Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,242,509 C→T 100% L26F (CTC→TTC)  ubiC → chorismate‑‑pyruvate lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,242,5090CT98.7% 732.5 / ‑0.0 225L26F (CTC→TTC) ubiCchorismate‑‑pyruvate lyase
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base T (117/105);  total (118/107)
Fisher's exact test for biased strand distribution p-value = 6.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 225 total aligned reads displayed.

TTGCTTTACATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGCTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCGTT  >  CP009273/4242364‑4242653
                                                                                                                                                 |                                                                                                                                                
ttGCTTTACATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTc                                                                                                                                                >  1:917327/1‑148 (MQ=255)
   cTTTACATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGAc                                                                                                                                             <  2:1924620/148‑1 (MQ=255)
     ttACATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGGTATGTAACGGAGACTAGGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTg                                                                                                                                           >  1:3746535/1‑148 (MQ=255)
       aCATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGc                                                                                                                                         >  2:2271692/1‑148 (MQ=255)
        cATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCt                                                                                                                                        >  1:170371/1‑148 (MQ=255)
          tctcCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGt                                                                                                                                      >  2:136250/1‑148 (MQ=255)
          tctcCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGt                                                                                                                                      >  2:1823998/1‑148 (MQ=255)
           ctcCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGtt                                                                                                                                     >  1:3452198/1‑148 (MQ=255)
           ctcCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGtt                                                                                                                                     >  2:2124617/1‑148 (MQ=255)
            tcCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTg                                                                                                                                    <  2:1409763/148‑1 (MQ=255)
               cGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTg                                                                                                                                 <  2:1958959/148‑1 (MQ=255)
                gTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTgg                                                                                                                                <  1:3420785/148‑1 (MQ=255)
                gTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTgg                                                                                                                                <  1:136218/148‑1 (MQ=255)
                gTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTgg                                                                                                                                <  1:1163035/148‑1 (MQ=255)
                 tAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTgga                                                                                                                               >  2:2667798/1‑148 (MQ=255)
                 tAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTgga                                                                                                                               <  2:3634527/148‑1 (MQ=255)
                 tAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTgga                                                                                                                               >  2:41321/1‑148 (MQ=255)
                  aaaCACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTggag                                                                                                                              >  2:3413648/1‑148 (MQ=255)
                  aaaCACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTggag                                                                                                                              >  2:873240/1‑148 (MQ=255)
                   aaCACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTggagg                                                                                                                             <  2:1902470/148‑1 (MQ=255)
                    acacTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTggagga                                                                                                                            <  2:391803/148‑1 (MQ=255)
                    acacTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTggagga                                                                                                                            >  1:3054185/1‑148 (MQ=255)
                      acTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGAtt                                                                                                                          >  1:3624539/1‑148 (MQ=255)
                      acTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGAtt                                                                                                                          <  2:1726926/148‑1 (MQ=255)
                      acTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGCTCGACTGGCTGTTGCTGGAGGAtt                                                                                                                          <  1:1868513/148‑1 (MQ=255)
                       cTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTc                                                                                                                         <  2:2796902/148‑1 (MQ=255)
                        ttttCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTcc                                                                                                                        >  1:800266/1‑148 (MQ=255)
                           tCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATg                                                                                                                     <  1:1910103/148‑1 (MQ=255)
                                   aCAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACg                                                                                                             >  1:253147/1‑148 (MQ=255)
                                    cAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGt                                                                                                            >  2:88404/1‑148 (MQ=255)
                                    cAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGt                                                                                                            <  1:1107665/148‑1 (MQ=255)
                                    cAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGt                                                                                                            <  1:1729447/148‑1 (MQ=255)
                                     aaTGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGtt                                                                                                           <  2:2963765/148‑1 (MQ=255)
                                      aTGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGttt                                                                                                          <  2:568289/148‑1 (MQ=255)
                                       tGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGtttt                                                                                                         >  1:1094994/1‑148 (MQ=255)
