breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 49,765 C→T intergenic (+134/‑58) kefC → / → folA glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase
RA 49,910 T→G W30G (TGG→GGG)  folA → type 3 dihydrofolate reductase
RA 50,279 T→G F153V (TTT→GTT)  folA → type 3 dihydrofolate reductase
RA 59,627 C→A intergenic (‑153/+60) BW25113_RS25400 ← / ← rluA transposase/bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA
RA 1,314,400 C→T A414A (GCG→GCA trpD ← bifunctional anthranilate synthase glutamate amidotransferase component TrpG/anthranilate phosphoribosyltransferase TrpD
RA 1,393,311 A→G I158T (ATC→ACC)  fnr ← fumarate/nitrate reduction transcriptional regulator Fnr
RA 1,613,588 T→C L71P (CTG→CCG)  marR → multiple antibiotic resistance transcriptional regulator MarR
MC JC 2,399,465 Δ7 bp coding (650‑656/939 nt) lrhA ← transcriptional regulator LrhA
RA 4,062,410 G→T P76Q (CCG→CAG)  yihQ ← sulfoquinovosidase
RA 4,158,126 T→C noncoding (1540/1542 nt) BW25113_RS20590 → 16S ribosomal RNA
RA 4,210,609 T→G T603P (ACG→CCG)  espL4 ← type III secretion system effector EspL4

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 356981 357569 589 12 [0] [2] 6 lacA galactoside O‑acetyltransferase
* * ÷ NZ_CP009273 982686 982747 62 4 [2] [2] 3 ompF/asnS porin OmpF/asparagine‑‑tRNA ligase

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 360104 =78 (2.520)102 (3.290) 47/242 0.0 56.7% intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 NA (NA)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 1203246 =10 (0.320)20 (0.740) 16/210 0.5 67.0% coding (290/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 1205075 = 11 (0.410)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 120326110 (0.320)14 (0.520) 13/210 0.7 58.7% coding (305/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 = 1205058 11 (0.410)coding (57/540 nt) BW25113_RS06005 phage tail protein