Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,211,169 C→A 93.1% intergenic (‑238/‑76) bluF ← / → ycgZ anti‑repressor for YcgE, blue light‑responsive; FAD‑binding; has c‑di‑GMP phosphodiesterase‑like EAL domain, but does not degrade c‑di‑GMP/RcsB connector protein for regulation of biofilm and acid‑resistance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,211,1690CA93.1% 802.8 / 48.7 262intergenic (‑238/‑76)bluF/ycgZanti‑repressor for YcgE, blue light‑responsive; FAD‑binding; has c‑di‑GMP phosphodiesterase‑like EAL domain, but does not degrade c‑di‑GMP/RcsB connector protein for regulation of biofilm and acid‑resistance
Reads supporting (aligned to +/- strand):  ref base C (7/11);  new base A (113/131);  total (120/142)
Fisher's exact test for biased strand distribution p-value = 6.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 262 total aligned reads displayed.

TCTTTACATTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTCTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCAAAAGCTAACTTGCA  >  CP009273/1211022‑1211315
                                                                                                                                                   |                                                                                                                                                  
tCTTTACATTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATg                                                                                                                                                  >  1:671738/1‑150 (MQ=255)
tCTTTACATTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATg                                                                                                                                                  <  2:1982759/150‑1 (MQ=255)
 cTTTACATTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGt                                                                                                                                                 <  2:3022293/150‑1 (MQ=255)
 cTTTACATTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGt                                                                                                                                                 >  1:3801288/1‑150 (MQ=255)
  tttACATTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTa                                                                                                                                                >  1:950479/1‑150 (MQ=255)
  tttACATTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTa                                                                                                                                                <  1:2102374/150‑1 (MQ=255)
  tttACATTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTa                                                                                                                                                <  1:1184298/150‑1 (MQ=255)
   ttACATTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTac                                                                                                                                               <  1:518211/150‑1 (MQ=255)
     aCATTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTacac                                                                                                                                             <  2:1522416/150‑1 (MQ=255)
      cATTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTAcaca                                                                                                                                            >  1:2571567/1‑150 (MQ=255)
       aTTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACat                                                                                                                                           >  1:2486187/1‑150 (MQ=255)
           aTAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATAttt                                                                                                                                       >  1:4096638/1‑150 (MQ=255)
             aaaaaaTGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTCTGTACACATATTTCg                                                                                                                                     >  2:2545587/1‑150 (MQ=255)
              aaaaaTGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGt                                                                                                                                    >  1:2643217/1‑150 (MQ=255)
              aaaaaTGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGt                                                                                                                                    <  1:251005/150‑1 (MQ=255)
              aaaaaTGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGt                                                                                                                                    >  1:680804/1‑150 (MQ=255)
                aaaTGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTAc                                                                                                                                  >  1:3724702/1‑150 (MQ=255)
                     gAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGtt                                                                                                                             <  2:2357041/150‑1 (MQ=255)
                      aaaaaGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGttt                                                                                                                            >  2:404573/1‑150 (MQ=255)
                       aaaaGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTg                                                                                                                           <  1:1283381/150‑1 (MQ=255)
                        aaaGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGc                                                                                                                          <  1:739066/150‑1 (MQ=255)
                        aaaGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGc                                                                                                                          <  1:1672344/150‑1 (MQ=255)
                        aaaGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGc                                                                                                                          >  1:2878453/1‑150 (MQ=255)
                          aGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTa                                                                                                                        <  1:2922155/150‑1 (MQ=255)
                               aaCACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGtt                                                                                                                   >  2:1758013/1‑150 (MQ=255)
                                acacTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTa                                                                                                                  >  1:81163/1‑150 (MQ=255)
                                acacTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTa                                                                                                                  <  1:434009/150‑1 (MQ=255)
                                  acTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTCTGTACACATATTTCGTACAAGTTTGCTATTGTTACt                                                                                                                >  1:4138453/1‑150 (MQ=255)
                                   cTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACtt                                                                                                               >  1:239652/1‑150 (MQ=255)
                                    tAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAACTGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTc                                                                                                              <  2:2440315/150‑1 (MQ=255)
                                     aGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCa                                                                                                             <  1:2788566/150‑1 (MQ=255)
                                        tGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACtt                                                                                                          >  2:1290834/1‑150 (MQ=255)
                                            gAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACa                                                                                                      <  1:3009422/150‑1 (MQ=255)
