Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,084,103 A→T 94.5% L404* (TTA→TAA)  pgaB ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,084,1030AT94.5% 683.2 / 21.3 201L404* (TTA→TAA) pgaBpoly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (4/7);  new base T (112/78);  total (116/85)
Fisher's exact test for biased strand distribution p-value = 2.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 201 total aligned reads displayed.

AGATCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCAAA  >  CP009273/1083956‑1084248
                                                                                                                                                   |                                                                                                                                                 
  aTCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTATTTTGGGATCTAAATCCCAGCTTTATAc                                                                                                                                               <  2:154992/150‑1 (MQ=255)
  aTCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATAc                                                                                                                                               <  1:64800/150‑1 (MQ=255)
  aTCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATAc                                                                                                                                               >  2:2871843/1‑150 (MQ=255)
          ataACCTGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATcc                                                                                                                                       <  1:3846670/150‑1 (MQ=255)
          ataACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATcc                                                                                                                                       >  1:3332206/1‑150 (MQ=255)
           taACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCa                                                                                                                                      >  1:3426785/1‑150 (MQ=255)
                tGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCAt                                                                                                                                 >  2:1304115/1‑150 (MQ=255)
                   cAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGa                                                                                                                              >  1:1256236/1‑150 (MQ=255)
                    aaCTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGAt                                                                                                                             >  1:3662447/1‑150 (MQ=255)
                    aaCTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGAt                                                                                                                             >  1:863129/1‑150 (MQ=255)
                       ttGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGtt                                                                                                                          <  2:1082851/150‑1 (MQ=255)
                          tgCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTac                                                                                                                       >  1:2507137/1‑150 (MQ=255)
                          tgCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTac                                                                                                                       <  1:2359446/150‑1 (MQ=255)
                          tgCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTac                                                                                                                       >  2:2056377/1‑150 (MQ=255)
                          tgCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCGGGTGGTAAGTATTTTACTCGCGTTAAGGTGGGATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTac                                                                                                                       >  2:2056400/1‑150 (MQ=255)
                              ctGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCt                                                                                                                   >  2:1563794/1‑150 (MQ=255)
                                 aCTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTTAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAg                                                                                                                <  2:2516963/150‑1 (MQ=255)
                                 aCTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAg                                                                                                                >  1:665511/1‑150 (MQ=255)
                                     tGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCggg                                                                                                            <  1:2609417/150‑1 (MQ=255)
                                      gTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGt                                                                                                           <  1:202243/150‑1 (MQ=255)
                                          tCGAAAGGAGAGAGACGTTGATTGTGTTCAGGAAGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCATCATTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGt                                                                                                       <  2:604566/150‑1 (MQ=255)
                                          tCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGt                                                                                                       <  2:3309105/150‑1 (MQ=255)
                                            gAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTaa                                                                                                     >  1:1307718/1‑150 (MQ=255)
                                             aaaGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAAt                                                                                                    >  2:2260979/1‑150 (MQ=255)
                                               aGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTg                                                                                                  >  1:989976/1‑150 (MQ=255)
                                               aGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTg                                                                                                  >  1:2752307/1‑150 (MQ=255)
                                                 gagagagaCGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGcc                                                                                                <  1:1123640/150‑1 (MQ=255)
                                                  agagagaCGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCa                                                                                               <  1:2271871/150‑1 (MQ=255)
                                                  agagagaCGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCa                                                                                               >  1:3349777/1‑150 (MQ=255)
                                                  agagagaCGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCa                                                                                               >  1:3349767/1‑150 (MQ=255)
                                                  agagagaCGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTAAACCTGAGCGGGTACGTAATTGCCa                                                                                               >  1:3349736/1‑150 (MQ=255)
                                                   gagagaCGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAg                                                                                              <  1:781215/150‑1 (MQ=255)
                                                   gagagaCGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAg                                                                                              >  2:886078/1‑150 (MQ=255)
                                                         cGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAAcc                                                                                        >  1:1574305/1‑150 (MQ=255)
                                                          ggTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAAccc                                                                                       >  2:4125210/1‑150 (MQ=255)
