breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 25 shown, sorted by frequency from high to low) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | AP012306 | 1,091,992 | 0 | T | G | 38.3% | 449.9 / 62.1 | 269 | intergenic (+38/‑145) | narI/ychS | nitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein |
* | AP012306 | 644,417 | 0 | G | A | 33.1% | 711.1 / inf | 525 | intergenic (+32/‑401) | lysQ/nadA | tRNA‑Lys/quinolinate synthase, subunit A |
* | AP012306 | 3,182,861 | 0 | A | C | 31.0% | 336.5 / 39.3 | 155 | L198F (TTA→TTC) | yiaN | predicted transporter |
* | AP012306 | 1,226,557 | 0 | A | G | 30.3% | 374.1 / 125.1 | 211 | G55G (GGA→GGG) | ydcU | predicted spermidine/putrescine transporter subunit |
* | AP012306 | 1,944,288 | 0 | A | C | 30.0% | 559.0 / 37.5 | 250 | G361G (GGT→GGG) | menE | o‑succinylbenzoate‑CoA ligase |
* | AP012306 | 3,938,887 | 0 | C | T | 27.7% | 492.4 / 137.1 | 242 | S44F (TCT→TTT) | bglJ | DNA‑binding transcriptional regulator |
* | AP012306 | 3,527,368 | 0 | A | C | 27.4% | 639.5 / 59.1 | 270 | L140F (TTA→TTC) | rhaS | DNA‑binding transcriptional activator, L‑rhamnose‑binding |
* | AP012306 | 800,675 | 0 | A | C | 26.6% | 668.3 / 27.4 | 290 | T192P (ACC→CCC) | lolA | chaperone for lipoproteins |
* | AP012306 | 341,377 | 0 | T | G | 26.3% | 256.0 / 15.6 | 137 | G60G (GGT→GGG) | ribD | fused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase |
* | AP012306 | 3,590,367 | 0 | A | C | 25.0% | 745.4 / 36.1 | 284 | T30P (ACC→CCC) | fabR | DNA‑binding transcriptional repressor |
* | AP012306 | 1,854,851 | 0 | A | C | 24.6% | 711.6 / 68.1 | 284 | T25P (ACC→CCC) | yejH | predicted ATP‑dependet helicase |
* | AP012306 | 1,834,247 | 0 | A | C | 23.5% | 457.5 / 13.6 | 183 | G437G (GGT→GGG) | fruA | fused fructose‑specific PTS enzyme IIB'BC components |
* | AP012306 | 2,714,555 | 0 | T | G | 22.9% | 749.5 / 19.7 | 303 | T175P (ACC→CCC) | ygjV | conserved inner membrane protein |
* | AP012306 | 3,645,642 | 0 | A | C | 22.4% | 888.5 / 25.5 | 336 | T318P (ACC→CCC) | aceB | malate synthase A |
* | AP012306 | 1,267,578 | 0 | T | G | 22.2% | 214.8 / 27.1 | 99 | D60A (GAC→GCC) | ddpB | D‑Ala‑D‑Ala transporter subunit |
* | AP012306 | 2,212,753 | 0 | A | C | 21.9% | 465.4 / 50.3 | 178 | Y241D (TAT→GAT) | hcaT | predicted 3‑phenylpropionic transporter |
* | AP012306 | 406,677 | 0 | A | G | 21.7% | 488.6 / 15.6 | 189 | K294E (AAA→GAA) | hemH | ferrochelatase |
* | AP012306 | 3,590,361 | 0 | G | A | 21.3% | 733.8 / 116.6 | 292 | E28K (GAA→AAA) | fabR | DNA‑binding transcriptional repressor |
* | AP012306 | 1,074,619 | 0 | T | C | 21.0% | 403.1 / 92.9 | 200 | S29G (AGC→GGC) | ldrA | toxic polypeptide, small |
* | AP012306 | 3,182,506 | 0 | A | C | 21.0% | 244.8 / 10.8 | 100 | D80A (GAC→GCC) | yiaN | predicted transporter |
