Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,084,103 A→T 94.8% L404* (TTA→TAA)  pgaB ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,084,1030AT94.8% 790.9 / 21.1 231L404* (TTA→TAA) pgaBpoly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (7/5);  new base T (105/114);  total (112/119)
Fisher's exact test for biased strand distribution p-value = 5.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 232 total aligned reads displayed.

AGATCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCAAAT  >  CP009273/1083956‑1084249
                                                                                                                                                   |                                                                                                                                                  
aGATCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTAt                                                                                                                                                  >  1:1985676/1‑150 (MQ=255)
 gATCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTtata                                                                                                                                                 <  1:3219712/150‑1 (MQ=255)
  aTCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATAc                                                                                                                                                >  1:2542597/1‑150 (MQ=255)
     ttCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCgg                                                                                                                                             >  2:2564236/1‑150 (MQ=255)
       cATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCa                                                                                                                                           >  2:938215/1‑150 (MQ=255)
       cATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCa                                                                                                                                           <  2:448698/150‑1 (MQ=255)
          ataACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATcc                                                                                                                                        <  2:267549/150‑1 (MQ=255)
          ataACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATAACGGCATcc                                                                                                                                        >  2:3680969/1‑150 (MQ=255)
           taACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCa                                                                                                                                       >  2:2407226/1‑150 (MQ=255)
            aaCATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCAc                                                                                                                                      >  2:4109946/1‑150 (MQ=255)
             aCATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACg                                                                                                                                     <  2:660437/150‑1 (MQ=255)
             aCATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACg                                                                                                                                     <  2:2973506/150‑1 (MQ=255)
             aCATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACg                                                                                                                                     <  2:4018360/150‑1 (MQ=255)
                tGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAACTCCCAGCTTTATACCGGCATCCACGCAt                                                                                                                                  <  2:2854962/150‑1 (MQ=255)
                tGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCAt                                                                                                                                  <  1:1836866/150‑1 (MQ=255)
                  ccAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAg                                                                                                                                <  2:697219/150‑1 (MQ=255)
                   cAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGa                                                                                                                               >  1:3012386/1‑150 (MQ=255)
                    aaCTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGAt                                                                                                                              <  2:1967994/150‑1 (MQ=255)
                            ctctGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTacac                                                                                                                      <  1:1258949/150‑1 (MQ=255)
                                gACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGa                                                                                                                  <  2:1816890/150‑1 (MQ=255)
                                gACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGa                                                                                                                  <  1:274157/150‑1 (MQ=255)
                                 aCTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAg                                                                                                                 <  1:1093083/150‑1 (MQ=255)
                                     tGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCggg                                                                                                             >  2:334618/1‑150 (MQ=255)
                                     tGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCggg                                                                                                             <  1:3362548/150‑1 (MQ=255)
                                       tCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTa                                                                                                           >  2:1209419/1‑150 (MQ=255)
                                        cATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTAc                                                                                                          <  2:3610595/150‑1 (MQ=255)
                                         aTCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACg                                                                                                         <  1:1735910/150‑1 (MQ=255)
                                          tCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGt                                                                                                        >  1:1670137/1‑150 (MQ=255)
                                          tCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGt                                                                                                        >  2:4169962/1‑150 (MQ=255)
                                            gAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTaa                                                                                                      >  1:1083910/1‑150 (MQ=255)
                                              aaGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAAtt                                                                                                    <  1:208096/150‑1 (MQ=255)
                                               aGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTg                                                                                                   <  1:1820034/150‑1 (MQ=255)
                                               aGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTg                                                                                                   <  1:2912374/150‑1 (MQ=255)
                                                 gagagagaCGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGcc                                                                                                 <  2:3053161/150‑1 (MQ=255)
                                                   gagagaCGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAg                                                                                               <  2:2264438/150‑1 (MQ=255)
                                                   gagagaCGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAg                                                                                               <  2:4308078/150‑1 (MQ=255)
