breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 547,694 A→G intergenic (+123/‑1156) fdrA → / → ylbF acyl‑CoA synthetase with NAD(P)‑bindingRossmann‑fold domain/hypothetical protein
RA 547,835 +G intergenic (+264/‑1015) fdrA → / → ylbF acyl‑CoA synthetase with NAD(P)‑bindingRossmann‑fold domain/hypothetical protein
RA 556,858 A→T L36Q (CTG→CAG)  folD ← bifunctional 5,10‑methylene‑tetrahydrofolatedehydrogenase/5,10‑methylene ‑tetrahydrofolatecyclohydrolase
MC JC 654,214 Δ1,199 bp insH_12 insH_12
RA 967,490 G→T G150C (GGT→TGT)  msbA → lipid ABC transporter ATP‑bindingprotein/membrane protein
RA 987,574 G→T intergenic (‑170/+433) ompF ← / ← asnS outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
RA 1,093,686 T→C V130A (GTA→GCA)  ycdT → diguanylate cyclase
RA 1,197,797 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase,‑specific for NADP+
RA 1,197,809 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase,‑specific for NADP+
RA 1,197,822 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase,‑specific for NADP+
RA 1,197,824 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase,‑specific for NADP+
MC JC 1,301,041 Δ1,336 bp insC_8insD_10 insC_8, insD_10
RA 1,519,222 G→T V40V (GTG→GTT ydcX → inner membrane protein
RA 1,669,601 Δ1 bp coding (678/1221 nt) dgsA ← DNA‑binding transcriptional repressor
RA 1,979,957 G→T intergenic (‑37/+398) flhD ← / ← insH_10 DNA‑binding transcriptional dual regulator/IS5 element protein
RA 2,005,401 C→T E115K (GAA→AAA)  fliC ← flagellar filament structural protein
MC JC 2,176,983 Δ1,199 bp insH_11 insH_11
RA 2,245,455 A→G S283P (TCG→CCG)  yeiB ← inner membrane protein
MC JC 2,320,795 Δ1,336 bp insD_9insC_5 insD_9, insC_5
RA 2,866,111 A→G intergenic (‑21/+159) rpoS ← / ← nlpD RNA polymerase, sigma S (sigma 38) factor/outer membrane lipoprotein
RA 3,212,521 G→A M273I (ATG→ATA rpoD → RNA polymerase, sigma 70 (sigma D) factor
MC JC 3,261,134 Δ1,199 bp insH_16[tdcD] insH_16, [tdcD]
RA 3,268,825 A→G T186A (ACA→GCA)  yhaC → hypothetical protein
RA 3,693,457 +A noncoding (1417/1542 nt) rrsC ← 16S ribosomal RNA
MC JC 3,742,936 Δ1,336 bp insD_8insC insD_8, insC
MC JC 3,746,911 Δ1,199 bp insH_13 insH_13
MC JC 3,958,123 Δ34 bp coding (1156‑1189/1287 nt) dctA → C4‑dicarboxylic acid, orotate and citratetransporter
MC JC 4,310,363 Δ1,199 bp insH_15[alsK] insH_15, [alsK]
RA 4,371,274 (A)5→3 intergenic (‑9/+296) dcuA ← / ← aspA C4‑dicarboxylate antiporter/aspartate ammonia‑lyase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_007779_cfb 1197849 1213073 15225 56 [51] [50] 53 [icd]–mcrA [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,cohE,croE,ymfL,ymfM,ymfN,ymfR,ymfO,ymfP,ymfQ,stfP,tfaP,tfaE,pinE,mcrA
* * ÷ NC_007779_cfb 1394444 1398802–1397759 3316–4359 155 [0] [51] 53 [ycjZ]–[insH_8] [ycjZ],mppA,ynaI,[insH_8]
* * ÷ NC_007779_cfb 3972854 3988219–3987180 14327–15366 128 [2] [51] 53 [gadA]–[insH] [gadA],gadX,gadW,mdtF,mdtE,gadE,hdeD,hdeA,hdeB,yhiD,dctR,slp,[insH]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_007779_cfb 380484 =NA (NA)129 (1.070) 111/434 0.1 NA intergenic (‑418/‑46) yaiO/insC_2 hypothetical protein/IS2 insertion element repressor InsA
?NC_007779_cfb = 381407 NA (NA)coding (510/906 nt) insD IS2 insertion element transposase InsAB'
* ? NC_007779_cfb 381401 =NA (NA)125 (1.040) 105/430 0.2 NA coding (504/906 nt) insD IS2 insertion element transposase InsAB'
?NC_007779_cfb = 381812 NA (NA)coding (303/387 nt) yaiF hypothetical protein
* ? NC_007779_cfb = 13944430 (0.000)146 (1.210) 118/432 0.1 100% coding (739/900 nt) ycjZ DNA‑binding transcriptional regulator
