breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 77 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM11,327,1210CT51.5% 763.3 / inf 1639intergenic (+97/‑196)PP_16SE rRNA (16S ribosomal RNA)/PP_23SE rRNA (23S ribosomal RNA)–/–
*AE015451_phaM14,348,7050AG50.4% 3.6 / inf 1492intergenic (‑70/+404)PP_3820 CDS (Group II intron‑encoding maturase)/galU CDS (UTP‑glucose‑1‑phosphate uridylyltransferase)–/–
*AE015451_phaM1699,7220AG49.6% 18.6 / inf 959intergenic (+115/‑129)PP_t06 tRNA (tRNA‑Ala)/PP_23SD rRNA (23S ribosomal RNA)–/–
*AE015451_phaM14,675,3600TG47.1% 210.7 / 76.5 246intergenic (+59/+45)PP_4137 CDS (putative Outer membrane siderophore receptor)/chrR CDS (Chromate reductase)–/–
*AE015451_phaM14,344,3120GA36.9% 45.0 / 38.9 109intergenic (+43/‑113)PP_3817 CDS (Polyamine ABC transporter_ ATP‑binding protein)/PP_3818 CDS (Periplamic phosphate‑binding protein)–/–
*AE015451_phaM15,737,7560AG36.8% 281.6 / 101.1 253intergenic (+45/+26)hutF CDS (probable formiminoglutamate deiminase)/PP_5037 CDS (lipocalin family lipoprotein)–/–
*AE015451_phaM1195,6340CT36.5% 1623.4 / inf 1241T380T (ACC→ACTPP_0168 CDS (putative surface adhesion protein)
*AE015451_phaM14,889,6230AG36.1% 189.3 / 25.8 135intergenic (+69/‑84)hyi CDS (hydroxypyruvate isomerase)/glxR CDS (tartronate semialdehyde reductase)–/–
*AE015451_phaM14,344,3080GA35.8% 101.0 / 31.6 123intergenic (+39/‑117)PP_3817 CDS (Polyamine ABC transporter_ ATP‑binding protein)/PP_3818 CDS (Periplamic phosphate‑binding protein)–/–
*AE015451_phaM14,889,6440GC34.2% 405.6 / 124.3 228intergenic (+90/‑63)hyi CDS (hydroxypyruvate isomerase)/glxR CDS (tartronate semialdehyde reductase)–/–
*AE015451_phaM15,223,0130CA34.1% ‑1.4 / 19.0 44L428F (TTG→TTTPP_4603 CDS (transposase)
*AE015451_phaM12,178,5680GA33.1% 1961.8 / inf 1167T268T (ACG→ACAPP_1931 CDS (conserved protein of unknown function)
*AE015451_phaM11,210,8300GT29.8% 1235.5 / 277.0 611intergenic (+20/+17)ytnA CDS (Uncharacterized amino acid permease YtnA)/PP_1060 CDS (putative Glutamate synthase_ large subunit)–/–
*AE015451_phaM11,210,8230CG29.6% 1272.3 / 244.0 601intergenic (+13/+24)ytnA CDS (Uncharacterized amino acid permease YtnA)/PP_1060 CDS (putative Glutamate synthase_ large subunit)–/–
*AE015451_phaM1614,2850AC29.6% 374.6 / 44.9 245intergenic (‑104/+74)dxs CDS (1‑deoxyxylulose‑5‑phosphate synthase)/ispA CDS (farnesyl diphosphate synthase)–/–
*AE015451_phaM11,210,8490AC29.3% 1001.5 / 108.5 556*540G (TGA→GGA) PP_1060 CDS (putative Glutamate synthase_ large subunit)
*AE015451_phaM14,675,3360TG29.1% 229.3 / 17.5 186intergenic (+35/+69)PP_4137 CDS (putative Outer membrane siderophore receptor)/chrR CDS (Chromate reductase)–/–
*AE015451_phaM12,241,3050TG28.9% 414.3 / 55.3 308intergenic (+52/‑229)gltX CDS (Glutamate‑‑tRNA ligase)/PP_t25 tRNA (tRNA‑Ala)–/–
*AE015451_phaM14,344,3230GA28.7% 256.9 / 18.7 145intergenic (+54/‑102)PP_3817 CDS (Polyamine ABC transporter_ ATP‑binding protein)/PP_3818 CDS (Periplamic phosphate‑binding protein)–/–
*AE015451_phaM14,353,1930AC28.4% 263.6 / 34.9 226T107P (ACG→CCG) yrpB CDS (2‑nitropropane dioxygenase)

Marginal new junction evidence (lowest skew 10 of 232 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AE015451_phaM1 = 1780066233 (0.270)176 (0.220) 69/256 3.9 43.6% intergenic (+25/+81) PP_1587 CDS (NhaP‑type Na+_H+ and K+_H+ antiporters)/dapC CDS (N‑succinyl‑L_L‑diaminopimelate aminotransferase alternative) –/–
