breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6061,004,1060AC45.3% 75.5 / 20.8 53intergenic (‑32/+570)ompF/asnSouter membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase
*REL6063,983,7000TG44.4% 32.2 / 20.9 36T397P (ACC→CCC) ECB_03713conserved hypothetical protein
*REL6061,688,9900AC42.5% 61.5 / 22.0 47T92P (ACC→CCC) nthDNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)
*REL6061,484,1050AG37.1% 42.6 / 19.3 35G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*REL6063,492,0820TG33.3% 70.9 / 16.4 42V490G (GTG→GGG) glpDsn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
*REL606500,2130AC31.6% 62.3 / 10.5 38H33P (CAC→CCC) ylbHconserved protein, rhs‑like
*REL6064,141,0090GA29.8% 99.8 / 15.5 47E28K (GAA→AAA) yijCDNA‑binding transcriptional repressor
*REL6061,747,9030GT29.2% 149.7 / 50.7 65A8S (GCA→TCA) ydiLhypothetical protein
*REL6064,195,8740AC29.2% 104.5 / 11.4 48T318P (ACC→CCC) aceBmalate synthase
*REL606154,4970TG28.8% 114.5 / 10.9 52A191A (GCA→GCCyadLpredicted fimbrial‑like adhesin protein
*REL6061,875,4830GC20.0% 221.3 / 41.1 75intergenic (+156/‑28)yeaB/sdaApredicted NUDIX hydrolase/L‑serine deaminase I

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 2574614 =75 (1.380)4 (0.090) 4/126 5.3 6.3% intergenic (+456/+362) sseA/sseB 3‑mercaptopyruvate sulfurtransferase/rhodanase‑like enzyme, sulfur transfer from thiosulfate
?REL606 2574646 = 57 (1.260)intergenic (+488/+330) sseA/sseB 3‑mercaptopyruvate sulfurtransferase/rhodanase‑like enzyme, sulfur transfer from thiosulfate
* ? REL606 = 271869673 (1.350)6 (0.130) 4/130 5.4 8.6% intergenic (‑68/+12) recA/ygaD recombinase A/competence damage‑inducible protein A
?REL606 = 2718698 64 (1.370)intergenic (‑70/+10) recA/ygaD recombinase A/competence damage‑inducible protein A
* ? REL606 = 267962388 (1.620)7 (0.140) 4/140 5.8 8.2% coding (695/1557 nt) ECB_02510 Recombinase
?REL606 = 2680101 74 (1.460)coding (1173/1557 nt) ECB_02510 Recombinase
* ? REL606 = 54095278 (1.440)7 (0.150) 3/132 6.1 8.6% coding (161/1527 nt) ybcK predicted recombinase
?REL606 = 541418 80 (1.680)coding (627/1527 nt) ybcK predicted recombinase
* ? REL606 3026311 =83 (1.530)8 (0.160) 3/140 6.3 9.3% coding (492/1377 nt) ECB_02826 hypothetical protein
?REL606 3026805 = 79 (1.560)intergenic (‑3/+206) ECB_02826/ECB_02827 hypothetical protein/Polysialic acid transport ATP‑binding KpsT