breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
JC JC 153,986 IS5 (–) +4 bp coding (1438‑1441/2598 nt) htrE ← putative outer membrane usher protein
MC JC 257,908 Δ776 bp [crl] [crl]
RA 417,391 G→C A84P (GCC→CCC)  phoB → response regulator in two‑component regulatory system with PhoR
RA 702,066 G→C P229R (CCT→CGT)  nagA ← N‑acetylglucosamine‑6‑phosphate deacetylase
RA 1,158,384 G→A W172* (TGG→TGA ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component
JC JC 1,491,904 IS186 (+) +3 bp intergenic (+54/+16) ydcA → / ← hokB putative periplasmic protein/toxic polypeptide, small
RA 1,726,507 Δ1 bp coding (485/600 nt) nemR → transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
JC JC 1,979,486 IS5 (+) +4 bp intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 2,132,787 A→C I204S (ATC→AGC)  wcaA ← putative glycosyl transferase
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,509,479 C→T intergenic (‑53/‑151) glk ← / → yfeO glucokinase/putative ion channel protein
RA 2,977,596 +C coding (998/1032 nt) galR → galactose‑inducible d‑galactose regulon transcriptional repressor; autorepressor
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
MC JC 3,815,859 Δ82 bp [rph][rph] [rph], [rph]
MC JC 3,909,312 Δ774 bp [pstA][pstC] [pstA], [pstC]
JC 4,001,645 Δ5 bp coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter
RA 4,184,543 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1293040 1306931 13892 46 [0] [1] 36 tdk–[yciU] tdk,insZ,insZ,insZ,adhE,ychE,oppA,oppB,oppC,oppD,oppF,[yciU]
* * ÷ NC_000913 2726134–2729156 2729156 1–3023 20 [19] [18] 20 [rrfG]–[rrlG] [rrfG],[rrlG]
* * ÷ NC_000913 3423749–3424234 3424526–3424238 5–778 20 [19] [18] 20 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 4110733 4111546 814 20 [19] [19] 20 tpiA tpiA
* * ÷ NC_000913 4168677–4171676 4171676 1–3000 20 [19] [18] 20 [rrlB]–[rrfB] [rrlB],[rrfB]
* * ÷ NC_000913 4210103–4213075 4213075 1–2973 22 [19] [19] 22 [rrlE]–[rrfE] [rrlE],[rrfE]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 257908 =NA (NA)46 (0.960) 41/282 0.1 100% noncoding (768/768 nt) IS1 repeat region
?NC_000913 = 1293039 0 (0.000)intergenic (‑117/‑488) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
* ? NC_000913 = 258675NA (NA)35 (0.740) 31/280 0.6 100% noncoding (1/768 nt) IS1 repeat region
?NC_000913 1306932 = 0 (0.000)coding (219/330 nt) yciU UPF0263 family protein
* ? NC_000913 1207790 =10 (0.210)33 (0.780) 29/250 0.5 76.9% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 11 (0.260)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780511 (0.230)42 (0.990) 34/250 0.2 80.2% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 11 (0.260)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related