?AE015451_phaM1 1780129 = 234 (0.290)intergenic (+88/+18) PP_1587 CDS (NhaP‑type Na+_H+ and K+_H+ antiporters)/dapC CDS (N‑succinyl‑L_L‑diaminopimelate aminotransferase alternative) –/–
* ? AE015451_phaM1 = 5699642859 (1.010)3109 (0.990) 65/248 4.1 88.7% intergenic (+388/‑3617) PP_5002 CDS (conserved protein of unknown function)/PP_5009 CDS (conserved protein of unknown function) –/–
?pS4413_qbsFH 12 = 0 (0.000)intergenic (–/‑1941) –/aadA CDS –/–
* ? AE015451_phaM1 = 555560847 (0.060)272 (0.330)
+GCC
56/264 6.2 85.5% intergenic (‑84/+87) PP_4887 CDS (conserved protein of unknown function)/PP_4888 CDS (putative methyl‑accepting chemotaxis transducer) –/–
?AE015451_phaM1 5555609 = 47 (0.060)intergenic (‑85/+86) PP_4887 CDS (conserved protein of unknown function)/PP_4888 CDS (putative methyl‑accepting chemotaxis transducer) –/–
* ? AE015451_phaM1 = 56814151 (0.000)245 (0.300)
+CGGG
46/262 8.2 99.6% intergenic (‑133/+71) PP_4986 CDS (Channel protein_ hemolysin III family)/PP_4987 CDS (putative Chemotaxis protein) –/–
?AE015451_phaM1 5681416 = 1 (0.000)intergenic (‑134/+70) PP_4986 CDS (Channel protein_ hemolysin III family)/PP_4987 CDS (putative Chemotaxis protein) –/–
* ? AE015451_phaM1 4808174 =56 (0.070)225 (0.360) 31/200 8.6 50.7% intergenic (‑283/‑272) PP_4234 CDS (Aldehyde oxidase and xanthine dehydrogenase)/dsbD‑II CDS (thiol_disulfide interchange protein 2) –/–
?AE015451_phaM1 = 5986211 396 (0.630)intergenic (+229/‑317) kefB‑III CDS (glutathione‑regulated potassium_H+ antiporter)/PP_mr64 ncRNA (C4 ncRNA) –/–
* ? AE015451_phaM1 = 2472115283 (0.330)113 (0.140) 40/256 9.3 23.3% intergenic (+19/+130) PP_2166 CDS (Anti‑anti‑sigma factor)/PP_2167 CDS (putative PhoD‑like phosphatase) –/–
?AE015451_phaM1 2472313 = 475 (0.590)coding (1783/1851 nt) PP_2167 CDS (putative PhoD‑like phosphatase)
* ? AE015451_phaM1 = 652782609 (0.710)124 (0.170) 30/230 10.8 32.3% coding (1961/1962 nt) ctpL CDS (Methyl‑accepting chemotaxis protein CtpL)
?AE015451_phaM1 = 4808159 0 (0.000)intergenic (‑268/‑287) PP_4234 CDS (Aldehyde oxidase and xanthine dehydrogenase)/dsbD‑II CDS (thiol_disulfide interchange protein 2) –/–
* ? AE015451_phaM1 2478409 =891 (1.040)52 (0.080) 26/218 11.4 6.8% coding (106/444 nt) PP_2172 CDS (conserved exported protein of unknown function)
?AE015451_phaM1 = 2478405 705 (1.020)coding (102/444 nt) PP_2172 CDS (conserved exported protein of unknown function)
* ? AE015451_phaM1 = 480817925 (0.030)108 (0.150) 25/230 12.5 31.0% intergenic (‑288/‑267) PP_4234 CDS (Aldehyde oxidase and xanthine dehydrogenase)/dsbD‑II CDS (thiol_disulfide interchange protein 2) –/–
?AE015451_phaM1 = 5986225 460 (0.630)intergenic (+243/‑303) kefB‑III CDS (glutathione‑regulated potassium_H+ antiporter)/PP_mr64 ncRNA (C4 ncRNA) –/–
* ? AE015451_phaM1 = 480817925 (0.030)110 (0.140) 27/246 12.7 30.6% intergenic (‑288/‑267) PP_4234 CDS (Aldehyde oxidase and xanthine dehydrogenase)/dsbD‑II CDS (thiol_disulfide interchange protein 2) –/–
?AE015451_phaM1 5986041 = 475 (0.610)intergenic (+59/‑487) kefB‑III CDS (glutathione‑regulated potassium_H+ antiporter)/PP_mr64 ncRNA (C4 ncRNA) –/–