breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 31 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG48.2% 305.2 / 110.5 255intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
*AP0123061,944,2880AC38.8% 348.7 / 79.1 227G361G (GGT→GGGmenEo‑succinylbenzoate‑CoA ligase
*AP0123063,527,3680AC31.5% 635.8 / 105.9 308L140F (TTA→TTCrhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
*AP0123063,020,6700TG31.3% 334.5 / 21.8 198K195N (AAA→AACglgCglucose‑1‑phosphate adenylyltransferase
*AP012306800,6750AC30.7% 595.7 / 55.2 287T192P (ACC→CCC) lolAchaperone for lipoproteins
*AP012306644,4170GA29.2% 1006.9 / inf 606intergenic (+32/‑401)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
*AP0123063,938,8870CT28.1% 612.6 / 184.8 302S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
*AP012306202,4170TG27.9% 603.4 / 55.5 290V409G (GTA→GGA) tilStRNA(Ile)‑lysidine synthetase
*AP0123061,309,9140AC27.9% 267.0 / 14.9 140L23F (TTA→TTClsrCAI2 transporter
*AP0123062,714,5550TG26.3% 713.4 / 33.8 328T175P (ACC→CCC) ygjVconserved inner membrane protein
*AP012306935,0540GT26.0% 211.4 / 28.3 77intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
*AP0123061,226,5570AG25.9% 440.8 / 107.9 212G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*AP0123062,558,0710AC25.8% 727.1 / 44.4 306V302G (GTA→GGA) visCpredicted oxidoreductase, FAD/NAD(P)‑binding domain
*AP0123063,188,0800TG25.8% 426.0 / 20.5 183G177G (GGT→GGGsgbEL‑ribulose‑5‑phosphate 4‑epimerase
*AP0123062,680,3450AC25.7% 416.0 / 40.1 202T184P (ACC→CCC) ttdAL‑tartrate dehydratase, alpha subunit
*AP0123062,758,9090TG25.6% 671.4 / 21.3 274G33G (GGT→GGGagaDN‑acetylgalactosamine‑specific enzyme IID component of PTS
*AP0123063,282,0760TG25.4% 605.9 / 18.2 272intergenic (‑79/+27)ilvB/ivbLacetolactate synthase I, large subunit/ilvB operon leader peptide
*AP012306341,3770TG25.2% 248.7 / 11.9 119G60G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*AP0123063,639,0400TG24.4% 165.0 / 15.3 78noncoding (54/76 nt)gltVtRNA‑Glu
*AP0123061,854,8510AC23.8% 750.5 / 60.4 298T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase

Marginal new junction evidence (lowest skew 10 of 624 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 = 3600650NA (NA)58 (0.170)
+AAAGG
17/288 6.6 NA noncoding (2797/2904 nt) rrlB 23S ribosomal RNA (rrlB)
?AP012306 = 3642048 NA (NA)noncoding (2793/2904 nt) rrlE 23S ribosomal RNA (rrlE)
* ? AP012306 = 215364NA (NA)20 (0.060) 17/288 6.6 NA noncoding (745/2905 nt) rrlH 23S ribosomal RNA (rrlH)
?AP012306 = 215372 NA (NA)noncoding (753/2905 nt) rrlH 23S ribosomal RNA (rrlH)
* ? AP012306 = 3600658NA (NA)40 (0.110) 17/292 6.7 NA noncoding (2805/2904 nt) rrlB 23S ribosomal RNA (rrlB)
?AP012306 = 3642045 NA (NA)noncoding (2790/2904 nt) rrlE 23S ribosomal RNA (rrlE)
* ? AP012306 = 1092512385 (1.070)17 (0.050) 12/258 7.1 5.0% intergenic (+100/+3) ychS/tpr predicted protein/predicted protamine‑like protein
?AP012306 1092985 = 314 (1.010)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 1092334280 (0.780)12 (0.040) 10/258 7.5 4.1% coding (198/276 nt) ychS predicted protein
?AP012306 1092985 = 314 (1.010)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 913279341 (0.950)11 (0.030) 11/274 7.5 3.4% coding (979/1173 nt) torC trimethylamine N‑oxide (TMAO) reductase I, cytochrome c‑type subunit
?AP012306 = 913267 308 (0.930)coding (967/1173 nt) torC trimethylamine N‑oxide (TMAO) reductase I, cytochrome c‑type subunit
* ? AP012306 3561599 =382 (1.060)11 (0.030) 11/290 7.7 2.8% intergenic (+119/‑230) metL/metF fused aspartokinase II/homoserine dehydrogenase II/5,10‑methylenetetrahydrofolate reductase
?AP012306 3561629 = 391 (1.120)intergenic (+149/‑200) metL/metF fused aspartokinase II/homoserine dehydrogenase II/5,10‑methylenetetrahydrofolate reductase
* ? AP012306 = 2659620317 (0.880)10 (0.030) 10/274 7.7 3.3% coding (1257/1482 nt) tolC transport channel
?AP012306 = 2659608 290 (0.880)coding (1245/1482 nt) tolC transport channel
* ? AP012306 = 2049432502 (1.400)10 (0.030) 10/274 7.7 2.1% coding (470/1146 nt) yfdE predicted CoA‑transferase, NAD(P)‑binding
?AP012306 = 2049420 464 (1.410)coding (482/1146 nt) yfdE predicted CoA‑transferase, NAD(P)‑binding
* ? AP012306 = 1883833518 (1.440)10 (0.030) 10/274 7.7 2.0% intergenic (‑717/‑22) ompC/rcsD outer membrane porin protein C/phosphotransfer intermediate protein in two‑component regulatory system with RcsBC
?AP012306 = 1883821 488 (1.480)intergenic (‑705/‑34) ompC/rcsD outer membrane porin protein C/phosphotransfer intermediate protein in two‑component regulatory system with RcsBC