Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,318,829 G→T 77.1% E159* (GAA→TAA)  yciO → putative RNA binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,318,8290GT77.1% 416.6 / 180.5 262E159* (GAA→TAA) yciOputative RNA binding protein
Reads supporting (aligned to +/- strand):  ref base G (39/21);  new base T (103/99);  total (142/120)
Fisher's exact test for biased strand distribution p-value = 7.61e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01

Only 100 of 266 total aligned reads displayed.

CGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAA  >  CP009273/1318682‑1318975
                                                                                                                                                   |                                                                                                                                                  
cGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGgaataa                                                                                                                                                  >  1:4259886/1‑150 (MQ=255)
 gTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGaaa                                                                                                                                                 <  1:4030787/150‑1 (MQ=255)
  tCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAAt                                                                                                                                                <  1:1708371/150‑1 (MQ=255)
   cTGTTGCAGGAAAAACTCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAAtt                                                                                                                                               >  1:2019100/1‑150 (MQ=255)
   cTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAAtt                                                                                                                                               >  2:793610/1‑150 (MQ=255)
   cTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAAtt                                                                                                                                               <  2:2794768/150‑1 (MQ=255)
      ttGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTaaa                                                                                                                                            <  1:3611302/150‑1 (MQ=255)
      ttGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTaaa                                                                                                                                            >  2:3310963/1‑150 (MQ=255)
      ttGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTaaa                                                                                                                                            >  2:3831246/1‑150 (MQ=255)
       tGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAg                                                                                                                                           <  2:4592348/150‑1 (MQ=255)
         cAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGAt                                                                                                                                         >  2:3530906/1‑150 (MQ=255)
             aaaaaCGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTc                                                                                                                                     >  1:316709/1‑150 (MQ=255)
              aaaaCGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGCATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCt                                                                                                                                    <  1:3813289/150‑1 (MQ=255)
              aaaaCGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATAAACGATCGTCt                                                                                                                                    >  1:551894/1‑150 (MQ=255)
                 aCGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGa                                                                                                                                 >  1:2438671/1‑150 (MQ=255)
                   gCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGaaa                                                                                                                               <  1:892280/150‑1 (MQ=255)
                   gCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGaaa                                                                                                                               <  1:2949271/150‑1 (MQ=255)
                    cAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGaaaa                                                                                                                              >  1:2843845/1‑150 (MQ=255)
                    cAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGaaaa                                                                                                                              <  1:1923028/150‑1 (MQ=255)
                    cAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGaaaa                                                                                                                              >  2:3769025/1‑150 (MQ=255)
                     aaaaCCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGaaaaa                                                                                                                             >  2:1618966/1‑150 (MQ=255)
                       aaCCATCGGTATGCGTGTGCCTTTTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACa                                                                                                                           <  1:4124237/150‑1 (MQ=255)
                          cATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGt                                                                                                                        >  2:885529/1‑150 (MQ=255)
                          cATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGt                                                                                                                        <  1:123589/150‑1 (MQ=255)
                          cATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGt                                                                                                                        >  2:2101137/1‑150 (MQ=255)
                           aTCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTa                                                                                                                       <  2:4168447/150‑1 (MQ=255)
                           aTCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACACGGAATAAATTAAAGATAGTCTGGAAAAAAAGGTa                                                                                                                       >  2:4441207/1‑150 (MQ=255)
                            tCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAg                                                                                                                      >  2:2259917/1‑150 (MQ=255)
                             cGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGa                                                                                                                     >  1:3030917/1‑150 (MQ=255)
                               gTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGAtt                                                                                                                   >  1:1187656/1‑150 (MQ=255)
                               gTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGAtt                                                                                                                   >  1:772296/1‑150 (MQ=255)
                                   gCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGat                                                                                                               >  2:3205869/1‑150 (MQ=255)
                                    cGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGagt                                                                                                              >  1:4784423/1‑148 (MQ=255)
                                      tgtgCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGattat                                                                                                            <  1:3690122/150‑1 (MQ=255)
                                         gCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCa                                                                                                         >  2:3413368/1‑150 (MQ=255)
                                           cTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCAcg                                                                                                       <  1:1674418/150‑1 (MQ=255)
                                                aaCCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATGTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACggcggc                                                                                                  >  2:2404589/1‑150 (MQ=255)
