breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 29 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,993,8140GA52.8% ‑0.1 / 47.9 36intergenic (+103/‑443)kefB‑III/PP_mr64glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
*NC_0029475,993,9160AC52.8% 3.8 / 44.2 36intergenic (+205/‑341)kefB‑III/PP_mr64glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
*NC_0029471,332,0760CT52.6% 8.3 / 52.3 38intergenic (+97/‑196)PP_16SE/PP_23SE16S ribosomal RNA/23S ribosomal RNA
*NC_0029474,353,6980AG47.5% 15.7 / 55.2 40intergenic (‑70/+404)PP_3820/galUgroup II intron‑encoding maturase/UTP‑glucose‑1‑phosphate uridylyltransferase
*NC_0029473,452,2780AC46.4% 8.9 / 22.2 61S18R (AGC→CGC) PP_5556bacteriophage lysis protein
*NC_002947699,7250AG44.4% 5.3 / 20.6 18intergenic (+115/‑129)PP_t06/PP_23SDtRNA‑Ala/23S ribosomal RNA
*NC_0029472,092,4430GA43.8% 20.1 / 30.8 32intergenic (+264/+654)PP_1865/aroHtransposase/3‑deoxy‑7‑phosphoheptulonate synthase
*NC_0029472,092,4450TG43.8% 9.4 / 42.1 32intergenic (+266/+652)PP_1865/aroHtransposase/3‑deoxy‑7‑phosphoheptulonate synthase
*NC_0029472,092,4470AC43.8% 8.5 / 36.9 32intergenic (+268/+650)PP_1865/aroHtransposase/3‑deoxy‑7‑phosphoheptulonate synthase
*NC_0029473,638,0410TG40.0% 71.1 / 19.0 70V174G (GTG→GGG) PP_3201BNR domain‑containing protein
*NC_0029471,752,3420TG38.0% 30.1 / 17.6 51E53A (GAG→GCG) PP_5473LuxR family transcriptional regulator
*NC_002947544,1060GA37.5% 9.2 / 12.5 16K781K (AAG→AAArpoCDNA‑directed RNA polymerase subunit beta'
*NC_0029473,452,2980AC35.7% 62.5 / 12.6 56A24A (GCA→GCCPP_5556bacteriophage lysis protein
*NC_0029474,011,2920TG35.7% 64.9 / 10.3 48Q526P (CAG→CCG) PP_3535gamma‑glutamyltransferase
*NC_0029475,993,8080G.31.8% 149.2 / 73.3 77intergenic (+97/‑449)kefB‑III/PP_mr64glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
*NC_0029471,782,5120AC31.6% 100.5 / 13.8 57intergenic (+255/+37)PP_5476/PP_1585hypothetical protein/antidote protein
*NC_0029471,371,6030AG30.4% 66.7 / 18.3 46S18P (TCC→CCC) PP_1191S4 domain‑containing protein
*NC_0029471,622,7730CG27.3% 36.8 / 15.8 22intergenic (‑37/+136)PP_1417/PP_1418tricarboxylate transport protein TctB/tricarboxylate transport protein TctC
*NC_002947195,6340CT26.1% 35.1 / 11.2 24T380T (ACC→ACTPP_0168putative surface adhesion protein
*NC_0029476,075,1650AC26.0% 149.7 / 11.8 77V162G (GTG→GGG) PP_5324two‑component system response regulator

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_002947 2109155 =14 (0.580)3 (0.130) 3/252 1.8 16.5% coding (721/1515 nt) PP_1879 hypothetical protein
?NC_002947 5613493 = 17 (0.740)coding (1105/1272 nt) waaA 3‑deoxy‑D‑manno‑octulosonic acid transferase