                                           tttACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAAc                                                                                                     >  2:3471339/1‑148 (MQ=255)
                                            ttACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAAca                                                                                                    >  2:33450/1‑148 (MQ=255)
                                              aCGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAAcagc                                                                                                  <  2:1759349/148‑1 (MQ=255)
                                                 ttATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAgg                                                                                               >  2:2699412/1‑148 (MQ=255)
                                                 ttATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAgg                                                                                               >  2:3728972/1‑148 (MQ=255)
                                                     gTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGaaa                                                                                           >  2:3249010/1‑148 (MQ=255)
                                                      tAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGaaaa                                                                                          <  1:2901217/148‑1 (MQ=255)
                                                       aaCGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGaaaaa                                                                                         <  2:104918/148‑1 (MQ=255)
                                                        aCGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAAc                                                                                        >  2:1264180/1‑148 (MQ=255)
                                                         cGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACg                                                                                       >  2:1062957/1‑148 (MQ=255)
                                                         cGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACg                                                                                       >  2:215862/1‑148 (MQ=255)
                                                           gagaGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGt                                                                                     <  1:3454816/148‑1 (MQ=255)
                                                            agagTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTa                                                                                    <  1:3116761/148‑1 (MQ=255)
                                                              agTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAg                                                                                  <  1:3026273/148‑1 (MQ=255)
                                                              agTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAg                                                                                  >  1:1571710/1‑148 (MQ=255)
                                                                ttCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCg                                                                                >  1:1194266/1‑148 (MQ=255)
                                                                  cGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTg                                                                              <  2:2846924/148‑1 (MQ=255)
                                                                     cATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACg                                                                           <  1:986915/148‑1 (MQ=255)
                                                                      aTGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACga                                                                          <  1:2101004/148‑1 (MQ=255)
                                                                      aTGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACga                                                                          >  1:487707/1‑148 (MQ=255)
                                                                       tGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACgat                                                                         >  1:316220/1‑148 (MQ=255)
                                                                        gTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACgatg                                                                        <  2:313284/148‑1 (MQ=255)
                                                                          cacaCCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACgatgat                                                                      >  2:1395836/1‑148 (MQ=255)
                                                                          cacaCCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACgatgat                                                                      >  1:2380280/1‑148 (MQ=255)
                                                                                   cgTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAgg                                                             >  2:1478668/1‑148 (MQ=255)
                                                                                    gTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAggg                                                            <  1:1991864/148‑1 (MQ=255)
                                                                                    gTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAggg                                                            <  1:147160/148‑1 (MQ=255)
                                                                                     ttAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGt                                                           <  1:2737866/148‑1 (MQ=255)
                                                                                     ttAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGt                                                           <  2:914017/148‑1 (MQ=255)
                                                                                      tAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGtt                                                          >  2:696660/1‑148 (MQ=255)
                                                                                         cGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGTCTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGt                                                       <  1:2271692/148‑1 (MQ=255)
                                                                                         cGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGt                                                       >  1:655220/1‑148 (MQ=255)
                                                                                            aaCTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGa                                                    >  1:603791/1‑148 (MQ=255)
                                                                                             aCTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAg                                                   <  1:2476829/148‑1 (MQ=255)
                                                                                             aCTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAg                                                   <  2:2179734/148‑1 (MQ=255)
                                                                                                 cgtgcgCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGa                                               <  1:3605890/148‑1 (MQ=255)
                                                                                                  gtgcgCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGaa                                              <  2:3746535/148‑1 (MQ=255)
                                                                                                       ctgcgctATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGaaa                                         <  1:3200781/148‑1 (MQ=255)
                                                                                                              aTTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCga                                  >  1:2820610/1‑148 (MQ=255)
                                                                                                               ttGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCgaa                                 >  1:3279023/1‑148 (MQ=255)
