breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6063,492,0820TG43.9% 81.9 / 36.3 66V490G (GTG→GGG) glpDsn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
*REL6064,393,0610TG43.8% 42.9 / 12.8 32V211G (GTG→GGG) aidBisovaleryl CoA dehydrogenase
*REL6061,004,1060AC39.4% 114.3 / 24.4 66intergenic (‑32/+570)ompF/asnSouter membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase
*REL6062,296,3290AC35.5% 115.9 / 27.1 62intergenic (‑149/‑66)inaA/yfaHhypothetical protein/hypothetical protein
*REL6061,875,4830GC35.1% 169.5 / 128.6 112intergenic (+156/‑28)yeaB/sdaApredicted NUDIX hydrolase/L‑serine deaminase I
*REL6062,174,6480TG34.0% 75.5 / 26.7 50V409G (GTG→GGG) dldD‑lactate dehydrogenase, FAD‑binding, NADH independent
*REL6062,631,7720AC33.9% 115.8 / 21.0 59intergenic (‑267/‑141)rpoE/nadBRNA polymerase, sigma 24 (sigma E) factor/L‑aspartate oxidase
*REL606254,1010TG33.3% 40.4 / 10.5 31pseudogene (530/1710 nt)fhiAb0229; flagellar system protein
*REL6063,691,8440TG33.3% 78.3 / 13.7 45N226T (AAC→ACC) yiaYpredicted Fe‑containing alcohol dehydrogenase
*REL6061,484,1050AG30.9% 71.6 / 23.8 43G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*REL6061,747,9030GT27.5% 263.2 / 76.0 102A8S (GCA→TCA) ydiLhypothetical protein
*REL6061,795,6500AC27.0% 154.2 / 12.2 63V39G (GTG→GGG) celFcryptic phospho‑beta‑glucosidase, NAD(P)‑binding
*REL6061,176,0100TG26.2% 144.0 / 14.3 61A37A (GCA→GCCfhuEferric‑rhodotorulic acid outer membrane transporter
*REL6064,169,2320AT25.0% 125.2 / 17.4 56intergenic (+48/‑165)rpoC/htrCDNA‑directed RNA polymerase subunit beta'/heat shock protein
*REL6061,416,8380TG23.4% 264.6 / 19.0 107intergenic (+1/‑4)ECB_01334/ECB_01335putative Lysozyme/conserved hypothetical protein
*REL6061,688,9900AC22.2% 140.6 / 11.7 54T92P (ACC→CCC) nthDNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)
*REL606154,4970TG21.3% 157.6 / 12.3 61A191A (GCA→GCCyadLpredicted fimbrial‑like adhesin protein

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 4305592 =73 (1.120)5 (0.090) 5/132 6.9 7.0% intergenic (‑144/‑257) phnA/yjdA hypothetical protein/conserved protein with nucleoside triphosphate hydrolase domain
?REL606 4305616 = 69 (1.210)intergenic (‑168/‑233) phnA/yjdA hypothetical protein/conserved protein with nucleoside triphosphate hydrolase domain
* ? REL606 = 203925233 (0.510)7 (0.120) 5/140 7.2 15.3% coding (443/747 nt) wbbA putative glycosyltransferase WbbA
?REL606 = 2039725 47 (0.770)coding (404/438 nt) vioB VioB, involved in dTDP‑N‑acetylviosamine synthesis
* ? REL606 2041054 =50 (0.770)6 (0.100) 4/136 7.6 10.2% coding (330/1113 nt) vioA VioA, involved in dTDP‑N‑acetylviosamine synthesis
?REL606 2041468 = 61 (1.030)coding (1353/1428 nt) wzx O antigen translocase (Wzx)
* ? REL606 = 203837541 (0.630)3 (0.050) 3/132 8.0 6.8% coding (574/822 nt) wbbB putative glycosyltransferase WbbB
?REL606 = 2038763 46 (0.800)coding (186/822 nt) wbbB putative glycosyltransferase WbbB
* ? REL606 2038725 =52 (0.800)3 (0.050) 3/138 8.3 7.2% coding (224/822 nt) wbbB putative glycosyltransferase WbbB
?REL606 2039200 = 30 (0.500)coding (495/747 nt) wbbA putative glycosyltransferase WbbA
* ? REL606 2041220 =48 (0.740)4 (0.060) 3/142 8.4 6.9% coding (164/1113 nt) vioA VioA, involved in dTDP‑N‑acetylviosamine synthesis
?REL606 2041465 = 63 (1.020)coding (1356/1428 nt) wzx O antigen translocase (Wzx)
* ? REL606 = 203807072 (1.110)4 (0.070) 3/140 8.4 6.5% coding (61/1176 nt) wzy O antigen polymerase (Wzy)
?REL606 = 2038459 48 (0.790)coding (490/822 nt) wbbB putative glycosyltransferase WbbB