                                            gAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACa                                                                                                      >  1:624887/1‑150 (MQ=255)
                                             aaaaaTCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACAt                                                                                                     <  1:1044405/150‑1 (MQ=255)
                                                 aTCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTCTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGAt                                                                                                 <  2:2115104/150‑1 (MQ=255)
                                                 aTCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTCTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGAt                                                                                                 <  2:3246840/150‑1 (MQ=255)
                                                 aTCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTCTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGAt                                                                                                 <  2:1154878/150‑1 (MQ=255)
                                                 aTCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGAt                                                                                                 <  2:1607680/150‑1 (MQ=255)
                                                 aTCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGAt                                                                                                 <  2:3208403/150‑1 (MQ=255)
                                                  tCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTCTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGAtt                                                                                                <  2:86383/150‑1 (MQ=255)
                                                         taGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACAttt                                                                                         <  1:1215196/150‑1 (MQ=255)
                                                         taGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACAttt                                                                                         >  1:3337665/1‑150 (MQ=255)
                                                          aGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACAtttt                                                                                        <  2:1893095/150‑1 (MQ=255)
                                                           gATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACAttttt                                                                                       <  2:335475/150‑1 (MQ=255)
                                                           gATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACAttttt                                                                                       <  1:1343628/150‑1 (MQ=255)
                                                            aTTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTa                                                                                      >  1:1446420/1‑150 (MQ=255)
                                                               gTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACa                                                                                   <  2:3690408/150‑1 (MQ=255)
                                                                tCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACag                                                                                  >  1:936607/1‑150 (MQ=255)
                                                                tCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACag                                                                                  <  2:2211388/150‑1 (MQ=255)
                                                                    ttAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGc                                                                              <  1:349871/150‑1 (MQ=255)
                                                                          gatgCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAgca                                                                        <  2:3050909/150‑1 (MQ=255)
                                                                          gatgCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAgca                                                                        >  2:3604960/1‑150 (MQ=255)
                                                                            tgCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAgcatg                                                                      >  1:200414/1‑150 (MQ=255)
                                                                             gCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAgcatgc                                                                     >  2:3367129/1‑150 (MQ=255)
                                                                              cAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAgcatgca                                                                    <  2:622956/150‑1 (MQ=255)
                                                                              cAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAgcatgca                                                                    <  2:1883208/150‑1 (MQ=255)
                                                                              cAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAATTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAgcatgca                                                                    >  2:4053220/1‑150 (MQ=255)
                                                                               aaTATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAgcatgcat                                                                   >  1:2804133/1‑150 (MQ=255)
                                                                                  atGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCaa                                                                >  2:2663235/1‑150 (MQ=255)
                                                                                    gTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCaaaa                                                              >  1:1902671/1‑150 (MQ=255)
                                                                                     ttttATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAAt                                                             >  2:3068064/1‑150 (MQ=255)
                                                                                     ttttATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAAt                                                             <  2:505706/150‑1 (MQ=255)
                                                                                      tttATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAAtt                                                            >  1:1728477/1‑150 (MQ=255)
                                                                                      tttATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAAtt                                                            >  1:3448942/1‑150 (MQ=255)
                                                                                      tttATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAAtt                                                            >  1:3164312/1‑150 (MQ=255)
                                                                                       ttATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTc                                                           >  1:3704039/1‑150 (MQ=255)
                                                                                       ttATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTc                                                           >  2:341301/1‑150 (MQ=255)
                                                                                       ttATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAATTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTc                                                           >  1:2280077/1‑150 (MQ=255)
                                                                                        tATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCa                                                          <  2:1712518/150‑1 (MQ=255)
