Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,249,117 G→T 100% intergenic (+68/‑48) dinF → / → yjbJ oxidative stress resistance protein; putative MATE family efflux pump; UV and mitomycin C inducible protein/stress‑induced protein, UPF0337 family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,249,1170GT99.0% 711.5 / ‑2.0 221intergenic (+68/‑48)dinF/yjbJoxidative stress resistance protein; putative MATE family efflux pump; UV and mitomycin C inducible protein/stress‑induced protein, UPF0337 family
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (118/100);  total (119/102)
Fisher's exact test for biased strand distribution p-value = 5.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 221 total aligned reads displayed.

TGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCAT  >  CP009273/4248973‑4249259
                                                                                                                                                |                                                                                                                                              
tGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATaaa                                                                                                                                             <  1:3321473/148‑1 (MQ=255)
tGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATaaa                                                                                                                                             <  1:2629526/148‑1 (MQ=255)
tGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATaaa                                                                                                                                             <  1:1378933/148‑1 (MQ=255)
tGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATaaa                                                                                                                                             <  1:2332206/148‑1 (MQ=255)
tGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATaaa                                                                                                                                             <  2:2364762/148‑1 (MQ=255)
   cGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGt                                                                                                                                          <  2:3008250/148‑1 (MQ=255)
    gTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTg                                                                                                                                         <  2:81738/148‑1 (MQ=255)
      tGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGaa                                                                                                                                       >  2:1591082/1‑148 (MQ=255)
      tGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGaa                                                                                                                                       <  2:2629378/148‑1 (MQ=255)
        cgcgGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACg                                                                                                                                     <  1:2974904/148‑1 (MQ=255)
          cgGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTa                                                                                                                                   >  2:138055/1‑148 (MQ=255)
              cTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTActct                                                                                                                               <  2:1243923/148‑1 (MQ=255)
                   tctGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTc                                                                                                                          <  2:2548969/148‑1 (MQ=255)
                    ctGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTca                                                                                                                         >  1:429249/1‑148 (MQ=255)
                       gcggcTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACaa                                                                                                                      >  1:780442/1‑148 (MQ=255)
                             aTCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAAc                                                                                                                >  2:3191528/1‑148 (MQ=255)
                             aTCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAAc                                                                                                                >  1:2415431/1‑148 (MQ=255)
                              tCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACg                                                                                                               <  2:1182292/148‑1 (MQ=255)
                                    ggcgTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAgg                                                                                                         <  1:2428570/148‑1 (MQ=255)
                                      cgTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTc                                                                                                       >  1:527463/1‑148 (MQ=255)
                                         cACTGGCGCACTGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTg                                                                                                    >  1:745441/1‑148 (MQ=255)
                                         cACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTg                                                                                                    >  2:1602949/1‑148 (MQ=255)
                                         cACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTg                                                                                                    <  2:2703108/148‑1 (MQ=255)
                                           cTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGAt                                                                                                  >  2:652074/1‑148 (MQ=255)
                                            tGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGAtt                                                                                                 <  2:66540/148‑1 (MQ=255)
                                               cgcAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATg                                                                                              >  2:2507100/1‑148 (MQ=255)
                                                   aTGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATa                                                                                          >  2:1878399/1‑148 (MQ=255)
                                                    tGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATaa                                                                                         <  1:1145244/148‑1 (MQ=255)
                                                    tGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATaa                                                                                         <  1:2894549/148‑1 (MQ=255)
                                                      gTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAg                                                                                       <  2:891997/148‑1 (MQ=255)
                                                      gTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAg                                                                                       <  1:2728356/148‑1 (MQ=255)
                                                      gTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAg                                                                                       <  1:2846333/148‑1 (MQ=255)
                                                         ccTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATg                                                                                    <  2:1776613/148‑1 (MQ=255)
                                                         ccTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATg                                                                                    <  2:2244775/148‑1 (MQ=255)
                                                         ccTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATg                                                                                    <  2:3017773/148‑1 (MQ=255)
                                                           tGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGaa                                                                                  >  1:2971151/1‑148 (MQ=255)
                                                            ggTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGCAg                                                                                 >  2:1365490/1‑148 (MQ=255)
                                                            ggTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAg                                                                                 <  2:3140732/148‑1 (MQ=255)
                                                                  ccGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGACGCcggcg                                                                           >  2:2828665/1‑148 (MQ=255)
