Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,830,311 G→T 100% intergenic (+395/‑2) yicT → / → setC pseudogene/putative arabinose efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,830,3110GT99.5% 705.2 / ‑4.8 212intergenic (+395/‑2)yicT/setCpseudogene/putative arabinose efflux transporter
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (107/104);  total (107/105)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 218 total aligned reads displayed.

CTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTGAAAGCCCGTC  >  CP009273/3830164‑3830457
                                                                                                                                                   |                                                                                                                                                  
cTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAgaataa                                                                                                                                                  >  2:3004474/1‑150 (MQ=255)
    taatCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCa                                                                                                                                              <  1:4259869/150‑1 (MQ=255)
    taatCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCa                                                                                                                                              >  1:2346020/1‑150 (MQ=255)
    taatCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCa                                                                                                                                              >  1:2345774/1‑150 (MQ=255)
      atCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCaaa                                                                                                                                            >  1:3702690/1‑150 (MQ=255)
       tctcAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCaaaa                                                                                                                                           >  2:2057926/1‑150 (MQ=255)
          cAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAAc                                                                                                                                        >  2:2756733/1‑150 (MQ=255)
             ttGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGc                                                                                                                                     >  1:808753/1‑150 (MQ=255)
             ttGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGc                                                                                                                                     <  1:1101740/150‑1 (MQ=255)
               gCTGGCCCTATAATATTGAGATAGCTTTGCTTTCAGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGNTa                                                                                                                                   <  1:1984172/150‑1 (MQ=255)
                 tGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTAcc                                                                                                                                 >  2:3320355/1‑150 (MQ=255)
                 tGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTAGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTAcc                                                                                                                                 >  2:564018/1‑150 (MQ=255)
                  ggCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCa                                                                                                                                <  2:4237076/150‑1 (MQ=255)
                     ccTATAATATTGAGCTAGAATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTc                                                                                                                             <  2:3178986/150‑1 (MQ=255)
                         taataTTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATc                                                                                                                         >  2:2994093/1‑150 (MQ=255)
                          aataTTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCa                                                                                                                        <  2:4923081/150‑1 (MQ=255)
                            tatTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCaaa                                                                                                                      >  2:186035/1‑150 (MQ=255)
                             atTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCaaaa                                                                                                                     >  2:1161368/1‑150 (MQ=255)
                               tgagcTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAAt                                                                                                                   >  2:4148176/4‑150 (MQ=255)
                                gagcTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATa                                                                                                                  <  1:3194785/148‑1 (MQ=255)
                                 agcTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATAc                                                                                                                 >  1:2215691/2‑150 (MQ=255)
                                  gcTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACt                                                                                                                <  1:918026/150‑1 (MQ=255)
                                  gcTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACt                                                                                                                <  1:3008517/150‑1 (MQ=255)
                                  gcTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACt                                                                                                                <  2:4769312/150‑1 (MQ=255)
                                    tAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTg                                                                                                              >  2:4241690/1‑150 (MQ=255)
                                       cATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGAtc                                                                                                           <  2:1850200/150‑1 (MQ=255)
                                        aTTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGAtct                                                                                                          <  2:1756499/150‑1 (MQ=255)
                                        aTTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGAtct                                                                                                          <  2:1756511/150‑1 (MQ=255)
                                          tGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCa                                                                                                        >  1:294434/1‑150 (MQ=255)
                                          tGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCa                                                                                                        <  2:126161/150‑1 (MQ=255)
                                           gCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCAc                                                                                                       <  1:3180933/150‑1 (MQ=255)
                                               tCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGcc                                                                                                   >  1:2727665/1‑150 (MQ=255)
                                               tCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGcc                                                                                                   >  2:358610/1‑150 (MQ=255)
                                               tCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGcc                                                                                                   <  1:4942902/150‑1 (MQ=255)