                                                  cccTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTa                                                                                                >  1:2944379/1‑150 (MQ=255)
                                                      aTCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTAtctc                                                                                            <  2:4505224/150‑1 (MQ=255)
                                                        cGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCgg                                                                                          <  2:4085294/150‑1 (MQ=255)
                                                         gCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCggg                                                                                         >  2:4082717/1‑150 (MQ=255)
                                                           tCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCa                                                                                       <  2:3376655/150‑1 (MQ=255)
                                                                cGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAAc                                                                                  <  1:4338939/150‑1 (MQ=255)
                                                                  ttACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCg                                                                                >  1:3415343/1‑150 (MQ=255)
                                                                  ttACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCg                                                                                >  1:3085059/1‑150 (MQ=255)
                                                                   tACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGa                                                                               >  2:1533123/1‑150 (MQ=255)
                                                                   tACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGACTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGa                                                                               <  2:4112682/150‑1 (MQ=255)
                                                                    aCTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGAc                                                                              >  1:327809/1‑150 (MQ=255)
                                                                       tGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAAc                                                                           >  2:4251533/1‑150 (MQ=255)
                                                                        gAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACg                                                                          >  1:2507665/1‑150 (MQ=255)
                                                                          aGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGt                                                                        <  1:1005395/150‑1 (MQ=255)
                                                                          aGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGt                                                                        >  1:4358657/1‑150 (MQ=255)
                                                                           gCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGtt                                                                       <  1:4942108/150‑1 (MQ=255)
                                                                             aTTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTAt                                                                     >  2:1448904/1‑150 (MQ=255)
                                                                               tGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTg                                                                   >  2:4337646/1‑150 (MQ=255)
                                                                               tGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTg                                                                   <  2:3571857/150‑1 (MQ=255)
                                                                                   cGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCt                                                               <  2:2760977/150‑1 (MQ=255)
                                                                                    gAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCtt                                                              >  1:2025511/1‑150 (MQ=255)
                                                                                    gAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTGATTGATCtt                                                              >  2:2365946/1‑150 (MQ=255)
                                                                                      aCCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTAc                                                            >  1:4299275/1‑150 (MQ=255)
                                                                                         gATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCga                                                         >  1:1197572/1‑150 (MQ=255)
                                                                                             cTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCgatgat                                                     >  2:1321849/1‑150 (MQ=255)
                                                                                              tttCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATa                                                    <  1:1791559/150‑1 (MQ=255)
                                                                                              tttCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATa                                                    <  2:3247080/150‑1 (MQ=255)
                                                                                               ttCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATAc                                                   <  1:3205869/150‑1 (MQ=255)
                                                                                                   aCTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCg                                               <  1:3549270/150‑1 (MQ=255)
                                                                                                      tCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTc                                            >  2:2571955/1‑150 (MQ=255)
                                                                                                      tCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTc                                            <  2:4621029/150‑1 (MQ=255)
                                                                                                      tCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTc                                            <  2:737046/150‑1 (MQ=255)
                                                                                                       ctcTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCg                                           >  1:4393249/3‑150 (MQ=255)
                                                                                                           aaTGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCgtggt                                       <  2:3816284/150‑1 (MQ=255)
                                                                                                              gctgcCCGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCg                                    <  1:2671588/150‑1 (MQ=255)
                                                                                                               ctgcCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGt                                   >  2:1694255/1‑150 (MQ=255)
                                                                                                                 gcCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGa                                 <  1:4547403/150‑1 (MQ=255)
                                                                                                                       cAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGt                           >  1:897820/1‑150 (MQ=255)
                                                                                                                       cAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGt                           <  2:2019105/150‑1 (MQ=255)
                                                                                                                       cAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGt                           >  2:1280116/1‑150 (MQ=255)
                                                                                                                        aGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTa                          >  2:1210061/1‑150 (MQ=255)
                                                                                                                           gAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGt                       >  1:4210356/1‑150 (MQ=255)