                                                                                        tATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCa                                                          <  2:2783605/150‑1 (MQ=255)
                                                                                         aTCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAg                                                         >  2:3916975/1‑150 (MQ=255)
                                                                                           catacaacaTTGTTTTATATGCATGAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGTTTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTg                                                       <  1:416851/144‑1 (MQ=255)
                                                                                              aaCACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACt                                                    <  2:111981/150‑1 (MQ=255)
                                                                                              aaCACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACt                                                    <  2:2902897/150‑1 (MQ=255)
                                                                                              aaCACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACt                                                    <  1:613289/150‑1 (MQ=255)
                                                                                               acacATTGTTTTAAATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACtt                                                   >  2:3595610/1‑150 (MQ=255)
                                                                                                cacaTTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACttt                                                  <  2:2735308/150‑1 (MQ=255)
                                                                                                  caTTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAg                                                <  1:1520432/150‑1 (MQ=255)
                                                                                                   aTTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGa                                               >  2:3263969/1‑150 (MQ=255)
                                                                                                     tGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGAtt                                             <  1:976049/150‑1 (MQ=255)
                                                                                                          tataTGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCg                                        <  1:4029066/150‑1 (MQ=255)
                                                                                                            tatGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCggg                                      >  2:947264/1‑150 (MQ=255)
                                                                                                            tatGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCggg                                      <  1:857380/150‑1 (MQ=255)
                                                                                                             atGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGa                                     >  1:2332484/1‑150 (MQ=255)
                                                                                                                 aTTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCaa                                 <  1:2830266/150‑1 (MQ=255)
                                                                                                                  ttAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAAt                                <  1:1055021/150‑1 (MQ=255)
                                                                                                                   tAGCACTAATTGCAAAAAATTAATTTATCATTCTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATc                               <  1:2360651/150‑1 (MQ=255)
                                                                                                                        cTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCg                          >  2:1976726/1‑150 (MQ=255)
                                                                                                                         tAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGt                         >  2:4141265/1‑150 (MQ=255)
                                                                                                                         tAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGt                         >  2:808226/1‑150 (MQ=255)
                                                                                                                          aaTTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGtt                        <  2:3877827/150‑1 (MQ=255)
                                                                                                                           aTTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTa                       >  2:1068112/1‑150 (MQ=255)
                                                                                                                               cAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACttt                   <  1:2715528/150‑1 (MQ=255)
                                                                                                                               cAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACttt                   >  1:2030578/1‑150 (MQ=255)
                                                                                                                                aaaaaaTTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTg                  >  1:1640217/1‑150 (MQ=255)
                                                                                                                                  aaaaTTAATTTATCTTTATGTACACATATTTAGTACAAGTTTGCTATTGTTACTTCTCTTAAGATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCa                <  1:3487594/150‑1 (MQ=255)
                                                                                                                                            tATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCAAAAGCTAACt      <  1:2168762/150‑1 (MQ=255)
                                                                                                                                             aacATTCTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCAAAAGCTAACtt     <  2:2216900/148‑1 (MQ=255)
                                                                                                                                                aTTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCAAAAGCTAACTTGCa  <  2:3849999/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCTTTACATTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTCTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCAAAAGCTAACTTGCA  >  CP009273/1211022‑1211315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTCTTTACATTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTCTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCAAAAGCTAACTTGCATA  >  CP009273/1211020‑1211317
                                                                                                                                                     |                                                                                                                                                    
TTTCTTTACATTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTA                                                                                                                                                      <  SRR19973074.3430783/150‑1 (MQ=60)
TTTCTTTACATTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTA                                                                                                                                                      >  SRR19973074.3884927/1‑150 (MQ=60)
TTTCTTTACATTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTA                                                                                                                                                      <  SRR19973074.1614214/150‑1 (MQ=60)
TTTCTTTACATTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTA                                                                                                                                                      >  SRR19973074.3884850/1‑150 (MQ=60)
TTTCTTTACATTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTA                                                                                                                                                      <  SRR19973074.3430856/150‑1 (MQ=60)
  TCTTTACATTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATG                                                                                                                                                    >  SRR19973074.672974/1‑150 (MQ=60)
  TCTTTACATTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATG                                                                                                                                                    >  SRR19973074.856173/1‑150 (MQ=60)
   CTTTACATTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGT                                                                                                                                                   >  SRR19973074.3808246/1‑150 (MQ=60)
    TTTACATTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTA                                                                                                                                                  >  SRR19973074.952285/1‑150 (MQ=60)
    TTTACATTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTA                                                                                                                                                  <  SRR19973074.1186557/150‑1 (MQ=60)
    TTTACATTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTA                                                                                                                                                  <  SRR19973074.495095/150‑1 (MQ=60)
    TTTACATTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTA                                                                                                                                                  <  SRR19973074.2106314/150‑1 (MQ=60)
     TTACATTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTAC                                                                                                                                                 <  SRR19973074.519247/150‑1 (MQ=60)
       ACATTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACAC                                                                                                                                               <  SRR19973074.3170767/150‑1 (MQ=60)
        CATTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACA                                                                                                                                              >  SRR19973074.2576370/1‑150 (MQ=60)
        CATTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACA                                                                                                                                              <  SRR19973074.2740263/150‑1 (MQ=60)
         ATTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACAT                                                                                                                                             >  SRR19973074.2490804/1‑150 (MQ=60)
         ATTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACAT                                                                                                                                             >  SRR19973074.2490878/1‑150 (MQ=60)
             ATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTT                                                                                                                                         >  SRR19973074.4104205/1‑150 (MQ=60)
               AAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCG                                                                                                                                       <  SRR19973074.640349/150‑1 (MQ=60)
               AAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCG                                                                                                                                       >  SRR19973074.2993922/1‑150 (MQ=60)
                AAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGT                                                                                                                                      >  SRR19973074.682049/1‑150 (MQ=60)
                AAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGT                                                                                                                                      >  SRR19973074.682057/1‑150 (MQ=60)
                AAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGT                                                                                                                                      >  SRR19973074.2648148/1‑150 (MQ=60)
                AAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGT                                                                                                                                      <  SRR19973074.251394/150‑1 (MQ=60)
                AAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGT                                                                                                                                      <  SRR19973074.251501/150‑1 (MQ=60)
                 AAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTA                                                                                                                                     <  SRR19973074.683318/150‑1 (MQ=60)
                  AAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTAC                                                                                                                                    <  SRR19973074.1080373/150‑1 (MQ=60)
                  AAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTAC                                                                                                                                    >  SRR19973074.3731674/1‑150 (MQ=60)
                  AAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTAC                                                                                                                                    >  SRR19973074.3731569/1‑150 (MQ=60)
                  AAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTAC                                                                                                                                    >  SRR19973074.3731555/1‑150 (MQ=60)
                  AAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATAGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTATACATATTTCGTAC                                                                                                                                    >  SRR19973074.146252/1‑150 (MQ=60)
                   AATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTAGA                                                                                                                                   >  SRR19973074.2489274/1‑150 (MQ=60)
                        AAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTT                                                                                                                              <  SRR19973074.314853/150‑1 (MQ=60)
                        AAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTT                                                                                                                              >  SRR19973074.2054986/1‑150 (MQ=60)
                        AAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTT                                                                                                                              <  SRR19973074.2990489/150‑1 (MQ=60)
                        AAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGACAGTTAAATGATGCAATATGTTTAATCATAACACATAGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACAAATTTCGAAAAAGTTT                                                                                                                              >  SRR19973074.426370/1‑150 (MQ=60)
                         AAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTG                                                                                                                             <  SRR19973074.1285823/150‑1 (MQ=60)
                          AAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGC                                                                                                                            <  SRR19973074.1675488/150‑1 (MQ=60)
                          AAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGC                                                                                                                            >  SRR19973074.2883794/1‑150 (MQ=60)
                          AAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGC                                                                                                                            <  SRR19973074.740468/150‑1 (MQ=60)