                                                     gagaCGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGc                                                                                             >  1:31889/1‑150 (MQ=255)
                                                      agaCGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCa                                                                                            >  1:4008468/1‑150 (MQ=255)
                                                        aCGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAAc                                                                                          <  2:3281860/150‑1 (MQ=255)
                                                         cGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAAcc                                                                                         >  2:3175496/1‑150 (MQ=255)
                                                          ggTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAAccc                                                                                        <  1:3661697/150‑1 (MQ=255)
                                                              atatTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACt                                                                                    <  2:2037048/150‑1 (MQ=255)
                                                               tatTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTa                                                                                   <  2:2851626/150‑1 (MQ=255)
                                                               tatTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTa                                                                                   >  1:3731377/1‑150 (MQ=255)
                                                                  tGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTaaaa                                                                                >  1:686410/1‑150 (MQ=255)
                                                                    ttCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAat                                                                              <  1:2477096/150‑1 (MQ=255)
                                                                     tCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTCCGTAATTGCCAGGCAACCCGACTAAAAata                                                                             >  2:2369951/1‑150 (MQ=255)
                                                                     tCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAata                                                                             >  1:2231545/1‑150 (MQ=255)
                                                                     tCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAata                                                                             >  1:2231640/1‑150 (MQ=255)
                                                                       aGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATc                                                                           >  1:1464047/1‑150 (MQ=255)
                                                                       aGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATc                                                                           >  2:923167/1‑150 (MQ=255)
                                                                        ggATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCt                                                                          >  1:24072/1‑150 (MQ=255)
                                                                         gATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTg                                                                         <  1:2509393/150‑1 (MQ=255)
                                                                          aTGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGc                                                                        <  2:1050490/150‑1 (MQ=255)
                                                                              aTTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTc                                                                    <  1:2727214/150‑1 (MQ=255)
                                                                              aTTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTc                                                                    >  1:1699628/1‑150 (MQ=255)
                                                                              aTTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTGTc                                                                    >  2:325914/1‑150 (MQ=255)
                                                                                ttGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCAt                                                                  <  1:646840/150‑1 (MQ=255)
                                                                                 tgtgCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCAtt                                                                 <  2:54147/150‑1 (MQ=255)
                                                                                  gtgCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTg                                                                <  1:287062/150‑1 (MQ=255)
                                                                                      ttttttCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAg                                                            >  2:3235182/1‑150 (MQ=255)
                                                                                         tttCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCaa                                                         <  1:3667568/150‑1 (MQ=255)
                                                                                           tctcCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAAc                                                       <  1:3614245/150‑1 (MQ=255)
                                                                                                ccTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGAttt                                                  <  2:1308716/150‑1 (MQ=255)
                                                                                                ccTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGAttt                                                  >  2:3878388/1‑150 (MQ=255)
                                                                                                 cTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTg                                                 <  2:3731078/150‑1 (MQ=255)
                                                                                                  tGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTgg                                                <  1:3381318/150‑1 (MQ=255)
                                                                                                    ttGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGaa                                              >  2:680949/1‑150 (MQ=255)
                                                                                                    ttGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGaa                                              >  2:770358/1‑150 (MQ=255)
                                                                                                        tAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCa                                          >  1:2494518/1‑150 (MQ=255)
                                                                                                        tAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCa                                          <  1:4435898/150‑1 (MQ=255)
                                                                                                        tAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCa                                          >  1:2208264/1‑150 (MQ=255)
                                                                                                          aGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGa                                        >  1:1521563/1‑150 (MQ=255)
                                                                                                          aGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGa                                        <  1:4606561/150‑1 (MQ=255)
                                                                                                             aTTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACct                                     >  2:3470880/1‑150 (MQ=255)
                                                                                                              ttttACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACctc                                    >  1:384090/1‑150 (MQ=255)
                                                                                                                ttACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCtt                                  <  1:4538965/150‑1 (MQ=255)
