Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,242,509 C→T 100% L26F (CTC→TTC)  ubiC → chorismate‑‑pyruvate lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,242,5090CT98.2% 936.9 / 3.6 282L26F (CTC→TTC) ubiCchorismate‑‑pyruvate lyase
Reads supporting (aligned to +/- strand):  ref base C (4/1);  new base T (135/141);  total (140/142)
Fisher's exact test for biased strand distribution p-value = 2.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 282 total aligned reads displayed.

ATTTGCTTTACATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGCTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCGTTA  >  CP009273/4242362‑4242654
                                                                                                                                                   |                                                                                                                                                 
 tttGCTTTACATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCg                                                                                                                                                >  2:4512189/1‑150 (MQ=255)
 tttGCTTTACATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCg                                                                                                                                                >  1:4597871/1‑150 (MQ=255)
     cTTTACATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTg                                                                                                                                            >  2:3169578/1‑150 (MQ=255)
         aCATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTg                                                                                                                                        >  1:838603/1‑150 (MQ=255)
         aCATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTg                                                                                                                                        >  2:1290114/1‑150 (MQ=255)
         aCATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTg                                                                                                                                        >  1:2606559/1‑150 (MQ=255)
          cATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGt                                                                                                                                       >  2:4883714/1‑150 (MQ=255)
           aTCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGtt                                                                                                                                      <  1:2704908/150‑1 (MQ=255)
             ctcCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGc                                                                                                                                    >  2:1951263/1‑150 (MQ=255)
               cccGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTg                                                                                                                                  <  2:92375/150‑1 (MQ=255)
               cccGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTg                                                                                                                                  <  2:3038728/150‑1 (MQ=255)
               cccGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTg                                                                                                                                  <  2:3131488/150‑1 (MQ=255)
                ccGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTgg                                                                                                                                 <  2:2238027/150‑1 (MQ=255)
                 cGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTgga                                                                                                                                >  1:2933099/1‑150 (MQ=255)
                     aaCACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGAt                                                                                                                            <  1:3789727/150‑1 (MQ=255)
                        acTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTcc                                                                                                                         <  1:1677579/150‑1 (MQ=255)
                         cTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCa                                                                                                                        <  2:740472/150‑1 (MQ=255)
                          ttttCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCAt                                                                                                                       <  2:2075929/150‑1 (MQ=255)
                          ttttCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCAt                                                                                                                       >  1:4736625/1‑150 (MQ=255)
                           tttCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGTCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATg                                                                                                                      >  2:2607270/1‑150 (MQ=255)
                           tttCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATg                                                                                                                      <  2:4217430/150‑1 (MQ=255)
                             tCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGAc                                                                                                                    <  1:228415/150‑1 (MQ=255)
                                  gATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAAc                                                                                                               >  2:1277883/1‑150 (MQ=255)
                                       aaTGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGtttt                                                                                                          <  1:2191006/150‑1 (MQ=255)
                                       aaTGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGtttt                                                                                                          <  1:4393126/150‑1 (MQ=255)
                                        aTGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTg                                                                                                         >  2:1015468/1‑150 (MQ=255)
                                         tGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGa                                                                                                        <  1:4859787/150‑1 (MQ=255)
                                          gCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGaa                                                                                                       >  2:1364152/1‑150 (MQ=255)
                                          gCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGaa                                                                                                       >  1:1088413/1‑150 (MQ=255)
                                             tttACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAAcag                                                                                                    <  2:4283578/150‑1 (MQ=255)
                                                  gTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAggg                                                                                               <  2:1053808/150‑1 (MQ=255)
                                                   ttATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGa                                                                                              <  1:1277883/150‑1 (MQ=255)
                                                   ttATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGa                                                                                              >  2:574450/1‑150 (MQ=255)
                                                    tATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGaa                                                                                             <  2:2452435/150‑1 (MQ=255)
                                                      tGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGaaaa                                                                                           >  1:302276/1‑150 (MQ=255)
                                                        tAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAAc                                                                                         >  1:1482089/1‑150 (MQ=255)
                                                        tAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAAc                                                                                         <  2:3536512/150‑1 (MQ=255)
