Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,118,591 C→A 50.2% G47C (GGT→TGT)  yceB ← lipoprotein, DUF1439 family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,118,5910CA50.2% 76.3 / 311.7 183G47C (GGT→TGT) yceBlipoprotein, DUF1439 family
Reads supporting (aligned to +/- strand):  ref base C (45/46);  new base A (37/55);  total (82/101)
Fisher's exact test for biased strand distribution p-value = 2.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.49e-01

Only 100 of 185 total aligned reads displayed.

TTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCC  >  CP009273/1118445‑1118737
                                                                                                                                                   |                                                                                                                                                  
tttCATGGTCGCTTTCTGACTAACGAACAGGGAGTTCATGTCCAGATTTGCGTCTCCGGTTAGGGTAACCTTATTAGGCTCATCGCGCCCAATTT‑TGCTGGTCAGGTTTGTCAGAACAATAAGGGCGTCAGCCACGCCCGGTAAACCg                                                                                                                                                   >  2:673916/1‑148 (MQ=255)
 ttCATGGTCGCTTTCGGACTACCGAACAGGGAGTTCATGTCCAGATGGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGa                                                                                                                                                  <  2:2546197/148‑1 (MQ=255)
  tCATGGTCGCTTTCTGACTCCCGACCAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTCGGGTAACCTTAGTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACAGat                                                                                                                                                 <  2:499897/148‑1 (MQ=255)
    aTGGTCGCTTTCTGACTACCGACCAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGatat                                                                                                                                               <  2:2377805/148‑1 (MQ=255)
        tCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCttt                                                                                                                                           <  2:2536466/148‑1 (MQ=255)
         cGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATGGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCtttt                                                                                                                                          <  2:431259/148‑1 (MQ=255)
           cTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTga                                                                                                                                        >  2:2653280/1‑148 (MQ=255)
           cTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTga                                                                                                                                        >  2:867290/1‑148 (MQ=255)
           cTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTga                                                                                                                                        >  2:2236962/1‑148 (MQ=255)
            tttCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGCCACCCACGCCGGGTAAACCGATATCTTTTgag                                                                                                                                       <  2:1241060/148‑1 (MQ=255)
             ttCTTAATACCTAACTGGGTGTTATTGTCCAGTTTGGCGTGTCGGTTTTGGGTAACATTATTCGGCTCTGCGCGACAATTTG‑GGGTGGTCTGTTTTGAAACAACAATTTGGGCGACACCCACGCCGGGTAAACCGATATCTTTTgaga                                                                                                                                      <  1:2797408/148‑1 (MQ=255)
             ttCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTgaga                                                                                                                                      <  2:2200539/148‑1 (MQ=255)
              tCTGACTACCGAACAGGGAGTTCATGTCCAGATGGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGaa                                                                                                                                     <  2:1721921/148‑1 (MQ=255)
              tCTGACTACCGAACAGGGAGTTCAGGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGaa                                                                                                                                     <  2:1128081/148‑1 (MQ=255)
               cTGACTACCGAACCGGGAGTTCATGTCCAGATTGGCGTCTCCGTTTTGGTTAACATTATTCGGCTCTTCGCCACCATTTT‑GGGTGGTCTGGTTTGTCAGAACAATATGGGCGACAGCCACGCCGGGTAAACCGATATCTTTTGAGaaa                                                                                                                                    <  1:2601081/148‑1 (MQ=255)
                  aCTACCGAACAGGGTGTTCATGTCCAGTTTGGCGTCTCCTGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAatta                                                                                                                                 <  2:2939638/148‑1 (MQ=255)
                  aCTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAatta                                                                                                                                 <  2:2939853/148‑1 (MQ=255)
                   cTACCGAACTGGGAGTTCATGTCCATTTTGGCGTCTCCGGTTTGGGTAACCTTATTCGGCTCTTCGCGACAAATTT‑GGGTGGTATGTTTTGTCACAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAattat                                                                                                                                <  1:2881920/148‑1 (MQ=255)
                    tACCGAACTGGGAGTTCATGTCCAGTTGGGCGTCTCGGTTTTGGTTAACCTTATTCGGCTCTTCGCGACCATTTT‑GGGTGGTCTGGTTTGTCACAAAAATTTGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAattatt                                                                                                                               <  1:2238392/148‑1 (MQ=255)
                      ccGAACTGGTTGTTCTTGTCCTGATTGGGGTCTCGGGTTTGGGTAACCTTTTTCGGCTCTTCGCGACCAATTG‑GGCTGGTCAGGTTTTTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAATtattat                                                                                                                             <  1:2219279/148‑1 (MQ=255)
                       cGAACTGGTAGTTCTTGGCCATATTGGCGTCTCCGGTTTGGGTAACGTTATGCGGCTCTTCGCGACCAATTT‑GGGTGGTCTGTTTTGTCATAAAAATATGGGCGTCACCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATg                                                                                                                            <  1:2776492/148‑1 (MQ=255)
