Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,354,405 C→T 92.8% intergenic (+430/‑130) gltD → / → gltF glutamate synthase, 4Fe‑4S protein, small subunit/periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,354,4050CT92.8% 457.7 / 18.9 137intergenic (+430/‑130)gltD/gltFglutamate synthase, 4Fe‑4S protein, small subunit/periplasmic protein
Reads supporting (aligned to +/- strand):  ref base C (5/5);  new base T (76/51);  total (81/56)
Fisher's exact test for biased strand distribution p-value = 7.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 138 total aligned reads displayed.

AAATATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTCATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAA  >  CP009273/3354258‑3354547
                                                                                                                                                   |                                                                                                                                              
aaaTATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTAt                                                                                                                                              <  1:148846/150‑1 (MQ=255)
 aaTATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTAtt                                                                                                                                             <  2:957832/150‑1 (MQ=255)
  atatGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATttatta                                                                                                                                            <  2:1103277/150‑1 (MQ=255)
   tatGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATtaa                                                                                                                                           >  2:1936344/1‑150 (MQ=255)
   tatGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATtaa                                                                                                                                           >  2:1936468/1‑150 (MQ=255)
    atGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATtaaa                                                                                                                                          >  2:1607970/1‑150 (MQ=255)
      gAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATtaaata                                                                                                                                        <  1:778373/150‑1 (MQ=255)
         tataAAAAACCAATATATAATCCATAATTATCTATATATTTTCCTATATTACAGCGCAAACATTCATGTAATGAATAATTATCAAAACAAACAAACTCACCAGCACCTTAATATTCACATGATCGTAACTAAAAAATATataaaataaaa                                                                                                                                     >  2:2866040/1‑149 (MQ=255)
              aaaaCCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATc                                                                                                                                >  2:3274386/1‑150 (MQ=255)
                    aaTATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCa                                                                                                                          >  1:1022530/1‑150 (MQ=255)
                    aaTATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCa                                                                                                                          >  1:3215799/1‑150 (MQ=255)
                          ttatCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTgg                                                                                                                    >  2:882262/1‑150 (MQ=255)
                            atCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCt                                                                                                                  >  1:4075930/1‑150 (MQ=255)
                            atCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCt                                                                                                                  >  1:3306280/1‑150 (MQ=255)
                            atCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTCATTAAATAAATACATCCCAGCAAGTTGGCt                                                                                                                  >  2:317712/1‑150 (MQ=255)
                             tCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCAAGCAAGTTGGCTa                                                                                                                 >  2:3074577/1‑150 (MQ=255)
                             tCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATACCAGCAAGTTGGCTa                                                                                                                 >  2:3545990/1‑150 (MQ=255)
                                  aaTTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTCATTAAATAAATACATCCCAGCAAGTTGGCTATTTCt                                                                                                            <  2:790673/150‑1 (MQ=255)
                                    ttATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGa                                                                                                          <  1:1736488/150‑1 (MQ=255)
                                     tATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGaa                                                                                                         <  2:575150/150‑1 (MQ=255)
                                      aTCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAAc                                                                                                        <  1:2541486/150‑1 (MQ=255)
                                       tCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACg                                                                                                       >  2:865245/1‑150 (MQ=255)
                                         tatataTTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAg                                                                                                     <  2:3663502/150‑1 (MQ=255)
                                         tatataTTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAg                                                                                                     >  1:1203127/1‑150 (MQ=255)
                                         tatataTTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAg                                                                                                     >  1:1203115/1‑150 (MQ=255)
                                          atatatTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGt                                                                                                    <  1:3606206/150‑1 (MQ=255)
                                            atatTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGtta                                                                                                  >  1:4000701/1‑150 (MQ=255)
                                            atatTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGtta                                                                                                  <  2:3646076/150‑1 (MQ=255)
                                              atTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGttatt                                                                                                >  2:3948202/1‑150 (MQ=255)
                                              atTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGttatt                                                                                                >  1:1715759/1‑150 (MQ=255)
                                              atTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCAAATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGttatt                                                                                                >  2:2058401/1‑150 (MQ=255)
                                                tttCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGttattta                                                                                              >  2:3353677/1‑150 (MQ=255)
