                                                          <  2:2513957/148‑1 (MQ=255)
                                                                     cAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTa                                                                          <  2:1980732/148‑1 (MQ=255)
                                                                     cAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGAATACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCAAACGATGAGGTCTTGATTATGAATAAAGATTAAGCcggagtaa                                                                          >  1:3246310/1‑143 (MQ=255)
                                                                             cGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGaaa                                                                  >  2:1830399/1‑148 (MQ=255)
                                                                                ttAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAg                                                               >  2:193035/1‑148 (MQ=255)
                                                                                 tAAAAATTCTGAATAAATAATCCTACGCCAACTTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTGGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGt                                                              <  2:2720561/148‑1 (MQ=255)
                                                                                 tAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGt                                                              >  2:480186/1‑148 (MQ=255)
                                                                                    aaaTTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTa                                                           <  2:1700834/148‑1 (MQ=255)
                                                                                       ttCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGACGCCGGCGGTAACTGGAAACAGTTTAAAg                                                        >  2:224641/1‑148 (MQ=255)
                                                                                        tCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAgg                                                       <  1:1590875/148‑1 (MQ=255)
                                                                                           gAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGACGCCGGCGGTAACTGGAAACAGTTTAAAGGTaa                                                    >  2:2216252/1‑148 (MQ=255)
                                                                                           gAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTaa                                                    >  2:2780131/1‑148 (MQ=255)
                                                                                            aataaataATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTaaa                                                   >  1:3061017/1‑148 (MQ=255)
                                                                                             ataaataaTCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAg                                                  >  1:1073312/1‑148 (MQ=255)
                                                                                               aaataaTCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTg                                                >  1:3446185/1‑148 (MQ=255)
                                                                                                 ataatCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGaa                                              >  2:1597886/1‑148 (MQ=255)
                                                                                                 ataatCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGaa                                              >  2:1139960/1‑148 (MQ=255)
                                                                                                  taatCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGaaa                                             <  2:717495/148‑1 (MQ=255)
                                                                                                    atCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAaga                                           >  1:1877710/1‑148 (MQ=255)
                                                                                                    atCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAaga                                           >  1:614298/1‑148 (MQ=255)
                                                                                                     tCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAagag                                          >  2:3250750/1‑148 (MQ=255)
                                                                                                      ccTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGc                                         >  2:135480/1‑148 (MQ=255)
                                                                                                      ccTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGc                                         >  2:3543622/1‑148 (MQ=255)
                                                                                                      ccTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGc                                         >  1:1636864/1‑148 (MQ=255)
                                                                                                      ccTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGc                                         >  2:388902/1‑148 (MQ=255)
                                                                                                       cTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCa                                        >  2:2926493/1‑148 (MQ=255)
                                                                                                         aaGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAAt                                      >  2:848747/1‑148 (MQ=255)
                                                                                                           gCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATgg                                    <  1:360476/148‑1 (MQ=255)
                                                                                                              aaaTTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGc                                 <  2:2753672/148‑1 (MQ=255)
                                                                                                              aaaTTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGc                                 >  1:2624481/1‑148 (MQ=255)
                                                                                                               aaTTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCa                                >  2:1204932/1‑148 (MQ=255)
                                                                                                                aTTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCaa                               <  2:1212072/148‑1 (MQ=255)
                                                                                                                aTTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCaa                               >  2:2604955/1‑148 (MQ=255)
                                                                                                                aTTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCaa                               >  2:1692511/1‑148 (MQ=255)
                                                                                                                 ttGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCaaa                              >  2:693977/1‑148 (MQ=255)
                                                                                                                 ttGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCaaa                              <  1:2913970/148‑1 (MQ=255)
                                                                                                                  tGCTGACTACACTTACTCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAAc                             <  2:1252014/148‑1 (MQ=255)
                                                                                                                  tGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAAc                             <  2:1251966/148‑1 (MQ=255)
                                                                                                                     tGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGa                          >  2:2455593/1‑148 (MQ=255)
                                                                                                                        cTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGACGCCGTCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCg                       >  2:2942744/1‑148 (MQ=255)
                                                                                                                          acacTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCgat                     >  1:1015185/1‑148 (MQ=255)
                                                                                                                           cacTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCgatg                    <  1:193035/148‑1 (MQ=255)
                                                                                                                           cacTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCgatg                    <  1:193899/148‑1 (MQ=255)
                                                                                                                            acTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCgatga                   >  1:463951/1‑148 (MQ=255)
                                                                                                                                 aTCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGata              <  2:379650/148‑1 (MQ=255)
                                                                                                                                 aTCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGata              <  2:410977/148‑1 (MQ=255)
                                                                                                                                 aTCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGata              <  2:1015185/148‑1 (MQ=255)
                                                                                                                                 aTCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGata              <  2:3320317/148‑1 (MQ=255)
                                                                                                                                       cGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGa        >  1:3312256/1‑148 (MQ=255)
                                                                                                                                          tCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCa     <  2:652391/148‑1 (MQ=255)