                       cGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATAGCTTTTGAGAAATTATTATg                                                                                                                            >  2:2845821/1‑148 (MQ=255)
                        gAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGt                                                                                                                           <  2:1693047/148‑1 (MQ=255)
                         aaCAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGtt                                                                                                                          >  1:1576678/1‑148 (MQ=255)
                          aCAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGttt                                                                                                                         >  1:2820390/1‑148 (MQ=255)
                             gggAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGTTTCGc                                                                                                                      >  2:2004417/1‑148 (MQ=255)
                                aGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGTTTCGCAAg                                                                                                                   <  2:2238017/148‑1 (MQ=255)
                                 gTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGc                                                                                                                  >  2:1789973/1‑148 (MQ=255)
                                 gTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGc                                                                                                                  >  2:136477/1‑148 (MQ=255)
                                   tCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGa                                                                                                                >  2:143943/1‑148 (MQ=255)
                                    cATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGAc                                                                                                               <  2:2903284/148‑1 (MQ=255)
                                       gTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCTCCGCCGGGTAAACCGATATCTTTTGAGAAATTAGTATGTTTCGCAAGCGACTgg                                                                                                            >  1:3296094/1‑148 (MQ=255)
                                       gTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTgg                                                                                                            >  1:1624791/1‑148 (MQ=255)
                                       gTCCAGATTGGCGTCGCGGGTTAGGTTAACCTTATTCGGCTCTTCGCGACAATTTT‑GGCTGGTATGTTTTGTCATAACATTTGGGGCTTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTgg                                                                                                            <  2:1040961/148‑1 (MQ=255)
                                         cctttttGGCTTCTCGGTTTTGGTTAACTTTTTTGGGATCTTCGCGACAAATTT‑GGGTGTTCTGTTTTTTCATAAAATTTTGGGGTTCAGCCACGCCGGGTAAACCGATATCTTTTGATAAATTATTATGTTTCGCAAGCGACTGGtt                                                                                                          <  1:2311026/143‑1 (MQ=255)
                                           aGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCATTTT‑GGCTGGTCAGGTTTGTCAGAACAATAGGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTaa                                                                                                        <  2:576530/148‑1 (MQ=255)
                                            taTTGGGGTCTCCGGTTAGGTTAACCTTATTCGGCTCTTCGCCACAAATTT‑GGCTGGTCTGTTTTGTAATAAAAATTTGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAAt                                                                                                       <  2:1581213/147‑1 (MQ=255)
                                             aTTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAAtt                                                                                                      >  1:1692982/1‑148 (MQ=255)
                                                 gcgcCTCGGTTTTGGGTAACCTTATTCGCCTCTCCGCCACAATTTG‑GGCTGGTCTGTTTTTTCAAAACATTTGGGGCGTCCCCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCtt                                                                                                  <  1:2239587/148‑1 (MQ=255)
                                                 gCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCtt                                                                                                  >  1:693532/1‑148 (MQ=255)
                                                 gCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCtt                                                                                                  >  1:892491/1‑148 (MQ=255)
                                                  cGTCTCCGTTTAGGTTAACATTTTTCGGATCTGCGCCACAATTTG‑GGGTGGTCTGTTTTGTAATAACATTATGGGCTACAGCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTg                                                                                                 <  2:23348/148‑1 (MQ=255)
                                                     ctcCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTc                                                                                              >  1:1032475/1‑148 (MQ=255)
                                                     ctcCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTc                                                                                              >  1:955933/1‑148 (MQ=255)
                                                      tcCGGTTTGGGTAACATTATGCGGCTCTTCGCGACCAATTT‑GGCTGGTCTGTTTTGTCACAACAATATGGGCGTCCGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCg                                                                                             <  1:279305/148‑1 (MQ=255)
                                                      tcCGGTTAGGGTAACCTTATCCGGCTCTTCGCGACCATTTT‑GGCGGGTCAGGTTTGTCATAACAATTTGGGCGACAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCg                                                                                             <  2:893321/148‑1 (MQ=255)
                                                       ccGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCgg                                                                                            >  2:2563090/1‑148 (MQ=255)
