Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,575,339 G→T 100% F481L (TTC→TTA)  ydeN ← putative Ser‑type periplasmic non‑aryl sulfatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,575,3390GT97.3% 742.1 / 10.1 222F481L (TTC→TTA) ydeNputative Ser‑type periplasmic non‑aryl sulfatase
Reads supporting (aligned to +/- strand):  ref base G (5/1);  new base T (106/109);  total (112/110)
Fisher's exact test for biased strand distribution p-value = 2.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 223 total aligned reads displayed.

ACCACGCCTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGG  >  CP009273/1575193‑1575485
                                                                                                                                                  |                                                                                                                                                  
aCCACGCCTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAAt                                                                                                                                                 >  1:1227624/1‑150 (MQ=255)
  cACGCCTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTg                                                                                                                                               <  2:210690/150‑1 (MQ=255)
  cACGCCTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTg                                                                                                                                               <  2:4019844/150‑1 (MQ=255)
   aCGCCTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTgg                                                                                                                                              >  2:149175/1‑150 (MQ=255)
   aCGCCTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTgg                                                                                                                                              <  1:1610825/150‑1 (MQ=255)
    cGCCTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGc                                                                                                                                             <  2:290458/150‑1 (MQ=255)
      ccTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCtt                                                                                                                                           <  2:1867036/150‑1 (MQ=255)
      ccTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCtt                                                                                                                                           <  2:4138450/150‑1 (MQ=255)
       cTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTa                                                                                                                                          <  2:2372295/150‑1 (MQ=255)
        ttGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTaa                                                                                                                                         <  1:3486267/150‑1 (MQ=255)
         tGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAg                                                                                                                                        <  1:3182765/150‑1 (MQ=255)
          gCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGt                                                                                                                                       <  1:3807834/150‑1 (MQ=255)
           cATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTc                                                                                                                                      >  2:4047184/1‑150 (MQ=255)
             tctcTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCt                                                                                                                                    <  2:3406151/150‑1 (MQ=255)
             tctcTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCt                                                                                                                                    <  2:3469902/150‑1 (MQ=255)
               tctatcaCGACCTGCGGTTTGGCGGCGGCAATGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCa                                                                                                                                  <  1:2031451/144‑1 (MQ=255)
               tctTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTTTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCa                                                                                                                                  <  2:1789226/150‑1 (MQ=255)
                   tAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAgtgt                                                                                                                              >  2:3021438/1‑150 (MQ=255)
                         ccTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGt                                                                                                                        >  1:3061209/1‑150 (MQ=255)
                          cTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGtt                                                                                                                       >  1:3892625/1‑150 (MQ=255)
                              ggATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGc                                                                                                                   <  2:1895051/150‑1 (MQ=255)
                                aTTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCgg                                                                                                                 >  2:3978504/1‑150 (MQ=255)
                                  tGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGt                                                                                                               <  1:2158113/150‑1 (MQ=255)
                                       gcggcAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCg                                                                                                          <  1:1322320/150‑1 (MQ=255)
                                        aggcAAGGTTATCATTTAGCTGTATATCCGTCAGTTTGTAGAGACCTAACTGATTGAAATCTACTGTCTAGACAAGCGAATAATCTTTATTTATCACCGTAAAAGATAATTGGCAAACGACCTCCGTGTGGGGGTTAAGCGGGTAAACGt                                                                                                         >  2:2769687/2‑150 (MQ=255)
                                         ggcAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTc                                                                                                        >  1:628070/1‑150 (MQ=255)
                                          gcAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTct                                                                                                       <  2:3891565/150‑1 (MQ=255)
                                           cAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTctg                                                                                                      >  1:1284403/1‑150 (MQ=255)
                                                  aTCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATg                                                                                               <  1:924965/150‑1 (MQ=255)
                                                  aTCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATg                                                                                               <  1:3575150/150‑1 (MQ=255)
                                                  aTCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATg                                                                                               <  1:3477794/150‑1 (MQ=255)
                                                     tttttGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCg                                                                                            <  2:3944100/150‑1 (MQ=255)
                                                         tGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACa                                                                                        >  1:4018522/1‑150 (MQ=255)
                                                            tGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAAt                                                                                     <  2:2651103/150‑1 (MQ=255)