Marginal new junction evidence (lowest skew 10 of 153 shown) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | AP012306 | = 997497 | 305 (0.930) | 11 (0.040) | 10/268 | 17.8 | 3.8% | coding (41/1305 nt) | ndh | respiratory NADH dehydrogenase 2/cupric reductase |
? | AP012306 | 997993 = | 281 (0.960) | coding (537/1305 nt) | ndh | respiratory NADH dehydrogenase 2/cupric reductase | |||||
* | ? | AP012306 | = 213442 | NA (NA) | 10 (0.030) | 9/284 | 18.7 | NA | noncoding (811/1542 nt) | rrsH | 16S ribosomal RNA (rrsH) |
? | AP012306 | = 213476 | NA (NA) | noncoding (845/1542 nt) | rrsH | 16S ribosomal RNA (rrsH) | |||||
* | ? | AP012306 | = 215364 | NA (NA) | 9 (0.030) | 9/288 | 18.8 | NA | noncoding (745/2905 nt) | rrlH | 23S ribosomal RNA (rrlH) |
? | AP012306 | = 215372 | NA (NA) | noncoding (753/2905 nt) | rrlH | 23S ribosomal RNA (rrlH) | |||||
* | ? | AP012306 | = 1559316 | 379 (1.160) | 8 (0.030) | 8/286 | 19.2 | 2.2% | coding (86/249 nt) | yeaQ | conserved inner membrane protein |
? | AP012306 | = 1559327 | 334 (1.070) | coding (75/249 nt) | yeaQ | conserved inner membrane protein | |||||
* | ? | AP012306 | 3044657 = | 336 (1.030) | 7 (0.020) | 7/290 | 19.8 | 2.1% | coding (921/1110 nt) | livK | leucine transporter subunit |
? | AP012306 | 3044673 = | 325 (1.020) | coding (905/1110 nt) | livK | leucine transporter subunit | |||||
* | ? | AP012306 | 3910038 = | 411 (1.260) | 5 (0.020) | 5/250 | 19.9 | 1.4% | intergenic (+10/+46) | yjgN/valS | conserved inner membrane protein/valyl‑tRNA synthetase |
? | AP012306 | 3910062 = | 351 (1.280) | intergenic (+34/+22) | yjgN/valS | conserved inner membrane protein/valyl‑tRNA synthetase | |||||
* | ? | AP012306 | = 891201 | 316 (0.970) | 6 (0.020) | 6/274 | 20.0 | 2.0% | coding (254/318 nt) | yccV | DNA‑binding protein, hemimethylated |
? | AP012306 | = 891189 | 293 (0.980) | coding (266/318 nt) | yccV | DNA‑binding protein, hemimethylated | |||||
* | ? | AP012306 | = 2498245 | 490 (1.500) | 6 (0.020) | 6/284 | 20.2 | 1.3% | coding (175/633 nt) | yqeH | conserved protein with bipartite regulator domain |
? | AP012306 | = 2498272 | 450 (1.450) | coding (202/633 nt) | yqeH | conserved protein with bipartite regulator domain | |||||
* | ? | AP012306 | = 2243307 | 262 (0.800) | 6 (0.020) | 6/282 | 20.2 | 2.3% | coding (211/537 nt) | tadA | tRNA‑specific adenosine deaminase |
? | AP012306 | = 2243325 | 252 (0.820) | coding (193/537 nt) | tadA | tRNA‑specific adenosine deaminase | |||||
* | ? | AP012306 | 2250214 = | 372 (1.140) | 6 (0.020) | 6/288 | 20.4 | 1.6% | coding (723/975 nt) | lepB | leader peptidase |
? | AP012306 | 2250249 = | 370 (1.170) | coding (688/975 nt) | lepB | leader peptidase |