                                                         aaCGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTTTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACg                                                                                        <  1:1390918/150‑1 (MQ=255)
                                                          aCGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACgg                                                                                       <  1:72333/150‑1 (MQ=255)
                                                             gagaGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTaa                                                                                    <  2:602539/150‑1 (MQ=255)
                                                                agTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCg                                                                                 >  1:5112602/1‑150 (MQ=255)
                                                                agTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCg                                                                                 <  2:2018641/150‑1 (MQ=255)
                                                                 gTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGt                                                                                >  1:4934439/1‑150 (MQ=255)
                                                                  ttCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTg                                                                               >  1:5150653/1‑150 (MQ=255)
                                                                    cGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTCAGCGTGAc                                                                             >  2:66851/1‑150 (MQ=255)
                                                                    cGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGAc                                                                             <  1:4494012/150‑1 (MQ=255)
                                                                     ggCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACg                                                                            >  1:2328936/1‑150 (MQ=255)
                                                                     ggCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACg                                                                            <  1:1709177/150‑1 (MQ=255)
                                                                       cATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACgat                                                                          >  1:179597/1‑150 (MQ=255)
                                                                          gTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGAGCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACgatgat                                                                       <  1:1914679/150‑1 (MQ=255)
                                                                           tCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATc                                                                      <  2:1681228/150‑1 (MQ=255)
                                                                           tCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATc                                                                      >  2:1137504/1‑150 (MQ=255)
                                                                            cacaCCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATcc                                                                     <  1:2008467/150‑1 (MQ=255)
                                                                               acCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCcgcg                                                                  >  2:3811118/1‑150 (MQ=255)
                                                                                ccccGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGa                                                                 >  2:4116339/1‑150 (MQ=255)
                                                                                  ccGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAg                                                               <  2:1435827/150‑1 (MQ=255)
                                                                                   cgcgTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAgg                                                              <  2:2752032/150‑1 (MQ=255)
                                                                                     cgTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGt                                                            >  1:1047999/1‑150 (MQ=255)
                                                                                            gCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGTTGATCCGCGAAGGGTTTGTCGa                                                     <  2:1517586/150‑1 (MQ=255)
                                                                                            gCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGa                                                     <  2:505456/150‑1 (MQ=255)
                                                                                              taCTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGc                                                   >  2:3345052/2‑150 (MQ=255)
                                                                                              aaCTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGc                                                   >  1:1151596/1‑150 (MQ=255)
                                                                                                 tgcgtgcgCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGa                                                >  1:429404/1‑150 (MQ=255)
                                                                                                 tgcgtgcgCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGa                                                >  2:2548047/1‑150 (MQ=255)
                                                                                                  gcgtgcgCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGaa                                               >  1:1536786/1‑150 (MQ=255)
                                                                                                    gtgcgCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTTTTCGACTGGCTGTTGCTGGAGGATTCCTTGACAAAACGTTTTGAACAGCAGGGAAAAACTGTAAGCGTGACGATGATCCGCGAAGGTTTTGTCGAGCAGATTg                                             <  1:4125826/150‑1 (MQ=255)
                                                                                                     tgcgctgcgcTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGa                                            <  2:3980680/150‑1 (MQ=255)
                                                                                                      gcgctgcgctATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGaa                                           <  1:4354135/150‑1 (MQ=255)
                                                                                                       cgctgcgctATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGaaa                                          >  1:3946929/1‑150 (MQ=255)
                                                                                                       cgctgcgctATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGaaa                                          >  2:4141636/1‑150 (MQ=255)
                                                                                                       cgctgcgctATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGaaa                                          >  2:4806530/1‑150 (MQ=255)
                                                                                                       cgctgcgctATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGaaa                                          >  2:392456/1‑150 (MQ=255)
                                                                                                        gctgcgctATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAAt                                         >  2:2666497/1‑150 (MQ=255)
                                                                                                        gctgcgctATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAAt                                         >  2:2913282/1‑150 (MQ=255)
                                                                                                                aTTGTAAAGAGATCCCTGCCCTGGATCCGCAACAGTTCGACTGGCTCTAGCTGTAGGATTCCATGACAAAACGTTTTGAACAGCAGTGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGATTGTCGAGAAGAATGAAATCCCCgaag                                 >  1:2753191/1‑150 (MQ=255)
                                                                                                                     aaaGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTg                            <  1:4358217/150‑1 (MQ=255)
                                                                                                                         agaTCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCgc                        >  2:2469495/1‑150 (MQ=255)