                          AAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGC                                                                                                                            <  SRR19973074.740453/150‑1 (MQ=60)
                           AAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCT                                                                                                                           <  SRR19973074.1554365/150‑1 (MQ=60)
                            AGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTA                                                                                                                          <  SRR19973074.2927587/150‑1 (MQ=60)
                            AGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTA                                                                                                                          <  SRR19973074.2927413/150‑1 (MQ=60)
                            AGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTA                                                                                                                          <  SRR19973074.2242324/150‑1 (MQ=60)
                             GTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTAT                                                                                                                         >  SRR19973074.3655953/1‑150 (MQ=60)
                                  ACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTA                                                                                                                    <  SRR19973074.434845/150‑1 (MQ=60)
                                  ACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTA                                                                                                                    >  SRR19973074.81364/1‑150 (MQ=60)
                                  ACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTA                                                                                                                    >  SRR19973074.2165054/1‑150 (MQ=60)
                                    ACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTCTGTACACATATTTCGTACAAGTTTGCTATTGTTACT                                                                                                                  >  SRR19973074.4146232/1‑150 (MQ=60)
                                    ACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTCTGTACACATATTTCGTACAAGTTTGCTATTGTTACT                                                                                                                  >  SRR19973074.4146079/1‑150 (MQ=60)
                                     CTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTT                                                                                                                 >  SRR19973074.240136/1‑150 (MQ=60)
                                     CTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTT                                                                                                                 >  SRR19973074.364925/1‑150 (MQ=60)
                                       AGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCA                                                                                                               >  SRR19973074.1491013/1‑150 (MQ=60)
                                       AGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCA                                                                                                               <  SRR19973074.2793695/150‑1 (MQ=60)
                                       AGTTGGAGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCA                                                                                                               <  SRR19973074.702058/150‑1 (MQ=60)
                                             CGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAAC                                                                                                         <  SRR19973074.437840/150‑1 (MQ=60)
                                              GAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACA                                                                                                        >  SRR19973074.626061/1‑150 (MQ=60)
                                              GAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACA                                                                                                        <  SRR19973074.3015000/150‑1 (MQ=60)
                                              GAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACA                                                                                                        <  SRR19973074.1309392/150‑1 (MQ=60)
                                               AAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACAT                                                                                                       <  SRR19973074.1046325/150‑1 (MQ=60)
                                               AAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACAT                                                                                                       >  SRR19973074.963722/1‑150 (MQ=60)
                                                AAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATT                                                                                                      >  SRR19973074.3058889/1‑150 (MQ=60)
                                                  AATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGA                                                                                                    >  SRR19973074.2613726/1‑150 (MQ=60)
                                                     CTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTA                                                                                                 >  SRR19973074.3769155/1‑150 (MQ=60)
                                                          ATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATT                                                                                            >  SRR19973074.1203991/1‑150 (MQ=60)
                                                           TAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTT                                                                                           >  SRR19973074.3343859/1‑150 (MQ=60)
                                                           TAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTT                                                                                           <  SRR19973074.1217524/150‑1 (MQ=60)
                                                            AGATTTTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTT                                                                                          <  SRR19973074.555333/150‑1 (MQ=60)
                                                             GATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTT                                                                                         <  SRR19973074.2550486/150‑1 (MQ=60)
                                                             GATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTT                                                                                         <  SRR19973074.2849014/150‑1 (MQ=60)
                                                             GATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTT                                                                                         <  SRR19973074.2849047/150‑1 (MQ=60)
                                                             GATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTT                                                                                         <  SRR19973074.1346136/150‑1 (MQ=60)
                                                              ATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTA                                                                                        >  SRR19973074.1449123/1‑150 (MQ=60)
                                                               TTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTCTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAA                                                                                       >  SRR19973074.3480615/1‑150 (MQ=60)
                                                                 GTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACA                                                                                     <  SRR19973074.4155382/150‑1 (MQ=60)
                                                                 GTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACA                                                                                     >  SRR19973074.4070213/1‑150 (MQ=60)
