breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 25 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG36.4% 475.6 / 54.2 243intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
*AP0123061,944,2880AC35.6% 395.0 / 63.3 219G361G (GGT→GGGmenEo‑succinylbenzoate‑CoA ligase
*AP0123063,020,6700TG33.3% 383.0 / 11.5 219K195N (AAA→AACglgCglucose‑1‑phosphate adenylyltransferase
*AP012306644,4170GA31.9% 809.1 / inf 561intergenic (+32/‑401)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
*AP0123061,226,5570AG28.0% 366.1 / 82.6 175G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*AP0123062,212,7530AC27.7% 374.5 / 53.8 159Y241D (TAT→GAT) hcaTpredicted 3‑phenylpropionic transporter
*AP012306800,6750AC27.5% 542.7 / 45.4 241T192P (ACC→CCC) lolAchaperone for lipoproteins
*AP012306341,3770TG27.4% 256.2 / 19.8 146G60G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*AP0123063,938,8870CT27.3% 516.5 / 145.1 249S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
*AP0123063,527,3680AC27.0% 689.0 / 43.6 272L140F (TTA→TTCrhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
*AP0123062,758,9090TG26.7% 515.7 / 13.3 206G33G (GGT→GGGagaDN‑acetylgalactosamine‑specific enzyme IID component of PTS
*AP0123061,854,8510AC26.0% 735.0 / 72.5 296T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
*AP012306935,0540GT25.3% 260.1 / 39.0 95intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
*AP0123061,128,4120TG24.4% 822.9 / 48.8 320V29G (GTG→GGG) yciOconserved protein
*AP0123062,714,5550TG23.7% 709.7 / 17.3 291T175P (ACC→CCC) ygjVconserved inner membrane protein
*AP012306202,4170TG23.3% 657.4 / 38.6 270V409G (GTA→GGA) tilStRNA(Ile)‑lysidine synthetase
*AP0123063,094,1840AC23.2% 1118.1 / 47.7 452V209G (GTG→GGG) yhiDpredicted Mg(2+) transport ATPase inner membrane protein
*AP0123063,590,3670AC22.2% 834.1 / 31.9 293T30P (ACC→CCC) fabRDNA‑binding transcriptional repressor
*AP0123062,499,4140AC21.9% 972.1 / 74.7 369P126P (CCA→CCCyqeIpredicted transcriptional regulator
*AP0123062,560,2430TG21.4% 526.7 / 19.0 206N419T (AAC→ACC) pepPproline aminopeptidase P II

Marginal new junction evidence (lowest skew 10 of 38 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 = 997497346 (1.060)15 (0.050) 15/268 12.0 4.7% coding (41/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
?AP012306 997993 = 299 (1.020)coding (537/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
* ? AP012306 = 1092512389 (1.200)14 (0.050) 11/258 13.0 4.3% intergenic (+100/+3) ychS/tpr predicted protein/predicted protamine‑like protein
?AP012306 1092985 = 280 (1.000)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 1092334285 (0.880)9 (0.030) 8/258 14.1 3.3% coding (198/276 nt) ychS predicted protein
?AP012306 1092985 = 280 (1.000)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 644310 =351 (1.080)29 (0.090)
+GAG
8/292 14.8 7.8% noncoding (1/76 nt) lysQ tRNA‑Lys
?AP012306 = 2073420 351 (1.080)noncoding (76/76 nt) valU tRNA‑Val
* ? AP012306 = 2275071215 (0.660)45 (0.160)
+GTATCAGTCTGCTTCGCAAG
6/258 15.0 15.5% noncoding (1/76 nt) gltW tRNA‑Glu
?AP012306 = 3597478 354 (1.090)intergenic (+65/‑107) rrsB/gltT 16S ribosomal RNA (rrnB)/tRNA‑Glu
* ? AP012306 644310 =351 (1.080)26 (0.080)
+G
6/296 15.8 7.2% noncoding (1/76 nt) lysQ tRNA‑Lys
?AP012306 = 2073542 323 (0.990)intergenic (+2/‑45) valX/valY tRNA‑Val/tRNA‑Val
* ? AP012306 3165385 =423 (1.300)5 (0.020) 5/274 15.9 1.3% coding (1451/1455 nt) xylB xylulokinase
?AP012306 3165397 = 396 (1.320)coding (1439/1455 nt) xylB xylulokinase
* ? AP012306 = 1810933239 (0.730)5 (0.020) 5/288 16.1 2.2% coding (968/1011 nt) mglC methyl‑galactoside transporter subunit
?AP012306 = 1810942 222 (0.710)coding (959/1011 nt) mglC methyl‑galactoside transporter subunit
* ? AP012306 805307 =309 (0.950)5 (0.020) 5/292 16.2 1.6% coding (1616/2445 nt) dmsA dimethyl sulfoxide reductase, anaerobic, subunit A
?AP012306 805325 = 297 (0.930)coding (1634/2445 nt) dmsA dimethyl sulfoxide reductase, anaerobic, subunit A
* ? AP012306 = 795718396 (1.220)5 (0.020) 5/294 16.3 1.3% coding (391/495 nt) lrp DNA‑binding transcriptional dual regulator, leucine‑binding
?AP012306 = 795731 390 (1.220)coding (404/495 nt) lrp DNA‑binding transcriptional dual regulator, leucine‑binding