                                                                                                                           aTCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCgctg                      <  2:368671/150‑1 (MQ=255)
                                                                                                                               ctgccctgGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCtgctgc                  <  1:1598655/150‑1 (MQ=255)
                                                                                                                                tgccctgGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGcc                 >  2:4353405/1‑150 (MQ=255)
                                                                                                                                 gccctgGATCCGCTACTGTTCGACTGGCTGTTGCTGGAGTATTCCATGAAAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCTCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCg                <  1:3661228/150‑1 (MQ=255)
                                                                                                                                   cctgGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGac              >  2:4861873/1‑149 (MQ=255)
                                                                                                                                    ctgGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAATCGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGaaa             >  2:93204/1‑150 (MQ=255)
                                                                                                                                     tgGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAag            >  1:862144/1‑150 (MQ=255)
                                                                                                                                      ggATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAaga           <  2:4451998/150‑1 (MQ=255)
                                                                                                                                      ggATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAaga           <  2:1881709/150‑1 (MQ=255)
                                                                                                                                       gATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAagag          <  2:179597/150‑1 (MQ=255)
                                                                                                                                        aTCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGt         >  1:3200680/1‑150 (MQ=255)
                                                                                                                                         tCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGtc        <  1:4187581/150‑1 (MQ=255)
                                                                                                                                         tCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGtc        <  1:512212/150‑1 (MQ=255)
                                                                                                                                           cGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGtctc      <  2:4249989/150‑1 (MQ=255)
                                                                                                                                            gCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCg     <  1:5028307/150‑1 (MQ=255)
                                                                                                                                            gCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCg     <  1:3304993/150‑1 (MQ=255)
                                                                                                                                              aaCTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCGtt   <  2:3455717/150‑1 (MQ=255)
                                                                                                                                              aaCTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCGtt   <  1:3345052/150‑1 (MQ=255)
                                                                                                                                              aaCTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCGtt   <  2:1159102/150‑1 (MQ=255)
                                                                                                                                              aaCTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCGtt   >  1:2855619/1‑150 (MQ=255)
                                                                                                                                              aaCTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCGtt   >  2:2255836/1‑150 (MQ=255)
                                                                                                                                               aCTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCGTTa  >  2:4597199/1‑150 (MQ=255)
                                                                                                                                               aCTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCGTTa  >  2:853847/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ATTTGCTTTACATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGCTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCGTTA  >  CP009273/4242362‑4242654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTGCTTTACATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGCTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCGTTACTG  >  CP009273/4242363‑4242657
                                                                                                                                                  |                                                                                                                                                    
TTTGCTTTACATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCG                                                                                                                                                   >  SRR19973063.4612627/1‑150 (MQ=60)
TTTGCTTTACATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCG                                                                                                                                                   >  SRR19973063.4612583/1‑150 (MQ=60)
        ACATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTG                                                                                                                                           >  SRR19973063.841027/1‑150 (MQ=60)
        ACATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTG                                                                                                                                           >  SRR19973063.2614840/1‑150 (MQ=60)
         CATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGT                                                                                                                                          >  SRR19973063.2152434/1‑150 (MQ=60)
          ATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTT                                                                                                                                         <  SRR19973063.2713638/150‑1 (MQ=60)
          ATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTT                                                                                                                                         <  SRR19973063.2713750/150‑1 (MQ=60)
           TCTCCAGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTG                                                                                                                                        <  SRR19973063.2391398/150‑1 (MQ=60)
              CCCGTAAACACTTTTCTGCGATACAATGCCTGTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTG                                                                                                                                     <  SRR19973063.4861449/150‑1 (MQ=60)
                CGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGA                                                                                                                                   >  SRR19973063.4901155/1‑150 (MQ=60)
                CGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGA                                                                                                                                   >  SRR19973063.2942450/1‑150 (MQ=60)
                  TAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGG                                                                                                                                 <  SRR19973063.1878608/150‑1 (MQ=60)
                    AACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTTGAGGAT                                                                                                                               <  SRR19973063.3801532/150‑1 (MQ=60)
                    AACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGAT                                                                                                                               <  SRR19973063.3801814/150‑1 (MQ=60)