                                                cTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCcg                                                                                                  <  1:1290713/150‑1 (MQ=255)
                                                 tGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCcgc                                                                                                 >  1:5138536/1‑150 (MQ=255)
                                                 tGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCcgc                                                                                                 >  1:1906416/1‑150 (MQ=255)
                                                     tGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATAATTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCa                                                                                             <  2:1240227/150‑1 (MQ=255)
                                                     tGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATAATTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCa                                                                                             <  2:1240156/150‑1 (MQ=255)
                                                     tGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATAATTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCa                                                                                             <  2:4299188/150‑1 (MQ=255)
                                                      gTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCAt                                                                                            >  1:2140100/1‑150 (MQ=255)
                                                         tCAGCGATACTCAAAACTTCTTAATACACTACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCAtttt                                                                                         <  2:2953754/150‑1 (MQ=255)
                                                         tCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCAtttt                                                                                         >  2:3487707/1‑150 (MQ=255)
                                                                tACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGt                                                                                  >  2:2325587/1‑150 (MQ=255)
                                                                 aCTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTc                                                                                 >  2:1160518/1‑150 (MQ=255)
                                                                           tCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGa                                                                       <  1:2065869/150‑1 (MQ=255)
                                                                              tAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACgg                                                                    >  1:2722709/1‑150 (MQ=255)
                                                                                 tacaatacTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTa                                                                 >  1:1569368/1‑150 (MQ=255)
                                                                                  acaatacTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTAt                                                                <  2:2862318/150‑1 (MQ=255)
                                                                                   caatacTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTAtt                                                               <  2:4939342/150‑1 (MQ=255)
                                                                                     atacTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGc                                                             >  2:4230401/1‑150 (MQ=255)
                                                                                       acTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCgg                                                           >  1:2619443/1‑150 (MQ=255)
                                                                                       acTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCgg                                                           >  1:667520/1‑150 (MQ=255)
                                                                                          cATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGgcg                                                        >  1:4461110/1‑150 (MQ=255)
                                                                                             tCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGctc                                                     >  1:1328942/1‑150 (MQ=255)
                                                                                             ccGACTGGTCCTTATTTGTAACTCAGCTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGctc                                                     <  1:4687879/149‑1 (MQ=255)
                                                                                              cGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCTGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGctct                                                    >  1:3626723/1‑150 (MQ=255)
                                                                                              cGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGctct                                                    <  1:2628622/150‑1 (MQ=255)
                                                                                               gtcTGGTTCTTATTTGTATCTCAGTTATATTTTTTCGCCCGGTGATTAAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGTCGCTCtt                                                   <  1:1209112/148‑1 (MQ=255)
                                                                                                aCTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTc                                                  <  1:4622822/150‑1 (MQ=255)
                                                                                                aCTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTc                                                  >  1:1447287/1‑150 (MQ=255)
                                                                                                 cTGGTACTTAATTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAACAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCa                                                 <  1:4751145/150‑1 (MQ=255)
                                                                                                  tGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAg                                                >  2:137961/1‑150 (MQ=255)
                                                                                                  tGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAg                                                >  2:4263089/1‑150 (MQ=255)
                                                                                                  tGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAg                                                <  1:2348121/150‑1 (MQ=255)
                                                                                                    gTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGAc                                              <  1:3731080/150‑1 (MQ=255)
                                                                                                    gTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGAc                                              <  2:307220/150‑1 (MQ=255)
                                                                                                       cTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTcc                                           >  1:4768572/1‑150 (MQ=255)
                                                                                                        ttATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCt                                          <  2:1495761/150‑1 (MQ=255)
                                                                                                          aTTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTAc                                        <  2:833741/150‑1 (MQ=255)
                                                                                                          aTTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTAc                                        >  1:2882177/1‑150 (MQ=255)
