breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 67 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,621,9840AC66.7% ‑5.3 / 15.5 22V105G (GTC→GGC) pdeFcyclic‑guanylate‑specific phosphodiesterase PdeF
*NZ_CP0092731,976,0000TG64.3% 5.3 / 11.3 14intergenic (‑132/+35)otsB/araHtrehalose‑phosphatase/arabinose ABC transporter permease AraH
*NZ_CP009273536,0140TG64.0% 9.0 / 24.7 25intergenic (+50/‑7)allB/ybbYallantoinase AllB/uracil/xanthine transporter
*NZ_CP009273760,1870GC61.1% 3.8 / 21.7 18P184P (CCG→CCCsucDsuccinate‑‑CoA ligase subunit alpha
*NZ_CP0092734,594,1610CT57.5% 5.2 / 47.9 40S62F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NZ_CP009273807,1770TG56.5% 7.5 / 21.0 23V67G (GTG→GGG) bioCmalonyl‑ACP O‑methyltransferase BioC
*NZ_CP0092731,377,9910AC55.6% 14.0 / 17.6 18G76G (GGT→GGGycjWLacI family DNA‑binding transcriptional regulator
*NZ_CP0092732,089,5710AG55.6% 7.7 / 22.3 18I83V (ATT→GTT) hisFimidazole glycerol phosphate synthase subunit HisF
*NZ_CP0092734,213,5210TG55.6% 30.3 / 37.1 27K12N (AAA→AACiclRglyoxylate bypass operon transcriptional repressor IclR
*NZ_CP0092734,213,5250CT55.6% 3.3 / 33.5 27R11K (AGA→AAA) iclRglyoxylate bypass operon transcriptional repressor IclR
*NZ_CP009273276,8000AC55.0% 20.2 / 26.2 20Y299D (TAT→GAT) yagAIS481‑like element ISEc19 family transposase
*NZ_CP009273170,5520AC54.5% 19.9 / 27.0 23V273G (GTA→GGA) hemLglutamate‑1‑semialdehyde 2,1‑aminomutase
*NZ_CP009273883,4880TA54.5% 6.4 / 24.1 22intergenic (+73/+102)rcdA/ybjLDNA‑binding transcriptional regulator RcdA/aspartate:alanine antiporter
*NZ_CP009273937,1690TG54.5% 8.6 / 29.1 22V252G (GTG→GGG) dmsAdimethylsulfoxide reductase subunit A
*NZ_CP0092731,975,9940CG53.8% 10.0 / 15.5 14intergenic (‑126/+41)otsB/araHtrehalose‑phosphatase/arabinose ABC transporter permease AraH
*NZ_CP0092734,227,1780TC53.8% 4.4 / 11.7 13D118D (GAT→GACyjbGcapsule biosynthesis GfcC family protein
*NZ_CP0092733,781,3850TG53.6% 14.5 / 28.6 28G396G (GGT→GGGenvCmurein hydrolase activator EnvC
*NZ_CP009273997,4470TG53.3% 12.2 / 19.2 15V62G (GTA→GGA) elfGfimbrial protein
*NZ_CP009273982,4700AC52.9% 12.6 / 20.1 17intergenic (‑32/+571)ompF/asnSporin OmpF/asparagine‑‑tRNA ligase
*NZ_CP0092731,540,6830AC52.9% 11.1 / 17.0 17G248G (GGT→GGGyddGaromatic amino acid efflux DMT transporter YddG

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 = 270718825 (0.840)3 (0.120) 3/146 2.2 10.6% coding (934/1335 nt) srmB ATP‑dependent RNA helicase SrmB
?NZ_CP009273 = 2707267 29 (1.120)coding (1013/1335 nt) srmB ATP‑dependent RNA helicase SrmB
* ? NZ_CP009273 2006338 =21 (0.700)3 (0.110) 3/148 2.2 14.5% coding (158/315 nt) fliE flagellar hook‑basal body complex protein FliE
?NZ_CP009273 2006392 = 17 (0.640)coding (104/315 nt) fliE flagellar hook‑basal body complex protein FliE
* ? NZ_CP009273 = 450564718 (0.600)3 (0.100) 3/162 2.4 14.5% coding (848/2325 nt) fecA Fe(3+) dicitrate transport protein FecA
?NZ_CP009273 4505651 = 18 (0.620)coding (844/2325 nt) fecA Fe(3+) dicitrate transport protein FecA
* ? NZ_CP009273 1955597 =25 (0.840)3 (0.100) 3/168 2.4 7.7% coding (1279/1734 nt) argS arginine‑‑tRNA ligase
?NZ_CP009273 1955639 = 47 (1.570)coding (1321/1734 nt) argS arginine‑‑tRNA ligase