                                                           gTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCg                                                                                      <  1:1218044/150‑1 (MQ=255)
                                                              atatTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACt                                                                                   <  1:1398522/150‑1 (MQ=255)
                                                              atatTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACt                                                                                   <  1:1424346/150‑1 (MQ=255)
                                                               tatTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTa                                                                                  >  1:314085/1‑150 (MQ=255)
                                                               tatTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTa                                                                                  <  1:879790/150‑1 (MQ=255)
                                                                atTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTaa                                                                                 <  1:3235501/150‑1 (MQ=255)
                                                                    ttCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAat                                                                             >  2:2792796/1‑150 (MQ=255)
                                                                    ttCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAat                                                                             <  2:43364/150‑1 (MQ=255)
                                                                      cAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAatat                                                                           >  1:1730505/1‑150 (MQ=255)
                                                                       aGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATc                                                                          >  2:463797/1‑150 (MQ=255)
                                                                        ggATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTACATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCt                                                                         <  2:1716840/150‑1 (MQ=255)
                                                                        ggATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCt                                                                         <  1:1645430/150‑1 (MQ=255)
                                                                         gATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTg                                                                        >  1:3656686/1‑150 (MQ=255)
                                                                            gAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCtt                                                                     >  2:2574495/1‑150 (MQ=255)
                                                                             aaTTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCttt                                                                    <  1:1066937/150‑1 (MQ=255)
                                                                             aaTTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCttt                                                                    >  2:3913811/1‑150 (MQ=255)
                                                                                ttGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCAt                                                                 <  2:4000423/150‑1 (MQ=255)
                                                                                ttGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCAt                                                                 <  1:1822768/150‑1 (MQ=255)
                                                                                ttGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCAt                                                                 <  1:2435317/150‑1 (MQ=255)
                                                                                     cTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTa                                                            >  1:1016613/1‑150 (MQ=255)
                                                                                      ttttttCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAg                                                           >  1:2989725/1‑150 (MQ=255)
                                                                                        ttttCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCa                                                         >  1:2662034/1‑150 (MQ=255)
                                                                                         tttCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCaa                                                        <  1:825368/150‑1 (MQ=255)
                                                                                         tttCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCaa                                                        <  1:2243043/150‑1 (MQ=255)
                                                                                          ttCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCaaa                                                       >  2:929772/1‑150 (MQ=255)
                                                                                          ttCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCaaa                                                       >  1:1687153/1‑150 (MQ=255)
                                                                                           tctcCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAAc                                                      <  2:2243199/150‑1 (MQ=255)
                                                                                           tctcCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAAc                                                      >  2:260045/1‑150 (MQ=255)
                                                                                            ctcCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACg                                                     >  1:1179133/1‑150 (MQ=255)
                                                                                            ctcCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACg                                                     >  1:1592673/1‑150 (MQ=255)
                                                                                              ccccTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGAt                                                   >  2:4064938/1‑150 (MQ=255)
                                                                                                ccTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGAttt                                                 <  2:759316/150‑1 (MQ=255)
                                                                                                ccTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGAttt                                                 <  1:811952/150‑1 (MQ=255)
                                                                                                ccTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGAttt                                                 <  1:279849/150‑1 (MQ=255)
                                                                                                    ttGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGaa                                             >  2:3763523/1‑150 (MQ=255)
                                                                                                     tGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGaaa                                            <  2:1963946/150‑1 (MQ=255)
                                                                                                     tGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGaaa                                            >  1:3980356/1‑150 (MQ=255)
                                                                                                      ggTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAAc                                           >  2:2415638/1‑150 (MQ=255)
                                                                                                        tAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCa                                         >  2:2844131/1‑150 (MQ=255)