                    AACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGAT                                                                                                                               <  SRR19973063.749510/150‑1 (MQ=60)
                       ACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCC                                                                                                                            <  SRR19973063.1682692/150‑1 (MQ=60)
                       ACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCC                                                                                                                            <  SRR19973063.4438329/150‑1 (MQ=60)
                         TTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCAT                                                                                                                          <  SRR19973063.3951656/150‑1 (MQ=60)
                         TTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCAT                                                                                                                          >  SRR19973063.4751714/1‑150 (MQ=60)
                         TTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCAT                                                                                                                          <  SRR19973063.1611372/150‑1 (MQ=60)
                           TTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGA                                                                                                                        <  SRR19973063.1089684/150‑1 (MQ=60)
                            TCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGAC                                                                                                                       <  SRR19973063.229134/150‑1 (MQ=60)
                            TCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGAC                                                                                                                       <  SRR19973063.229100/150‑1 (MQ=60)
                             CTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACA                                                                                                                      >  SRR19973063.1734083/1‑150 (MQ=60)
                             CTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACA                                                                                                                      >  SRR19973063.1734147/1‑150 (MQ=60)
                               GCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAA                                                                                                                    <  SRR19973063.4097069/150‑1 (MQ=60)
                                 GATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAAC                                                                                                                  >  SRR19973063.3484485/1‑150 (MQ=60)
                                 GATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAAC                                                                                                                  <  SRR19973063.4631590/150‑1 (MQ=60)
                                  ATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACG                                                                                                                 >  SRR19973063.3147123/1‑150 (MQ=60)
                                     CAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTT                                                                                                              >  SRR19973063.2263729/1‑150 (MQ=60)
                                      AATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTT                                                                                                             <  SRR19973063.4407193/150‑1 (MQ=60)
                                      AATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTT                                                                                                             <  SRR19973063.2197885/150‑1 (MQ=60)
                                       ATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTG                                                                                                            >  SRR19973063.4399391/1‑150 (MQ=60)
                                       ATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTG                                                                                                            <  SRR19973063.3565459/150‑1 (MQ=60)
                                        TGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGA                                                                                                           <  SRR19973063.4875444/150‑1 (MQ=60)
                                         GCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAA                                                                                                          >  SRR19973063.1091723/1‑150 (MQ=60)
                                         GCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAA                                                                                                          >  SRR19973063.2977092/1‑150 (MQ=60)
                                          CCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAAC                                                                                                         <  SRR19973063.3340344/150‑1 (MQ=60)
                                          CCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAAC                                                                                                         <  SRR19973063.3725419/150‑1 (MQ=60)
                                          CCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAAC                                                                                                         <  SRR19973063.3340416/150‑1 (MQ=60)
                                              TACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCA                                                                                                     >  SRR19973063.1470891/1‑150 (MQ=60)
                                                 GTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGG                                                                                                  >  SRR19973063.853243/1‑150 (MQ=60)
                                                 GTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGG                                                                                                  >  SRR19973063.2878117/1‑150 (MQ=60)
                                                 GTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGG                                                                                                  >  SRR19973063.574148/1‑150 (MQ=60)
                                                  TTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAATAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGA                                                                                                 <  SRR19973063.3313065/150‑1 (MQ=60)
                                                  TTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGA                                                                                                 <  SRR19973063.1281694/150‑1 (MQ=60)
                                                     TGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAA                                                                                              >  SRR19973063.303165/1‑150 (MQ=60)
                                                       TAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAAC                                                                                            >  SRR19973063.1486624/1‑150 (MQ=60)
                                                       TAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAAC                                                                                            <  SRR19973063.1683043/150‑1 (MQ=60)
                                                        AACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTTTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACG                                                                                           <  SRR19973063.1395126/150‑1 (MQ=60)
                                                        AACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACG                                                                                           <  SRR19973063.1714265/150‑1 (MQ=60)
                                                         ACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGG                                                                                          <  SRR19973063.72586/150‑1 (MQ=60)
                                                          CGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGT                                                                                         <  SRR19973063.1172534/150‑1 (MQ=60)