?NC_007779_cfb 1397759 = NA (NA)intergenic (‑123/‑31) ynaI/insH_8 inner membrane protein/IS5 transposase and trans‑activator
* ? NC_007779_cfb 2519723 =NA (NA)143 (1.200) 120/428 0.1 97.3% intergenic (+33/‑54) nupC/insL nucleoside (except guanosine) transporter/transposase
?NC_007779_cfb = 4548296 4 (0.030)coding (502/549 nt) fimA major type 1 subunit fimbrin
* ? NC_007779_cfb = 2521059NA (NA)150 (1.260) 125/428 0.0 96.2% intergenic (+170/+30) insL/yfeA transposase/diguanylate cyclase
?NC_007779_cfb 4548285 = 6 (0.050)coding (491/549 nt) fimA major type 1 subunit fimbrin
* ? NC_007779_cfb = 3186081NA (NA)115 (0.960) 94/432 0.4 98.3% coding (26/2466 nt) yqiG outer membrane usher protein
?NC_007779_cfb 3512753 = 2 (0.020)coding (529/1026 nt) cytR DNA‑binding transcriptional dual regulator
* ? NC_007779_cfb = 35127591 (0.010)99 (0.820) 86/432 0.6 99.0% coding (535/1026 nt) cytR DNA‑binding transcriptional dual regulator
?NC_007779_cfb = 3744262 NA (NA)intergenic (‑90/+31) insC/yidZ IS2 element protein/DNA‑binding transcriptional regulator
* ? NC_007779_cfb 3670529 =5 (0.040)164 (1.360) 110/434 0.1 97.0% intergenic (‑79/+62) rhoL/trxA rho operon leader peptide/thioredoxin 1
?NC_007779_cfb 3746911 = NA (NA)intergenic (‑509/‑68) mdtL/insH_13 multidrug efflux system protein/IS5 element protein
* ? NC_007779_cfb = 36705320 (0.000)133 (1.100) 101/434 0.3 100% intergenic (‑82/+59) rhoL/trxA rho operon leader peptide/thioredoxin 1
?NC_007779_cfb = 3748105 NA (NA)intergenic (+146/+966) insH_13/tnaA IS5 element protein/tryptophanase/L‑cysteine desulfhydrase,PLP‑dependent
* ? NC_007779_cfb = 3744263NA (NA)145 (1.200) 113/434 0.1 98.6% intergenic (‑91/+30) insC/yidZ IS2 element protein/DNA‑binding transcriptional regulator
?NC_007779_cfb 3903274 = 2 (0.020)coding (37/825 nt) bax hypothetical protein
* ? NC_007779_cfb 3746911 =NA (NA)125 (1.030) 102/434 0.3 98.4% intergenic (‑509/‑68) mdtL/insH_13 multidrug efflux system protein/IS5 element protein
?NC_007779_cfb = 3972853 2 (0.020)coding (19/1401 nt) gadA glutamate decarboxylase A, PLP‑dependent
* ? NC_007779_cfb = 37505900 (0.000)129 (1.090) 106/426 0.2 100% intergenic (‑104/‑69) tnaA/insH_14 tryptophanase/L‑cysteine desulfhydrase,PLP‑dependent/IS5 element protein
?NC_007779_cfb 3751790 = 0 (0.000)intergenic (+151/+116) insH_14/tnaC IS5 element protein/tryptophanase leader peptide
* ? NC_007779_cfb = 39032791 (0.010)151 (1.250) 119/432 0.1 99.4% coding (42/825 nt) bax hypothetical protein
?NC_007779_cfb = 4504190 NA (NA)intergenic (+10/‑89) insD_3/yjgW IS2 insertion element transposase InsAB'/hypothetical protein
* ? NC_007779_cfb 4056220 =NA (NA)142 (1.170) 118/434 0.1 95.3% intergenic (‑209/+15) yrhD/insB Gene added/IS1 transposase InsAB'
?NC_007779_cfb 4357350 = 7 (0.060)coding (89/297 nt) yjdK hypothetical protein
* ? NC_007779_cfb 4056220 =NA (NA)133 (1.100) 117/434 0.1 95.7% intergenic (‑209/+15) yrhD/insB Gene added/IS1 transposase InsAB'
?NC_007779_cfb = 4365911 6 (0.050)coding (702/1539 nt) cadC DNA‑binding transcriptional activator
* ? NC_007779_cfb = 4056987NA (NA)148 (1.220) 128/434 0.0 100% coding (388/414 nt) yrhA hypothetical protein
?NC_007779_cfb = 4357358 0 (0.000)coding (97/297 nt) yjdK hypothetical protein
* ? NC_007779_cfb = 4056987NA (NA)144 (1.190) 117/434 0.1 97.3% coding (388/414 nt) yrhA hypothetical protein
?NC_007779_cfb 4365903 = 4 (0.030)coding (710/1539 nt) cadC DNA‑binding transcriptional activator
* ? NC_007779_cfb = 4311556NA (NA)114 (0.950) 99/432 0.3 99.1% coding (922/1029 nt) alsK D‑allose kinase
?NC_007779_cfb 4540915 = 1 (0.010)intergenic (+204/+379) yjhR/nanS frameshift suppressor/hypothetical protein