                                                                                                          aGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCCCGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGa                                       <  2:2978195/150‑1 (MQ=255)
                                                                                                           gTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGAc                                      >  1:562380/1‑150 (MQ=255)
                                                                                                             aTTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACct                                    <  2:3187321/150‑1 (MQ=255)
                                                                                                             aTTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACct                                    <  2:3188543/150‑1 (MQ=255)
                                                                                                               tttACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACctct                                  <  2:1607230/150‑1 (MQ=255)
                                                                                                                 tACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCttt                                >  2:1242673/1‑150 (MQ=255)
                                                                                                                  aCTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTg                               >  2:1830169/1‑150 (MQ=255)
                                                                                                                        gTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGc                         >  2:3105577/1‑150 (MQ=255)
                                                                                                                          tAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGccc                       >  1:1938278/1‑150 (MQ=255)
                                                                                                                           aaTGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCa                      <  1:1311277/150‑1 (MQ=255)
                                                                                                                              gtgGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCa                   >  1:891466/1‑150 (MQ=255)
                                                                                                                               tgGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCAc                  >  2:3215137/1‑150 (MQ=255)
                                                                                                                               tgGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCAc                  <  2:3612792/150‑1 (MQ=255)
                                                                                                                               tgGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCAc                  >  2:1400119/1‑150 (MQ=255)
                                                                                                                                  gATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCAt               <  1:3841110/150‑1 (MQ=255)
                                                                                                                                    tCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCg             >  1:2104016/1‑150 (MQ=255)
                                                                                                                                      tAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCggg           <  1:2707220/150‑1 (MQ=255)
                                                                                                                                         aTCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATc        >  2:726022/1‑150 (MQ=255)
                                                                                                                                         aTCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATc        <  2:1095806/150‑1 (MQ=255)
                                                                                                                                         aTCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATc        >  2:3506238/1‑150 (MQ=255)
                                                                                                                                          tCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCa       >  1:413134/1‑150 (MQ=255)
                                                                                                                                           cccAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAg      >  2:2900718/1‑150 (MQ=255)
                                                                                                                                              aGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCaa   >  2:144408/1‑150 (MQ=255)
                                                                                                                                               gCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCaaa  >  1:1394419/1‑150 (MQ=255)
                                                                                                                                               gCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCaaa  >  1:2328120/1‑150 (MQ=255)
                                                                                                                                               gCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCaaa  >  1:3030087/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AGATCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCAAA  >  CP009273/1083956‑1084248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGATCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCAAATG  >  CP009273/1083955‑1084250
                                                                                                                                                    |                                                                                                                                                   
AAGATCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTA                                                                                                                                                    <  SRR19973074.1570766/150‑1 (MQ=60)
AAGATCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTA                                                                                                                                                    >  SRR19973074.1733881/1‑150 (MQ=60)
AAGATCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTA                                                                                                                                                    >  SRR19973074.840586/1‑150 (MQ=60)
AAGATCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTA                                                                                                                                                    >  SRR19973074.2376933/1‑150 (MQ=60)
 AGATCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTAT                                                                                                                                                   <  SRR19973074.2260923/150‑1 (MQ=60)
   ATCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATAC                                                                                                                                                 <  SRR19973074.65007/150‑1 (MQ=60)
   ATCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATAC                                                                                                                                                 <  SRR19973074.64968/150‑1 (MQ=60)
        CATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCA                                                                                                                                            <  SRR19973074.2129938/150‑1 (MQ=60)
           ATAACCTGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCC                                                                                                                                         <  SRR19973074.3853686/150‑1 (MQ=60)
           ATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCC                                                                                                                                         <  SRR19973074.1263593/150‑1 (MQ=60)
           ATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCC                                                                                                                                         >  SRR19973074.3338393/1‑150 (MQ=60)
            TAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCA                                                                                                                                        >  SRR19973074.3433106/1‑150 (MQ=60)
            TAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCA                                                                                                                                        >  SRR19973074.3050980/1‑150 (MQ=60)
                ATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCACGCA                                                                                                                                    <  SRR19973074.2586944/150‑1 (MQ=60)
                   CCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAG                                                                                                                                 >  SRR19973074.4023448/1‑150 (MQ=60)
                    CAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGA                                                                                                                                >  SRR19973074.1258643/1‑150 (MQ=60)
                    CAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGA                                                                                                                                >  SRR19973074.1258648/1‑150 (MQ=60)
                     AACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGAT                                                                                                                               <  SRR19973074.890154/150‑1 (MQ=60)
                     AACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGAT                                                                                                                               >  SRR19973074.3669223/1‑150 (MQ=60)
                     AACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGAT                                                                                                                               >  SRR19973074.864797/1‑150 (MQ=60)
                     AACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTG‑TCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATG                                                                                                                              >  SRR19973074.3669213/1‑150 (MQ=60)
                       CTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGT                                                                                                                             <  SRR19973074.3351678/150‑1 (MQ=60)
                       CTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGT                                                                                                                             >  SRR19973074.321422/1‑150 (MQ=60)
                           TGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTAC                                                                                                                         >  SRR19973074.139475/1‑150 (MQ=60)
                           TGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTAC                                                                                                                         >  SRR19973074.2511804/1‑150 (MQ=60)
                           TGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTAC                                                                                                                         <  SRR19973074.2363839/150‑1 (MQ=60)
                               CTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCT                                                                                                                     <  SRR19973074.2486740/150‑1 (MQ=60)
                                  ACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAG                                                                                                                  >  SRR19973074.666742/1‑150 (MQ=60)
                                      TGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGG                                                                                                              <  SRR19973074.2614301/150‑1 (MQ=60)
                                       GTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGT                                                                                                             <  SRR19973074.202668/150‑1 (MQ=60)
                                         CATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTAC                                                                                                           <  SRR19973074.1096328/150‑1 (MQ=60)
                                             GAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAA                                                                                                       >  SRR19973074.1310184/1‑150 (MQ=60)
                                                AGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTG                                                                                                    >  SRR19973074.1768174/1‑150 (MQ=60)
                                                AGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTG                                                                                                    >  SRR19973074.991823/1‑150 (MQ=60)
                                                AGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTG                                                                                                    >  SRR19973074.2757164/1‑150 (MQ=60)
                                                AGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTG                                                                                                    >  SRR19973074.2757376/1‑150 (MQ=60)
                                                AGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTG                                                                                                    >  SRR19973074.991759/1‑150 (MQ=60)
                                                  GAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCC                                                                                                  <  SRR19973074.1125762/150‑1 (MQ=60)
                                                   AGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCA                                                                                                 >  SRR19973074.3008230/1‑150 (MQ=60)
                                                   AGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCA                                                                                                 >  SRR19973074.3355979/1‑150 (MQ=60)
                                                   AGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCA                                                                                                 >  SRR19973074.3355984/1‑150 (MQ=60)
                                                   AGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCA                                                                                                 >  SRR19973074.3355987/1‑150 (MQ=60)
                                                   AGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCA                                                                                                 >  SRR19973074.3355994/1‑150 (MQ=60)
                                                   AGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCA                                                                                                 >  SRR19973074.4163498/1‑150 (MQ=60)
                                                   AGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCA                                                                                                 <  SRR19973074.2276138/150‑1 (MQ=60)
                                                   AGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTAAACCTGAGCGGGTACGTAATTGCCA                                                                                                 >  SRR19973074.3355953/1‑150 (MQ=60)
                                                    GAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAG                                                                                                <  SRR19973074.782720/150‑1 (MQ=60)
                                                    GAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAG                                                                                                >  SRR19973074.879126/1‑150 (MQ=60)