                                                            tGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAAt                                                                                     <  2:427949/150‑1 (MQ=255)
                                                               aGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTtg                                                                                  >  1:3326110/1‑150 (MQ=255)
                                                                gATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTtgt                                                                                 <  1:3104350/150‑1 (MQ=255)
                                                                  tCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTgg                                                                               >  2:1616861/1‑150 (MQ=255)
                                                                   ccGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGt                                                                              <  1:2948926/150‑1 (MQ=255)
                                                                   ccGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGt                                                                              <  1:2949422/150‑1 (MQ=255)
                                                                    cGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAGGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTa                                                                             >  1:3211516/1‑150 (MQ=255)
                                                                    cGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTa                                                                             <  1:197436/150‑1 (MQ=255)
                                                                    cGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTa                                                                             >  1:2889841/1‑150 (MQ=255)
                                                                     gTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGACCAAATTTGTGGTaa                                                                            >  2:1402608/1‑150 (MQ=255)
                                                                     gTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTaa                                                                            <  1:4119337/150‑1 (MQ=255)
                                                                      tCAGTTTGTAGAGACCTATCTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAAt                                                                           <  2:53929/150‑1 (MQ=255)
                                                                      tCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAAt                                                                           <  1:1814289/150‑1 (MQ=255)
                                                                        aGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTa                                                                         <  2:2848129/150‑1 (MQ=255)
                                                                        aGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTa                                                                         <  1:3750515/150‑1 (MQ=255)
                                                                          tttGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATc                                                                       <  2:2498949/150‑1 (MQ=255)
                                                                            tGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATccc                                                                     <  2:275512/150‑1 (MQ=255)
                                                                               agagACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGa                                                                  >  1:50500/1‑150 (MQ=255)
                                                                               agagACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGa                                                                  >  1:1769504/1‑150 (MQ=255)
                                                                                 agaCCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAAt                                                                >  1:1430269/1‑150 (MQ=255)
                                                                                  gaCCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATg                                                               <  1:1013808/150‑1 (MQ=255)
                                                                                  gaCCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATg                                                               >  1:2241187/1‑150 (MQ=255)
                                                                                       aaCTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCTGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAata                                                          >  1:3492728/1‑150 (MQ=255)
                                                                                        aCTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAatat                                                         >  2:2253793/1‑150 (MQ=255)
                                                                                         cTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATAtt                                                        >  2:739941/1‑150 (MQ=255)
                                                                                          tGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATAttt                                                       <  2:1400875/150‑1 (MQ=255)
                                                                                           gATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATAtttt                                                      <  1:502521/150‑1 (MQ=255)
                                                                                           gATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATAtttt                                                      >  1:2767786/1‑150 (MQ=255)
                                                                                           gATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATAtttt                                                      >  2:880559/1‑150 (MQ=255)
                                                                                           gATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATAtttt                                                      >  2:880793/1‑150 (MQ=255)
                                                                                            aTTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTc                                                     <  2:1933462/150‑1 (MQ=255)
                                                                                              tGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCt                                                   <  1:1986785/150‑1 (MQ=255)
                                                                                               gTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTc                                                  >  1:2375945/1‑150 (MQ=255)
                                                                                               gTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTc                                                  >  1:2707308/1‑150 (MQ=255)
                                                                                                 tttCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGt                                                >  1:892161/1‑150 (MQ=255)
                                                                                                 tttCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGt                                                >  2:1192811/1‑150 (MQ=255)
                                                                                                   tCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCa                                              >  1:3371272/1‑150 (MQ=255)
                                                                                                       cTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAAcc                                          <  2:1702859/150‑1 (MQ=255)