                                                                     cAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTa                                                                        >  1:1450667/1‑148 (MQ=255)
                                                                     cAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTa                                                                        >  1:2262072/1‑148 (MQ=255)
                                                                      aaCGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTaa                                                                       <  2:776001/148‑1 (MQ=255)
                                                                        cGTGACTGTTAAAAATTCTTAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACt                                                                     <  2:2423074/148‑1 (MQ=255)
                                                                        cGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACt                                                                     >  1:1888023/1‑148 (MQ=255)
                                                                           gACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGa                                                                  >  1:1392629/1‑148 (MQ=255)
                                                                             cGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGaaa                                                                <  2:2927360/148‑1 (MQ=255)
                                                                             cGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCGAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGaaa                                                                <  2:3130078/148‑1 (MQ=255)
                                                                               gTTATAAATTCTGAATAATTAATCCTTATCCAAATTGCTGACTACACTTAATATCACGTTCAGAATAAAAGTGTACGTACTCTAATTCACATCCTAACTATGATGTCTTGATTATGAATAAAGATGTAGCCTGCGGTAACAGTAAACa                                                              <  2:602588/148‑1 (MQ=255)
                                                                                ttAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAg                                                             >  1:1329259/1‑148 (MQ=255)
                                                                                ttAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAg                                                             <  1:3184098/148‑1 (MQ=255)
                                                                                ttAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAg                                                             >  1:3509885/1‑148 (MQ=255)
                                                                                 tAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGt                                                            <  1:1752673/148‑1 (MQ=255)
                                                                                     aaTTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTaa                                                        >  2:1494573/1‑148 (MQ=255)
                                                                                       ttCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAg                                                      <  1:18564/148‑1 (MQ=255)
                                                                                       ttCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAg                                                      >  2:1779801/1‑148 (MQ=255)
                                                                                       ttCTGAATAAATAATCCTAAGACAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAg                                                      <  2:1610076/148‑1 (MQ=255)
                                                                                        tCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAgg                                                     >  2:1538860/1‑148 (MQ=255)
                                                                                        tCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAgg                                                     <  2:1724235/148‑1 (MQ=255)
                                                                                        tCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAgg                                                     >  1:1606643/1‑148 (MQ=255)
                                                                                        tCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAgg                                                     >  1:2710436/1‑148 (MQ=255)
                                                                                           gAATACATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTaa                                                  <  2:2354240/148‑1 (MQ=255)
                                                                                           gAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTaa                                                  >  2:1836731/1‑148 (MQ=255)
                                                                                            aataaataATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGACGCCGGCGGTAACTGGAAACAGTTTAAAGGTaaa                                                 >  2:1830003/1‑148 (MQ=255)
                                                                                              taaataaTCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGt                                               >  1:930015/1‑148 (MQ=255)
                                                                                              taaataaTCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGt                                               >  1:930096/1‑148 (MQ=255)
                                                                                              taaataaTCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGt                                               >  1:1082076/1‑148 (MQ=255)
                                                                                              taaataaTCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGt                                               >  1:2683543/1‑148 (MQ=255)
                                                                                                aataatCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGa                                             >  1:3232676/1‑148 (MQ=255)
                                                                                                aataatCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGa                                             >  1:1247400/1‑148 (MQ=255)
                                                                                                 ataatCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGaa                                            >  1:998282/1‑148 (MQ=255)
                                                                                                 ataatCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGaa                                            <  1:1394682/148‑1 (MQ=255)
                                                                                                   aatCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAag                                          >  1:3108248/1‑148 (MQ=255)
                                                                                                     tCCTAAGCCAAATTGCTGACTACACTTAATCTCACTTTCATAATAAAAGTGAACTTACTCTCATTCACAACCTAACGATGAGGTCTTTATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAagag                                        <  2:2156403/148‑1 (MQ=255)
                                                                                                     tCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAagag                                        <  2:2391846/148‑1 (MQ=255)
                                                                                                        tAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCaa                                     >  2:1681726/1‑148 (MQ=255)
                                                                                                         aaGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGACGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAAt                                    >  2:344806/1‑148 (MQ=255)
                                                                                                           gCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATgg                                  >  1:769788/1‑148 (MQ=255)
                                                                                                            ccAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATggg                                 >  2:2169529/1‑148 (MQ=255)
                                                                                                             cAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGACGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATgggg                                >  2:1368444/1‑148 (MQ=255)
                                                                                                             cAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATgggg                                <  1:2557830/148‑1 (MQ=255)
                                                                                                              aaaTTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGc                               >  2:444248/1‑148 (MQ=255)