                                                                                                                ttACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCtt                                  <  2:3579168/150‑1 (MQ=255)
                                                                                                                  aCTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTg                                <  1:4222596/150‑1 (MQ=255)
                                                                                                                    tCGCGTTAATGTGGGTTCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGAc                              >  2:1990127/1‑150 (MQ=255)
                                                                                                                    tCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGAc                              <  2:3079969/150‑1 (MQ=255)
                                                                                                                          tAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGccc                        >  1:2581576/1‑150 (MQ=255)
                                                                                                                          tAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGccc                        >  1:889884/1‑150 (MQ=255)
                                                                                                                          tAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGccc                        >  1:2062321/1‑150 (MQ=255)
                                                                                                                              gtgGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCa                    <  2:4279179/150‑1 (MQ=255)
                                                                                                                                gggATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGTCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCAcc                  >  1:97217/1‑150 (MQ=255)
                                                                                                                                  gATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCAt                <  2:4590732/150‑1 (MQ=255)
                                                                                                                                   aTCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATc               <  1:758931/150‑1 (MQ=255)
                                                                                                                                   aTCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATc               <  2:1369578/150‑1 (MQ=255)
                                                                                                                                     cTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCgg             >  1:4137090/1‑150 (MQ=255)
                                                                                                                                      tAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCggg            >  2:2197595/1‑150 (MQ=255)
                                                                                                                                      tAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCggg            >  1:1306269/1‑150 (MQ=255)
                                                                                                                                       aaaTCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGa           >  2:2612828/1‑150 (MQ=255)
                                                                                                                                       aaaTCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGa           >  1:1587423/1‑150 (MQ=255)
                                                                                                                                        aaTCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGAt          <  1:4169962/150‑1 (MQ=255)
                                                                                                                                        aaTCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCGTTGACCAGCCCATCACCATCGGGAt          >  2:2497801/1‑150 (MQ=255)
                                                                                                                                           cccAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAg       >  1:1483373/1‑150 (MQ=255)
                                                                                                                                            ccAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGc      <  1:3374868/150‑1 (MQ=255)
                                                                                                                                            ccAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGc      <  2:2714217/150‑1 (MQ=255)
                                                                                                                                                cTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCAAAt  <  1:606855/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGATCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCAAAT  >  CP009273/1083956‑1084249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAAGATCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCAAATGCC  >  CP009273/1083954‑1084252
                                                                                                                                                     |                                                                                                                                                     
CAAGATCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTT                                                                                                                                                       <  SRR19973073.4519771/150‑1 (MQ=60)
  AGATCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTAT                                                                                                                                                     >  SRR19973073.1989789/1‑150 (MQ=60)
   GATCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATA                                                                                                                                                    <  SRR19973073.3226205/150‑1 (MQ=60)
    ATCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATAC                                                                                                                                                   >  SRR19973073.2547847/1‑150 (MQ=60)
     TCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACC                                                                                                                                                  <  SRR19973073.2232275/150‑1 (MQ=60)
             TAACATGCCAACTTGTGCTCTGACTCTGTCATCGACAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCA                                                                                                                                          <  SRR19973073.1526676/150‑1 (MQ=60)
                  TGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCAT                                                                                                                                     >  SRR19973073.413515/1‑150 (MQ=60)
                  TGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCAT                                                                                                                                     <  SRR19973073.1840650/150‑1 (MQ=60)
                    CCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAG                                                                                                                                   >  SRR19973073.4603093/1‑150 (MQ=60)
                    CCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAG                                                                                                                                   <  SRR19973073.1421866/150‑1 (MQ=60)
                     CAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGA                                                                                                                                  >  SRR19973073.3018491/1‑150 (MQ=60)
                      AACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGAT                                                                                                                                 <  SRR19973073.3642556/150‑1 (MQ=60)
                              CTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACAC                                                                                                                         <  SRR19973073.1261583/150‑1 (MQ=60)