                                                                                                        tGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCa                                         >  1:785059/1‑150 (MQ=255)
                                                                                                        tGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCa                                         >  1:300298/1‑150 (MQ=255)
                                                                                                         gTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAg                                        >  2:3715551/1‑150 (MQ=255)
                                                                                                           ataGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATACTTTCCTCGTCAAACCAGTg                                      <  2:1006914/150‑1 (MQ=255)
                                                                                                            taGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTga                                     >  2:2300491/1‑150 (MQ=255)
                                                                                                            taGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTga                                     <  1:1327744/150‑1 (MQ=255)
                                                                                                                 aaGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATa                                >  2:2405949/1‑150 (MQ=255)
                                                                                                                 aaGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATa                                >  2:1858342/1‑150 (MQ=255)
                                                                                                                 aaGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATa                                >  1:2095564/1‑150 (MQ=255)
                                                                                                                      aataatCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAgg                           <  1:1692716/150‑1 (MQ=255)
                                                                                                                      aataatCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAgg                           >  2:3227890/1‑150 (MQ=255)
                                                                                                                       ataatCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGt                          <  1:1167879/150‑1 (MQ=255)
                                                                                                                        taatCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGtt                         <  1:1287740/150‑1 (MQ=255)
                                                                                                                         aatTGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTa                        >  1:2345143/1‑150 (MQ=255)
                                                                                                                          atCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTAt                       <  1:3176177/150‑1 (MQ=255)
                                                                                                                          atCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTAt                       >  1:2476951/1‑150 (MQ=255)
                                                                                                                             gTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCa                    <  2:1952820/150‑1 (MQ=255)
                                                                                                                                 tttCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTc                >  2:793926/1‑150 (MQ=255)
                                                                                                                                    ctcACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGa             <  2:2770187/150‑1 (MQ=255)
                                                                                                                                    ctcACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTGGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGa             <  2:63086/150‑1 (MQ=255)
                                                                                                                                     tcACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGAt            >  2:1350105/1‑150 (MQ=255)
                                                                                                                                      cACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGAtt           <  1:3441310/150‑1 (MQ=255)
                                                                                                                                         cGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGAttttt        >  2:2037961/1‑150 (MQ=255)
                                                                                                                                         cGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGAttttt        >  2:3829399/1‑150 (MQ=255)
                                                                                                                                            ataAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTAtg     <  2:3934642/150‑1 (MQ=255)
                                                                                                                                             taAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTAtgt    >  1:3062360/1‑150 (MQ=255)
                                                                                                                                             taAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTAtgt    <  2:3302940/150‑1 (MQ=255)
                                                                                                                                              aaGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTAtgtg   >  1:1797296/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ACCACGCCTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGG  >  CP009273/1575193‑1575485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCACGCCTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGC  >  CP009273/1575193‑1575486
                                                                                                                                                  |                                                                                                                                                   
ACCACGCCTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAAT                                                                                                                                                  >  SRR19973074.1229973/1‑150 (MQ=60)
ACCACGCCTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAAT                                                                                                                                                  >  SRR19973074.1679899/1‑150 (MQ=60)
  CACGCCTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTG                                                                                                                                                <  SRR19973074.314743/150‑1 (MQ=60)
  CACGCCTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTG                                                                                                                                                <  SRR19973074.1557976/150‑1 (MQ=60)
  CACGCCTTGCATATCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTG                                                                                                                                                <  SRR19973074.313089/150‑1 (MQ=60)
   ACGCCTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGG                                                                                                                                               <  SRR19973074.1613888/150‑1 (MQ=60)
    CGCCTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGC                                                                                                                                              >  SRR19973074.1306239/1‑150 (MQ=60)
     GCCTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCT                                                                                                                                             >  SRR19973074.1972110/1‑150 (MQ=60)
      CCTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTT                                                                                                                                            <  SRR19973074.502265/150‑1 (MQ=60)
       CTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTA                                                                                                                                           >  SRR19973074.171492/1‑150 (MQ=60)
        TTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAA                                                                                                                                          <  SRR19973074.3492662/150‑1 (MQ=60)
        TTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAA                                                                                                                                          <  SRR19973074.3492676/150‑1 (MQ=60)
         TGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAG                                                                                                                                         <  SRR19973074.3188628/150‑1 (MQ=60)
         TGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAG                                                                                                                                         <  SRR19973074.3188643/150‑1 (MQ=60)
          GCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGT                                                                                                                                        <  SRR19973074.3814796/150‑1 (MQ=60)
          GCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGT                                                                                                                                        <  SRR19973074.3814680/150‑1 (MQ=60)
            ATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCC                                                                                                                                      >  SRR19973074.3931373/1‑150 (MQ=60)
            ATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCC                                                                                                                                      <  SRR19973074.1817706/150‑1 (MQ=60)
               TCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCA                                                                                                                                   <  SRR19973074.1642222/150‑1 (MQ=60)
               TCTATCACGACCTGCGGTTTGGCGGCGGCAATGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCA                                                                                                                                   <  SRR19973074.2035290/150‑1 (MQ=60)
                         CCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGT                                                                                                                         >  SRR19973074.3066855/1‑150 (MQ=60)
                          CTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTT                                                                                                                        >  SRR19973074.3899757/1‑150 (MQ=60)
                          CTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTT                                                                                                                        >  SRR19973074.4013507/1‑150 (MQ=60)
                               GATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCG                                                                                                                   >  SRR19973074.221723/1‑150 (MQ=60)
                                  TGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGT                                                                                                                <  SRR19973074.2162172/150‑1 (MQ=60)
                                   GGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTA                                                                                                               <  SRR19973074.3358400/150‑1 (MQ=60)
                                    GCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAA                                                                                                              <  SRR19973074.3898616/150‑1 (MQ=60)
                                       GCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCG                                                                                                           <  SRR19973074.1324799/150‑1 (MQ=60)
                                         GGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTC                                                                                                         >  SRR19973074.629251/1‑150 (MQ=60)
                                         GGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTC                                                                                                         >  SRR19973074.1987156/1‑150 (MQ=60)
                                         GGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTC                                                                                                         >  SRR19973074.1987279/1‑150 (MQ=60)
                                         GGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTC                                                                                                         >  SRR19973074.628412/1‑150 (MQ=60)
                                           CAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTG                                                                                                       >  SRR19973074.1286845/1‑150 (MQ=60)
                                           CAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTG                                                                                                       >  SRR19973074.1286372/1‑150 (MQ=60)
                                            AAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGA                                                                                                      >  SRR19973074.348606/1‑150 (MQ=60)
                                            AAGGTTAACTTTATGCTGTAGATCCGTCAGTTTGTAGAGACCAAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGA                                                                                                      >  SRR19973074.2410028/1‑150 (MQ=60)
                                                  ATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATG                                                                                                <  SRR19973074.3484179/150‑1 (MQ=60)
                                                  ATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATG                                                                                                <  SRR19973074.926725/150‑1 (MQ=60)
                                                  ATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATG                                                                                                <  SRR19973074.4086762/150‑1 (MQ=60)
                                                  ATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATG                                                                                                <  SRR19973074.3581745/150‑1 (MQ=60)
                                                  ATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATG                                                                                                <  SRR19973074.3576960/150‑1 (MQ=60)
                                                     TTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATTGTCTGACTGATGGCG                                                                                             <  SRR19973074.140218/150‑1 (MQ=60)
                                                     TTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCG                                                                                             <  SRR19973074.1187244/150‑1 (MQ=60)
                                                     TTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCG                                                                                             <  SRR19973074.2300802/150‑1 (MQ=60)