                                                 ttCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGttatttat                                                                                             >  1:1048270/1‑150 (MQ=255)
                                                    cTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTCATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTAtttattta                                                                                          <  1:3256965/150‑1 (MQ=255)
                                                    cTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTCATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTAtttattta                                                                                          <  1:3257107/150‑1 (MQ=255)
                                                      aTTTTTCAGCGTAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACa                                                                                        <  2:2977005/150‑1 (MQ=255)
                                                      aTTTTTCAGCGTAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACa                                                                                        <  2:2976778/150‑1 (MQ=255)
                                                      aTTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACa                                                                                        <  1:4189941/150‑1 (MQ=255)
                                                       tttttCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACAt                                                                                       <  1:3007702/150‑1 (MQ=255)
                                                        ttttCAGCGCAAACATTCATGTAATGAATAATTATCACCACACACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACAtt                                                                                      <  2:4263815/150‑1 (MQ=255)
                                                        ttttCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACAtt                                                                                      >  1:3805297/1‑150 (MQ=255)
                                                           tCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTcac                                                                                   >  1:1716613/1‑150 (MQ=255)
                                                           tCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTcac                                                                                   >  1:1988441/1‑150 (MQ=255)
                                                            cAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACAACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCAATCAAGTTGGCTATTTCTGAAAGAGTTATTTATTTACATTcaca                                                                                  >  2:3099665/1‑150 (MQ=255)
                                                                gcAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACa                                                                              >  1:622979/1‑150 (MQ=255)
                                                                     cattcattTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGatat                                                                         <  1:3781969/150‑1 (MQ=255)
                                                                     cATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGatat                                                                         <  1:3781645/150‑1 (MQ=255)
                                                                        tCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGatatata                                                                      <  1:761371/150‑1 (MQ=255)
                                                                          aTGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGAtatatata                                                                    <  2:2520699/150‑1 (MQ=255)
                                                                              aaTGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGc                                                                >  1:526015/1‑150 (MQ=255)
                                                                              aaTGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGc                                                                >  1:2700353/1‑150 (MQ=255)
                                                                               aTGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCt                                                               <  2:1022530/150‑1 (MQ=255)
                                                                                 gAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTc                                                             >  2:4098057/1‑150 (MQ=255)
                                                                                  aataatTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCa                                                            <  2:289606/150‑1 (MQ=255)
                                                                                  aataatTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCa                                                            <  2:2521118/150‑1 (MQ=255)
                                                                                   ataatTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAg                                                           <  1:1062322/150‑1 (MQ=255)
                                                                                    taatTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGc                                                          >  1:247430/1‑150 (MQ=255)
                                                                                         aTCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCttttt                                                     <  2:3994325/150‑1 (MQ=255)
                                                                                         aTCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCttttt                                                     >  2:3336032/1‑150 (MQ=255)
                                                                                           caccacAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTttttat                                                   <  2:3564328/150‑1 (MQ=255)
                                                                                           caccacAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTCATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTttttat                                                   >  2:559426/1‑150 (MQ=255)
                                                                                              cacaAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATAATGCTTCAGCTtttaatttt                                                >  2:4274855/1‑150 (MQ=255)
                                                                                              cacaAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTCATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTttttatttt                                                >  2:3421757/1‑150 (MQ=255)
                                                                                                 aaaCCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTaaa                                             >  2:1638299/1‑150 (MQ=255)
                                                                                                 aaaCCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTaaa                                             >  1:220085/1‑150 (MQ=255)
                                                                                                        cTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAaaataaa                                      >  2:1393423/1‑150 (MQ=255)
                                                                                                           accaccAACTTATTATTCACATCATCGAAACTATAAAATTTATTAAATAAATACATCCCACCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAAttt                                   <  2:3715468/150‑1 (MQ=255)
                                                                                                           aCCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAAttt                                   <  2:4143680/150‑1 (MQ=255)
