Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,207,417 C→A 28.7% G15C (GGT→TGT)  iraM ← RpoS stabilzer during Mg starvation, anti‑RssB factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,207,4170CA28.7% 739.1 / 253.6 372G15C (GGT→TGT) iraMRpoS stabilzer during Mg starvation, anti‑RssB factor
Reads supporting (aligned to +/- strand):  ref base C (137/128);  new base A (52/54);  total (189/183)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 384 total aligned reads displayed.

GATGTGATAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCCTCAAAAAAATAAATTAATTAAA  >  CP009273/1207268‑1207566
                                                                                                                                                     |                                                                                                                                                      
gATGTGATAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATAcc                                                                                                                                                        >  2:580410/1‑150 (MQ=255)
   gtgATAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACcaca                                                                                                                                                     <  1:3473086/150‑1 (MQ=255)
   gtgATAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACcaca                                                                                                                                                     >  1:887952/1‑150 (MQ=255)
    tgATAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACAt                                                                                                                                                    <  2:4049918/150‑1 (MQ=255)
    tgATAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACAt                                                                                                                                                    >  2:4170823/1‑150 (MQ=255)
     gATAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATg                                                                                                                                                   >  2:1825707/1‑150 (MQ=255)
      aTAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGt                                                                                                                                                  >  2:2898528/1‑150 (MQ=255)
      aTAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGt                                                                                                                                                  <  2:4824173/150‑1 (MQ=255)
      aTAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACAACATGt                                                                                                                                                  >  2:328848/1‑150 (MQ=255)
        aaaTAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAg                                                                                                                                                <  2:3186286/150‑1 (MQ=255)
        aaaTAGTAATAGGATATTC‑TTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACAACATGTAgg                                                                                                                                               >  1:4305483/1‑150 (MQ=255)
         aaTAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAgg                                                                                                                                               >  1:798062/1‑150 (MQ=255)
         aaTAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACAACATGTAgg                                                                                                                                               >  2:3913609/1‑150 (MQ=255)
            aGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTg                                                                                                                                            <  1:140442/150‑1 (MQ=255)
            aGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTg                                                                                                                                            >  1:3210086/1‑150 (MQ=255)
             gTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGa                                                                                                                                           >  2:2123262/1‑150 (MQ=255)
               aaTAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAAt                                                                                                                                         >  2:1375774/1‑150 (MQ=255)
               aaTAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAAt                                                                                                                                         >  1:2317017/1‑150 (MQ=255)
               aaTAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAAt                                                                                                                                         >  2:4104508/1‑150 (MQ=255)
                  aGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTAc                                                                                                                                      >  1:4279804/1‑150 (MQ=255)
                   ggATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTAcc                                                                                                                                     >  2:3469851/1‑150 (MQ=255)
                       atTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCgtgt                                                                                                                                 >  2:4007026/1‑150 (MQ=255)
                       atTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACAACATGTAGGTTGAATTACCgtgt                                                                                                                                 >  1:3291347/1‑150 (MQ=255)
                        ttCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTc                                                                                                                                >  1:458536/1‑150 (MQ=255)
                         tCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCa                                                                                                                               <  1:1376754/150‑1 (MQ=255)
                           tttATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAAt                                                                                                                             <  2:1146158/150‑1 (MQ=255)
                           tttATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACAACATGTAGGTTGAATTACCGTGTCAAt                                                                                                                             <  2:1893965/150‑1 (MQ=255)
                             tATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTa                                                                                                                           >  2:2193476/1‑150 (MQ=255)
                                      ggATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACt                                                                                                                  <  2:2404420/150‑1 (MQ=255)
                                      ggATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACt                                                                                                                  <  1:2203222/150‑1 (MQ=255)
                                       gATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACtt                                                                                                                 <  2:2280500/150‑1 (MQ=255)
                                       gATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACtt                                                                                                                 >  1:119202/1‑150 (MQ=255)
                                       gATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACAACATGTAGGTTGAATTACCGTGTCAATTACTATCCACtt                                                                                                                 >  1:3065256/1‑150 (MQ=255)