                                                         TGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACA                                                                                         >  SRR19973074.4025951/1‑150 (MQ=60)
                                                         TGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACA                                                                                         >  SRR19973074.1799585/1‑150 (MQ=60)
                                                               AGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTG                                                                                   >  SRR19973074.3332286/1‑150 (MQ=60)
                                                               AGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTG                                                                                   >  SRR19973074.3332299/1‑150 (MQ=60)
                                                               AGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTG                                                                                   >  SRR19973074.1287954/1‑150 (MQ=60)
                                                                GATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGT                                                                                  <  SRR19973074.3110053/150‑1 (MQ=60)
                                                                   CCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGT                                                                               <  SRR19973074.2954905/150‑1 (MQ=60)
                                                                   CCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGT                                                                               <  SRR19973074.2954416/150‑1 (MQ=60)
                                                                   CCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGT                                                                               <  SRR19973074.2954407/150‑1 (MQ=60)
                                                                    CGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAGGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTA                                                                              >  SRR19973074.3217443/1‑150 (MQ=60)
                                                                    CGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTA                                                                              >  SRR19973074.2895204/1‑150 (MQ=60)
                                                                    CGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTA                                                                              <  SRR19973074.197854/150‑1 (MQ=60)
                                                                     GTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAA                                                                             <  SRR19973074.4126936/150‑1 (MQ=60)
                                                                     GTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAA                                                                             >  SRR19973074.2073617/1‑150 (MQ=60)
                                                                     GTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAA                                                                             <  SRR19973074.2160950/150‑1 (MQ=60)
                                                                      TCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAAT                                                                            <  SRR19973074.1817744/150‑1 (MQ=60)
                                                                        AGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTA                                                                          <  SRR19973074.3757410/150‑1 (MQ=60)
                                                                             GTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCA                                                                     >  SRR19973074.2859556/1‑150 (MQ=60)
                                                                               AGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGA                                                                   >  SRR19973074.50636/1‑150 (MQ=60)
                                                                               AGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGA                                                                   >  SRR19973074.1772822/1‑150 (MQ=60)
                                                                                GAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAA                                                                  <  SRR19973074.3327995/150‑1 (MQ=60)
                                                                                 AGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAAT                                                                 >  SRR19973074.1432920/1‑150 (MQ=60)
                                                                                  GACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATG                                                                >  SRR19973074.2245400/1‑150 (MQ=60)
                                                                                  GACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATG                                                                <  SRR19973074.1015691/150‑1 (MQ=60)
                                                                                      TAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAAT                                                            <  SRR19973074.2491713/150‑1 (MQ=60)
                                                                                       AACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCTGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATA                                                           >  SRR19973074.3499134/1‑150 (MQ=60)
                                                                                           GATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTT                                                       <  SRR19973074.503530/150‑1 (MQ=60)
                                                                                           GATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTT                                                       <  SRR19973074.503529/150‑1 (MQ=60)
                                                                                           GATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTT                                                       >  SRR19973074.2772789/1‑150 (MQ=60)
                                                                                           GATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTT                                                       >  SRR19973074.2772872/1‑150 (MQ=60)
                                                                                            ATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTC                                                      <  SRR19973074.2642836/150‑1 (MQ=60)
                                                                                              TGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCT                                                    <  SRR19973074.1990563/150‑1 (MQ=60)
                                                                                              TGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCT                                                    <  SRR19973074.1990590/150‑1 (MQ=60)
                                                                                               GTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTC                                                   <  SRR19973074.658069/150‑1 (MQ=60)
                                                                                               GTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTC                                                   >  SRR19973074.2380362/1‑150 (MQ=60)
                                                                                               GTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTC                                                   >  SRR19973074.2712326/1‑150 (MQ=60)
                                                                                                TTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCG                                                  >  SRR19973074.536525/1‑150 (MQ=60)