                                                                                                            ccAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTg                                  <  1:1589067/150‑1 (MQ=255)
                                                                                                            ccAGCACCTTATTAGTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTg                                  <  1:1589126/150‑1 (MQ=255)
                                                                                                               gCACCTTATTATTCACATGATCGTAACTATAAAATTTGTTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGtta                               >  2:363463/1‑150 (MQ=255)
                                                                                                                cACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGttat                              >  1:311071/1‑150 (MQ=255)
                                                                                                                cACCTTATTATTCACATGATCGTAACTATAAAATTCATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGttat                              <  2:76141/150‑1 (MQ=255)
                                                                                                                 aCCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGttttt                             >  1:4237436/1‑150 (MQ=255)
                                                                                                                 aCCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGttatt                             >  1:4237652/1‑150 (MQ=255)
                                                                                                                  ccTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGttatta                            <  2:1280313/150‑1 (MQ=255)
                                                                                                                       ttattCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGat                       <  2:3823538/150‑1 (MQ=255)
                                                                                                                       ttattCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGat                       <  2:3785782/150‑1 (MQ=255)
                                                                                                                       ttattCACATGATCGTAACTATAAAACTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGat                       >  2:2508599/1‑150 (MQ=255)
                                                                                                                       ttattCACATGATCGTAACTATAAAACTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGat                       >  2:207430/1‑150 (MQ=255)
                                                                                                                         attCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGatat                     >  1:214559/1‑150 (MQ=255)
                                                                                                                         attCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGatat                     >  1:2414278/1‑150 (MQ=255)
                                                                                                                              caTGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCa                <  2:1545109/150‑1 (MQ=255)
                                                                                                                              caTGATCGTAACTATAAAATTCATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCa                >  2:3192876/1‑150 (MQ=255)
                                                                                                                                tGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCata              <  1:368375/150‑1 (MQ=255)
                                                                                                                                 gATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCatat             >  2:3685915/1‑150 (MQ=255)
                                                                                                                                  aTCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATg            <  2:3306280/150‑1 (MQ=255)
                                                                                                                                    cGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGtt          >  1:3895591/1‑150 (MQ=255)
                                                                                                                                     gTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGTGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTTAGGATATGTTCATATGttt         >  2:1738551/1‑150 (MQ=255)
                                                                                                                                     gTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGttt         >  2:2105708/1‑150 (MQ=255)
                                                                                                                                        aCTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATCTGTTCATATGTTTTTc      >  2:116785/1‑150 (MQ=255)
                                                                                                                                        aCTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTc      >  2:3744071/1‑150 (MQ=255)
                                                                                                                                        aCTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTc      >  1:2148169/1‑150 (MQ=255)
                                                                                                                                        aCTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTc      >  1:4291753/1‑150 (MQ=255)
                                                                                                                                          tataAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCaa    >  1:2037161/1‑150 (MQ=255)
                                                                                                                                          tataAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCaa    >  1:2037755/1‑150 (MQ=255)
                                                                                                                                          tataAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCaa    >  1:3390146/1‑150 (MQ=255)
                                                                                                                                            taAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCaaaa  >  1:1846573/1‑150 (MQ=255)
                                                                                                                                            taAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCaaaa  >  1:1846538/1‑150 (MQ=255)
                                                                                                                                            taAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCaaaa  >  1:2552356/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
AAATATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTCATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAA  >  CP009273/3354258‑3354547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAATATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTCATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACC  >  CP009273/3354258‑3354553
                                                                                                                                                   |                                                                                                                                                    
AAATATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTAT                                                                                                                                                    <  SRR19973079.150011/150‑1 (MQ=60)
   TATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAA                                                                                                                                                 <  SRR19973079.1826501/150‑1 (MQ=60)
    ATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTCATTAAA                                                                                                                                                >  SRR19973079.3925097/1‑150 (MQ=60)
      GAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATA                                                                                                                                              <  SRR19973079.782733/150‑1 (MQ=60)
         TATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAAT                                                                                                                                           >  SRR19973079.2557424/1‑150 (MQ=60)
                    AATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCA                                                                                                                                >  SRR19973079.1028241/1‑150 (MQ=60)
                    AATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCA                                                                                                                                >  SRR19973079.3232166/1‑150 (MQ=60)
                       ATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGT                                                                                                                             >  SRR19973079.4327056/1‑150 (MQ=60)
                          TTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGG                                                                                                                          >  SRR19973079.671056/1‑150 (MQ=60)
                            ATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCT                                                                                                                        >  SRR19973079.4096584/1‑150 (MQ=60)
                            ATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCACAGCAAGTTGGCT                                                                                                                        >  SRR19973079.3323150/1‑150 (MQ=60)
                                    TTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGA                                                                                                                <  SRR19973079.1745700/150‑1 (MQ=60)
                                      ATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAAC                                                                                                              <  SRR19973079.2554298/150‑1 (MQ=60)
                                         TATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAG                                                                                                           >  SRR19973079.1210048/1‑150 (MQ=60)
                                         TATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAG                                                                                                           >  SRR19973079.1210036/1‑150 (MQ=60)
                                          ATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGT                                                                                                          <  SRR19973079.3625036/150‑1 (MQ=60)
                                          ATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGT                                                                                                          <  SRR19973079.3624502/150‑1 (MQ=60)
                                            ATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTA                                                                                                        >  SRR19973079.4020781/1‑150 (MQ=60)
                                              ATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATT                                                                                                      >  SRR19973079.1724918/1‑150 (MQ=60)
                                              ATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATT                                                                                                      >  SRR19973079.1724789/1‑150 (MQ=60)
                                                 TTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTAT                                                                                                   >  SRR19973079.1054052/1‑150 (MQ=60)
                                                    CTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTA                                                                                                >  SRR19973079.1092812/1‑150 (MQ=60)
                                                    CTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTCATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTA                                                                                                <  SRR19973079.3273560/150‑1 (MQ=60)
                                                    CTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTCATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTA                                                                                                <  SRR19973079.3273417/150‑1 (MQ=60)
                                                    CTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTCATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTA                                                                                                <  SRR19973079.3273412/150‑1 (MQ=60)
                                                      ATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACA                                                                                              <  SRR19973079.4211184/150‑1 (MQ=60)
                                                      ATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACA                                                                                              >  SRR19973079.4220053/1‑150 (MQ=60)
                                                       TTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACAT                                                                                             <  SRR19973079.3023234/150‑1 (MQ=60)
                                                        TTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATT                                                                                            >  SRR19973079.3824770/1‑150 (MQ=60)
                                                           TCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCAC                                                                                         >  SRR19973079.1725773/1‑150 (MQ=60)
                                                           TCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCAC                                                                                         >  SRR19973079.1998989/1‑150 (MQ=60)
                                                                GCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACA                                                                                    >  SRR19973079.626574/1‑150 (MQ=60)
                                                                     CATTCATTTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATAT                                                                               <  SRR19973079.3801338/150‑1 (MQ=60)
                                                                     CATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATAT                                                                               <  SRR19973079.3801012/150‑1 (MQ=60)
                                                                      ATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATA                                                                              <  SRR19973079.3279671/150‑1 (MQ=60)
                                                                       TTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATAT                                                                             <  SRR19973079.3817567/150‑1 (MQ=60)