                                        ataataCCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTc                                                                                                                <  2:2973481/150‑1 (MQ=255)
                                         taataCCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACAACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCa                                                                                                               >  2:923432/1‑150 (MQ=255)
                                             aCCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTg                                                                                                           >  2:1710579/1‑150 (MQ=255)
                                                  aCTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATg                                                                                                      <  1:3552949/150‑1 (MQ=255)
                                                   cTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGt                                                                                                     <  2:2505701/150‑1 (MQ=255)
                                                    ttAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTc                                                                                                    <  2:5009402/150‑1 (MQ=255)
                                                     tAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACAACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCt                                                                                                   >  2:3104707/1‑150 (MQ=255)
                                                      aaCCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACAACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCtt                                                                                                  <  2:3824344/150‑1 (MQ=255)
                                                       aCCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTa                                                                                                 <  2:3426470/150‑1 (MQ=255)
                                                       aCCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACAACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTa                                                                                                 >  2:4464777/1‑150 (MQ=255)
                                                          ggTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATcc                                                                                              <  1:3677175/150‑1 (MQ=255)
                                                          ggTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATcc                                                                                              <  2:1591839/150‑1 (MQ=255)
                                                            tgtAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCa                                                                                            >  2:2064355/1‑150 (MQ=255)
                                                            tgtAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACAACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCa                                                                                            <  1:1008864/150‑1 (MQ=255)
                                                                aaTATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGt                                                                                        >  2:4438458/1‑150 (MQ=255)
                                                                 atatACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTa                                                                                       >  1:4657610/1‑150 (MQ=255)
                                                                 atatACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTa                                                                                       >  1:574202/1‑150 (MQ=255)
                                                                   ataCTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACAACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTAtt                                                                                     <  2:356242/150‑1 (MQ=255)
                                                                     aCTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTa                                                                                   <  2:1201962/150‑1 (MQ=255)
                                                                      cTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTaa                                                                                  <  1:2632334/150‑1 (MQ=255)
                                                                         ccAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAatat                                                                               <  1:1040830/150‑1 (MQ=255)
                                                                         ccAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAatat                                                                               <  2:785901/150‑1 (MQ=255)
                                                                           aggaggGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACAACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATgg                                                                             <  2:394389/150‑1 (MQ=255)
                                                                               gggagAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCa                                                                         <  1:4020842/150‑1 (MQ=255)
                                                                                   gaAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACAACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAg                                                                     <  1:4853980/150‑1 (MQ=255)
                                                                                    aaaTATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAgg                                                                    <  1:4241707/150‑1 (MQ=255)
                                                                                     aaTATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGa                                                                   >  1:2024137/1‑150 (MQ=255)
                                                                                      atatAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGAt                                                                  <  2:3696830/150‑1 (MQ=255)
                                                                                      atatAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGAt                                                                  >  2:2379584/1‑150 (MQ=255)
                                                                                       tataGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACAACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATg                                                                 <  2:2704639/150‑1 (MQ=255)
                                                                                          agtagATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGttt                                                              <  1:155809/150‑1 (MQ=255)
                                                                                             agATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTAtt                                                           >  1:4082554/1‑150 (MQ=255)
                                                                                             agATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCAACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTAtt                                                           >  1:1764647/1‑150 (MQ=255)
                                                                                             agATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACAACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCAATAGGATGTTTAtt                                                           >  2:1302505/1‑150 (MQ=255)