                                                                                                               ttGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCgaa                                 >  1:1376997/1‑148 (MQ=255)
                                                                                                                 gTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCgaaga                               <  2:2454771/148‑1 (MQ=255)
                                                                                                                    aaGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACt                            >  1:454085/1‑148 (MQ=255)
                                                                                                                     agagATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTg                           <  2:3022036/148‑1 (MQ=255)
                                                                                                                     agagATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTg                           <  1:439107/148‑1 (MQ=255)
                                                                                                                     agagATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTg                           <  1:874276/148‑1 (MQ=255)
                                                                                                                      gagaTCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGc                          <  1:1690594/148‑1 (MQ=255)
                                                                                                                       agaTCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGcc                         >  1:2936095/1‑148 (MQ=255)
                                                                                                                            cctgccctgGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCgctgc                    >  1:2296321/1‑148 (MQ=255)
                                                                                                                            cctgccctgGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCgctgc                    >  1:3130881/1‑148 (MQ=255)
                                                                                                                             ctgccctgGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCgctgct                   >  2:2352191/1‑148 (MQ=255)
                                                                                                                             ctgccctgGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCgctgct                   >  2:428386/1‑148 (MQ=255)
                                                                                                                             ctgccctgGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCgctgct                   <  1:1258930/148‑1 (MQ=255)
                                                                                                                              tgccctgGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGctgctg                  >  1:535540/1‑148 (MQ=255)
                                                                                                                               gccctgGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCtgctgc                 <  1:41321/148‑1 (MQ=255)
                                                                                                                                  ctgGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGa              >  1:519561/1‑148 (MQ=255)
                                                                                                                                     gATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAag           >  2:507018/1‑148 (MQ=255)
                                                                                                                                     gATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAag           >  2:59059/1‑148 (MQ=255)
                                                                                                                                      aTCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAaga          <  2:410357/148‑1 (MQ=255)
                                                                                                                                      aTCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAaga          <  1:383683/148‑1 (MQ=255)
                                                                                                                                       tCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAagag         >  2:1611212/1‑148 (MQ=255)
                                                                                                                                        ccGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGt        >  2:1166603/1‑148 (MQ=255)
                                                                                                                                        ccGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGt        >  2:963960/1‑148 (MQ=255)
                                                                                                                                          gCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGtct      <  2:1754721/148‑1 (MQ=255)
                                                                                                                                              cTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCGtt  >  1:261338/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTGCTTTACATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGCTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCGTT  >  CP009273/4242364‑4242653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATTTGCTTTACATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGCTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCGTTACTGG  >  CP009273/4242361‑4242658
                                                                                                                                                    |                                                                                                                                                     
AATTTGCTTTACATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTT                                                                                                                                                      >  SRR19973069.2471273/1‑150 (MQ=60)
 ATTTGCTTTACATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTC                                                                                                                                                     <  SRR19973069.1714403/150‑1 (MQ=60)
 ATTTGCTTTACATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTC                                                                                                                                                     >  SRR19973069.923583/1‑150 (MQ=60)
 ATTTGCTTTACATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTC                                                                                                                                                     >  SRR19973069.825168/1‑150 (MQ=60)
   TTGCTTTACATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGA                                                                                                                                                   >  SRR19973069.3571721/1‑150 (MQ=60)
   TTGCTTTACATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGA                                                                                                                                                   >  SRR19973069.927253/1‑150 (MQ=60)
      CTTTACATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTATGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTG                                                                                                                                                >  SRR19973069.3769962/1‑150 (MQ=60)
      CTTTACATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGGTATGTAACGGAGACTAGGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTG                                                                                                                                                >  SRR19973069.3770939/1‑150 (MQ=60)
         TACATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCT                                                                                                                                             >  SRR19973069.171825/1‑150 (MQ=60)
            ATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTT                                                                                                                                          <  SRR19973069.700048/150‑1 (MQ=60)
            ATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTT                                                                                                                                          >  SRR19973069.3474580/1‑150 (MQ=60)
              CTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGC                                                                                                                                        >  SRR19973069.1310389/1‑150 (MQ=60)
                   GTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAG                                                                                                                                   <  SRR19973069.137483/150‑1 (MQ=60)
                   GTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAG                                                                                                                                   <  SRR19973069.137515/150‑1 (MQ=60)
                   GTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAG                                                                                                                                   <  SRR19973069.3443049/150‑1 (MQ=60)
                   GTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAG                                                                                                                                   <  SRR19973069.1171118/150‑1 (MQ=60)
                     AAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGA                                                                                                                                 >  SRR19973069.3074392/1‑150 (MQ=60)
                     AAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGA                                                                                                                                 >  SRR19973069.3074466/1‑150 (MQ=60)
                      AACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGAT                                                                                                                                >  SRR19973069.421374/1‑150 (MQ=60)
                      AACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGAT                                                                                                                                >  SRR19973069.1713429/1‑150 (MQ=60)
                      AACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGAT                                                                                                                                >  SRR19973069.1767082/1‑150 (MQ=60)
                       ACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATT                                                                                                                               >  SRR19973069.3648227/1‑150 (MQ=60)
                        CACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTC                                                                                                                              <  SRR19973069.498357/150‑1 (MQ=60)
                        CACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTC                                                                                                                              <  SRR19973069.498508/150‑1 (MQ=60)
                         ACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCC                                                                                                                             >  SRR19973069.880416/1‑150 (MQ=60)
                         ACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCC                                                                                                                             >  SRR19973069.805937/1‑150 (MQ=60)
                         ACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGCTCGACTGGCTGTTGCTGGAGGATTCC                                                                                                                             <  SRR19973069.1881532/150‑1 (MQ=60)
                              TCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGAC                                                                                                                        <  SRR19973069.1923286/150‑1 (MQ=60)
                               CTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACA                                                                                                                       <  SRR19973069.1903439/150‑1 (MQ=60)
                                    ATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACG                                                                                                                  >  SRR19973069.251988/1‑150 (MQ=60)
                                    ATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACG                                                                                                                  >  SRR19973069.254996/1‑150 (MQ=60)
                                       CAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTT                                                                                                               >  SRR19973069.35011/1‑150 (MQ=60)
                                       CAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTT                                                                                                               <  SRR19973069.1115363/150‑1 (MQ=60)
                                       CAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTT                                                                                                               <  SRR19973069.1115392/150‑1 (MQ=60)
                                       CAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTT                                                                                                               <  SRR19973069.1741567/150‑1 (MQ=60)
                                        AATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTT                                                                                                              >  SRR19973069.1102646/1‑150 (MQ=60)
                                        AATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTT                                                                                                              >  SRR19973069.2261281/1‑150 (MQ=60)
                                        AATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTT                                                                                                              >  SRR19973069.2462874/1‑150 (MQ=60)
                                                         TAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAAC                                                                                             <  SRR19973069.2920379/150‑1 (MQ=60)
                                                         TAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAAC                                                                                             <  SRR19973069.2069064/150‑1 (MQ=60)
                                                              GAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAA                                                                                        <  SRR19973069.3477204/150‑1 (MQ=60)
                                                               AGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAG                                                                                       >  SRR19973069.1582402/1‑150 (MQ=60)
                                                               AGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAG                                                                                       <  SRR19973069.3137243/150‑1 (MQ=60)
                                                                 AGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCG                                                                                     >  SRR19973069.1202489/1‑150 (MQ=60)
                                                                 AGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCG                                                                                     <  SRR19973069.3046345/150‑1 (MQ=60)
                                                                 AGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGCTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCG                                                                                     >  SRR19973069.3630676/1‑150 (MQ=60)
                                                                 AGTTCGGCATGTCACACCCCGCGTTAACGAAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCG                                                                                     <  SRR19973069.3809362/150‑1 (MQ=60)
