Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,846,112 C→A 100% I206I (ATC→ATA)  hypD → hydrogenase maturation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,846,1120CA99.0% 638.0 / ‑2.0 200I206I (ATC→ATA) hypDhydrogenase maturation protein
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (80/118);  total (81/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 202 total aligned reads displayed.

TGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATCGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAGTAT  >  CP009273/2845967‑2846256
                                                                                                                                                 |                                                                                                                                                
tGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAgg                                                                                                                                                <  1:2418898/148‑1 (MQ=255)
     cAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCg                                                                                                                                           <  1:647791/148‑1 (MQ=255)
        gCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACg                                                                                                                                        >  1:1984227/1‑148 (MQ=255)
          gAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGcc                                                                                                                                      <  1:1141596/148‑1 (MQ=255)
           aaaGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCt                                                                                                                                     >  2:252817/1‑148 (MQ=255)
           aaaGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCt                                                                                                                                     <  2:828206/148‑1 (MQ=255)
            aaGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCta                                                                                                                                    >  1:1361503/1‑148 (MQ=255)
              gcgcGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCtata                                                                                                                                  >  2:2189684/1‑148 (MQ=255)
                 cgTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAAtt                                                                                                                               >  2:263958/1‑148 (MQ=255)
                 cgTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAAtt                                                                                                                               <  1:41356/148‑1 (MQ=255)
                  gTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAAttt                                                                                                                              <  1:2805257/148‑1 (MQ=255)
                  gTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAAttt                                                                                                                              <  1:3072873/148‑1 (MQ=255)
                   tGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATCGGCACCGACGCCTATAAtttt                                                                                                                             <  2:535636/148‑1 (MQ=255)
                     aTGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTAt                                                                                                                           >  2:1869487/1‑148 (MQ=255)
                      tgtgCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATc                                                                                                                          >  1:2338709/1‑148 (MQ=255)
                      tgtgCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATc                                                                                                                          <  2:1360385/148‑1 (MQ=255)
                       gtgCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCg                                                                                                                         <  1:804940/148‑1 (MQ=255)
                       gtgCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCg                                                                                                                         >  2:1753174/1‑148 (MQ=255)
                        tgCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGc                                                                                                                        <  1:3465720/148‑1 (MQ=255)
                        tgCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGc                                                                                                                        <  1:1728724/148‑1 (MQ=255)
                         gCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGcc                                                                                                                       >  2:2669153/1‑148 (MQ=255)
                         gCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGcc                                                                                                                       <  2:666241/148‑1 (MQ=255)
                            gAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGc                                                                                                                    >  1:1051837/1‑148 (MQ=255)
                                ttttACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGAtt                                                                                                                <  1:2254640/148‑1 (MQ=255)
                                ttttACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGAtt                                                                                                                <  2:213672/148‑1 (MQ=255)
                                ttttACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGAtt                                                                                                                >  1:2205085/1‑148 (MQ=255)
                                 tttACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGAttt                                                                                                               >  1:1910996/1‑148 (MQ=255)
                                   tACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTc                                                                                                             <  2:2921471/148‑1 (MQ=255)
                                   tACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTc                                                                                                             <  2:73056/148‑1 (MQ=255)
                                   tACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGAAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTc                                                                                                             <  1:2038379/148‑1 (MQ=255)
                                   tACTTCGTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTc                                                                                                             <  2:2088925/148‑1 (MQ=255)
                                    aCTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCa                                                                                                            >  2:1717421/1‑148 (MQ=255)
                                    aCTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCa                                                                                                            <  2:445350/148‑1 (MQ=255)
                                    aCTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCa                                                                                                            <  2:2575751/148‑1 (MQ=255)
                                     cttcttCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCAt                                                                                                           <  1:1706988/148‑1 (MQ=255)
                                     cttcttCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCAt                                                                                                           <  2:2552155/148‑1 (MQ=255)
                                     cttcttCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCAt                                                                                                           <  1:2429322/148‑1 (MQ=255)
                                     cttcttCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCAt                                                                                                           <  1:1479008/148‑1 (MQ=255)
                                       tcttctGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCg                                                                                                         >  1:2374771/1‑148 (MQ=255)
                                             gCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCt                                                                                                   <  2:639119/148‑1 (MQ=255)
                                                 gCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCtggtg                                                                                               <  1:2389682/148‑1 (MQ=255)
