breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 64 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,224,9860AC55.6% ‑5.3 / 20.5 37A427A (GCT→GCGPP_4603transposase
*NC_0029471,327,1270CT50.5% 306.1 / inf 684intergenic (+97/‑196)PP_16SE/PP_23SE16S ribosomal RNA/23S ribosomal RNA
*NC_0029475,224,9630AT50.0% ‑6.6 / 19.0 30L435Q (CTG→CAG) PP_4603transposase
*NC_0029474,350,6710AG49.6% 334.9 / inf 768intergenic (‑70/+404)PP_3820/galUgroup II intron‑encoding maturase/UTP‑glucose‑1‑phosphate uridylyltransferase
*NC_0029475,224,9690CG48.5% ‑6.0 / 12.4 34R433P (CGG→CCG) PP_4603transposase
*NC_002947699,7250AG46.0% 199.0 / inf 478intergenic (+115/‑129)PP_t06/PP_23SDtRNA‑Ala/23S ribosomal RNA
*NC_0029474,810,1470AC45.6% 24.5 / 76.8 57intergenic (‑289/‑266)PP_4234/dsbD‑IIbifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
*NC_0029474,810,1480AG45.6% 15.4 / 75.3 57intergenic (‑290/‑265)PP_4234/dsbD‑IIbifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
*NC_002947195,6340CT42.4% 411.1 / inf 687T380T (ACC→ACTPP_0168putative surface adhesion protein
*NC_0029475,224,9830CA33.3% ‑5.5 / 10.6 36L428F (TTG→TTTPP_4603transposase
*NC_002947195,5590CA28.8% 886.4 / inf 494T355T (ACC→ACAPP_0168putative surface adhesion protein
*NC_0029474,355,1750TG28.0% 352.6 / 42.5 207V112G (GTG→GGG) yrpB2‑nitropropane dioxygenase
*NC_0029471,544,4010CG27.3% 739.2 / 130.3 388A388G (GCG→GGG) mdfAmultidrug MFS transporter
*NC_002947196,3510CG26.1% 931.2 / inf 477T619T (ACC→ACGPP_0168putative surface adhesion protein
*NC_0029475,742,4490AC25.9% 331.4 / 16.5 186intergenic (+36/+35)hutF/PP_5037formiminoglutamate deiminase/lipocalin family lipoprotein
*NC_0029475,528,5130TA25.9% 977.8 / 99.8 484L19Q (CTG→CAG) PP_4860mannose‑6‑phosphate isomerase
*NC_002947196,8790GA24.9% 316.0 / 116.5 182V795V (GTG→GTAPP_0168putative surface adhesion protein
*NC_0029474,879,2910GC24.7% 726.9 / 84.9 312intergenic (‑372/‑28)pucM/puuE5‑hydroxyisourate hydrolase/allantoinase
*NC_0029474,160,4320TG24.6% 601.3 / 48.9 367L159R (CTG→CGG) PP_3661membrane protein
*NC_0029474,355,1700GC24.4% 442.1 / 40.2 217T110T (ACG→ACCyrpB2‑nitropropane dioxygenase

Marginal new junction evidence (lowest skew 10 of 549 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_002947 4810141 =53 (0.110)186 (0.500) 62/226 8.5 62.1% intergenic (‑283/‑272) PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
?NC_002947 = 5990913 187 (0.510)intergenic (+229/‑317) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
* ? NC_002947 = 1780108197 (0.410)69 (0.150) 57/282 12.7 28.8% intergenic (+25/+81) PP_1587/dapC NhaP‑type Na+(K+)/H+ antiporter/N‑succinyl‑L,L‑diaminopimelate aminotransferase
?NC_002947 1780171 = 154 (0.330)intergenic (+88/+18) PP_1587/dapC NhaP‑type Na+(K+)/H+ antiporter/N‑succinyl‑L,L‑diaminopimelate aminotransferase
* ? NC_002947 = 5203537279 (0.580)89 (0.190) 53/290 14.1 30.0% intergenic (‑53/‑127) PP_4582/PP_4583 membrane protein/putative Peptidase
?NC_002947 5203636 = 141 (0.300)intergenic (‑152/‑28) PP_4582/PP_4583 membrane protein/putative Peptidase
* ? NC_002947 = 5990749362 (0.750)59 (0.130) 45/280 15.5 16.0% intergenic (+65/‑481) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
?NC_002947 5990941 = 278 (0.610)intergenic (+257/‑289) kefB‑III/PP_mr64 glutathione‑regulated potassium/H+ antiporter/C4 ncRNA
* ? NC_002947 = 481014652 (0.110)133 (0.300)
+CGGCGCTTCGCGG
37/270 17.1 51.5% intergenic (‑288/‑267) PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
?NC_002947 4947183 = 223 (0.460)intergenic (+576/+222) PP_5673/flhB D‑alanine‑‑D‑alanine ligase/flagellin export apparatus substrate specificity protein
* ? NC_002947 1847008 =485 (1.000)41 (0.130)
+48 bp
11/200 23.6 11.2% coding (1257/1377 nt) pfeS‑II histidine kinase
?NC_002947 2437200 = NA (NA)intergenic (+82/‑222) PP_2133/PP_2134 hypothetical protein/transposase
* ? NC_002947 4587985 =263 (0.540)22 (0.060) 11/220 25.0 12.1% intergenic (+102/+115) PP_4061/PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
?NC_002947 4588089 = 125 (0.350)intergenic (+206/+11) PP_4061/PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
* ? NC_002947 = 4585460301 (0.620)25 (0.060) 14/256 25.5 9.5% intergenic (‑67/+104) treSB/glgE bifunctional trehalose synthase B/maltokinase/alpha‑1,4‑glucan:maltose‑1‑phosphate maltosyltransferase
?NC_002947 = 4585521 214 (0.510)intergenic (‑128/+43) treSB/glgE bifunctional trehalose synthase B/maltokinase/alpha‑1,4‑glucan:maltose‑1‑phosphate maltosyltransferase
* ? NC_002947 4539515 =NA (NA)60 (0.130) 16/286 26.1 46.3% coding (884/1533 nt) PP_4025 transposase
?NC_002947 = 4628624 72 (0.150)coding (848/1533 nt) PP_4091 transposase
* ? NC_002947 4539479 =NA (NA)61 (0.130) 16/286 26.1 NA coding (848/1533 nt) PP_4025 transposase
?NC_002947 4539515 = NA (NA)coding (884/1533 nt) PP_4025 transposase