                                                                                                                            aaTTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTg                      >  2:3870413/1‑150 (MQ=255)
                                                                                                                            aaTTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTg                      <  1:4071938/150‑1 (MQ=255)
                                                                                                                              tttACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGAt                    <  1:2699940/150‑1 (MQ=255)
                                                                                                                              tttACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGAt                    <  2:3052636/150‑1 (MQ=255)
                                                                                                                               ttACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGAtg                   <  2:4755372/150‑1 (MQ=255)
                                                                                                                               ttACCGAATCGGACCCGGAATAAATTAAAGATCGTCGGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGAtg                   <  1:1361046/150‑1 (MQ=255)
                                                                                                                                tACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGAtgt                  <  2:4305820/150‑1 (MQ=255)
                                                                                                                                 aCCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGAtgtg                 >  2:1876386/1‑150 (MQ=255)
                                                                                                                                 aCCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGAtgtg                 <  2:1109460/150‑1 (MQ=255)
                                                                                                                                  ccGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGa                <  1:896965/150‑1 (MQ=255)
                                                                                                                                   cgcaTCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGaa               <  2:4512843/147‑1 (MQ=255)
                                                                                                                                    gAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAg              <  1:4626919/150‑1 (MQ=255)
                                                                                                                                    gAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAg              >  1:140004/1‑150 (MQ=255)
                                                                                                                                    gAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAg              >  2:3597237/1‑150 (MQ=255)
                                                                                                                                      aTCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGcc            >  2:1825342/1‑150 (MQ=255)
                                                                                                                                      aTCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGcc            <  1:1505670/150‑1 (MQ=255)
                                                                                                                                       tCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCt           <  2:40646/150‑1 (MQ=255)
                                                                                                                                        cGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCtt          <  2:4905030/150‑1 (MQ=255)
                                                                                                                                         ggACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCttt         <  1:2252675/150‑1 (MQ=255)
                                                                                                                                          gACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTc        <  1:1027982/150‑1 (MQ=255)
                                                                                                                                                gaataaATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATaa  <  2:1766182/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAA  >  CP009273/1318682‑1318975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAA  >  CP009273/1318681‑1318978
                                                                                                                                                    |                                                                                                                                                     
CCGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATA                                                                                                                                                      >  SRR19973063.106333/1‑150 (MQ=60)
 CGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAA                                                                                                                                                     >  SRR19973063.4273444/1‑150 (MQ=60)
 CGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAA                                                                                                                                                     >  SRR19973063.4273400/1‑150 (MQ=60)
  GTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAA                                                                                                                                                    <  SRR19973063.4043707/150‑1 (MQ=60)
   TCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAAT                                                                                                                                                   <  SRR19973063.1713574/150‑1 (MQ=60)
    CTGTTGCAGGAAAAACTCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATT                                                                                                                                                  >  SRR19973063.2025401/1‑150 (MQ=60)
    CTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATT                                                                                                                                                  >  SRR19973063.2025406/1‑150 (MQ=60)
     TGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTA                                                                                                                                                 >  SRR19973063.3965428/1‑150 (MQ=60)
      GTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAA                                                                                                                                                >  SRR19973063.1771621/1‑150 (MQ=60)
       TTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAA                                                                                                                                               <  SRR19973063.3622898/150‑1 (MQ=60)
         GCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGA                                                                                                                                             >  SRR19973063.379175/1‑150 (MQ=60)
            GGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCG                                                                                                                                          >  SRR19973063.3577640/1‑150 (MQ=60)
              AAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTC                                                                                                                                        >  SRR19973063.1893956/1‑150 (MQ=60)
              AAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTC                                                                                                                                        >  SRR19973063.317637/1‑150 (MQ=60)
               AAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGCATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCT                                                                                                                                       <  SRR19973063.3825427/150‑1 (MQ=60)
               AAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCT                                                                                                                                       >  SRR19973063.257934/1‑150 (MQ=60)
               AAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCT                                                                                                                                       >  SRR19973063.2246029/1‑150 (MQ=60)
               AAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATAAACGATCGTCT                                                                                                                                       >  SRR19973063.553521/1‑150 (MQ=60)