                                                     AGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGG                                                                                               >  SRR19973074.996625/1‑150 (MQ=60)
                                                       AGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCA                                                                                             <  SRR19973074.1189852/150‑1 (MQ=60)
                                                          CGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACC                                                                                          >  SRR19973074.1577294/1‑150 (MQ=60)
                                                          CGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACC                                                                                          >  SRR19973074.1577295/1‑150 (MQ=60)
                                                          CGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACC                                                                                          >  SRR19973074.1153107/1‑150 (MQ=60)
                                                            GTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCG                                                                                        <  SRR19973074.1220381/150‑1 (MQ=60)
                                                              GATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGAC                                                                                      >  SRR19973074.2373559/1‑150 (MQ=60)
                                                               ATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACT                                                                                     <  SRR19973074.1401092/150‑1 (MQ=60)
                                                               ATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACT                                                                                     <  SRR19973074.1426981/150‑1 (MQ=60)
                                                                TATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTA                                                                                    <  SRR19973074.818551/150‑1 (MQ=60)
                                                                TATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTA                                                                                    <  SRR19973074.881481/150‑1 (MQ=60)
                                                                TATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTA                                                                                    >  SRR19973074.314662/1‑150 (MQ=60)
                                                                TATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTA                                                                                    <  SRR19973074.190809/150‑1 (MQ=60)
                                                                TATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTCTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCAAAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTA                                                                                    >  SRR19973074.752058/1‑150 (MQ=60)
                                                                 ATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAA                                                                                   <  SRR19973074.3241488/150‑1 (MQ=60)
                                                                 ATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATATTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAA                                                                                   <  SRR19973074.3241535/150‑1 (MQ=60)
                                                                   TGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAA                                                                                 >  SRR19973074.1685847/1‑150 (MQ=60)
                                                                       CAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATAT                                                                             >  SRR19973074.1733712/1‑150 (MQ=60)
                                                                        AGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATC                                                                            >  SRR19973074.349547/1‑150 (MQ=60)
                                                                         GGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCT                                                                           <  SRR19973074.1648543/150‑1 (MQ=60)
                                                                         GGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCT                                                                           <  SRR19973074.1648494/150‑1 (MQ=60)
                                                                          GATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTG                                                                          >  SRR19973074.3663452/1‑150 (MQ=60)
                                                                          GATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGAATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGAAACCCGACTAAAAATATCTG                                                                          >  SRR19973074.2668825/1‑150 (MQ=60)
                                                                              AATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTT                                                                      <  SRR19973074.1068918/150‑1 (MQ=60)
                                                                                 TTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCAT                                                                   <  SRR19973074.1826244/150‑1 (MQ=60)
                                                                                 TTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCAT                                                                   <  SRR19973074.2439823/150‑1 (MQ=60)
                                                                                      CTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTA                                                              >  SRR19973074.3857138/1‑150 (MQ=60)
                                                                                      CTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTA                                                              >  SRR19973074.1018502/1‑150 (MQ=60)
                                                                                       TTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAG                                                             >  SRR19973074.2995282/1‑150 (MQ=60)
                                                                                         TTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCA                                                           >  SRR19973074.2666995/1‑150 (MQ=60)
                                                                                          TTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAA                                                          <  SRR19973074.2247257/150‑1 (MQ=60)
                                                                                          TTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAA                                                          <  SRR19973074.826954/150‑1 (MQ=60)
                                                                                           TTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAA                                                         >  SRR19973074.1690309/1‑150 (MQ=60)
                                                                                             CTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACG                                                       >  SRR19973074.1595703/1‑150 (MQ=60)
                                                                                             CTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACG                                                       >  SRR19973074.1181378/1‑150 (MQ=60)
                                                                                             CTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACG                                                       >  SRR19973074.1053438/1‑150 (MQ=60)
                                                                                                 CCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTT                                                   <  SRR19973074.280383/150‑1 (MQ=60)
