Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F15 I1 R1	782	634.5	21863942	98.1%	21448527	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,219,333	G→A	intergenic (‑230/+27)	iraM ← / ← CXP41_RS24360	anti‑adapter protein IraM/protein YmgK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,219,333	0	G	A	100.0%	124.9 / NA	34	intergenic (‑230/+27)	iraM/CXP41_RS24360	anti‑adapter protein IraM/protein YmgK
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/17); total (17/17)

AATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATGCATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGCATGACATGA  >  NZ_CP025268/1219200‑1219454

aattaattAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCa                                                                                                                     <  1:2860152/140‑1 (MQ=255)

               tgGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATcc                                                                                                      >  2:1009027/1‑140 (MQ=255)

                      tataAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAAtt                                                                                               <  1:8457976/140‑1 (MQ=255)

                                aatTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGc                                                                                     <  1:1130536/140‑1 (MQ=255)

                                aatTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGc                                                                                     >  2:10704776/1‑140 (MQ=255)

                                       aGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATg                                                                              >  2:4939783/1‑140 (MQ=255)

                                            ggAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACAt                                                                                        >  2:6963941/1‑125 (MQ=255)

                                            ggAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACAt                                                                                        <  1:6963941/125‑1 (MQ=255)

                                                      tGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGa                                                                                                            >  2:10549601/1‑95 (MQ=255)

                                                      tGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGa                                                                                                            <  1:10549601/95‑1 (MQ=255)

                                                           gTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCt                                                          <  1:6517261/140‑1 (MQ=255)

                                                            ttAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTa                                                         <  1:7147198/140‑1 (MQ=255)

                                                            ttAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTa                                                         <  1:7147342/140‑1 (MQ=255)

                                                                    aaTGTGAATTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGAGTTGTTTAGATATAATGTTATACATTCAAATGACTGAACAGCCTGTAAGTAAAACTTAGAGTTTATGCTACTTTGTGGCACTTTGCTTATCAGTAt                                                 >  2:7421800/1‑140 (MQ=255)

                                                                    aaTGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTag                                                 >  1:2778043/1‑139 (MQ=255)

                                                                    aaTGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTAt                                                 >  2:7421788/1‑140 (MQ=255)

                                                                    aaTGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACAGTAt                                                 >  1:366787/1‑140 (MQ=255)

                                                                         caTTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTg                                            <  2:10677995/139‑1 (MQ=255)

                                                                              tgtgAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACa                                                                                         <  1:3057638/90‑1 (MQ=255)

                                                                              tgtgAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACa                                                                                         >  2:3057638/1‑90 (MQ=255)

                                                                              tgtgAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCttt                                       >  2:8856804/1‑140 (MQ=255)

                                                                                    aGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTtatat                                 <  1:10163979/140‑1 (MQ=255)

                                                                                              tattGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGataata                       <  1:10415773/140‑1 (MQ=255)

                                                                                               attGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGAtaataa                      <  1:10704776/140‑1 (MQ=255)

                                                                                                 tGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATg                    >  1:1114654/1‑140 (MQ=255)

                                                                                                     ttCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAata                >  1:3835333/1‑140 (MQ=255)

                                                                                                      tCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAatat               >  2:3286707/1‑140 (MQ=255)

                                                                                                       cGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAAtata              >  2:2306730/1‑140 (MQ=255)

                                                                                                                tttGTTTAGCTATAATGTTATACATTCAAATGAATGATCATCCTGTAATTAAAACATAGCATTTATTCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGTTAATAATGAATATATGcatgaca     <  2:6065062/140‑1 (MQ=255)

                                                                                                                tttGTTTAGCTATAATGTTATACAATCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGcatgaca     <  2:6064969/140‑1 (MQ=255)

                                                                                                                 ttGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGcatgacat    <  1:2306730/140‑1 (MQ=255)

                                                                                                                 ttGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGcatgacat    <  1:1009027/140‑1 (MQ=255)

                                                                                                                   gTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGcatgacatga  >  1:7297850/1‑140 (MQ=255)

                                                                                                                   gTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGcatgacatga  >  1:1036341/1‑140 (MQ=255)

AATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATGCATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGCATGACATGA  >  NZ_CP025268/1219200‑1219454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATGCATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTAT  >  NZ_CP025268/1219192‑1219480

agaagccggcatacgagattcgccttagtctcgtgggctcggagatgtgtataagagacagATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAAT                                                                                                                                            <  SRR14920162.8588848/90‑1 (MQ=60)

 AAAAATAACTTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATG                                                                                                                                           <  SRR14920162.2298109/151‑1 (MQ=60)

 AAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATG                                                                                                                                           <  SRR14920162.2298165/151‑1 (MQ=60)

        AATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAAC                                                                                                                                    <  SRR14920162.2877024/151‑1 (MQ=60)

                 gtctcgtgggctcggagatgtgtataagagacagAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAA                                                                                                                           <  SRR14920162.10614382/117‑1 (MQ=60)

                              TATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCC                                                                                                              <  SRR14920162.8509364/151‑1 (MQ=60)

                                    ccttagtctcgtgggctcggagatgtgtataagagacagAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATG                                                                                                        <  SRR14920162.3075817/112‑1 (MQ=60)

                                     acagATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGC                                                                                                       <  SRR14920162.7005790/147‑1 (MQ=60)

                                        AATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTAC                                                                                                    <  SRR14920162.1137167/151‑1 (MQ=60)

                                                              ccctttccacagcccacgccgccatacgagattcgccttagtctcgtgggctcggagatgtgtataagagacagGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACAT                                                                              <  SRR14920162.5514860/77‑1 (MQ=60)