                                                                  TCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAG                                                                                    >  SRR19973074.938556/1‑150 (MQ=60)
                                                                  TCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAG                                                                                    >  SRR19973074.938390/1‑150 (MQ=60)
                                                                      TTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGC                                                                                <  SRR19973074.350504/150‑1 (MQ=60)
                                                                      TTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGC                                                                                <  SRR19973074.1693931/150‑1 (MQ=60)
                                                                              TGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATG                                                                        >  SRR19973074.200835/1‑150 (MQ=60)
                                                                              TGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATG                                                                        >  SRR19973074.200859/1‑150 (MQ=60)
                                                                                 AATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCAT                                                                     >  SRR19973074.2809309/1‑150 (MQ=60)
                                                                                     TGTTTTATCATAACACATTGTTTTATATTCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAA                                                                 <  SRR19973074.2445358/150‑1 (MQ=60)
                                                                                      GTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAA                                                                >  SRR19973074.1906317/1‑150 (MQ=60)
                                                                                      GTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAA                                                                >  SRR19973074.1906421/1‑150 (MQ=60)
                                                                                       TTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAAT                                                               >  SRR19973074.1667555/1‑150 (MQ=60)
                                                                                        TTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATT                                                              >  SRR19973074.3170134/1‑150 (MQ=60)
                                                                                        TTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATT                                                              >  SRR19973074.1731680/1‑150 (MQ=60)
                                                                                        TTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATT                                                              <  SRR19973074.965447/150‑1 (MQ=60)
                                                                                        TTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATT                                                              >  SRR19973074.3455282/1‑150 (MQ=60)
                                                                                        TTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATT                                                              >  SRR19973074.3455285/1‑150 (MQ=60)
                                                                                        TTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATGTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATT                                                              >  SRR19973074.3455256/1‑150 (MQ=60)
                                                                                         TTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTC                                                             >  SRR19973074.4106752/1‑150 (MQ=60)
                                                                                         TTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTC                                                             >  SRR19973074.1402880/1‑150 (MQ=60)
                                                                                         TTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTC                                                             >  SRR19973074.3710885/1‑150 (MQ=60)
                                                                                         TTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAATTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTC                                                             >  SRR19973074.2284356/1‑150 (MQ=60)
                                                                                          TATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCA                                                            >  SRR19973074.413775/1‑150 (MQ=60)
                                                                                            TCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGT                                                          <  SRR19973074.77143/150‑1 (MQ=60)
                                                                                             CATACAACATTGTTTTATATGCATGAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGTTTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTG                                                         <  SRR19973074.417654/150‑1 (MQ=60)
                                                                                              ATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGA                                                        >  SRR19973074.652886/1‑150 (MQ=60)
                                                                                                AACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACT                                                      <  SRR19973074.614452/150‑1 (MQ=60)
                                                                                                AACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACT                                                      <  SRR19973074.614466/150‑1 (MQ=60)
                                                                                                 ACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTT                                                     <  SRR19973074.2363795/150‑1 (MQ=60)
                                                                                                    CATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAG                                                  <  SRR19973074.1523302/150‑1 (MQ=60)
                                                                                                    CATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAG                                                  <  SRR19973074.2135545/150‑1 (MQ=60)
                                                                                                    CATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAG                                                  <  SRR19973074.2355794/150‑1 (MQ=60)
                                                                                                     ATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGA                                                 >  SRR19973074.3386347/1‑150 (MQ=60)
                                                                                                       TGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATT                                               <  SRR19973074.977882/150‑1 (MQ=60)
                                                                                                         TTTTATATGCATTAGCATTAATTGCAAAAAATTAATTTATCATTCTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCT                                             >  SRR19973074.1145972/1‑150 (MQ=60)
                                                                                                            TATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCG                                          <  SRR19973074.4036511/150‑1 (MQ=60)
                                                                                                             ATATGCATTAGCACTAATTGCAAAAAATTATTTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGG                                         >  SRR19973074.3870098/1‑150 (MQ=60)