                                                 gCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCtggtg                                                                                               >  1:190350/1‑148 (MQ=255)
                                                  cacaTTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCtggtgg                                                                                              <  1:2717763/148‑1 (MQ=255)
                                                  cacaTTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCtggtgg                                                                                              <  2:3294913/148‑1 (MQ=255)
                                                    caTTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGtt                                                                                            <  1:1520794/148‑1 (MQ=255)
                                                     aTTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATCATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTg                                                                                           <  1:3337849/148‑1 (MQ=255)
                                                     aTTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTg                                                                                           >  2:1986035/1‑148 (MQ=255)
                                                         cGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTgg                                                                                       >  1:2930105/1‑148 (MQ=255)
                                                           cTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGAt                                                                                     <  2:2970314/148‑1 (MQ=255)
                                                            ttATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATATGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGAtt                                                                                    <  1:3145962/148‑1 (MQ=255)
                                                            ttATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGAtt                                                                                    <  1:699907/148‑1 (MQ=255)
                                                            ttATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGAtt                                                                                    >  2:1460217/1‑148 (MQ=255)
                                                             tATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCGATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTc                                                                                   >  2:3036911/1‑148 (MQ=255)
                                                              aTCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCg                                                                                  >  1:3369900/1‑148 (MQ=255)
                                                               tCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGa                                                                                 >  2:2232656/1‑148 (MQ=255)
                                                                cccGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGaa                                                                                <  1:16983/148‑1 (MQ=255)
                                                                  cGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAAcc                                                                              >  2:23216/1‑148 (MQ=255)
                                                                  cGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAAcc                                                                              >  1:3329393/1‑148 (MQ=255)
                                                                       ttGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTg                                                                         >  2:2153994/1‑148 (MQ=255)
                                                                       ttGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTg                                                                         >  2:173076/1‑148 (MQ=255)
                                                                        tGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGa                                                                        <  2:1559041/148‑1 (MQ=255)
                                                                          cgcAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATc                                                                      >  2:258344/1‑148 (MQ=255)
                                                                           gcAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATct                                                                     <  1:53033/148‑1 (MQ=255)
                                                                              gTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTCTAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATctact                                                                  >  2:544149/1‑148 (MQ=255)
                                                                              gTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATctact                                                                  >  2:544133/1‑148 (MQ=255)
                                                                               tttGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATctacta                                                                 >  2:284120/1‑148 (MQ=255)
                                                                                ttGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCGATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCtactac                                                                >  2:3211412/1‑148 (MQ=255)
                                                                                ttGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGGATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCtactac                                                                >  2:867112/1‑148 (MQ=255)
                                                                                 tGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACa                                                               <  2:1205468/148‑1 (MQ=255)
                                                                                  gCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACaa                                                              <  2:2359757/148‑1 (MQ=255)
                                                                                      gAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCg                                                          <  2:1983994/148‑1 (MQ=255)
                                                                                            ccGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCa                                                    <  1:777645/148‑1 (MQ=255)
                                                                                            ccGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCa                                                    <  1:2422399/148‑1 (MQ=255)
                                                                                              ggATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATg                                                  <  2:2962884/148‑1 (MQ=255)
                                                                                                 tAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTg                                               <  1:550632/148‑1 (MQ=255)
                                                                                                  aaCGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTgg                                              <  2:3343217/148‑1 (MQ=255)
                                                                                                     ggTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTgc                                           <  1:850246/148‑1 (MQ=255)
                                                                                                       tATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTgcag                                         >  1:1723219/1‑148 (MQ=255)
                                                                                                        aTCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTgcagc                                        >  1:1128569/1‑148 (MQ=255)
                                                                                                         tCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTgcagca                                       >  2:1303238/1‑148 (MQ=255)
                                                                                                          cGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGcagcag                                      >  1:84428/1‑148 (MQ=255)
                                                                                                              gCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGaaaa                                  <  2:3123850/148‑1 (MQ=255)
                                                                                                               cGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAAt                                 <  2:1291214/148‑1 (MQ=255)
                                                                                                                gTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATa                                <  1:2494562/148‑1 (MQ=255)