                                                                                                                                          tCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCa     >  2:2416660/1‑148 (MQ=255)
                                                                                                                                          tCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCa     >  2:72971/1‑148 (MQ=255)
                                                                                                                                          tCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGAAATGGGGCAAACTGACCGATGATGATATTACGATCa     >  1:1290319/1‑148 (MQ=255)
                                                                                                                                           cAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCag    >  2:1060802/1‑147 (MQ=255)
                                                                                                                                           cAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCAt    <  1:135480/148‑1 (MQ=255)
                                                                                                                                             gaataaAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTg  >  1:1867440/1‑148 (MQ=255)
                                                                                                                                             gaataaAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTg  >  1:1468372/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTG  >  CP009273/4248973‑4249261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTGAA  >  CP009273/4248968‑4249263
                                                                                                                                                     |                                                                                                                                                  
CTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAT                                                                                                                                                    <  SRR19973067.281657/150‑1 (MQ=60)
CTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAT                                                                                                                                                    >  SRR19973067.1385817/1‑150 (MQ=60)
   TCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAA                                                                                                                                                 >  SRR19973067.370906/1‑150 (MQ=60)
      GGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGT                                                                                                                                              <  SRR19973067.2513013/150‑1 (MQ=60)
        CGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGA                                                                                                                                            <  SRR19973067.1717554/150‑1 (MQ=60)
        CGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGA                                                                                                                                            >  SRR19973067.1187134/1‑150 (MQ=60)
          TTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAAC                                                                                                                                          <  SRR19973067.1789381/150‑1 (MQ=60)
          TTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAAC                                                                                                                                          <  SRR19973067.593319/150‑1 (MQ=60)
               CGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACT                                                                                                                                     >  SRR19973067.661968/1‑150 (MQ=60)
                    TTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCAT                                                                                                                                <  SRR19973067.1427941/150‑1 (MQ=60)
                    TTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCAT                                                                                                                                <  SRR19973067.762332/150‑1 (MQ=60)
                        TCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCAC                                                                                                                            >  SRR19973067.3489798/1‑150 (MQ=60)
                             CGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCT                                                                                                                       >  SRR19973067.1325656/1‑150 (MQ=60)
                              GGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTATCATTCACAACCTA                                                                                                                      >  SRR19973067.1893668/1‑150 (MQ=60)
                                CTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAAC                                                                                                                    <  SRR19973067.814247/150‑1 (MQ=60)
                                 TATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACG                                                                                                                   >  SRR19973067.2618998/1‑150 (MQ=60)
                                 TATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACG                                                                                                                   <  SRR19973067.2720230/150‑1 (MQ=60)
                                   TCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGAT                                                                                                                 >  SRR19973067.573669/1‑150 (MQ=60)
                                     TGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGA                                                                                                               <  SRR19973067.679200/150‑1 (MQ=60)
                                      GGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAG                                                                                                              <  SRR19973067.2820034/150‑1 (MQ=60)
                                       GCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGG                                                                                                             <  SRR19973067.2552639/150‑1 (MQ=60)
                                       GCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGG                                                                                                             <  SRR19973067.2552621/150‑1 (MQ=60)
                                          GCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCT                                                                                                          <  SRR19973067.79498/150‑1 (MQ=60)
                                            GTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTG                                                                                                        >  SRR19973067.610687/1‑150 (MQ=60)
                                            GTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTG                                                                                                        >  SRR19973067.3523117/1‑150 (MQ=60)
                                            GTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTG                                                                                                        >  SRR19973067.1690472/1‑150 (MQ=60)
                                              CACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGAT                                                                                                      >  SRR19973067.593406/1‑150 (MQ=60)
                                              CACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGAT                                                                                                      >  SRR19973067.593378/1‑150 (MQ=60)
                                                    CTCAATGGTACCTGGTTTGCCGCAACGTGACGTTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAA                                                                                                <  SRR19973067.3327005/150‑1 (MQ=60)
                                                    CGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAA                                                                                                >  SRR19973067.2750211/1‑150 (MQ=60)
                                                      CAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATA                                                                                              >  SRR19973067.1217818/1‑150 (MQ=60)
                                                      CAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATA                                                                                              >  SRR19973067.1220077/1‑150 (MQ=60)
                                                      CAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATA                                                                                              >  SRR19973067.1220130/1‑150 (MQ=60)
                                                       AATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAA                                                                                             >  SRR19973067.2785841/1‑150 (MQ=60)
                                                             ACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGA                                                                                       >  SRR19973067.2094576/1‑150 (MQ=60)
                                                             ACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGA                                                                                       >  SRR19973067.2094574/1‑150 (MQ=60)
                                                              CCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAC                                                                                      >  SRR19973067.1834338/1‑150 (MQ=60)
                                                                TGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGC                                                                                    >  SRR19973067.2405845/1‑150 (MQ=60)
                                                                TGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGC                                                                                    >  SRR19973067.2733099/1‑150 (MQ=60)