                                                                  GTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGT                                                                                    >  SRR19973069.1457182/1‑150 (MQ=60)
                                                                     CGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGAC                                                                                 <  SRR19973069.1167609/150‑1 (MQ=60)
                                                                      GGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACG                                                                                <  SRR19973069.2685690/150‑1 (MQ=60)
                                                                       GCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGA                                                                               >  SRR19973069.491226/1‑150 (MQ=60)
                                                                        CATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGAT                                                                              >  SRR19973069.318719/1‑150 (MQ=60)
                                                                        CATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGAT                                                                              >  SRR19973069.318309/1‑150 (MQ=60)
                                                                        CATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGAT                                                                              <  SRR19973069.993603/150‑1 (MQ=60)
                                                                         ATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCTACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATG                                                                             <  SRR19973069.174223/150‑1 (MQ=60)
                                                                         ATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATG                                                                             <  SRR19973069.2115512/150‑1 (MQ=60)
                                                                           GTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGAT                                                                           >  SRR19973069.2396424/1‑150 (MQ=60)
                                                                              ACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCG                                                                        >  SRR19973069.2270508/1‑150 (MQ=60)
                                                                                  CCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAA                                                                    >  SRR19973069.535145/1‑150 (MQ=60)
                                                                                    CGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGG                                                                  <  SRR19973069.3508691/150‑1 (MQ=60)
                                                                                      CGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGT                                                                <  SRR19973069.759842/150‑1 (MQ=60)
                                                                                       GTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTT                                                               <  SRR19973069.148473/150‑1 (MQ=60)
                                                                                       GTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTT                                                               <  SRR19973069.2005747/150‑1 (MQ=60)
                                                                                        TTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGTTCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTT                                                              <  SRR19973069.2757153/150‑1 (MQ=60)
                                                                                        TTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTT                                                              <  SRR19973069.2756177/150‑1 (MQ=60)
                                                                                          AACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGT                                                            >  SRR19973069.659615/1‑150 (MQ=60)
                                                                                           ACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTC                                                           <  SRR19973069.2951614/150‑1 (MQ=60)
                                                                                           ACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTC                                                           <  SRR19973069.3134773/150‑1 (MQ=60)
                                                                                            CGCAACTGCGTGCGCTGCGCTATTGGAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGTCTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCG                                                          <  SRR19973069.2287099/150‑1 (MQ=60)
                                                                                             GCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGA                                                         >  SRR19973069.607853/1‑150 (MQ=60)
                                                                                                ACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCA                                                      <  SRR19973069.2493425/150‑1 (MQ=60)
                                                                                                    CGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAAT                                                  <  SRR19973069.3629434/150‑1 (MQ=60)
                                                                                                      TGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGA                                                >  SRR19973069.2991448/1‑150 (MQ=60)
                                                                                                         GCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAAT                                             <  SRR19973069.1981506/150‑1 (MQ=60)
                                                                                                          CTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATC                                            <  SRR19973069.3221594/150‑1 (MQ=60)
                                                                                                           TGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATTACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCC                                           >  SRR19973069.3048178/1‑150 (MQ=60)
                                                                                                            GCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCC                                          <  SRR19973069.2515708/150‑1 (MQ=60)
                                                                                                              GCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCG                                        <  SRR19973069.3202/150‑1 (MQ=60)
                                                                                                               CTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGA                                       >  SRR19973069.2839273/1‑150 (MQ=60)
                                                                                                                TATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAA                                      >  SRR19973069.3300497/1‑150 (MQ=60)
                                                                                                                TATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAA                                      >  SRR19973069.1386593/1‑150 (MQ=60)
                                                                                                                   TGTAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAA                                   >  SRR19973069.444532/1‑150 (MQ=60)
                                                                                                                     TAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACT                                 >  SRR19973069.457434/1‑150 (MQ=60)
                                                                                                                     TAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACT                                 >  SRR19973069.457410/1‑150 (MQ=60)