                                                                                                                  tCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGc                              >  1:1404533/1‑148 (MQ=255)
                                                                                                                   ccTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCg                             <  1:1795285/148‑1 (MQ=255)
                                                                                                                   ccTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCg                             >  1:1819175/1‑148 (MQ=255)
                                                                                                                      cgcgCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATATTTTTATCGCCAGCGTTTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGcc                          <  1:3002262/148‑1 (MQ=255)
                                                                                                                              gTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCaa                  >  1:1480738/1‑148 (MQ=255)
                                                                                                                               tCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAg                 <  1:2596518/148‑1 (MQ=255)
                                                                                                                                  cGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTa              >  2:932039/1‑148 (MQ=255)
                                                                                                                                       gTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGaa         <  1:3316599/148‑1 (MQ=255)
                                                                                                                                         aTGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATc       >  1:3113059/1‑148 (MQ=255)
                                                                                                                                          tGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCa      <  2:244593/148‑1 (MQ=255)
                                                                                                                                          tGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCa      <  2:1621325/148‑1 (MQ=255)
                                                                                                                                           ggTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAg     <  2:1530042/148‑1 (MQ=255)
                                                                                                                                           ggTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAg     <  2:1530063/148‑1 (MQ=255)
                                                                                                                                            gTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAGt    <  2:859752/148‑1 (MQ=255)
                                                                                                                                             ttATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAGTa   <  2:3437091/148‑1 (MQ=255)
                                                                                                                                             ttATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAGTa   >  1:589967/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATCGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAGTAT  >  CP009273/2845967‑2846256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACTCTGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATCGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAGTATCGTC  >  CP009273/2845963‑2846260
                                                                                                                                                     |                                                                                                                                                    
ACTCTGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATA                                                                                                                                                      >  SRR19973065.1355643/1‑150 (MQ=60)
ACTCTGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATA                                                                                                                                                      <  SRR19973065.2113003/150‑1 (MQ=60)
    TGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCA                                                                                                                                                  <  SRR19973065.2434155/150‑1 (MQ=60)
    TGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCA                                                                                                                                                  <  SRR19973065.2434140/150‑1 (MQ=60)
       AACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCG                                                                                                                                               >  SRR19973065.923318/1‑150 (MQ=60)
         CAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGAC                                                                                                                                             <  SRR19973065.652052/150‑1 (MQ=60)
         CAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGAC                                                                                                                                             <  SRR19973065.2425052/150‑1 (MQ=60)
         CAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGAC                                                                                                                                             <  SRR19973065.1489617/150‑1 (MQ=60)
          AGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACG                                                                                                                                            >  SRR19973065.1997110/1‑150 (MQ=60)
          AGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACG                                                                                                                                            >  SRR19973065.1996877/1‑150 (MQ=60)
             CGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCT                                                                                                                                         >  SRR19973065.1093212/1‑150 (MQ=60)
             CGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCT                                                                                                                                         <  SRR19973065.2517325/150‑1 (MQ=60)
              GAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTA                                                                                                                                        >  SRR19973065.1370518/1‑150 (MQ=60)
              GAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTA                                                                                                                                        <  SRR19973065.1161926/150‑1 (MQ=60)
              GAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTA                                                                                                                                        <  SRR19973065.1148973/150‑1 (MQ=60)
               AAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTAT                                                                                                                                       >  SRR19973065.3016994/1‑150 (MQ=60)
                     CGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTT                                                                                                                                 <  SRR19973065.41856/150‑1 (MQ=60)
                     CGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTT                                                                                                                                 <  SRR19973065.3214616/150‑1 (MQ=60)
                      GTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTA                                                                                                                                <  SRR19973065.3092107/150‑1 (MQ=60)
                      GTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTA                                                                                                                                <  SRR19973065.2822622/150‑1 (MQ=60)
                      GTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTA                                                                                                                                <  SRR19973065.3091653/150‑1 (MQ=60)
                      GTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATACGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTA                                                                                                                                >  SRR19973065.1706306/1‑150 (MQ=60)
                        GATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATC                                                                                                                              <  SRR19973065.2102026/150‑1 (MQ=60)
                        GATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATC                                                                                                                              >  SRR19973065.2353312/1‑150 (MQ=60)