                                  GACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGA                                                                                                                     <  SRR19973073.274752/150‑1 (MQ=60)
                                   ACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAG                                                                                                                    <  SRR19973073.1095331/150‑1 (MQ=60)
                                    CTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGC                                                                                                                   >  SRR19973073.1884280/1‑150 (MQ=60)
                                    CTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGC                                                                                                                   >  SRR19973073.1484012/1‑150 (MQ=60)
                                       TGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGG                                                                                                                <  SRR19973073.3369328/150‑1 (MQ=60)
                                       TGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGG                                                                                                                <  SRR19973073.3369518/150‑1 (MQ=60)
                                          CATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTAC                                                                                                             <  SRR19973073.1828684/150‑1 (MQ=60)
                                          CATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTAC                                                                                                             <  SRR19973073.545510/150‑1 (MQ=60)
                                           ATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACG                                                                                                            <  SRR19973073.1739518/150‑1 (MQ=60)
                                            TCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGT                                                                                                           >  SRR19973073.1673606/1‑150 (MQ=60)
                                             CGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTA                                                                                                          >  SRR19973073.2252100/1‑150 (MQ=60)
                                              GAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAA                                                                                                         >  SRR19973073.1086163/1‑150 (MQ=60)
                                              GAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAA                                                                                                         >  SRR19973073.1086145/1‑150 (MQ=60)
                                               AAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAAT                                                                                                        <  SRR19973073.4415242/150‑1 (MQ=60)
                                                AAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATT                                                                                                       <  SRR19973073.208580/150‑1 (MQ=60)
                                                AAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATT                                                                                                       <  SRR19973073.1393448/150‑1 (MQ=60)
                                                 AGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTG                                                                                                      <  SRR19973073.1823797/150‑1 (MQ=60)
                                                 AGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTG                                                                                                      <  SRR19973073.2918326/150‑1 (MQ=60)
                                                    AGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCA                                                                                                   <  SRR19973073.4043382/150‑1 (MQ=60)
                                                    AGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCA                                                                                                   >  SRR19973073.3384344/1‑150 (MQ=60)
                                                       GAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGC                                                                                                >  SRR19973073.31982/1‑150 (MQ=60)
                                                        AGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCA                                                                                               >  SRR19973073.4016470/1‑150 (MQ=60)
                                                           CGGTGATATTGTTAAGGATGTATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGATTTATACCGTCATCCACGCATTGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACC                                                                                            <  SRR19973073.274441/150‑1 (MQ=60)
                                                            GGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCC                                                                                           <  SRR19973073.3669059/150‑1 (MQ=60)
                                                            GGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCC                                                                                           <  SRR19973073.3669044/150‑1 (MQ=60)
                                                                 TATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTA                                                                                      >  SRR19973073.3738870/1‑150 (MQ=60)
                                                                 TATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTA                                                                                      >  SRR19973073.2138525/1‑150 (MQ=60)
                                                                    TGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAA                                                                                   >  SRR19973073.687913/1‑150 (MQ=60)
                                                                    TGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAA                                                                                   >  SRR19973073.687838/1‑150 (MQ=60)
                                                                     GTTCAGGATGAATTTGTGCTTTTTTCTCCCNTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAA                                                                                  >  SRR19973073.4286889/1‑150 (MQ=60)
                                                                      TTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAAT                                                                                 <  SRR19973073.2482224/150‑1 (MQ=60)
                                                                      TTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAAT                                                                                 <  SRR19973073.2482409/150‑1 (MQ=60)
                                                                       TCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATA                                                                                >  SRR19973073.2236251/1‑150 (MQ=60)
                                                                       TCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATA                                                                                >  SRR19973073.2236156/1‑150 (MQ=60)
                                                                       TCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATA                                                                                >  SRR19973073.2236169/1‑150 (MQ=60)
                                                                         AGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATC                                                                              >  SRR19973073.1467113/1‑150 (MQ=60)
                                                                          GGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCT                                                                             >  SRR19973073.24146/1‑150 (MQ=60)
                                                                           GATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTG                                                                            <  SRR19973073.2514588/150‑1 (MQ=60)