                                                                                                                     TAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACT                                 <  SRR19973069.1176412/150‑1 (MQ=60)
                                                                                                                     TAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACT                                 <  SRR19973069.2307835/150‑1 (MQ=60)
                                                                                                                     TAAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACT                                 >  SRR19973069.2386382/1‑150 (MQ=60)
                                                                                                                      AAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTG                                <  SRR19973069.3270280/150‑1 (MQ=60)
                                                                                                                      AAAGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTG                                <  SRR19973069.3271088/150‑1 (MQ=60)
                                                                                                                        AGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCC                              <  SRR19973069.880353/150‑1 (MQ=60)
                                                                                                                        AGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCC                              >  SRR19973069.2955565/1‑150 (MQ=60)
                                                                                                                        AGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCC                              <  SRR19973069.649697/150‑1 (MQ=60)
                                                                                                                        AGAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCC                              <  SRR19973069.442324/150‑1 (MQ=60)
                                                                                                                         GAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCG                             <  SRR19973069.1702400/150‑1 (MQ=60)
                                                                                                                         GAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCG                             <  SRR19973069.1525097/150‑1 (MQ=60)
                                                                                                                         GAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCG                             <  SRR19973069.1566180/150‑1 (MQ=60)
                                                                                                                         GAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCG                             <  SRR19973069.1700963/150‑1 (MQ=60)
                                                                                                                         GAGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCG                             <  SRR19973069.3435890/150‑1 (MQ=60)
                                                                                                                          AGATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGC                            <  SRR19973069.556113/150‑1 (MQ=60)
                                                                                                                           GATCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAAGAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCT                           <  SRR19973069.2854291/150‑1 (MQ=60)
                                                                                                                             TCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGC                         >  SRR19973069.2311921/1‑150 (MQ=60)
                                                                                                                             TCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGC                         >  SRR19973069.3151056/1‑150 (MQ=60)
                                                                                                                             TCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGC                         >  SRR19973069.1234851/1‑150 (MQ=60)
                                                                                                                             TCCCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGC                         >  SRR19973069.3151414/1‑150 (MQ=60)
                                                                                                                               CCTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTG                       >  SRR19973069.539316/1‑150 (MQ=60)
                                                                                                                                CTGCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGC                      <  SRR19973069.1267425/150‑1 (MQ=60)
                                                                                                                                 TGCCGTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACATAACGTTTTGAACAGCAGGGAAAAACGGCAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCC                     <  SRR19973069.120872/150‑1 (MQ=60)
                                                                                                                                  GCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTTGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCG                    <  SRR19973069.41804/150‑1 (MQ=60)
                                                                                                                                  GCCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCG                    <  SRR19973069.41859/150‑1 (MQ=60)
                                                                                                                                   CCCTGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGA                   >  SRR19973069.523251/1‑150 (MQ=60)
                                                                                                                                      TGGATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAG                >  SRR19973069.3474135/1‑150 (MQ=60)
                                                                                                                                         ATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGT             <  SRR19973069.386504/150‑1 (MQ=60)
                                                                                                                                         ATCCGCAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGT             <  SRR19973069.386491/150‑1 (MQ=60)
                                                                                                                                              CAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCGT        >  SRR19973069.2911348/1‑150 (MQ=60)
                                                                                                                                              CAACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCGT        >  SRR19973069.178834/1‑150 (MQ=60)
                                                                                                                                               AACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCGTT       >  SRR19973069.263216/1‑150 (MQ=60)
                                                                                                                                               AACTGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCGTT       >  SRR19973069.263219/1‑150 (MQ=60)
                                                                                                                                                  TGTTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCGTTACT    <  SRR19973069.1201092/150‑1 (MQ=60)
                                                                                                                                                    TTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCGTTACTGG  <  SRR19973069.830984/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
AATTTGCTTTACATCTCCCGTAAACACTTTTCTGCGATACAATGCCTTTACGTTATGTAACGGAGAGTTCGGCATGTCACACCCCGCGTTAACGCAACTGCGTGCGCTGCGCTATTGTAAAGAGATCCCTGCCCTGGATCCGCAACTGCTCGACTGGCTGTTGCTGGAGGATTCCATGACAAAACGTTTTGAACAGCAGGGAAAAACGGTAAGCGTGACGATGATCCGCGAAGGGTTTGTCGAGCAGAATGAAATCCCCGAAGAACTGCCGCTGCTGCCGAAAGAGTCTCGTTACTGG  >  CP009273/4242361‑4242658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