                  ACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGA                                                                                                                                    >  SRR19973063.2446465/1‑150 (MQ=60)
                    GCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGCTCGTCTGGAAA                                                                                                                                  <  SRR19973063.895044/150‑1 (MQ=60)
                    GCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAA                                                                                                                                  <  SRR19973063.1230188/150‑1 (MQ=60)
                    GCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAA                                                                                                                                  <  SRR19973063.2958698/150‑1 (MQ=60)
                    GCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAA                                                                                                                                  <  SRR19973063.894873/150‑1 (MQ=60)
                     CAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAA                                                                                                                                 <  SRR19973063.1929040/150‑1 (MQ=60)
                     CAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAA                                                                                                                                 >  SRR19973063.2852966/1‑150 (MQ=60)
                        AACCATCGGTATGCGTGTGCCTTTTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACA                                                                                                                              <  SRR19973063.4137439/150‑1 (MQ=60)
                        AACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACA                                                                                                                              <  SRR19973063.4137308/150‑1 (MQ=60)
                         ACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAG                                                                                                                             <  SRR19973063.1090812/150‑1 (MQ=60)
                          CCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGG                                                                                                                            <  SRR19973063.1588319/150‑1 (MQ=60)
                           CATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGT                                                                                                                           <  SRR19973063.3663975/150‑1 (MQ=60)
                           CATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGT                                                                                                                           <  SRR19973063.123995/150‑1 (MQ=60)
                           CATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGT                                                                                                                           >  SRR19973063.3062416/1‑150 (MQ=60)
                            ATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTA                                                                                                                          <  SRR19973063.1430582/150‑1 (MQ=60)
                             TCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAG                                                                                                                         <  SRR19973063.2612958/150‑1 (MQ=60)
                             TCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAG                                                                                                                         >  SRR19973063.4276331/1‑150 (MQ=60)
                             TCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAG                                                                                                                         >  SRR19973063.4276254/1‑150 (MQ=60)
                              CGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGA                                                                                                                        >  SRR19973063.3040594/1‑150 (MQ=60)
                              CGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGA                                                                                                                        >  SRR19973063.3040555/1‑150 (MQ=60)
                               GGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGAT                                                                                                                       >  SRR19973063.2495401/1‑150 (MQ=60)
                               GGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGAT                                                                                                                       >  SRR19973063.2495869/1‑150 (MQ=60)
                                GTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATT                                                                                                                      >  SRR19973063.1191228/1‑150 (MQ=60)
                                GTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATT                                                                                                                      >  SRR19973063.774543/1‑150 (MQ=60)
                                 TATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGCAAGATTT                                                                                                                     >  SRR19973063.4428317/1‑150 (MQ=60)
                                   TGCGTGTGCCTTCTAACCCTCTCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGA                                                                                                                   <  SRR19973063.3194918/150‑1 (MQ=60)
                                   TGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGA                                                                                                                   >  SRR19973063.4920869/1‑150 (MQ=60)
                                    GCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGAT                                                                                                                  <  SRR19973063.3759885/150‑1 (MQ=60)
                                     CGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGAGT                                                                                                                 >  SRR19973063.4799708/1‑150 (MQ=60)
                                       TGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTAT                                                                                                               <  SRR19973063.3701936/150‑1 (MQ=60)
                                       TGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTAT                                                                                                               <  SRR19973063.4085866/150‑1 (MQ=60)
                                            CTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACG                                                                                                          <  SRR19973063.1679520/150‑1 (MQ=60)
                                              TCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGC                                                                                                        >  SRR19973063.1366360/1‑150 (MQ=60)
                                              TCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGC                                                                                                        >  SRR19973063.4319500/1‑150 (MQ=60)
                                                 AACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGC                                                                                                     >  SRR19973063.969931/1‑150 (MQ=60)
                                                   CCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTA                                                                                                   >  SRR19973063.2953787/1‑150 (MQ=60)
                                                   CCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTA                                                                                                   >  SRR19973063.3287490/1‑150 (MQ=60)
                                                   CCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGACTGGAAAAACAGGTAGATTTGATAATTCACGGCTGCTA                                                                                                   >  SRR19973063.2953806/1‑150 (MQ=60)
                                                     CTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACNGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATC                                                                                                 >  SRR19973063.2684914/1‑150 (MQ=60)