                                                                             TGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATGCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCT                                                                          <  SRR19973073.1546010/150‑1 (MQ=60)
                                                                             TGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCT                                                                          <  SRR19973073.1294730/150‑1 (MQ=60)
                                                                                ATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTC                                                                       >  SRR19973073.625073/1‑150 (MQ=60)
                                                                                ATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTC                                                                       >  SRR19973073.1703625/1‑150 (MQ=60)
                                                                                ATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTC                                                                       <  SRR19973073.2732780/150‑1 (MQ=60)
                                                                                ATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTC                                                                       <  SRR19973073.2729186/150‑1 (MQ=60)
                                                                                ATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTC                                                                       <  SRR19973073.2729176/150‑1 (MQ=60)
                                                                                ATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTC                                                                       >  SRR19973073.1703156/1‑150 (MQ=60)
                                                                                  TTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCAT                                                                     <  SRR19973073.648221/150‑1 (MQ=60)
                                                                                  TTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCAT                                                                     <  SRR19973073.648208/150‑1 (MQ=60)
                                                                                  TTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCAT                                                                     >  SRR19973073.3271387/1‑150 (MQ=60)
                                                                                    GTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTG                                                                   >  SRR19973073.3675095/1‑150 (MQ=60)
                                                                                    GTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTG                                                                   <  SRR19973073.287682/150‑1 (MQ=60)
                                                                                     TGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGG                                                                  >  SRR19973073.3034066/1‑150 (MQ=60)
                                                                                       CTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTA                                                                <  SRR19973073.2904432/150‑1 (MQ=60)
                                                                                       CTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTA                                                                <  SRR19973073.2475778/150‑1 (MQ=60)
                                                                                           TTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAA                                                            <  SRR19973073.3674925/150‑1 (MQ=60)
                                                                                           TTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAA                                                            <  SRR19973073.2354185/150‑1 (MQ=60)
                                                                                             TCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAAC                                                          <  SRR19973073.3621500/150‑1 (MQ=60)
                                                                                               TCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGA                                                        >  SRR19973073.3335940/1‑150 (MQ=60)
                                                                                                CCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGAT                                                       <  SRR19973073.1879937/150‑1 (MQ=60)
                                                                                                CCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGAT                                                       <  SRR19973073.3786040/150‑1 (MQ=60)
                                                                                                   CTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTG                                                    <  SRR19973073.341679/150‑1 (MQ=60)
                                                                                                   CTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTG                                                    <  SRR19973073.341637/150‑1 (MQ=60)
                                                                                                    TGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGG                                                   <  SRR19973073.3388119/150‑1 (MQ=60)
                                                                                                         GTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACC                                              <  SRR19973073.1732543/150‑1 (MQ=60)
                                                                                                          TAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCA                                             <  SRR19973073.1232866/150‑1 (MQ=60)
                                                                                                          TAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCA                                             >  SRR19973073.2499672/1‑150 (MQ=60)
                                                                                                          TAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCA                                             <  SRR19973073.4445045/150‑1 (MQ=60)
                                                                                                          TAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCA                                             <  SRR19973073.4444808/150‑1 (MQ=60)
                                                                                                          TAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCA                                             >  SRR19973073.2499678/1‑150 (MQ=60)
                                                                                                          TAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCA                                             >  SRR19973073.2212816/1‑150 (MQ=60)
                                                                                                          TAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCA                                             >  SRR19973073.2212851/1‑150 (MQ=60)
                                                                                                           AAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAG                                            <  SRR19973073.2865529/150‑1 (MQ=60)
                                                                                                            AGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGA                                           >  SRR19973073.1524697/1‑150 (MQ=60)
                                                                                                            AGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGA                                           <  SRR19973073.731677/150‑1 (MQ=60)
                                                                                                            AGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGA                                           <  SRR19973073.4615755/150‑1 (MQ=60)
                                                                                                                TTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTC                                       >  SRR19973073.384850/1‑150 (MQ=60)