                                                                  GTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCG                                                                                  <  SRR19973067.985873/150‑1 (MQ=60)
                                                                  GTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAATCCG                                                                                  >  SRR19973067.2403566/1‑150 (MQ=60)
                                                                       CCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTACTCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGT                                                                             <  SRR19973067.2621230/150‑1 (MQ=60)
                                                                       CCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGT                                                                             <  SRR19973067.828962/150‑1 (MQ=60)
                                                                        CGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTA                                                                            <  SRR19973067.2182704/150‑1 (MQ=60)
                                                                        CGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGAATACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCAAACGATGAGGTCTTGATTATGAATAAAGATTAAGCCGGagtaa                                                                            >  SRR19973067.3266640/1‑145 (MQ=60)
                                                                           AACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAATCCGGCGGTAACT                                                                         >  SRR19973067.3014508/1‑150 (MQ=60)
                                                                                      TAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTT                                                              >  SRR19973067.1288250/1‑150 (MQ=60)
                                                                                          AATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAG                                                          <  SRR19973067.3491624/150‑1 (MQ=60)
                                                                                             TCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTA                                                       <  SRR19973067.1601777/150‑1 (MQ=60)
                                                                                               TGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAA                                                     >  SRR19973067.3080506/1‑150 (MQ=60)
                                                                                                GAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAG                                                    >  SRR19973067.1080673/1‑150 (MQ=60)
                                                                                                  ATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTG                                                  >  SRR19973067.3468192/1‑150 (MQ=60)
                                                                                                  ATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTG                                                  >  SRR19973067.3468115/1‑150 (MQ=60)
                                                                                                       TAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGA                                             >  SRR19973067.1890456/1‑150 (MQ=60)
                                                                                                       TAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGA                                             >  SRR19973067.1890521/1‑150 (MQ=60)
                                                                                                       TAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGA                                             >  SRR19973067.618297/1‑150 (MQ=60)
                                                                                                         ATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGC                                           <  SRR19973067.1604265/150‑1 (MQ=60)
                                                                                                         ATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGC                                           >  SRR19973067.1648128/1‑150 (MQ=60)
                                                                                                             TAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATG                                       <  SRR19973067.1522139/150‑1 (MQ=60)
                                                                                                             TAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATG                                       <  SRR19973067.1947395/150‑1 (MQ=60)
                                                                                                             TAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATG                                       >  SRR19973067.1807183/1‑150 (MQ=60)
                                                                                                                GCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGG                                    <  SRR19973067.362975/150‑1 (MQ=60)
                                                                                                                 CCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGC                                   >  SRR19973067.2641499/1‑150 (MQ=60)
                                                                                                                   AAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAA                                 >  SRR19973067.286226/1‑150 (MQ=60)
                                                                                                                      TTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACT                              >  SRR19973067.262952/1‑150 (MQ=60)
                                                                                                                      TTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACT                              <  SRR19973067.2932523/150‑1 (MQ=60)
                                                                                                                          TGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACC                          <  SRR19973067.3164065/150‑1 (MQ=60)
                                                                                                                           GACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCG                         <  SRR19973067.734692/150‑1 (MQ=60)
                                                                                                                             CTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGAT                       >  SRR19973067.1022153/1‑150 (MQ=60)
                                                                                                                             CTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGAT                       >  SRR19973067.413838/1‑150 (MQ=60)
                                                                                                                             CTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGAT                       >  SRR19973067.3435154/1‑150 (MQ=60)
                                                                                                                               ACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGA                     >  SRR19973067.467163/1‑150 (MQ=60)
                                                                                                                               ACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGA                     <  SRR19973067.1968129/150‑1 (MQ=60)
                                                                                                                               ACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGA                     >  SRR19973067.999764/1‑150 (MQ=60)
                                                                                                                                CACTTAATCTCACTTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGAT                    <  SRR19973067.194528/150‑1 (MQ=60)
                                                                                                                                CACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGAT                    <  SRR19973067.195397/150‑1 (MQ=60)
                                                                                                                                          CACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAAagatcggaagagcacacgtctg          >  SRR19973067.2233165/1‑128 (MQ=60)
                                                                                                                                          CACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGA          >  SRR19973067.3333182/1‑150 (MQ=60)
                                                                                                                                             GTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGAAATGGGGCAAACTGACCGATGATGATATTACGATCA       >  SRR19973067.1299130/1‑150 (MQ=60)
                                                                                                                                                CAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTG    >  SRR19973067.1880175/1‑150 (MQ=60)
                                                                                                                                                CAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTG    <  SRR19973067.3565986/150‑1 (MQ=60)
                                                                                                                                                CAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTG    >  SRR19973067.1673839/1‑150 (MQ=60)
                                                                                                                                                CAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTG    >  SRR19973067.1478163/1‑150 (MQ=60)
                                                                                                                                                CAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTG    <  SRR19973067.136687/150‑1 (MQ=60)
                                                                                                                                                  GAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTGAA  >  SRR19973067.1496262/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                  
CTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTGAA  >  CP009273/4248968‑4249263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