                                                      TATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCT                                                                                                >  SRR19973063.462920/1‑150 (MQ=60)
                                                        TCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCG                                                                                              >  SRR19973063.1580819/1‑150 (MQ=60)
                                                         CGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGG                                                                                             >  SRR19973063.1112832/1‑150 (MQ=60)
                                                            TCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCA                                                                                          <  SRR19973063.4866978/150‑1 (MQ=60)
                                                              AGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGA                                                                                        >  SRR19973063.1703025/1‑150 (MQ=60)
                                                                 CGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAAC                                                                                     <  SRR19973063.4352772/150‑1 (MQ=60)
                                                                  GTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTCTCTCGGGCAGAAACC                                                                                    >  SRR19973063.3528276/1‑150 (MQ=60)
                                                                  GTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACC                                                                                    >  SRR19973063.3528326/1‑150 (MQ=60)
                                                                   TTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCG                                                                                   >  SRR19973063.3094979/1‑150 (MQ=60)
                                                                   TTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCG                                                                                   >  SRR19973063.3426212/1‑150 (MQ=60)
                                                                     ACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGAC                                                                                 >  SRR19973063.328761/1‑150 (MQ=60)
                                                                       TTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAA                                                                               >  SRR19973063.4387929/1‑150 (MQ=60)
                                                                       TTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAA                                                                               >  SRR19973063.4387845/1‑150 (MQ=60)
                                                                         GAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACG                                                                             >  SRR19973063.2515663/1‑150 (MQ=60)
                                                                          AAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACTGCGGCTATCTCGGGCAGAAACCGACAAAGG                                                                            >  SRR19973063.1354361/1‑150 (MQ=60)
                                                                           AGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGT                                                                           <  SRR19973063.1008422/150‑1 (MQ=60)
                                                                           AGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGT                                                                           >  SRR19973063.4372589/1‑150 (MQ=60)
                                                                           AGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGT                                                                           >  SRR19973063.4372580/1‑150 (MQ=60)
                                                                            GCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTT                                                                          <  SRR19973063.419182/150‑1 (MQ=60)
                                                                            GCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTT                                                                          <  SRR19973063.4958092/150‑1 (MQ=60)
                                                                              ATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTAT                                                                        <  SRR19973063.4339819/150‑1 (MQ=60)
                                                                              ATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTAT                                                                        <  SRR19973063.2919342/150‑1 (MQ=60)
                                                                               TTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATT                                                                       >  SRR19973063.627383/1‑150 (MQ=60)
                                                                                TGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTG                                                                      >  SRR19973063.2097757/1‑150 (MQ=60)
                                                                                    CGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCT                                                                  >  SRR19973063.3252574/1‑150 (MQ=60)
                                                                                     GAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTT                                                                 >  SRR19973063.2028116/1‑150 (MQ=60)
                                                                                     GAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTT                                                                 >  SRR19973063.2031833/1‑150 (MQ=60)
                                                                                     GAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTT                                                                 >  SRR19973063.2829840/1‑150 (MQ=60)
                                                                                       ACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTAC                                                               >  SRR19973063.4312939/1‑150 (MQ=60)
                                                                                          GATGTTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGA                                                            >  SRR19973063.3134651/1‑150 (MQ=60)
                                                                                          GATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGA                                                            >  SRR19973063.1201166/1‑150 (MQ=60)
                                                                                          GATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGA                                                            >  SRR19973063.1200954/1‑150 (MQ=60)
                                                                                            TGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATG                                                          <  SRR19973063.1273213/150‑1 (MQ=60)
                                                                                               TTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATA                                                       <  SRR19973063.1797104/150‑1 (MQ=60)
                                                                                               TTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGGTATAGATCTTACCGATGATA                                                       >  SRR19973063.3572639/1‑150 (MQ=60)
                                                                                                TTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATAC                                                      <  SRR19973063.3216177/150‑1 (MQ=60)
                                                                                                   CACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTGGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCC                                                   >  SRR19973063.296756/1‑150 (MQ=60)
                                                                                                   CACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCC                                                   >  SRR19973063.1027624/1‑150 (MQ=60)
                                                                                                    ACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCG                                                  <  SRR19973063.3560647/150‑1 (MQ=60)
                                                                                                        CTCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCG                                              >  SRR19973063.1957589/1‑150 (MQ=60)