                                                                                                            ttGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTAccc                                      <  2:1787309/150‑1 (MQ=255)
                                                                                                                aaCTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAg                                  <  1:83719/150‑1 (MQ=255)
                                                                                                                aaCTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAg                                  <  2:3715505/150‑1 (MQ=255)
                                                                                                                  cTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGta                                >  2:403569/1‑150 (MQ=255)
                                                                                                                   tCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGtat                               <  1:3788676/150‑1 (MQ=255)
                                                                                                                     aGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTatat                             >  1:1714210/1‑150 (MQ=255)
                                                                                                                        tataTTTTTTCGCCCTGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTcc                          >  2:2350563/1‑150 (MQ=255)
                                                                                                                        tataTTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTcc                          <  2:4593386/150‑1 (MQ=255)
                                                                                                                         atatTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCt                         >  1:2750171/1‑150 (MQ=255)
                                                                                                                          tatTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTc                        <  2:281775/150‑1 (MQ=255)
                                                                                                                          tatTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTc                        <  1:2036504/150‑1 (MQ=255)
                                                                                                                          tatTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTc                        >  2:4438274/1‑150 (MQ=255)
                                                                                                                          tatTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTc                        <  1:3901772/150‑1 (MQ=255)
                                                                                                                           atTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCg                       <  2:2718578/150‑1 (MQ=255)
                                                                                                                              ttttCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAg                    >  2:1226741/1‑150 (MQ=255)
                                                                                                                               tttCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGa                   >  1:529084/1‑150 (MQ=255)
                                                                                                                                 tCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATg                 >  1:2537117/1‑150 (MQ=255)
                                                                                                                                 tCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATg                 >  1:176739/1‑150 (MQ=255)
                                                                                                                                  cGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGa                <  2:2273450/150‑1 (MQ=255)
                                                                                                                                  cGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGa                <  2:764976/150‑1 (MQ=255)
                                                                                                                                    cccGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAAc              <  2:2739056/150‑1 (MQ=255)
                                                                                                                                     ccGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACt             <  1:358610/150‑1 (MQ=255)
                                                                                                                                      cGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTg            <  1:4176878/150‑1 (MQ=255)
                                                                                                                                      cGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTg            >  2:4786664/1‑150 (MQ=255)
                                                                                                                                       ggTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTGa           >  2:3617361/1‑150 (MQ=255)
                                                                                                                                             tCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTGAAAGccc     <  2:2441835/150‑1 (MQ=255)
                                                                                                                                              cAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTGAAAGCCCg    <  2:4477278/150‑1 (MQ=255)
                                                                                                                                                gaataaTGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTGAAAGCCCGTc  >  2:1583397/1‑150 (MQ=255)
                                                                                                                                                gaataaTGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTGAAAGCCCGTc  <  2:2770358/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTGAAAGCCCGTC  >  CP009273/3830164‑3830457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTGAAAGCCCGTCCTA  >  CP009273/3830163‑3830460
                                                                                                                                                    |                                                                                                                                                     
ACTAATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATA                                                                                                                                                      >  SRR19973063.2747902/1‑150 (MQ=60)
   AATAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATG                                                                                                                                                   <  SRR19973063.1507537/150‑1 (MQ=60)
     TAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCA                                                                                                                                                 >  SRR19973063.2353393/1‑150 (MQ=60)
     TAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCA                                                                                                                                                 >  SRR19973063.1900269/1‑150 (MQ=60)
     TAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCA                                                                                                                                                 >  SRR19973063.2353527/1‑150 (MQ=60)
     TAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCA                                                                                                                                                 >  SRR19973063.2353281/1‑150 (MQ=60)
     TAATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCA                                                                                                                                                 <  SRR19973063.4273426/150‑1 (MQ=60)
      AATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAA                                                                                                                                                >  SRR19973063.3701993/1‑150 (MQ=60)
       ATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAA                                                                                                                                               >  SRR19973063.3714537/1‑150 (MQ=60)
       ATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAA                                                                                                                                               <  SRR19973063.3120935/150‑1 (MQ=60)
       ATCTCAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAA                                                                                                                                               >  SRR19973063.3011599/1‑150 (MQ=60)
           CAATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAAC                                                                                                                                           <  SRR19973063.3733860/150‑1 (MQ=60)
            AATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACG                                                                                                                                          <  SRR19973063.1378763/150‑1 (MQ=60)
             ATTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGG                                                                                                                                         >  SRR19973063.2063702/1‑150 (MQ=60)
              TTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGC                                                                                                                                        >  SRR19973063.811091/1‑150 (MQ=60)
              TTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGC                                                                                                                                        >  SRR19973063.811083/1‑150 (MQ=60)
              TTGCTGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGC                                                                                                                                        <  SRR19973063.1105097/150‑1 (MQ=60)
                GCTGGCCCTATAATATTGAGATAGCTTTGCTTTCAGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGNTA                                                                                                                                      <  SRR19973063.1990373/150‑1 (MQ=60)
                 CTGGCCCTATAATATAGAGCTAGCATTTCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACATGTACTTATTTGTAACTCATTTATATTTTTTCGCACGGTGATTCAGAATAATGCAAAAAAATGCTAC                                                                                                                                     >  SRR19973063.5114721/1‑150 (MQ=60)
                  TGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACC                                                                                                                                    <  SRR19973063.2304228/150‑1 (MQ=60)
                  TGGCCCTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACC                                                                                                                                    >  SRR19973063.7089/1‑150 (MQ=60)
                       CTATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCC                                                                                                                               >  SRR19973063.4730375/1‑150 (MQ=60)
                        TATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCA                                                                                                                              <  SRR19973063.1716931/150‑1 (MQ=60)
                         ATAATATTGAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCAT                                                                                                                             <  SRR19973063.911158/150‑1 (MQ=60)
                                 GAGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATA                                                                                                                     <  SRR19973063.3205056/150‑1 (MQ=60)
                                  AGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATAC                                                                                                                    >  SRR19973063.2222677/1‑150 (MQ=60)
                                   GCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACT                                                                                                                   <  SRR19973063.3018117/150‑1 (MQ=60)
                                   GCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACT                                                                                                                   <  SRR19973063.920732/150‑1 (MQ=60)
                                    CTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTT                                                                                                                  <  SRR19973063.4579423/150‑1 (MQ=60)
                                     TAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTG                                                                                                                 >  SRR19973063.3949070/1‑150 (MQ=60)
                                         ATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGTCCGGCGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCT                                                                                                             >  SRR19973063.4055399/1‑150 (MQ=60)
                                         ATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCT                                                                                                             >  SRR19973063.1325253/1‑150 (MQ=60)
                                           TGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCA                                                                                                           <  SRR19973063.65152/150‑1 (MQ=60)
                                           TGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCA                                                                                                           >  SRR19973063.295306/1‑150 (MQ=60)
                                            GCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCAC                                                                                                          <  SRR19973063.3191161/150‑1 (MQ=60)
                                             CTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACT                                                                                                         >  SRR19973063.4228977/1‑150 (MQ=60)
                                                TCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCTCCCGCTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCC                                                                                                      >  SRR19973063.3484909/1‑150 (MQ=60)
                                                TCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCC                                                                                                      >  SRR19973063.2736469/1‑150 (MQ=60)
                                                TCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCC                                                                                                      <  SRR19973063.4958890/150‑1 (MQ=60)
                                                 CTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCG                                                                                                     <  SRR19973063.1294553/150‑1 (MQ=60)
                                                  TGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGC                                                                                                    >  SRR19973063.1912379/1‑150 (MQ=60)
                                                  TGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGC                                                                                                    >  SRR19973063.5155062/1‑150 (MQ=60)
