breseq  version 0.32.0b  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 628,974 C→G 58.0% intergenic (‑41/‑68) ybdH ← / → ybdL putative oxidoreductase/methionine aminotransferase, PLP‑dependent
RA 1,138,811 T→C 55.5% D338G (GAC→GGC)  rne ← fused ribonucleaseE: endoribonuclease/RNA‑binding protein/RNA degradosome binding protein
RA 2,277,164 A→G 13.9% L88P (CTG→CCG)  yejK ← spermidine nucleoid‑associated protein
RA 2,419,720 T→C 64.7% D183G (GAT→GGT)  hisJ ← histidine/lysine/arginine/ornithine transporter subunit
RA 2,419,933 G→T 66.4% A112D (GCC→GAC)  hisJ ← histidine/lysine/arginine/ornithine transporter subunit
RA 2,420,353 A→G 44.8% intergenic (‑86/+135) hisJ ← / ← argT histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit
RA 2,833,676 T→C 16.0% L265P (CTG→CCG)  ascF → fused cellobiose/arbutin/salicin‑specific PTS enzymes: IIB component/IC component
RA 2,943,363 G→A 54.2% V255M (GTG→ATG)  argA → fused acetylglutamate kinase homolog (inactive)/amino acid N‑acetyltransferase
JC JC 3,178,254 IS5 (+) +4 bp 33.3% coding (56‑59/291 nt) yqiC → BMFP family putative fusogenic protein
RA 3,178,277 G→T 60.8% E27* (GAA→TAA)  yqiC → BMFP family putative fusogenic protein
RA 3,219,839 C→G 42.6% R83G (CGT→GGT)  ygjI → putative transporter
RA 3,378,108:1 +C 72.2% coding (47/471 nt) argR → l‑arginine‑responsive arginine metabolism regulon transcriptional regulator
RA 4,048,586 A→T 43.9% V18E (GTG→GAG)  glnG ← fused DNA‑binding response regulator in two‑component regulatory system with GlnL: response regulator/sigma54 interaction protein
RA 4,390,178 A→C 50.3% Y25S (TAT→TCT)  hfq → global sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication
RA 4,546,084 G→T 48.0% H18Q (CAC→CAA yjiC ← uncharacterized protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 = 287112NA (NA)10 (0.490) 8/278 NT 62.5% noncoding (1/768 nt) IS1 repeat region
?CP009273 = 1973068 6 (0.290)intergenic (‑614/‑166) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
* ? CP009273 360104 =NA (NA)24 (1.170) 19/278 NT 51.1% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 23 (1.120)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 1203246 =7 (0.340)7 (0.390) 7/246 NT 46.4% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 10 (0.550)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 1973060 =7 (0.340)7 (0.340) 7/278 NT 50.0% intergenic (‑606/‑174) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 = 3577555 NA (NA)intergenic (+15/‑1) insB1/yrhA IS1 transposase B/pseudogene, interrupted by IS1E
* ? CP009273 = 2283592NA (NA)13 (0.630) 13/278 NT 56.5% noncoding (1/1195 nt) IS5 repeat region
?CP009273 = 2400260 10 (0.490)intergenic (‑140/‑780) lrhA/alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2
* ? CP009273 2871349 =35 (1.700)3 (0.160) 3/260 NT 8.6% intergenic (+372/+579) iap/ygbF aminopeptidase in alkaline phosphatase isozyme conversion/CRISPR adaptation ssRNA endonuclease
?CP009273 2871404 = 31 (1.610)intergenic (+427/+524) iap/ygbF aminopeptidase in alkaline phosphatase isozyme conversion/CRISPR adaptation ssRNA endonuclease
* ? CP009273 4532499 =9 (0.440)6 (0.310) 6/260 NT 33.9% intergenic (+49/‑433) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?CP009273 4532813 = 15 (0.780)intergenic (+363/‑119) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
* ? CP009273 = 45325079 (0.440)7 (0.360) 7/260 NT 37.4% intergenic (+57/‑425) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?CP009273 = 4532803 15 (0.780)intergenic (+353/‑129) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)