                                                                                                                  tGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAAc                           >  2:3104214/1‑148 (MQ=255)
                                                                                                                   gCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACt                          <  2:2415450/148‑1 (MQ=255)
                                                                                                                    cTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTg                         >  2:505868/1‑148 (MQ=255)
                                                                                                                      gACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGAc                       >  1:2067789/1‑148 (MQ=255)
                                                                                                                         tACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCga                    <  2:745441/148‑1 (MQ=255)
                                                                                                                            acTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAACGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCgatga                 <  2:3161670/148‑1 (MQ=255)
                                                                                                                             cTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCgatgat                >  2:184380/1‑148 (MQ=255)
                                                                                                                             cTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCgatgat                <  2:695614/148‑1 (MQ=255)
                                                                                                                             cTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTAATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCgatgat                >  2:388150/1‑148 (MQ=255)
                                                                                                                             cTTAATCTCAAGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCgatgat                <  2:3263513/148‑1 (MQ=255)
                                                                                                                              ttAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCgatgatg               <  2:2614736/148‑1 (MQ=255)
                                                                                                                               tAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCAGGCGGTAACTGGAAACAGTTTAAAGGTAAAGAGAAAGAGCAATGGGGCAAACTGACCgatgatga              >  1:634059/1‑148 (MQ=255)
                                                                                                                                 aTCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGata            >  2:2204479/1‑148 (MQ=255)
                                                                                                                                  tctcACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGatat           >  2:1117903/1‑148 (MQ=255)
                                                                                                                                   ctcACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATg          >  1:1935157/1‑148 (MQ=255)
                                                                                                                                       cGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGa      >  2:213841/1‑148 (MQ=255)
                                                                                                                                          tCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGACGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCa   >  1:1510593/1‑148 (MQ=255)
                                                                                                                                           cAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCAt  >  2:36864/1‑148 (MQ=255)
                                                                                                                                           cAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCAt  >  2:2088787/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                              
TGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCAT  >  CP009273/4248973‑4249259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTGAAG  >  CP009273/4248968‑4249264
                                                                                                                                                     |                                                                                                                                                   
CTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAT                                                                                                                                                     >  SRR19973065.1508220/1‑150 (MQ=60)
   TCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAA                                                                                                                                                  <  SRR19973065.1127477/150‑1 (MQ=60)
     TGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAG                                                                                                                                                <  SRR19973065.2645788/150‑1 (MQ=60)
     TGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAG                                                                                                                                                <  SRR19973065.2346785/150‑1 (MQ=60)
     TGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAG                                                                                                                                                <  SRR19973065.2930409/150‑1 (MQ=60)
     TGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAG                                                                                                                                                <  SRR19973065.3341735/150‑1 (MQ=60)
     TGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAG                                                                                                                                                <  SRR19973065.1388019/150‑1 (MQ=60)
             CGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTA                                                                                                                                        <  SRR19973065.2993097/150‑1 (MQ=60)
                GGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTC                                                                                                                                     <  SRR19973065.2731032/150‑1 (MQ=60)
                GGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTC                                                                                                                                     >  SRR19973065.656058/1‑150 (MQ=60)
                   CTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCA                                                                                                                                  >  SRR19973065.3107722/1‑150 (MQ=60)
                       CTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCA                                                                                                                              >  SRR19973065.432230/1‑150 (MQ=60)
                          TGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAA                                                                                                                           >  SRR19973065.785624/1‑150 (MQ=60)
                          TGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAA                                                                                                                           >  SRR19973065.1266621/1‑150 (MQ=60)
                                CTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAAC                                                                                                                     >  SRR19973065.1189777/1‑150 (MQ=60)
                                CTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAAC                                                                                                                     >  SRR19973065.2430647/1‑150 (MQ=60)
                                CTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAAC                                                                                                                     >  SRR19973065.2430666/1‑150 (MQ=60)
                                 TATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACG                                                                                                                    >  SRR19973065.2677484/1‑150 (MQ=60)
                                 TATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACG                                                                                                                    <  SRR19973065.2205567/150‑1 (MQ=60)
                                   TCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGAT                                                                                                                  <  SRR19973065.2598492/150‑1 (MQ=60)