                                                     TTGTATCAGCGATAATAAAAAATTCTTAATACAATACTCATTCGACTGGTAATTATTTGTATCTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGC                                                                                                 <  SRR19973063.4853135/150‑1 (MQ=60)
                                                       GTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCAT                                                                                               >  SRR19973063.2146781/1‑150 (MQ=60)
                                                        TATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATT                                                                                              <  SRR19973063.2214474/150‑1 (MQ=60)
                                                              CGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACT                                                                                        <  SRR19973063.273355/150‑1 (MQ=60)
                                                                  ACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCAACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTC                                                                                    >  SRR19973063.2604015/1‑150 (MQ=60)
                                                                        AAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTT                                                                              >  SRR19973063.1751423/1‑150 (MQ=60)
                                                                        AAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTT                                                                              >  SRR19973063.2793885/1‑150 (MQ=60)
                                                                         AATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTC                                                                             >  SRR19973063.2894939/1‑150 (MQ=60)
                                                                            TCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGA                                                                          <  SRR19973063.4659452/150‑1 (MQ=60)
                                                                            TCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGA                                                                          <  SRR19973063.2072327/150‑1 (MQ=60)
                                                                               TAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGG                                                                       >  SRR19973063.2731500/1‑150 (MQ=60)
                                                                                  TACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTA                                                                    >  SRR19973063.1574185/1‑150 (MQ=60)
                                                                                   ACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTCT                                                                   >  SRR19973063.200030/1‑150 (MQ=60)
                                                                                     AATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTG                                                                 <  SRR19973063.1223930/150‑1 (MQ=60)
                                                                                        ACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGG                                                              >  SRR19973063.669470/1‑150 (MQ=60)
                                                                                        ACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGG                                                              >  SRR19973063.2627783/1‑150 (MQ=60)
                                                                                         CTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGG                                                             >  SRR19973063.3197270/1‑150 (MQ=60)
                                                                                           CATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCG                                                           >  SRR19973063.4475424/1‑150 (MQ=60)
                                                                                              TCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTC                                                        >  SRR19973063.1332920/1‑150 (MQ=60)
                                                                                              CCGACTGGTCCTTATTTGTAACTCAGCTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTC                                                        <  SRR19973063.4702824/150‑1 (MQ=60)
                                                                                               CGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCTGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCT                                                       >  SRR19973063.3638366/1‑150 (MQ=60)
                                                                                               CGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCT                                                       <  SRR19973063.2637004/150‑1 (MQ=60)
                                                                                               AGACTGGTTCTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTGGTCGCCTTTCTGACGGGTATTGCGGGCGCTCT                                                       <  SRR19973063.2636065/150‑1 (MQ=60)
                                                                                                GTCTGGTTCTTATTTGTATCTCAGTTATATTTTTTCGCCCGGTGATTAAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGTCGCTCTT                                                      <  SRR19973063.1212735/150‑1 (MQ=60)
                                                                                                 ACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTC                                                     >  SRR19973063.242109/1‑150 (MQ=60)
                                                                                                 ACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTC                                                     >  SRR19973063.1451703/1‑150 (MQ=60)
                                                                                                 ACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTC                                                     <  SRR19973063.4637593/150‑1 (MQ=60)
                                                                                                 ACTGGTACTTATTTGTAACTCAGTTATATATTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTC                                                     <  SRR19973063.2743104/150‑1 (MQ=60)
                                                                                                  CTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCA                                                    <  SRR19973063.4766508/150‑1 (MQ=60)
                                                                                                  CTGGTACTTAATTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAACAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCA                                                    <  SRR19973063.4766286/150‑1 (MQ=60)
                                                                                                   TGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAG                                                   <  SRR19973063.2355598/150‑1 (MQ=60)
                                                                                                   TGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAG                                                   >  SRR19973063.3531087/1‑150 (MQ=60)
                                                                                                   TGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAG                                                   <  SRR19973063.2355634/150‑1 (MQ=60)
                                                                                                     GTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGAC                                                 <  SRR19973063.3742996/150‑1 (MQ=60)