                                                                                               aTTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTc                                                         <  1:1512828/150‑1 (MQ=255)
                                                                                                   aTACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACAACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGa                                                     >  1:2481208/1‑150 (MQ=255)
                                                                                                      ccAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGTTGTTTATTTCTTGAttt                                                  <  2:4291066/150‑1 (MQ=255)
                                                                                                      ccAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACAACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGAttt                                                  >  1:5023538/1‑150 (MQ=255)
                                                                                                        aGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACAACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTg                                                >  1:131290/1‑150 (MQ=255)
                                                                                                        aGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACAACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTg                                                >  2:4590512/1‑150 (MQ=255)
                                                                                                             atCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCatat                                           <  1:3931194/150‑1 (MQ=255)
                                                                                                               cATTTTCATATTACCCCATATGGCTGAAAAAGATATACAACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGa                                         >  1:4785617/1‑150 (MQ=255)
                                                                                                                aTTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAg                                        <  1:2119203/150‑1 (MQ=255)
                                                                                                                   ttCATATTACCCCATATGGCTGAAAAAGATATACAACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGtat                                     <  2:274510/150‑1 (MQ=255)
                                                                                                                   ttCATATTACCCCATATGGCTGAAAAAGATATACAACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGtat                                     <  2:3363/150‑1 (MQ=255)
                                                                                                                      atatTACCCCATATGGCTGAAAAAGATATACAACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATAtt                                  >  1:4399430/1‑150 (MQ=255)
                                                                                                                      atatTACCCCATATGGCTGAAAAAGATATACAACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATAtt                                  >  1:2350074/1‑150 (MQ=255)
                                                                                                                        atTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑c                               <  1:995655/150‑1 (MQ=255)
                                                                                                                         ttACCCCATATGGCTGAAAAAGATATACAACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑cc                              >  2:870524/1‑150 (MQ=255)
                                                                                                                             cccATATGGCTGAAAAAGATATACAACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTCTATTA‑cccccc                          >  1:4928145/1‑150 (MQ=255)
                                                                                                                               cATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑cccccccc                        >  1:2804811/1‑150 (MQ=255)
                                                                                                                                 tatGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCCTc                      >  1:210481/1‑150 (MQ=255)
                                                                                                                                  atGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCCTCa                     <  2:1959516/150‑1 (MQ=255)
                                                                                                                                  atGGCTGAAAAAGATATACAACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTCTCTTAGCCCCCCCCTc                      >  1:31203/1‑150 (MQ=255)
                                                                                                                                    ggCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCCTCaaa                   <  2:325089/150‑1 (MQ=255)
                                                                                                                                    ggCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCCTCaaa                   <  1:926825/150‑1 (MQ=255)
                                                                                                                                     gCTGAAAAAGATATACCACATGTAGTTTGAATTACCGTGTAAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTTCATATGAGTATATTA‑CCCCCCCCTCaaaa                  <  2:3843593/150‑1 (MQ=255)
                                                                                                                                        gAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCCTCaaaaaaa               >  2:3056515/1‑150 (MQ=255)
                                                                                                                                         aaaaaGATATACAACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCCTCAAAAaaat              <  1:5151550/150‑1 (MQ=255)
                                                                                                                                           aaaGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCCTCAAAAaaataa            >  2:5161110/1‑150 (MQ=255)
                                                                                                                                            aaGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCCTCAAAAaaataaa           >  2:3151322/1‑150 (MQ=255)
                                                                                                                                            aaGATATACAACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCCTCAAAAaaataaa           >  1:3735613/1‑150 (MQ=255)
                                                                                                                                             aGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCCTCAAAAaaataaat          <  1:2898528/150‑1 (MQ=255)
                                                                                                                                              gATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCCTCAAAAAAATAaatt         <  2:3656143/150‑1 (MQ=255)
                                                                                                                                              gATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCCTCAAAAAAATAaatt         <  1:513177/150‑1 (MQ=255)
                                                                                                                                                tataCCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCCTCAAAAAAATAaattaa       <  1:4527457/150‑1 (MQ=255)
                                                                                                                                                 ataCCACATGTAGGTTGAATTAGCGTGTCAATTACTATCCACGTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTAATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCCTCAAAAAAATAaattaat      <  2:1091836/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
GATGTGATAAATAGTAATAGGATATTCTTTATCCTTAAGGATAATACCAGACTTAACCGGTGTAAATATACTGCCAGGAGGGAGAAATATAGTAGATTGATACCAGATGATCATTTTCATATTACCCCATATGGCTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCCTCAAAAAAATAAATTAATTAAA  >  CP009273/1207268‑1207566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A