                                                              ccactttacacgcataagacgtaacacgagattcgccttagtctcgtgggctcggagatgtgtataagagacagGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACAT                                                                              <  SRR14920162.6102913/77‑1 (MQ=60)

                                                                 ATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTAG                                                                           >  SRR14920162.2794433/1‑151 (MQ=60)

                                                                 ATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACAGTAT                                                                           >  SRR14920162.369098/1‑151 (MQ=60)

                                                                   GTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGG                                                                         <  SRR14920162.6556141/151‑1 (MQ=60)

                                                                    TTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGT                                                                        <  SRR14920162.7190358/151‑1 (MQ=60)

                                                                    TTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGT                                                                        <  SRR14920162.7190213/151‑1 (MQ=60)

                                                                       gacggcatccgagattcgccttagtctcgtgggctcggagatgtgtataagagacagGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGC                                                                     <  SRR14920162.5668832/94‑1 (MQ=60)

                                                                                            AGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAA                                                <  SRR14920162.10225953/151‑1 (MQ=60)

                                                                                              CAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATG                                              >  SRR14920162.1121199/1‑151 (MQ=60)

                                                                                                  AAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATA                                          >  SRR14920162.3858000/1‑151 (MQ=60)

                                                                                                      TATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAG                                      <  SRR14920162.10479620/151‑1 (MQ=60)

                                                                                                       tcgtgggctcggagatgtgtataagagacagATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGC                                     <  SRR14920162.1313839/120‑1 (MQ=60)

                                                                                                       ATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGC                                     <  SRR14920162.10770677/151‑1 (MQ=60)

                                                                                                                GTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGCATGACATGA                            >  SRR14920162.7341803/1‑151 (MQ=60)

                                                                                                                GTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGCATGACATGA                            >  SRR14920162.1042430/1‑151 (MQ=60)

                                                                                                                         TTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGT                   <  SRR14920162.1014958/151‑1 (MQ=60)

                                                                                                                         TTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGT                   <  SRR14920162.2320297/151‑1 (MQ=60)

                                                                                                                                GCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCctgtctcttatacacatctccgagcccacgagactaaggcgaatctcgtttgccggagtgtgggtgtaaattgggggggggggggggggggggggggg            >  SRR14920162.6750448/1‑53 (MQ=60)

                                                                                                                                GCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCctgtctcttatacacatctccgagcccacgagactaaggcgaatctcggatgccgggttgtgcttgtaaagggggggggggggggggggggggggggg            >  SRR14920162.10493110/1‑53 (MQ=60)

                                                                                                                                GCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACtgtctcttatacacatctccgagcccacgagactaaggcgaatc            >  SRR14920162.10431158/1‑107 (MQ=60)

                                                                                                                                GCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACtgtctcttatacacatctccgagcccacgagactaaggcgaatc            >  SRR14920162.10431049/1‑107 (MQ=60)

                                                                                                                                GCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAActgtctcttatac            >  SRR14920162.6345134/1‑138 (MQ=60)

                                                                                                                                   ATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTctgtctcttatacacatctccgagcccacgagactaaggcgaatctcggtgtgcgtcgtgtgcgtggaagtgggggggggggggggggg         >  SRR14920162.2124592/1‑62 (MQ=60)

                                                                                                                                      ATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCtgtctcttatacacatctccgagcccacgagactaaggcgaatctcgggttgcggcgggtggttgtggatgggggggggggggggg      >  SRR14920162.2163003/1‑65 (MQ=60)

                                                                                                                                      ATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTctgtctcttatacacatctccgagcccacgagactaaggcgaatctcggttgccggcgtg      >  SRR14920162.3816428/1‑91 (MQ=60)

                                                                                                                                      ATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATctgtctcttatacacatctccgagcccacgagactaaggcgaatctcggatgg      >  SRR14920162.9859253/1‑98 (MQ=60)

                                                                                                                                      ATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAActgtctcttatacacatctccgagcccacgagactaaggcga      >  SRR14920162.9953931/1‑109 (MQ=60)

                                                                                                                                      ATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGCATGACATGAGctgtctcttatacacatctcc      >  SRR14920162.729619/1‑130 (MQ=60)

                                                                                                                                      ATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGCATGACATGAGctgtctcttatacacatctcc      >  SRR14920162.730907/1‑130 (MQ=60)

                                                                                                                                      ATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGCATGACATGAGctgtctcttatacacatctcc      >  SRR14920162.731602/1‑130 (MQ=60)

                                                                                                                                      ATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAG      >  SRR14920162.6899761/1‑151 (MQ=60)

                                                                                                                                      ATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAG      >  SRR14920162.4148521/1‑151 (MQ=60)

                                                                                                                                      ATGTTAAACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTTCCAATTTGCTAAACATTATGGTTGCCTTTTTATATACCGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAG      >  SRR14920162.7764064/1‑151 (MQ=60)

                                                                                                                                        GTTATACATTCAAATGACTGAACATCCTGTAATTAAActgtctcttatacacatctccgagcccacgagactaaggcgaatctcgtgtgccggcttgtgggtgtaaatgtggggggggggggggggggggggggggggggggggggggggg    >  SRR14920162.9792087/1‑37 (MQ=60)

                                                                                                                                        GTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTT    >  SRR14920162.5154547/1‑151 (MQ=60)

                                                                                                                                          TATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTAT  >  SRR14920162.4138903/1‑151 (MQ=60)

                                                                                                                                          TATACATACAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTATTATATAACGATAATCATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTAT  >  SRR14920162.4139587/1‑151 (MQ=60)

AAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATGCATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTTTTATATAACGATAATAATGAATATAAGCATGACATGAGAATAAGGTTTCAATTTTTGAGTTAT  >  NZ_CP025268/1219192‑1219480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`