                                                           ttAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGAt                                                                                        >  1:3117055/1‑148 (MQ=255)
                                                            tAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATg                                                                                       >  1:2233992/1‑148 (MQ=255)
                                                              gggTAACCTTATTCGGCTCTTCGCGACCATTTT‑GGCTGGTCCGTTTTGTAATAACAATCGGGGCGTCACCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGgt                                                                                     <  2:357732/148‑1 (MQ=255)
                                                                 tAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTa                                                                                  >  1:1916714/1‑148 (MQ=255)
                                                                    ccTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTg                                                                               >  1:702575/1‑148 (MQ=255)
                                                                      ttATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTggg                                                                             >  2:2989237/1‑148 (MQ=255)
                                                                       tATGCGGCTCTTCGCGACCAATTT‑GGGTGGTCAGGTTTGTCACAAAAATAGGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGt                                                                            <  2:1274563/148‑1 (MQ=255)
                                                                        aTTCGGCTCTTCGCGACCAATTT‑GGCTGGACAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTg                                                                           <  2:1142140/148‑1 (MQ=255)
                                                                          tCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAg                                                                         >  2:831273/1‑148 (MQ=255)
                                                                             gCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTg                                                                      <  2:1916714/148‑1 (MQ=255)
                                                                              ctctCCGCGACAATTTG‑GGGTGTTCTGGTTTTTCAAAACATTCGGGGCGACCGCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGa                                                                     <  1:3391964/148‑1 (MQ=255)
                                                                               tctTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGAt                                                                    >  2:692660/1‑148 (MQ=255)
                                                                                  tCGCTACCTTTTG‑GGGTGGTCAGTTTTGACATAAAATTGGGGGCGACCCCCACGCCGGTTAAACCTATATCTTTTGAGAAATTATTATGTTTCGAAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTAc                                                                 <  1:62783/148‑1 (MQ=255)
                                                                                  tCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTAc                                                                 >  1:2184394/1‑148 (MQ=255)
                                                                                       aCCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCa                                                            >  1:1277725/1‑148 (MQ=255)
                                                                                        acAATTTGGGGTGGTCAGGTTTGACACAAAAATTGGGGCTTCACCCACGCCGGGTAAACAGATATCTTTTGATAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCa                                                            <  1:439959/147‑1 (MQ=255)
                                                                                           aTTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGTTTCGCAAACGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGa                                                        >  2:297460/1‑148 (MQ=255)
                                                                                                 gCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAgg                                                   >  1:808835/1‑148 (MQ=255)
                                                                                                 gCTGGTCAGGTTTGTCAGAACAATAGGGGCGTCCGCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAgg                                                   <  1:2305653/148‑1 (MQ=255)
                                                                                                  cTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGc                                                  <  2:1701404/148‑1 (MQ=255)
                                                                                                  cTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGc                                                  >  1:2188319/1‑148 (MQ=255)
                                                                                                    ggTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCa                                                >  2:2606564/1‑148 (MQ=255)
                                                                                                    ggTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCa                                                >  1:1086656/1‑148 (MQ=255)
                                                                                                    ggTAAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCa                                                <  2:1489871/148‑1 (MQ=255)
                                                                                                     gTCAGTTTTGTCATAACAATTTGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCAc                                               <  2:695199/148‑1 (MQ=255)
                                                                                                       cAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTg                                             >  1:800960/1‑148 (MQ=255)
                                                                                                           tttGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGa                                         >  1:161521/1‑148 (MQ=255)