                                                                                                 CCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTT                                                   <  SRR19973074.280378/150‑1 (MQ=60)
                                                                                                 CCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTT                                                   <  SRR19973074.813515/150‑1 (MQ=60)
                                                                                                     TTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAA                                               <  SRR19973074.2283609/150‑1 (MQ=60)
                                                                                                      TGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAA                                              >  SRR19973074.3987695/1‑150 (MQ=60)
                                                                                                         TAAGTATTTTACTCGCGTTTATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCA                                           <  SRR19973074.786043/150‑1 (MQ=60)
                                                                                                            GTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGAC                                        >  SRR19973074.563487/1‑150 (MQ=60)
                                                                                                             TATTTTACTCGCGTTAATGTGGGATCTAACTCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAAACCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACC                                       <  SRR19973074.2617174/150‑1 (MQ=60)
                                                                                                                 TTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTT                                   >  SRR19973074.1554093/1‑150 (MQ=60)
                                                                                                                  TACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTT                                  >  SRR19973074.858868/1‑150 (MQ=60)
                                                                                                                   ACTCGCGTTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTG                                 <  SRR19973074.1310592/150‑1 (MQ=60)
                                                                                                                          TTAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCC                          >  SRR19973074.3805047/1‑150 (MQ=60)
                                                                                                                           TAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCC                         >  SRR19973074.1941983/1‑150 (MQ=60)
                                                                                                                           TAATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCC                         >  SRR19973074.2796420/1‑150 (MQ=60)
                                                                                                                            AATGTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCA                        <  SRR19973074.1313745/150‑1 (MQ=60)
                                                                                                                               GTGGGATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCA                     >  SRR19973074.893171/1‑150 (MQ=60)
                                                                                                                                TGGGATCTAAATCCCAGCTTTATACCTGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGACACCAGACCTCTTTGACCAGCCCATCAC                    >  SRR19973074.3429470/1‑150 (MQ=60)
                                                                                                                                   GATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCAT                 <  SRR19973074.2092410/150‑1 (MQ=60)
                                                                                                                                   GATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCAT                 <  SRR19973074.3848108/150‑1 (MQ=60)
                                                                                                                                    ATCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATC                >  SRR19973074.1699350/1‑150 (MQ=60)
                                                                                                                                     TCTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCG               >  SRR19973074.2107957/1‑150 (MQ=60)
                                                                                                                                      CTAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGG              <  SRR19973074.3849157/150‑1 (MQ=60)
                                                                                                                                       TAAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGG             <  SRR19973074.601098/150‑1 (MQ=60)
                                                                                                                                       TAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGG             <  SRR19973074.2712237/150‑1 (MQ=60)
                                                                                                                                        AAATCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGA            >  SRR19973074.1414520/1‑150 (MQ=60)
                                                                                                                                           TCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCA         >  SRR19973074.413924/1‑150 (MQ=60)
                                                                                                                                           TCCCAGCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCA         <  SRR19973074.1971125/150‑1 (MQ=60)
                                                                                                                                                GCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCAAA    >  SRR19973074.2332866/1‑150 (MQ=60)
                                                                                                                                                GCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCAAA    >  SRR19973074.2332464/1‑150 (MQ=60)
                                                                                                                                                GCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCAAA    >  SRR19973074.1397319/1‑150 (MQ=60)
                                                                                                                                                GCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCAAA    >  SRR19973074.3035691/1‑150 (MQ=60)
                                                                                                                                                GCTTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCAAA    >  SRR19973074.1396980/1‑150 (MQ=60)
                                                                                                                                                  TTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCAAATG  <  SRR19973074.1237528/150‑1 (MQ=60)
                                                                                                                                                  TTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCAAATG  <  SRR19973074.1122490/150‑1 (MQ=60)
                                                                                                                                                  TTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCAAATG  <  SRR19973074.1237834/150‑1 (MQ=60)
                                                                                                                                                  TTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCAAATG  <  SRR19973074.133936/150‑1 (MQ=60)
                                                                                                                                                  TTTATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCAAATG  <  SRR19973074.133981/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
AAGATCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATATCTGCTTTCATTGGTAGCAAACGATTTGGAAACCAGACCTCTTTGACCAGCCCATCACCATCGGGATCAGCAAATG  >  CP009273/1083955‑1084250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