                         ATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCG                                                                                                                             <  SRR19973065.3149938/150‑1 (MQ=60)
                         ATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCG                                                                                                                             <  SRR19973065.228976/150‑1 (MQ=60)
                           GTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCC                                                                                                                           >  SRR19973065.226235/1‑150 (MQ=60)
                           GTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCC                                                                                                                           <  SRR19973065.810261/150‑1 (MQ=60)
                            TGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCA                                                                                                                          <  SRR19973065.3486826/150‑1 (MQ=60)
                            TGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCA                                                                                                                          <  SRR19973065.1740182/150‑1 (MQ=60)
                              CAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGC                                                                                                                        >  SRR19973065.1058806/1‑150 (MQ=60)
                                GAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGA                                                                                                                      >  SRR19973065.852741/1‑150 (MQ=60)
                                  ATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATT                                                                                                                    >  SRR19973065.2219193/1‑150 (MQ=60)
                                   TTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTT                                                                                                                   >  SRR19973065.1923547/1‑150 (MQ=60)
                                    TTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTT                                                                                                                  <  SRR19973065.2268922/150‑1 (MQ=60)
                                       TACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGAAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCAT                                                                                                               <  SRR19973065.2051439/150‑1 (MQ=60)
                                         CTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCG                                                                                                             >  SRR19973065.2389594/1‑150 (MQ=60)
                                         CTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCG                                                                                                             <  SRR19973065.2443818/150‑1 (MQ=60)
                                         CTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCG                                                                                                             <  SRR19973065.2444654/150‑1 (MQ=60)
                                         CTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCG                                                                                                             <  SRR19973065.1488432/150‑1 (MQ=60)
                                         CTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCG                                                                                                             <  SRR19973065.1718527/150‑1 (MQ=60)
                                         CTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCG                                                                                                             <  SRR19973065.1488460/150‑1 (MQ=60)
                                         CTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCG                                                                                                             <  SRR19973065.1718349/150‑1 (MQ=60)
                                                   CAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTG                                                                                                   >  SRR19973065.191838/1‑150 (MQ=60)
                                                     GCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGT                                                                                                 <  SRR19973065.2404678/150‑1 (MQ=60)
                                                     GCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGT                                                                                                 <  SRR19973065.2404568/150‑1 (MQ=60)
                                                      CACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTT                                                                                                <  SRR19973065.2734669/150‑1 (MQ=60)
                                                      CACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTT                                                                                                <  SRR19973065.525230/150‑1 (MQ=60)
                                                        CATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGC                                                                                              <  SRR19973065.1530760/150‑1 (MQ=60)
                                                         cctacgctgagCCCGACGTTGCGCAGTTGGCTGGAACAGCCGGAGACCGGTATCGAGGCGGTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATCATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCT                                                                                             <  SRR19973065.3358537/139‑1 (MQ=60)
                                                         ATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATCATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCT                                                                                             <  SRR19973065.3358234/150‑1 (MQ=60)
                                                           TACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGG                                                                                           >  SRR19973065.2947889/1‑150 (MQ=60)
                                                               CTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTC                                                                                       <  SRR19973065.1715169/150‑1 (MQ=60)
                                                               CTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTC                                                                                       <  SRR19973065.246922/150‑1 (MQ=60)
                                                               CTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTC                                                                                       >  SRR19973065.2842964/1‑150 (MQ=60)
                                                                TTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATATGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCG                                                                                      <  SRR19973065.3165070/150‑1 (MQ=60)
                                                                TTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCG                                                                                      <  SRR19973065.704566/150‑1 (MQ=60)
                                                                TTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCG                                                                                      <  SRR19973065.704616/150‑1 (MQ=60)
                                                                TTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCG                                                                                      >  SRR19973065.3390512/1‑150 (MQ=60)
                                                                TTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCG                                                                                      <  SRR19973065.1408703/150‑1 (MQ=60)
                                                                    CCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACC                                                                                  <  SRR19973065.833075/150‑1 (MQ=60)
                                                                    CCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACC                                                                                  >  SRR19973065.3349723/1‑150 (MQ=60)
                                                                    CCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACC                                                                                  >  SRR19973065.3349710/1‑150 (MQ=60)
                                                                    CCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACC                                                                                  >  SRR19973065.3349703/1‑150 (MQ=60)
                                                                    CCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACC                                                                                  <  SRR19973065.17226/150‑1 (MQ=60)
