breseq  version 0.32.0b  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 684,444 G→C T9R (ACA→AGA)  insH1 ← IS5 transposase and trans‑activator
RA 1,138,811 T→C D338G (GAC→GGC)  rne ← fused ribonucleaseE: endoribonuclease/RNA‑binding protein/RNA degradosome binding protein
RA 2,346,136 G→T R4L (CGC→CTC)  glpA → sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)‑binding
RA 2,959,697 Δ1 bp coding (2097/2247 nt) ptsP ← fused PTS enzyme: PEP‑protein phosphotransferase (enzyme I)/GAF domain containing protein
RA 3,178,277 G→T E27* (GAA→TAA)  yqiC → BMFP family putative fusogenic protein
RA 4,048,586 A→T V18E (GTG→GAG)  glnG ← fused DNA‑binding response regulator in two‑component regulatory system with GlnL: response regulator/sigma54 interaction protein
RA 4,050,841 G→T P185Q (CCG→CAG)  glnA ← glutamine synthetase
RA 4,133,470 C→T A338V (GCG→GTG)  frwC → putative enzyme IIC component of PTS
RA 4,546,084 G→T H18Q (CAC→CAA yjiC ← uncharacterized protein
RA 4,596,171 G→A noncoding (48/87 nt) leuQ ← tRNA‑Leu

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 1836565 1838007 1443 64 [62] [63] 66 gdhA gdhA
* * ÷ CP009273 3347932 3352586 4655 64 [62] [63] 64 gltB–[gltD] gltB, [gltD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 360104 =NA (NA)194 (1.620) 110/296 0.0 NA intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 1973060 =8 (0.070)124 (1.030) 94/296 0.1 93.9% intergenic (‑606/‑174) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 = 3577555 NA (NA)intergenic (+15/‑1) insB1/yrhA IS1 transposase B/pseudogene, interrupted by IS1E
* ? CP009273 = 19730686 (0.050)105 (0.870) 75/296 0.6 94.6% intergenic (‑614/‑166) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 3576788 = NA (NA)intergenic (+10/‑55) yrhA/insA pseudogene, interrupted by IS1E/IS1 repressor TnpA