                                                          CGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGT                                                                                         <  SRR19973063.1247943/150‑1 (MQ=60)
                                                            GAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAA                                                                                       >  SRR19973063.1116970/1‑150 (MQ=60)
                                                             AGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCCTGACAAAACGTTTTGAACAGCAGGGACAAACGGTAAG                                                                                      >  SRR19973063.2990857/1‑150 (MQ=60)
                                                               AGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCG                                                                                    >  SRR19973063.5129051/1‑150 (MQ=60)
                                                               AGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCG                                                                                    >  SRR19973063.2572542/1‑150 (MQ=60)
                                                                GTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGT                                                                                   <  SRR19973063.1585045/150‑1 (MQ=60)
                                                                GTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGT                                                                                   >  SRR19973063.4950380/1‑150 (MQ=60)
                                                                 TTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTG                                                                                  >  SRR19973063.5167228/1‑150 (MQ=60)
                                                                  TCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGA                                                                                 >  SRR19973063.275065/1‑150 (MQ=60)
                                                                   CGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGAC                                                                                <  SRR19973063.4508428/150‑1 (MQ=60)
                                                                   CGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGAC                                                                                <  SRR19973063.5129698/150‑1 (MQ=60)
                                                                   CGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGAC                                                                                <  SRR19973063.5129657/150‑1 (MQ=60)
                                                                    GGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACG                                                                               >  SRR19973063.2336389/1‑150 (MQ=60)
                                                                    GGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACG                                                                               >  SRR19973063.4904647/1‑150 (MQ=60)
                                                                    GGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACG                                                                               <  SRR19973063.1714382/150‑1 (MQ=60)
                                                                      CATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGAT                                                                             >  SRR19973063.180321/1‑150 (MQ=60)
                                                                      CATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGAT                                                                             >  SRR19973063.180151/1‑150 (MQ=60)
                                                                         GTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGAGCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGAT                                                                          <  SRR19973063.1920670/150‑1 (MQ=60)
                                                                           CACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCC                                                                        >  SRR19973063.1522238/1‑150 (MQ=60)
                                                                           CACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCC                                                                        <  SRR19973063.2014739/150‑1 (MQ=60)
                                                                            ACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCG                                                                       <  SRR19973063.1416320/150‑1 (MQ=60)
                                                                                CCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAA                                                                   <  SRR19973063.4995153/150‑1 (MQ=60)
                                                                                    CGTTAACGCAACTGCGTGCGCTGCGCTATTGTACTGAGATCCCTGCCGGGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGT                                                               >  SRR19973063.2324506/1‑150 (MQ=60)
                                                                                    CGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGT                                                               >  SRR19973063.3893158/1‑150 (MQ=60)
                                                                                    CGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGT                                                               >  SRR19973063.1051173/1‑150 (MQ=60)
                                                                                         ACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTC                                                          <  SRR19973063.1923510/150‑1 (MQ=60)
                                                                                         ACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTC                                                          <  SRR19973063.436673/150‑1 (MQ=60)
                                                                                         ACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTC                                                          <  SRR19973063.5142229/150‑1 (MQ=60)
                                                                                           GCAACTGCGTGCGCTGCGATATTGTAAAGAGATCCCTGCCCTGGATCCGCAACAGTTCGACTGGATGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGTCGATGATCCGAGATGGGTTTGTCGA                                                        >  SRR19973063.4805943/1‑150 (MQ=60)
                                                                                             AACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGC                                                      >  SRR19973063.1155075/1‑150 (MQ=60)
                                                                                             AACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGC                                                      <  SRR19973063.3066792/150‑1 (MQ=60)
                                                                                                TGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGA                                                   >  SRR19973063.430599/1‑150 (MQ=60)
                                                                                                TGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGA                                                   >  SRR19973063.433939/1‑150 (MQ=60)
                                                                                                 GCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAA                                                  >  SRR19973063.1541492/1‑150 (MQ=60)
                                                                                                   GTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTTTTCGACTGGCTGTTGCTGGAGGATTCCTTGACAAAACGTTTTGAACAGCAGGGAAAAACTGTAAGCGTGACGATGATCCGCGAAGGTTTTGTCGAGCAGATTG                                                <  SRR19973063.4139035/150‑1 (MQ=60)
                                                                                                     GCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAA                                              <  SRR19973063.4368043/150‑1 (MQ=60)
                                                                                                      CGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAA                                             >  SRR19973063.3959554/1‑150 (MQ=60)
                                                                                                           CGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCC                                        <  SRR19973063.4164784/150‑1 (MQ=60)
                                                                                                           CGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCC                                        <  SRR19973063.5126370/150‑1 (MQ=60)