                                                                                                            ttGACATAACAATAGGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGAt                                        <  1:2975937/148‑1 (MQ=255)
                                                                                                             tGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATc                                       <  2:2568185/148‑1 (MQ=255)
                                                                                                              gTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCa                                      <  2:544431/148‑1 (MQ=255)
                                                                                                              gTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCa                                      <  1:2092811/148‑1 (MQ=255)
                                                                                                              gTCAGAACAAGTTGGGCGCCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCa                                      <  2:1531194/148‑1 (MQ=255)
                                                                                                              gTCAAAACAATTGGGGCGTCACCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCa                                      <  1:347002/148‑1 (MQ=255)
                                                                                                                  gAACAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGc                                  >  1:1985060/1‑148 (MQ=255)
                                                                                                                    aCAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAg                                <  2:3117055/148‑1 (MQ=255)
                                                                                                                    aCAATATGGGCGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAg                                >  2:1942008/1‑148 (MQ=255)
                                                                                                                        tatGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGc                            >  1:99009/1‑148 (MQ=255)
                                                                                                                        tatGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGc                            >  1:2148310/1‑148 (MQ=255)
                                                                                                                           gggCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCaaa                         <  1:1460914/148‑1 (MQ=255)
                                                                                                                           gggCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATGGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCaaa                         >  1:2535998/1‑148 (MQ=255)
                                                                                                                              cGTCAGCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAAATaa                      <  2:2045513/148‑1 (MQ=255)
                                                                                                                                  aGCCACGCCGGGTAAACAGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAAATAAAAAc                  <  2:3312844/148‑1 (MQ=255)
                                                                                                                                     cACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTg               >  1:516860/1‑148 (MQ=255)
                                                                                                                                     cACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTg               >  1:2308732/1‑148 (MQ=255)
                                                                                                                                        gCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTc            <  2:3501533/148‑1 (MQ=255)
                                                                                                                                         ccGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCa           <  2:724786/148‑1 (MQ=255)
                                                                                                                                          cGGTTAAACAGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCAt          <  1:2989237/148‑1 (MQ=255)
                                                                                                                                           gggTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCAGCAAATAAAAACTTGTTCATg         <  1:265882/148‑1 (MQ=255)
                                                                                                                                           gggTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCAGCAAATAAAAACTTGTTCATg         <  1:2836790/148‑1 (MQ=255)
                                                                                                                                           gggTAAACAGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTGCATg         >  2:3050744/1‑148 (MQ=255)
                                                                                                                                            ggTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATgg        >  2:2787464/1‑148 (MQ=255)
                                                                                                                                                aaCCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGt    <  1:1118678/148‑1 (MQ=255)
                                                                                                                                                  ccGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTGATGGTAGTcc  <  1:2378473/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTTCATGGTCGCTTTCTGACTACCGAACAGGGAGTTCATGTCCAGATTGGCGTCTCCGGTTAGGGTAACCTTATTCGGCTCTTCGCGACCAATTT‑GGCTGGTCAGGTTTGTCAGAACAATATGGGCGTCAGCCACGCCGGGTAAACCGATATCTTTTGAGAAATTATTATGTTTCGCAAGCGACTGGTTAATTTCTTGTTCGGTGATGGTGTATTGGGTGAGTTGATTACAGCCAACGAGCAGGCCACTGACGATCAATGCAGCGGCAAATAAAAACTTGTTCATGGTAGTCC  >  CP009273/1118445‑1118737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A