breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 25 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG38.3% 449.9 / 62.1 269intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
*AP012306644,4170GA33.1% 711.1 / inf 525intergenic (+32/‑401)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
*AP0123063,182,8610AC31.0% 336.5 / 39.3 155L198F (TTA→TTCyiaNpredicted transporter
*AP0123061,226,5570AG30.3% 374.1 / 125.1 211G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*AP0123061,944,2880AC30.0% 559.0 / 37.5 250G361G (GGT→GGGmenEo‑succinylbenzoate‑CoA ligase
*AP0123063,938,8870CT27.7% 492.4 / 137.1 242S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
*AP0123063,527,3680AC27.4% 639.5 / 59.1 270L140F (TTA→TTCrhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
*AP012306800,6750AC26.6% 668.3 / 27.4 290T192P (ACC→CCC) lolAchaperone for lipoproteins
*AP012306341,3770TG26.3% 256.0 / 15.6 137G60G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*AP0123063,590,3670AC25.0% 745.4 / 36.1 284T30P (ACC→CCC) fabRDNA‑binding transcriptional repressor
*AP0123061,854,8510AC24.6% 711.6 / 68.1 284T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
*AP0123061,834,2470AC23.5% 457.5 / 13.6 183G437G (GGT→GGGfruAfused fructose‑specific PTS enzyme IIB'BC components
*AP0123062,714,5550TG22.9% 749.5 / 19.7 303T175P (ACC→CCC) ygjVconserved inner membrane protein
*AP0123063,645,6420AC22.4% 888.5 / 25.5 336T318P (ACC→CCC) aceBmalate synthase A
*AP0123061,267,5780TG22.2% 214.8 / 27.1 99D60A (GAC→GCC) ddpBD‑Ala‑D‑Ala transporter subunit
*AP0123062,212,7530AC21.9% 465.4 / 50.3 178Y241D (TAT→GAT) hcaTpredicted 3‑phenylpropionic transporter
*AP012306406,6770AG21.7% 488.6 / 15.6 189K294E (AAA→GAA) hemHferrochelatase
*AP0123063,590,3610GA21.3% 733.8 / 116.6 292E28K (GAA→AAA) fabRDNA‑binding transcriptional repressor
*AP0123061,074,6190TC21.0% 403.1 / 92.9 200S29G (AGC→GGC) ldrAtoxic polypeptide, small
*AP0123063,182,5060AC21.0% 244.8 / 10.8 100D80A (GAC→GCC) yiaNpredicted transporter

Marginal new junction evidence (lowest skew 10 of 153 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 = 997497305 (0.930)11 (0.040) 10/268 17.8 3.8% coding (41/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
?AP012306 997993 = 281 (0.960)coding (537/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
* ? AP012306 = 213442NA (NA)10 (0.030) 9/284 18.7 NA noncoding (811/1542 nt) rrsH 16S ribosomal RNA (rrsH)
?AP012306 = 213476 NA (NA)noncoding (845/1542 nt) rrsH 16S ribosomal RNA (rrsH)
* ? AP012306 = 215364NA (NA)9 (0.030) 9/288 18.8 NA noncoding (745/2905 nt) rrlH 23S ribosomal RNA (rrlH)
?AP012306 = 215372 NA (NA)noncoding (753/2905 nt) rrlH 23S ribosomal RNA (rrlH)
* ? AP012306 = 1559316379 (1.160)8 (0.030) 8/286 19.2 2.2% coding (86/249 nt) yeaQ conserved inner membrane protein
?AP012306 = 1559327 334 (1.070)coding (75/249 nt) yeaQ conserved inner membrane protein
* ? AP012306 3044657 =336 (1.030)7 (0.020) 7/290 19.8 2.1% coding (921/1110 nt) livK leucine transporter subunit
?AP012306 3044673 = 325 (1.020)coding (905/1110 nt) livK leucine transporter subunit
* ? AP012306 3910038 =411 (1.260)5 (0.020) 5/250 19.9 1.4% intergenic (+10/+46) yjgN/valS conserved inner membrane protein/valyl‑tRNA synthetase
?AP012306 3910062 = 351 (1.280)intergenic (+34/+22) yjgN/valS conserved inner membrane protein/valyl‑tRNA synthetase
* ? AP012306 = 891201316 (0.970)6 (0.020) 6/274 20.0 2.0% coding (254/318 nt) yccV DNA‑binding protein, hemimethylated
?AP012306 = 891189 293 (0.980)coding (266/318 nt) yccV DNA‑binding protein, hemimethylated
* ? AP012306 = 2498245490 (1.500)6 (0.020) 6/284 20.2 1.3% coding (175/633 nt) yqeH conserved protein with bipartite regulator domain
?AP012306 = 2498272 450 (1.450)coding (202/633 nt) yqeH conserved protein with bipartite regulator domain
* ? AP012306 = 2243307262 (0.800)6 (0.020) 6/282 20.2 2.3% coding (211/537 nt) tadA tRNA‑specific adenosine deaminase
?AP012306 = 2243325 252 (0.820)coding (193/537 nt) tadA tRNA‑specific adenosine deaminase
* ? AP012306 2250214 =372 (1.140)6 (0.020) 6/288 20.4 1.6% coding (723/975 nt) lepB leader peptidase
?AP012306 2250249 = 370 (1.170)coding (688/975 nt) lepB leader peptidase