                                                                                                             ATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGG                                         >  SRR19973074.618436/1‑150 (MQ=60)
                                                                                                              TATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGG                                        >  SRR19973074.1280056/1‑150 (MQ=60)
                                                                                                              TATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGG                                        <  SRR19973074.859032/150‑1 (MQ=60)
                                                                                                               ATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGA                                       >  SRR19973074.2336833/1‑150 (MQ=60)
                                                                                                                TGCATTAGCACTAATTGCAAAAAATTAATTTATCATTCTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAG                                      <  SRR19973074.1822812/150‑1 (MQ=60)
                                                                                                                TGCATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAG                                      <  SRR19973074.1320267/150‑1 (MQ=60)
                                                                                                                  CATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTATCATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCA                                    >  SRR19973074.89426/1‑150 (MQ=60)
                                                                                                                  CATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCA                                    >  SRR19973074.89441/1‑150 (MQ=60)
                                                                                                                   ATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAA                                   <  SRR19973074.2833880/150‑1 (MQ=60)
                                                                                                                   ATTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAA                                   <  SRR19973074.2835502/150‑1 (MQ=60)
                                                                                                                    TTAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAAT                                  <  SRR19973074.1056964/150‑1 (MQ=60)
                                                                                                                     TAGCACTAATTGCAAAAAATTAATTTATCATTCTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATC                                 <  SRR19973074.2365131/150‑1 (MQ=60)
                                                                                                                     TAGCACTAATTGCAAAAAATTAATTTATCATTCTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATC                                 <  SRR19973074.2365045/150‑1 (MQ=60)
                                                                                                                     TAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATC                                 >  SRR19973074.1935865/1‑150 (MQ=60)
                                                                                                                     TAGCACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATC                                 <  SRR19973074.121829/150‑1 (MQ=60)
                                                                                                                         ACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTC                             <  SRR19973074.2802347/150‑1 (MQ=60)
                                                                                                                         ACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTC                             <  SRR19973074.2802479/150‑1 (MQ=60)
                                                                                                                         ACTAATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTC                             <  SRR19973074.3874278/150‑1 (MQ=60)
                                                                                                                             ATTGCAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTA                         <  SRR19973074.1712195/150‑1 (MQ=60)
                                                                                                                                 CAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTT                     <  SRR19973074.2331466/150‑1 (MQ=60)
                                                                                                                                 CAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTT                     >  SRR19973074.2034417/1‑150 (MQ=60)
                                                                                                                                 CAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTT                     <  SRR19973074.2331460/150‑1 (MQ=60)
                                                                                                                                 CAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTT                     <  SRR19973074.2720523/150‑1 (MQ=60)
                                                                                                                                 CAAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTT                     <  SRR19973074.2720556/150‑1 (MQ=60)
                                                                                                                                  AAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTG                    >  SRR19973074.1643346/1‑150 (MQ=60)
                                                                                                                                  AAAAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTG                    >  SRR19973074.1643322/1‑150 (MQ=60)
                                                                                                                                    AAAATTAATTTATCTTTATGTACACATATTTAGTACAAGTTTGCTATTGTTACTTCTCTTAAGATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCA                  <  SRR19973074.3493994/150‑1 (MQ=60)
                                                                                                                                    AAAATTAATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCA                  <  SRR19973074.3494012/150‑1 (MQ=60)
                                                                                                                                           ATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCAAAAGCTA           >  SRR19973074.2009071/1‑150 (MQ=60)
                                                                                                                                           ATTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCAAAAGCTA           <  SRR19973074.1055334/150‑1 (MQ=60)
                                                                                                                                            TTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCAAAAGCTAA          <  SRR19973074.2647290/150‑1 (MQ=60)
                                                                                                                                            TTTATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCAAAAGCTAA          <  SRR19973074.2743213/150‑1 (MQ=60)
                                                                                                                                              TATCATTATGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCAAAAGCTAACT        <  SRR19973074.2172840/150‑1 (MQ=60)
                                                                                                                                                    TATGTACACATATTTCGTACAAATTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCAAAAGCTAACTTGCATA  <  SRR19973074.4158435/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
TTTCTTTACATTGATAAAAAATGGAAAAAGTTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTTTTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATTCTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATGCATCAAAATTCAGTGACTTTAGATTCTGCGGGAGCAATCACTCGTTACTTTGCAAAAGCTAACTTGCATA  >  CP009273/1211020‑1211317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