                                                                                                                  TTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTT                                     <  SRR19973073.4548065/150‑1 (MQ=60)
                                                                                                                  TTACTCGAGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTT                                     <  SRR19973073.1683674/150‑1 (MQ=60)
                                                                                                                    ACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTG                                   <  SRR19973073.4230736/150‑1 (MQ=60)
                                                                                                                    ACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTG                                   <  SRR19973073.4230970/150‑1 (MQ=60)
                                                                                                                    ACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTG                                   >  SRR19973073.3686559/1‑150 (MQ=60)
                                                                                                                        GCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCA                               >  SRR19973073.1659016/1‑150 (MQ=60)
                                                                                                                         CGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAG                              >  SRR19973073.2069741/1‑150 (MQ=60)
                                                                                                                          GTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGC                             >  SRR19973073.4616158/1‑150 (MQ=60)
                                                                                                                            TAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCC                           >  SRR19973073.2066556/1‑150 (MQ=60)
                                                                                                                            TAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCC                           >  SRR19973073.2586886/1‑150 (MQ=60)
                                                                                                                            TAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCC                           >  SRR19973073.2066617/1‑150 (MQ=60)
                                                                                                                            TAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCC                           >  SRR19973073.3217127/1‑150 (MQ=60)
                                                                                                                            TAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCC                           >  SRR19973073.891723/1‑150 (MQ=60)
                                                                                                                            TAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCC                           >  SRR19973073.891696/1‑150 (MQ=60)
                                                                                                                              ATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCAT                         >  SRR19973073.2562744/1‑150 (MQ=60)
                                                                                                                                  GGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGTCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACC                     >  SRR19973073.97458/1‑150 (MQ=60)
                                                                                                                                  GGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACC                     <  SRR19973073.2939409/150‑1 (MQ=60)
                                                                                                                                     ATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATC                  <  SRR19973073.1808227/150‑1 (MQ=60)
                                                                                                                                     ATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATC                  <  SRR19973073.932850/150‑1 (MQ=60)
                                                                                                                                     ATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATC                  <  SRR19973073.760459/150‑1 (MQ=60)
                                                                                                                                      TCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCG                 <  SRR19973073.1491995/150‑1 (MQ=60)
                                                                                                                                       CTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGG                >  SRR19973073.4145694/1‑150 (MQ=60)
                                                                                                                                       CTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGG                >  SRR19973073.4145365/1‑150 (MQ=60)
                                                                                                                                        TAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGG               >  SRR19973073.1309032/1‑150 (MQ=60)
                                                                                                                                        TAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGG               >  SRR19973073.2518985/1‑150 (MQ=60)
                                                                                                                                         AAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGA              >  SRR19973073.3919830/1‑150 (MQ=60)
                                                                                                                                         AAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGA              >  SRR19973073.2509856/1‑150 (MQ=60)
                                                                                                                                         AAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGA              >  SRR19973073.1590702/1‑150 (MQ=60)
                                                                                                                                          AATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGAT             <  SRR19973073.4178272/150‑1 (MQ=60)
                                                                                                                                             CCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAG          >  SRR19973073.1486465/1‑150 (MQ=60)
                                                                                                                                              CCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGC         <  SRR19973073.3381669/150‑1 (MQ=60)
                                                                                                                                                 GCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCAAA      >  SRR19973073.2942454/1‑150 (MQ=60)
                                                                                                                                                  CTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCAAAT     <  SRR19973073.608153/150‑1 (MQ=60)
                                                                                                                                                   TTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCAAATG    >  SRR19973073.631624/1‑150 (MQ=60)
                                                                                                                                                   TTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCAAATG    >  SRR19973073.1191660/1‑150 (MQ=60)
                                                                                                                                                   TTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCAAATG    >  SRR19973073.1224129/1‑150 (MQ=60)
                                                                                                                                                     TATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCAAATGCC  >  SRR19973073.2273743/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CAAGATCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCAAATGCC  >  CP009273/1083954‑1084252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