                                                                                                        CTCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCG                                              >  SRR19973063.4407317/1‑150 (MQ=60)
                                                                                                            AATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGT                                          <  SRR19973063.3998590/150‑1 (MQ=60)
                                                                                                            AATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGT                                          <  SRR19973063.1235630/150‑1 (MQ=60)
                                                                                                            AATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGT                                          <  SRR19973063.869230/150‑1 (MQ=60)
                                                                                                             ATGCTGCCAGGCAGCGAATTTACCGAATCGGGCCCGGAAGAAAATAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTG                                         >  SRR19973063.4777837/1‑150 (MQ=60)
                                                                                                               GCTGCCCGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCG                                       <  SRR19973063.2680173/150‑1 (MQ=60)
                                                                                                                 TGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTG                                     >  SRR19973063.2874935/1‑150 (MQ=60)
                                                                                                                 TGCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTG                                     >  SRR19973063.4046749/1‑150 (MQ=60)
                                                                                                                 TGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTG                                     <  SRR19973063.2320788/150‑1 (MQ=60)
                                                                                                                  GCCAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGA                                    <  SRR19973063.4561976/150‑1 (MQ=60)
                                                                                                                    CAGGCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAG                                  >  SRR19973063.4168151/1‑150 (MQ=60)
                                                                                                                       GCAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCG                               <  SRR19973063.5061873/150‑1 (MQ=60)
                                                                                                                        CAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGT                              >  SRR19973063.900335/1‑150 (MQ=60)
                                                                                                                        CAGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGT                              >  SRR19973063.900439/1‑150 (MQ=60)
                                                                                                                         AGCGAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTA                             >  SRR19973063.2826481/1‑150 (MQ=60)
                                                                                                                            GAATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGT                          >  SRR19973063.4223784/1‑150 (MQ=60)
                                                                                                                             AATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTG                         <  SRR19973063.4084987/150‑1 (MQ=60)
                                                                                                                              ATTTACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGA                        >  SRR19973063.321423/1‑150 (MQ=60)
                                                                                                                               TTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGAT                       <  SRR19973063.1726865/150‑1 (MQ=60)
                                                                                                                               TTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGAT                       <  SRR19973063.2708646/150‑1 (MQ=60)
                                                                                                                                TTACCGAATCGGACCCGGAATAAATTAAAGATCGTCGGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATG                      <  SRR19973063.1365138/150‑1 (MQ=60)
                                                                                                                                 TACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGT                     <  SRR19973063.1919365/150‑1 (MQ=60)
                                                                                                                                  ACCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTG                    >  SRR19973063.3422838/1‑150 (MQ=60)
                                                                                                                                   CCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGA                   <  SRR19973063.899635/150‑1 (MQ=60)
                                                                                                                                   CCGAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGA                   <  SRR19973063.899577/150‑1 (MQ=60)
                                                                                                                                     GAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAG                 <  SRR19973063.4641703/150‑1 (MQ=60)
                                                                                                                                     GAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAG                 >  SRR19973063.140443/1‑150 (MQ=60)
                                                                                                                                     GAATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAG                 <  SRR19973063.4641878/150‑1 (MQ=60)
                                                                                                                                      AATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGC                <  SRR19973063.3043446/150‑1 (MQ=60)
                                                                                                                                       ATCGGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCC               <  SRR19973063.1510279/150‑1 (MQ=60)
                                                                                                                                          GGACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTT            <  SRR19973063.2259846/150‑1 (MQ=60)
                                                                                                                                          GGACCCAGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTAGTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTT            >  SRR19973063.700829/1‑150 (MQ=60)
                                                                                                                                           GACCCGGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTC           <  SRR19973063.1031082/150‑1 (MQ=60)
                                                                                                                                           GACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATGTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGAGTAGGTGATGTGAAGCCTTTC           >  SRR19973063.813553/1‑150 (MQ=60)
                                                                                                                                                GGAATAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATA      <  SRR19973063.1464919/150‑1 (MQ=60)
                                                                                                                                                GGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATA      <  SRR19973063.4548569/150‑1 (MQ=60)
                                                                                                                                                    TAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAA  >  SRR19973063.4701529/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
CCGTCTGTTGCAGGAAAAACGCAAAACCATCGGTATGCGTGTGCCTTCTAACCCTATCGCTCAGGCGTTACTTGAAGCATTGGGCGAACCGATGCTTTCCACTTCGCTAATGCTGCCAGGCAGCGAATTTACCGAATCGGACCCGGAAGAAATTAAAGATCGTCTGGAAAAACAGGTAGATTTGATTATTCACGGCGGCTATCTCGGGCAGAAACCGACAACGGTTATTGATCTTACCGATGATACGCCGGTCGTGGTGCGTGAAGGCGTAGGTGATGTGAAGCCTTTCTTATAACAA  >  CP009273/1318681‑1318978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