                                    CTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATG                                                                                                                 >  SRR19973065.381077/1‑150 (MQ=60)
                                     TGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGA                                                                                                                >  SRR19973065.271986/1‑150 (MQ=60)
                                      GGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAG                                                                                                               <  SRR19973065.3141559/150‑1 (MQ=60)
                                         GGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTC                                                                                                            <  SRR19973065.2443897/150‑1 (MQ=60)
                                         GGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTC                                                                                                            >  SRR19973065.525609/1‑150 (MQ=60)
                                         GGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTC                                                                                                            >  SRR19973065.530922/1‑150 (MQ=60)
                                            GTCACTGGCGCACTGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTG                                                                                                         >  SRR19973065.750429/1‑150 (MQ=60)
                                                         TGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAG                                                                                            <  SRR19973065.2912225/150‑1 (MQ=60)
                                                         TGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAG                                                                                            <  SRR19973065.1152633/150‑1 (MQ=60)
                                                         TGGGACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCATACGCCAAATTGCTGACTACACTTAATCTCACGTTAAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGTATAAAG                                                                                            <  SRR19973065.2912160/150‑1 (MQ=60)
                                                           GTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGAT                                                                                          <  SRR19973065.2863846/150‑1 (MQ=60)
                                                           GTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGAT                                                                                          <  SRR19973065.2746777/150‑1 (MQ=60)
                                                           GTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGAT                                                                                          <  SRR19973065.2745400/150‑1 (MQ=60)
                                                           GTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGAT                                                                                          <  SRR19973065.2745325/150‑1 (MQ=60)
                                                              CCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAA                                                                                       >  SRR19973065.2989381/1‑150 (MQ=60)
                                                              CCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAA                                                                                       >  SRR19973065.2989323/1‑150 (MQ=60)
                                                                TGGTTTGCCGCATCGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGC                                                                                     <  SRR19973065.343987/150‑1 (MQ=60)
                                                                  GTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCG                                                                                   >  SRR19973065.1469410/1‑150 (MQ=60)
                                                                  GTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCG                                                                                   <  SRR19973065.173652/150‑1 (MQ=60)
                                                                        CGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTA                                                                             >  SRR19973065.2276374/1‑150 (MQ=60)
                                                                        CGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTA                                                                             >  SRR19973065.1460028/1‑150 (MQ=60)
                                                                           AACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACT                                                                          >  SRR19973065.1900441/1‑150 (MQ=60)
                                                                           AACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACT                                                                          <  SRR19973065.3200312/150‑1 (MQ=60)
                                                                             CGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGATGTCTTGATTATGAATAAAGATGAATCAGGCGGTAACTGG                                                                        >  SRR19973065.1868831/1‑150 (MQ=60)
                                                                              GTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGA                                                                       >  SRR19973065.1401771/1‑150 (MQ=60)
                                                                              GTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGA                                                                       >  SRR19973065.1401347/1‑150 (MQ=60)
                                                                                  CGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACA                                                                   >  SRR19973065.1846560/1‑150 (MQ=60)
                                                                                   GGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAG                                                                  >  SRR19973065.1338052/1‑150 (MQ=60)
                                                                                   GGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAG                                                                  >  SRR19973065.3531178/1‑150 (MQ=60)
                                                                                     TTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTT                                                                <  SRR19973065.3203327/150‑1 (MQ=60)
                                                                                      TAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTT                                                               <  SRR19973065.1764210/150‑1 (MQ=60)
                                                                                        AAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAA                                                             >  SRR19973065.1978840/1‑150 (MQ=60)
                                                                                          AATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGACGCCGGCGGTAACTGGAAACAGTTTAAAG                                                           >  SRR19973065.2186437/1‑150 (MQ=60)
                                                                                          AATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAG                                                           >  SRR19973065.995882/1‑150 (MQ=60)
                                                                                           ATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGG                                                          >  SRR19973065.2727289/1‑150 (MQ=60)
                                                                                           ATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGG                                                          >  SRR19973065.1617398/1‑150 (MQ=60)
                                                                                            TTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGT                                                         <  SRR19973065.18822/150‑1 (MQ=60)
                                                                                              CTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAA                                                       >  SRR19973065.773922/1‑150 (MQ=60)
                                                                                               TGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAA                                                      >  SRR19973065.2551880/1‑150 (MQ=60)
                                                                                                 AATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGACGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGT                                                    >  SRR19973065.1089219/1‑150 (MQ=60)