                                                                                                     GTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGAC                                                 <  SRR19973063.3743505/150‑1 (MQ=60)
                                                                                                        CTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCC                                              >  SRR19973063.4783787/1‑150 (MQ=60)
                                                                                                           ATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTAC                                           >  SRR19973063.2891392/1‑150 (MQ=60)
                                                                                                           ATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTAC                                           >  SRR19973063.2116963/1‑150 (MQ=60)
                                                                                                            TTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACC                                          >  SRR19973063.4339973/1‑150 (MQ=60)
                                                                                                              TGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCT                                        >  SRR19973063.1929708/1‑150 (MQ=60)
                                                                                                                TAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAA                                      <  SRR19973063.1186916/150‑1 (MQ=60)
                                                                                                                 AACTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAG                                     <  SRR19973063.84009/150‑1 (MQ=60)
                                                                                                                   CTCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTA                                   <  SRR19973063.239390/150‑1 (MQ=60)
                                                                                                                    TCAGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTAT                                  <  SRR19973063.3800759/150‑1 (MQ=60)
                                                                                                                      AGTTATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATAT                                >  SRR19973063.1719438/1‑150 (MQ=60)
                                                                                                                         TATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCC                             <  SRR19973063.2657763/150‑1 (MQ=60)
                                                                                                                          ATATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCT                            >  SRR19973063.2759043/1‑150 (MQ=60)
                                                                                                                           TATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTC                           <  SRR19973063.3914201/150‑1 (MQ=60)
                                                                                                                           TATTTTTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTC                           <  SRR19973063.2042868/150‑1 (MQ=60)
                                                                                                                                TTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGA                      >  SRR19973063.530636/1‑150 (MQ=60)
                                                                                                                                TTTCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGA                      >  SRR19973063.530604/1‑150 (MQ=60)
                                                                                                                                  TCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATG                    >  SRR19973063.2545205/1‑150 (MQ=60)
                                                                                                                                  TCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATG                    >  SRR19973063.177285/1‑150 (MQ=60)
                                                                                                                                  TCGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGACTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATG                    >  SRR19973063.2780588/1‑150 (MQ=60)
                                                                                                                                   CGCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTAATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGA                   >  SRR19973063.1613137/1‑150 (MQ=60)
                                                                                                                                    GCCCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAA                  <  SRR19973063.2122760/150‑1 (MQ=60)
                                                                                                                                      CCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACT                <  SRR19973063.359727/150‑1 (MQ=60)
                                                                                                                                      CCGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACT                <  SRR19973063.359642/150‑1 (MQ=60)
                                                                                                                                       CGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTG               <  SRR19973063.4190222/150‑1 (MQ=60)
                                                                                                                                       CGGTGATTCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTG               <  SRR19973063.4748366/150‑1 (MQ=60)
                                                                                                                                              TCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTGAAAGCCC        >  SRR19973063.736972/1‑150 (MQ=60)
                                                                                                                                              TCAGAATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTGAAAGCCC        >  SRR19973063.3905230/1‑150 (MQ=60)
                                                                                                                                                  AATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTGAAAGCCCGTCC    >  SRR19973063.1728119/1‑150 (MQ=60)
                                                                                                                                                  AATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTGAAAGCCCGTCC    >  SRR19973063.1727944/1‑150 (MQ=60)
                                                                                                                                                  AATAATGCAAAAAACGGCTACCACTCCATAAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCAACCCTAAGTATATTCCTCGCAGATGAACTGAAAGCCCGTCC    >  SRR19973063.1727914/1‑150 (MQ=60)
                                                                                                                                                   ATAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTGAAAGCCCGTCCT   >  SRR19973063.806420/1‑150 (MQ=60)
                                                                                                                                                    TAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCATATGAAATGAAAGCCCGTCCTA  >  SRR19973063.1555409/1‑150 (MQ=60)
                                                                                                                                                    TAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTGAAAGCCCGTCCTA  <  SRR19973063.3324205/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
ACTAATAATCTCAATTGCTGGCCCTATAATATTGCGCTAGCATTGCTTTCTGGTTGTATCAGCGATACTCAAAAATTCTTAATACAATACTCATTCGACTGGTACTTATTTGTAACTCAGTTATATTTTTTCGCCCGGTGATTCAGAAGAATGCAAAAAACGGCTACCACTCCATCAAAAATACTTGATCTCACTGCCGCGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGAACTGAAAGCCCGTCCTA  >  CP009273/3830163‑3830460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