                                                                                                 TTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGT                                                 >  SRR19973074.893866/1‑150 (MQ=60)
                                                                                                   TCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCA                                               >  SRR19973074.3377521/1‑150 (MQ=60)
                                                                                                       CTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACC                                           >  SRR19973074.3005231/1‑150 (MQ=60)
                                                                                                        TGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCA                                          >  SRR19973074.1325824/1‑150 (MQ=60)
                                                                                                        TGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCA                                          >  SRR19973074.786571/1‑150 (MQ=60)
                                                                                                        TGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCA                                          >  SRR19973074.300860/1‑150 (MQ=60)
                                                                                                        TGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCA                                          >  SRR19973074.786626/1‑150 (MQ=60)
                                                                                                           ATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTG                                       <  SRR19973074.3002674/150‑1 (MQ=60)
                                                                                                            TAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGA                                      <  SRR19973074.1330232/150‑1 (MQ=60)
                                                                                                                CAAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAAT                                  >  SRR19973074.2362780/1‑150 (MQ=60)
                                                                                                                 AAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATA                                 >  SRR19973074.3091785/1‑150 (MQ=60)
                                                                                                                 AAGCGAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATA                                 >  SRR19973074.2099486/1‑150 (MQ=60)
                                                                                                                     GAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAG                             >  SRR19973074.3675737/1‑150 (MQ=60)
                                                                                                                     GAATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAG                             >  SRR19973074.3416964/1‑150 (MQ=60)
                                                                                                                      AATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGG                            <  SRR19973074.1695879/150‑1 (MQ=60)
                                                                                                                       ATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGT                           <  SRR19973074.1170098/150‑1 (MQ=60)
                                                                                                                       ATAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGT                           >  SRR19973074.3576492/1‑150 (MQ=60)
                                                                                                                        TAATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTT                          <  SRR19973074.1290184/150‑1 (MQ=60)
                                                                                                                         AATTGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTA                         >  SRR19973074.2349525/1‑150 (MQ=60)
                                                                                                                         AATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTA                         >  SRR19973074.2172137/1‑150 (MQ=60)
                                                                                                                          ATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTAT                        <  SRR19973074.3182023/150‑1 (MQ=60)
                                                                                                                          ATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTAT                        >  SRR19973074.2481547/1‑150 (MQ=60)
                                                                                                                          ATCGTTATTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTAT                        <  SRR19973074.3182024/150‑1 (MQ=60)
                                                                                                                                 TTTCTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTC                 >  SRR19973074.930191/1‑150 (MQ=60)
                                                                                                                                    CTCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGA              >  SRR19973074.902114/1‑150 (MQ=60)
                                                                                                                                     TCACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGAT             <  SRR19973074.3047052/150‑1 (MQ=60)
                                                                                                                                      CACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATT            <  SRR19973074.3448330/150‑1 (MQ=60)
                                                                                                                                      CACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATT            <  SRR19973074.3447645/150‑1 (MQ=60)
                                                                                                                                       ACCGTATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTATTTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTT           <  SRR19973074.2900407/150‑1 (MQ=60)
                                                                                                                                           TATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTAT       <  SRR19973074.1290611/150‑1 (MQ=60)
                                                                                                                                            ATAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATG      >  SRR19973074.1025427/1‑150 (MQ=60)
                                                                                                                                             TAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGT     >  SRR19973074.3068007/1‑150 (MQ=60)
                                                                                                                                             TAAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGT     >  SRR19973074.3067980/1‑150 (MQ=60)
                                                                                                                                              AAGATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTG    >  SRR19973074.1800711/1‑150 (MQ=60)
                                                                                                                                                GATAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGC  <  SRR19973074.1507244/150‑1 (MQ=60)
                                                                                                                                                  |                                                                                                                                                   
ACCACGCCTTGCATCTCTTTAACGACCTGCGGATTGGCGGCGGCAAGGTTATCTTTTTGCTGTAGATCCGTCAGTTTGTAGAGACCTAACTGATTGTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGC  >  CP009273/1575193‑1575486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