                                                                                                 AATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGT                                                    >  SRR19973065.2700185/1‑150 (MQ=60)
                                                                                                 AATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGT                                                    >  SRR19973065.936216/1‑150 (MQ=60)
                                                                                                 AATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGT                                                    >  SRR19973065.936158/1‑150 (MQ=60)
                                                                                                 AATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGT                                                    >  SRR19973065.936135/1‑150 (MQ=60)
                                                                                                   TAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGA                                                  >  SRR19973065.1255615/1‑150 (MQ=60)
                                                                                                   TAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGA                                                  >  SRR19973065.3252110/1‑150 (MQ=60)
                                                                                                   TAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTGGATTATGAATAAAGATGACGCCGGCGGTAACTGGACACAGTTTAAAGGTAAAGTGA                                                  >  SRR19973065.3255271/1‑150 (MQ=60)
                                                                                                    AAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAA                                                 >  SRR19973065.1004914/1‑150 (MQ=60)
                                                                                                    AAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAA                                                 >  SRR19973065.892126/1‑150 (MQ=60)
                                                                                                      ATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAG                                               >  SRR19973065.3127205/1‑150 (MQ=60)
                                                                                                      ATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAG                                               <  SRR19973065.1403788/150‑1 (MQ=60)
                                                                                                      ATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAG                                               <  SRR19973065.1403838/150‑1 (MQ=60)
                                                                                                       TAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGA                                              <  SRR19973065.1374517/150‑1 (MQ=60)
                                                                                                              AAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGACGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGG                                       >  SRR19973065.774960/1‑150 (MQ=60)
                                                                                                              AAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGG                                       >  SRR19973065.774906/1‑150 (MQ=60)
                                                                                                               AGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAATGGTAAAGTGAAAGAGCAATGGG                                      >  SRR19973065.2339957/1‑150 (MQ=60)
                                                                                                                  CAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCA                                   >  SRR19973065.1574639/1‑150 (MQ=60)
                                                                                                                  CAAATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCA                                   <  SRR19973065.2573824/150‑1 (MQ=60)
                                                                                                                    AATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAA                                 <  SRR19973065.2372893/150‑1 (MQ=60)
                                                                                                                     ATTGCTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAAC                                <  SRR19973065.139240/150‑1 (MQ=60)
                                                                                                                         CTGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGAC                            >  SRR19973065.2080960/1‑150 (MQ=60)
                                                                                                                          TGACTACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACC                           >  SRR19973065.3232899/1‑150 (MQ=60)
                                                                                                                              TACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATG                       >  SRR19973065.1099578/1‑150 (MQ=60)
                                                                                                                               ACACTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGA                      >  SRR19973065.53419/1‑150 (MQ=60)
                                                                                                                                  CTTAATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCAGGCGGTAACTGGAAACAGTTTAAAGGTAAAGAGAAAGAGCAATGGGGCAAACTGACCGATGATGA                   >  SRR19973065.638192/1‑150 (MQ=60)
                                                                                                                                     AATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATAT                <  SRR19973065.83303/150‑1 (MQ=60)
                                                                                                                                      ATCTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATG               >  SRR19973065.1947824/1‑150 (MQ=60)
                                                                                                                                        CTCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGAC             <  SRR19973065.1633725/150‑1 (MQ=60)
                                                                                                                                         TCACGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACG            >  SRR19973065.1414416/1‑150 (MQ=60)
                                                                                                                                            CGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATC         <  SRR19973065.2643675/150‑1 (MQ=60)
                                                                                                                                            CGTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATC         <  SRR19973065.1946671/150‑1 (MQ=60)
                                                                                                                                             GTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGACGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCA        >  SRR19973065.1520425/1‑150 (MQ=60)
                                                                                                                                             GTTCAGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCA        >  SRR19973065.495978/1‑150 (MQ=60)
                                                                                                                                                 AGAATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTGA    >  SRR19973065.1044164/1‑150 (MQ=60)
                                                                                                                                                   AATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGACGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTGAAG  >  SRR19973065.622246/1‑150 (MQ=60)
                                                                                                                                                   AATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTGAAG  >  SRR19973065.622065/1‑150 (MQ=60)
                                                                                                                                                   AATAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTGAAG  >  SRR19973065.2635641/1‑150 (MQ=60)
                                                                                                                                                   AATAAAAGTGAACGTACTCACATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTGAAG  <  SRR19973065.2919340/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
CTTTCTGGCGTTGCGCGGGCTTTCTCTGGCGGCTATCTGGCGGCGTCACTGGCGCAATGGTACCTGGTTTGCCGCAACGTGACGGTTAAAAATTCTGAATAAATAATCCTAAGCCAAATTGCTGACTACACTTAATCTCACGTTCAGAAGAAAAGTGAACGTACTCTCATTCACAACCTAACGATGAGGTCTTGATTATGAATAAAGATGAAGCCGGCGGTAACTGGAAACAGTTTAAAGGTAAAGTGAAAGAGCAATGGGGCAAACTGACCGATGATGATATGACGATCATTGAAG  >  CP009273/4248968‑4249264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