                                                                             GCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCT                                                                         <  SRR19973065.396315/150‑1 (MQ=60)
                                                                              CGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTA                                                                        >  SRR19973065.1527277/1‑150 (MQ=60)
                                                                              CGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTA                                                                        >  SRR19973065.3458059/1‑150 (MQ=60)
                                                                               GCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTAC                                                                       <  SRR19973065.54469/150‑1 (MQ=60)
                                                                               GCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTAC                                                                       <  SRR19973065.53617/150‑1 (MQ=60)
                                                                                     TGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAG                                                                 <  SRR19973065.2360517/150‑1 (MQ=60)
                                                                                           AACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGG                                                           >  SRR19973065.1916777/1‑150 (MQ=60)
                                                                                                CCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATG                                                      <  SRR19973065.665110/150‑1 (MQ=60)
                                                                                                CCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATG                                                      <  SRR19973065.782812/150‑1 (MQ=60)
                                                                                                CCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATG                                                      <  SRR19973065.2437678/150‑1 (MQ=60)
                                                                                                 CGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGC                                                     >  SRR19973065.2049425/1‑150 (MQ=60)
                                                                                                    ATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGG                                                  >  SRR19973065.1766615/1‑150 (MQ=60)
                                                                                                     TAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGT                                                 <  SRR19973065.554173/150‑1 (MQ=60)
                                                                                                         GGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAG                                             <  SRR19973065.855753/150‑1 (MQ=60)
                                                                                                         GGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAG                                             >  SRR19973065.1734659/1‑150 (MQ=60)
                                                                                                         GGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAG                                             >  SRR19973065.1734963/1‑150 (MQ=60)
                                                                                                          GTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGC                                            >  SRR19973065.1135908/1‑150 (MQ=60)
                                                                                                          GTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGC                                            >  SRR19973065.1135922/1‑150 (MQ=60)
                                                                                                            ATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAG                                          >  SRR19973065.85253/1‑150 (MQ=60)
                                                                                                                    GTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGC                                  <  SRR19973065.2510258/150‑1 (MQ=60)
                                                                                                                    GTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGC                                  >  SRR19973065.1413737/1‑150 (MQ=60)
                                                                                                                     TTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCG                                 >  SRR19973065.1830064/1‑150 (MQ=60)
                                                                                                                     TTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCG                                 >  SRR19973065.1831156/1‑150 (MQ=60)
                                                                                                                       CCTCGCGCCGGGTCACGTCAGTATGTTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGC                               <  SRR19973065.1840292/150‑1 (MQ=60)
                                                                                                                       CCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGC                               <  SRR19973065.1807067/150‑1 (MQ=60)
                                                                                                                          CGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATATTTTTATCGCCAGCGTTTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCA                            <  SRR19973065.3020655/150‑1 (MQ=60)
                                                                                                                                GGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAA                      >  SRR19973065.1490197/1‑150 (MQ=60)
                                                                                                                                 GGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAG                     <  SRR19973065.2648750/150‑1 (MQ=60)
                                                                                                                                  GTCACGTCAGTATGGTTATAGGCACCGACGCCTTTAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGG                    <  SRR19973065.3090425/150‑1 (MQ=60)
                                                                                                                                   TCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGT                   <  SRR19973065.2612657/150‑1 (MQ=60)
                                                                                                                                       GTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAG               >  SRR19973065.2736314/1‑150 (MQ=60)
                                                                                                                                        TCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGA              <  SRR19973065.796388/150‑1 (MQ=60)
                                                                                                                                           GTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATC           <  SRR19973065.3336825/150‑1 (MQ=60)
                                                                                                                                           GTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATC           >  SRR19973065.3132033/1‑150 (MQ=60)
                                                                                                                                               GGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAGTA       >  SRR19973065.593810/1‑150 (MQ=60)
                                                                                                                                               GGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAGTA       >  SRR19973065.593625/1‑150 (MQ=60)
                                                                                                                                               GGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAGTA       >  SRR19973065.593620/1‑150 (MQ=60)
                                                                                                                                                    TAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAGTATCGTC  <  SRR19973065.1764712/150‑1 (MQ=60)
                                                                                                                                                    TAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAGTATCGTC  >  SRR19973065.24010/1‑150 (MQ=60)
                                                                                                                                                    TAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAGTATCGTC  >  SRR19973065.2675980/1‑150 (MQ=60)
                                                                                                                                                    TAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAGTATCGTC  >  SRR19973065.1318506/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
ACTCTGCAACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATCGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAGTATCGTC  >  CP009273/2845963‑2846260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