                                                                        TCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATA                                                                            <  SRR19973079.765578/150‑1 (MQ=60)
                                                                            GTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACT                                                                        >  SRR19973079.2762069/1‑150 (MQ=60)
                                                                              AATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGC                                                                      >  SRR19973079.2714334/1‑150 (MQ=60)
                                                                              AATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGC                                                                      >  SRR19973079.529194/1‑150 (MQ=60)
                                                                                   ATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAG                                                                 <  SRR19973079.1068132/150‑1 (MQ=60)
                                                                                    TAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGC                                                                >  SRR19973079.249056/1‑150 (MQ=60)
                                                                                                 AAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAA                                                   >  SRR19973079.1579830/1‑150 (MQ=60)
                                                                                                 AAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAA                                                   >  SRR19973079.221601/1‑150 (MQ=60)
                                                                                                     CACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAAT                                               >  SRR19973079.2015023/1‑150 (MQ=60)
                                                                                                            CCAGCACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTG                                        <  SRR19973079.1597821/150‑1 (MQ=60)
                                                                                                            CCAGCACCTTATTAGTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTG                                        <  SRR19973079.1597880/150‑1 (MQ=60)
                                                                                                                CACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTAT                                    >  SRR19973079.312872/1‑150 (MQ=60)
                                                                                                                 ACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTTTT                                   >  SRR19973079.4258876/1‑150 (MQ=60)
                                                                                                                 ACCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATT                                   >  SRR19973079.4259093/1‑150 (MQ=60)
                                                                                                                  CCTTATTATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATTAATTTGTTATTA                                  >  SRR19973079.2340994/1‑150 (MQ=60)
                                                                                                                         ATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATAT                           >  SRR19973079.216029/1‑150 (MQ=60)
                                                                                                                         ATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATAT                           >  SRR19973079.216043/1‑150 (MQ=60)
                                                                                                                         ATTCACATGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATAT                           >  SRR19973079.2426726/1‑150 (MQ=60)
                                                                                                                                TGATCGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATA                    <  SRR19973079.370360/150‑1 (MQ=60)
                                                                                                                                    CGTAACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTT                >  SRR19973079.3915362/1‑150 (MQ=60)
                                                                                                                                        ACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTC            >  SRR19973079.2159200/1‑150 (MQ=60)
                                                                                                                                        ACTATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTC            >  SRR19973079.4313399/1‑150 (MQ=60)
                                                                                                                                          TATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAA          >  SRR19973079.3407623/1‑150 (MQ=60)
                                                                                                                                          TATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAA          >  SRR19973079.2047872/1‑150 (MQ=60)
                                                                                                                                          TATAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAA          >  SRR19973079.2048468/1‑150 (MQ=60)
                                                                                                                                            TAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAA        >  SRR19973079.1856280/1‑150 (MQ=60)
                                                                                                                                            TAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAA        >  SRR19973079.1856315/1‑150 (MQ=60)
                                                                                                                                            TAAAATTTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAA        >  SRR19973079.2565285/1‑150 (MQ=60)
                                                                                                                                                  TTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACC  >  SRR19973079.4059189/1‑150 (MQ=60)
                                                                                                                                                  TTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACC  <  SRR19973079.1946677/150‑1 (MQ=60)
                                                                                                                                                  TTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACC  <  SRR19973079.1946802/150‑1 (MQ=60)
                                                                                                                                                  TTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACC  >  SRR19973079.536239/1‑150 (MQ=60)
                                                                                                                                                  TTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACC  >  SRR19973079.2187997/1‑150 (MQ=60)
                                                                                                                                                  TTATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACC  >  SRR19973079.2187915/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                    
AAATATGAATATAAAAAACCAATATATTATCCTTAATTATCTATATATTTTCCTATTTTTCAGCGCAAACATTCATGTAATGAATAATTATCACCACAAACCACCTCACCAGCACCTTATTATTCACATGATCGTAACTATAAAATTCATTAAATAAATACATCCCAGCAAGTTGGCTATTTCTGAACGAGTTATTTATTTACATTCACAAACAGATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACC  >  CP009273/3354258‑3354553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