breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | REL606 | 3,492,082 | 0 | T | G | 43.9% | 81.9 / 36.3 | 66 | V490G (GTG→GGG) | glpD | sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
* | REL606 | 4,393,061 | 0 | T | G | 43.8% | 42.9 / 12.8 | 32 | V211G (GTG→GGG) | aidB | isovaleryl CoA dehydrogenase |
* | REL606 | 1,004,106 | 0 | A | C | 39.4% | 114.3 / 24.4 | 66 | intergenic (‑32/+570) | ompF/asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase |
* | REL606 | 2,296,329 | 0 | A | C | 35.5% | 115.9 / 27.1 | 62 | intergenic (‑149/‑66) | inaA/yfaH | hypothetical protein/hypothetical protein |
* | REL606 | 1,875,483 | 0 | G | C | 35.1% | 169.5 / 128.6 | 112 | intergenic (+156/‑28) | yeaB/sdaA | predicted NUDIX hydrolase/L‑serine deaminase I |
* | REL606 | 2,174,648 | 0 | T | G | 34.0% | 75.5 / 26.7 | 50 | V409G (GTG→GGG) | dld | D‑lactate dehydrogenase, FAD‑binding, NADH independent |
* | REL606 | 2,631,772 | 0 | A | C | 33.9% | 115.8 / 21.0 | 59 | intergenic (‑267/‑141) | rpoE/nadB | RNA polymerase, sigma 24 (sigma E) factor/L‑aspartate oxidase |
* | REL606 | 254,101 | 0 | T | G | 33.3% | 40.4 / 10.5 | 31 | pseudogene (530/1710 nt) | fhiA | b0229; flagellar system protein |
* | REL606 | 3,691,844 | 0 | T | G | 33.3% | 78.3 / 13.7 | 45 | N226T (AAC→ACC) | yiaY | predicted Fe‑containing alcohol dehydrogenase |
* | REL606 | 1,484,105 | 0 | A | G | 30.9% | 71.6 / 23.8 | 43 | G55G (GGA→GGG) | ydcU | predicted spermidine/putrescine transporter subunit |
* | REL606 | 1,747,903 | 0 | G | T | 27.5% | 263.2 / 76.0 | 102 | A8S (GCA→TCA) | ydiL | hypothetical protein |
* | REL606 | 1,795,650 | 0 | A | C | 27.0% | 154.2 / 12.2 | 63 | V39G (GTG→GGG) | celF | cryptic phospho‑beta‑glucosidase, NAD(P)‑binding |
* | REL606 | 1,176,010 | 0 | T | G | 26.2% | 144.0 / 14.3 | 61 | A37A (GCA→GCC) | fhuE | ferric‑rhodotorulic acid outer membrane transporter |
* | REL606 | 4,169,232 | 0 | A | T | 25.0% | 125.2 / 17.4 | 56 | intergenic (+48/‑165) | rpoC/htrC | DNA‑directed RNA polymerase subunit beta'/heat shock protein |
* | REL606 | 1,416,838 | 0 | T | G | 23.4% | 264.6 / 19.0 | 107 | intergenic (+1/‑4) | ECB_01334/ECB_01335 | putative Lysozyme/conserved hypothetical protein |
* | REL606 | 1,688,990 | 0 | A | C | 22.2% | 140.6 / 11.7 | 54 | T92P (ACC→CCC) | nth | DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III) |
* | REL606 | 154,497 | 0 | T | G | 21.3% | 157.6 / 12.3 | 61 | A191A (GCA→GCC) | yadL | predicted fimbrial‑like adhesin protein |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | 4305592 = | 73 (1.120) | 5 (0.090) | 5/132 | 6.9 | 7.0% | intergenic (‑144/‑257) | phnA/yjdA | hypothetical protein/conserved protein with nucleoside triphosphate hydrolase domain |
? | REL606 | 4305616 = | 69 (1.210) | intergenic (‑168/‑233) | phnA/yjdA | hypothetical protein/conserved protein with nucleoside triphosphate hydrolase domain | |||||
* | ? | REL606 | = 2039252 | 33 (0.510) | 7 (0.120) | 5/140 | 7.2 | 15.3% | coding (443/747 nt) | wbbA | putative glycosyltransferase WbbA |
? | REL606 | = 2039725 | 47 (0.770) | coding (404/438 nt) | vioB | VioB, involved in dTDP‑N‑acetylviosamine synthesis | |||||
* | ? | REL606 | 2041054 = | 50 (0.770) | 6 (0.100) | 4/136 | 7.6 | 10.2% | coding (330/1113 nt) | vioA | VioA, involved in dTDP‑N‑acetylviosamine synthesis |
? | REL606 | 2041468 = | 61 (1.030) | coding (1353/1428 nt) | wzx | O antigen translocase (Wzx) | |||||
* | ? | REL606 | = 2038375 | 41 (0.630) | 3 (0.050) | 3/132 | 8.0 | 6.8% | coding (574/822 nt) | wbbB | putative glycosyltransferase WbbB |
? | REL606 | = 2038763 | 46 (0.800) | coding (186/822 nt) | wbbB | putative glycosyltransferase WbbB | |||||
* | ? | REL606 | 2038725 = | 52 (0.800) | 3 (0.050) | 3/138 | 8.3 | 7.2% | coding (224/822 nt) | wbbB | putative glycosyltransferase WbbB |
? | REL606 | 2039200 = | 30 (0.500) | coding (495/747 nt) | wbbA | putative glycosyltransferase WbbA | |||||
* | ? | REL606 | 2041220 = | 48 (0.740) | 4 (0.060) | 3/142 | 8.4 | 6.9% | coding (164/1113 nt) | vioA | VioA, involved in dTDP‑N‑acetylviosamine synthesis |
? | REL606 | 2041465 = | 63 (1.020) | coding (1356/1428 nt) | wzx | O antigen translocase (Wzx) | |||||
* | ? | REL606 | = 2038070 | 72 (1.110) | 4 (0.070) | 3/140 | 8.4 | 6.5% | coding (61/1176 nt) | wzy | O antigen polymerase (Wzy) |
? | REL606 | = 2038459 | 48 (0.790) | coding (490/822 nt) | wbbB | putative glycosyltransferase WbbB |