                                                                                                             CTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGA                                      >  SRR19973063.1141882/1‑150 (MQ=60)
                                                                                                               ATTGTAAAGAGATCCCTGCCCTGGATCCGCAACAGTTCGACTGGCTCTAGCTGTAGGATTCCATGACAAAACGTTTTGAACAGCAGTGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGATTGTCGAGAAGAATGAAATCCCCGAAG                                    >  SRR19973063.2762071/1‑150 (MQ=60)
                                                                                                                 TGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAA                                  <  SRR19973063.563939/150‑1 (MQ=60)
                                                                                                                   TAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACT                                >  SRR19973063.1655536/1‑150 (MQ=60)
                                                                                                                   TAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACT                                <  SRR19973063.943678/150‑1 (MQ=60)
                                                                                                                    AAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTG                               <  SRR19973063.4372098/150‑1 (MQ=60)
                                                                                                                    AAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTG                               <  SRR19973063.4372148/150‑1 (MQ=60)
                                                                                                                       GAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCG                            >  SRR19973063.2077599/1‑150 (MQ=60)
                                                                                                                            CCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCT                       >  SRR19973063.756092/1‑150 (MQ=60)
                                                                                                                             CCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTG                      <  SRR19973063.962730/150‑1 (MQ=60)
                                                                                                                              CTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGC                     <  SRR19973063.1603538/150‑1 (MQ=60)
                                                                                                                              CTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGC                     <  SRR19973063.1603557/150‑1 (MQ=60)
                                                                                                                              CTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGC                     <  SRR19973063.511640/150‑1 (MQ=60)
                                                                                                                                GCCCTGGATCCGCTACTGTTCGACTGGCTGTTGCTGGAGTATTCCATGAAAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCTCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCG                   <  SRR19973063.3672967/150‑1 (MQ=60)
                                                                                                                                GCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCG                   <  SRR19973063.4313950/150‑1 (MQ=60)
                                                                                                                                 CCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGA                  >  SRR19973063.1391307/1‑150 (MQ=60)
                                                                                                                                 CCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGA                  >  SRR19973063.1391653/1‑150 (MQ=60)
                                                                                                                                    TGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAG               >  SRR19973063.4583626/1‑150 (MQ=60)
                                                                                                                                    TGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAG               >  SRR19973063.864627/1‑150 (MQ=60)
                                                                                                                                    TGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAG               >  SRR19973063.4583663/1‑150 (MQ=60)
                                                                                                                                     GGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGA              >  SRR19973063.2802909/1‑150 (MQ=60)
                                                                                                                                     GGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGA              >  SRR19973063.2802910/1‑150 (MQ=60)
                                                                                                                                       ATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGT            >  SRR19973063.4207490/1‑150 (MQ=60)
                                                                                                                                       ATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGT            >  SRR19973063.3210969/1‑150 (MQ=60)
                                                                                                                                        TCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTC           <  SRR19973063.4200947/150‑1 (MQ=60)
                                                                                                                                        TCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTC           <  SRR19973063.4200954/150‑1 (MQ=60)
                                                                                                                                        TCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTC           <  SRR19973063.513722/150‑1 (MQ=60)
                                                                                                                                        TCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTC           <  SRR19973063.513711/150‑1 (MQ=60)
                                                                                                                                         CCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCT          <  SRR19973063.4235477/150‑1 (MQ=60)
                                                                                                                                         CCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCT          <  SRR19973063.4386676/150‑1 (MQ=60)
                                                                                                                                           GCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCG        <  SRR19973063.3315565/150‑1 (MQ=60)
                                                                                                                                           GCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCG        <  SRR19973063.5044524/150‑1 (MQ=60)
                                                                                                                                             AACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCGTT      >  SRR19973063.2865028/1‑150 (MQ=60)
                                                                                                                                             AACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCGTT      >  SRR19973063.2864770/1‑150 (MQ=60)
                                                                                                                                             AACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCGTT      <  SRR19973063.2615554/150‑1 (MQ=60)
                                                                                                                                             AACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCGTT      <  SRR19973063.3355713/150‑1 (MQ=60)
                                                                                                                                              ACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCGTTA     >  SRR19973063.2231215/1‑150 (MQ=60)
                                                                                                                                                 GTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCGTTACTG  >  SRR19973063.3324654/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                    
TTTGCTTTACATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGCTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCGTTACTG  >  CP009273/4242363‑4242657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