breseq  version 0.32.0  revision 6ff6de7d1b87
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 31,061 G→A 3.1% R82H (CGC→CAC)  carB → carbamoyl‑phosphate synthase large subunit
RA 54,306 G→A 2.5% R133C (CGC→TGC)  surA ← peptidyl‑prolyl cis‑trans isomerase (PPIase)
RA 61,877 C→T 3.2% R463H (CGT→CAT)  rapA ← RNA polymerase remodeling/recycling factor ATPase, RNA polymerase‑associated, ATP‑dependent RNA translocase
RA 117,611 G→A 2.6% intergenic (‑62/+141) ppdD ← / ← nadC putative prepilin peptidase‑dependent pilin/quinolinate phosphoribosyltransferase
RA 119,226 C→G 2.5% P165R (CCC→CGC)  ampD → 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase, Zn‑dependent, murein amidase
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 279,928 A→T 4.0% intergenic (‑53/‑3) insA ← / → insI1 IS1 repressor TnpA/IS30 transposase,IS, phage, Tn, Transposon‑related functions, extrachromosomal, transposon related
RA 290,317 A→G 5.0% intergenic (‑12/‑112) argF ← / → ykgS ornithine carbamoyltransferase 2, chain F, CP4‑6 prophage/pseudogene
RA 292,102 A→G 3.1% pseudogene (699/828 nt) yagJ → CP4‑6 prophage, uncharacterized protein,Phage or Prophage Related
RA 374,944 G→T 2.8% intergenic (+63/‑515) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 374,945 A→C 2.8% intergenic (+64/‑514) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 451,338:1 +C 2.9% coding (273/948 nt) cyoA ← cytochrome o ubiquinol oxidase subunit II
RA 518,299 G→T 3.9% intergenic (‑20/+41) ybbN ← / ← ybbO DnaK co‑chaperone, thioredoxin‑like protein/putative oxidoreductase
RA 580,112 Δ1 bp 3.3% intergenic (+26/+139) ybcW → / ← ylcI DLP12 prophage, uncharacterized protein/DUF3950 family protein, DLP12 prophage
RA 580,113 Δ1 bp 3.3% intergenic (+27/+138) ybcW → / ← ylcI DLP12 prophage, uncharacterized protein/DUF3950 family protein, DLP12 prophage
RA 582,359 Δ1 bp 3.1% pseudogene (446/653 nt) ybcY ← pseudogene, DLP12 prophage, methyltransferase homology,Phage or Prophage Related
RA 582,360 Δ1 bp 3.1% pseudogene (445/653 nt) ybcY ← pseudogene, DLP12 prophage, methyltransferase homology,Phage or Prophage Related
RA 582,361 Δ1 bp 3.1% pseudogene (444/653 nt) ybcY ← pseudogene, DLP12 prophage, methyltransferase homology,Phage or Prophage Related
RA 582,362 Δ1 bp 3.1% pseudogene (443/653 nt) ybcY ← pseudogene, DLP12 prophage, methyltransferase homology,Phage or Prophage Related
RA 582,363 Δ1 bp 3.1% pseudogene (442/653 nt) ybcY ← pseudogene, DLP12 prophage, methyltransferase homology,Phage or Prophage Related
RA 582,364 Δ1 bp 3.1% pseudogene (441/653 nt) ybcY ← pseudogene, DLP12 prophage, methyltransferase homology,Phage or Prophage Related
RA 582,365 Δ1 bp 3.0% pseudogene (440/653 nt) ybcY ← pseudogene, DLP12 prophage, methyltransferase homology,Phage or Prophage Related
RA 582,366 Δ1 bp 3.0% pseudogene (439/653 nt) ybcY ← pseudogene, DLP12 prophage, methyltransferase homology,Phage or Prophage Related
RA 582,367 Δ1 bp 2.9% pseudogene (438/653 nt) ybcY ← pseudogene, DLP12 prophage, methyltransferase homology,Phage or Prophage Related
RA 582,368 Δ1 bp 3.0% pseudogene (437/653 nt) ybcY ← pseudogene, DLP12 prophage, methyltransferase homology,Phage or Prophage Related
RA 582,369 Δ1 bp 3.0% pseudogene (436/653 nt) ybcY ← pseudogene, DLP12 prophage, methyltransferase homology,Phage or Prophage Related
RA 582,370 Δ1 bp 3.0% pseudogene (435/653 nt) ybcY ← pseudogene, DLP12 prophage, methyltransferase homology,Phage or Prophage Related
RA 587,156:1 +C 2.8% coding (826/891 nt) ybcH ← PRK09936 family protein
JC 628,060 Δ3 bp 5.5% coding (367‑369/858 nt) entB → isochorismatase
RA 754,672:1 +T 4.3% intergenic (‑204/‑505) gltA ← / → sdhC citrate synthase/succinate dehydrogenase, membrane subunit, binds cytochrome b556
RA 766,051 T→A 4.9% L23Q (CTG→CAG)  mngA → fused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzymes: IIA component/IIB component/IIC component
RA 903,224 C→A 3.3% A171S (GCA→TCA)  artP ← arginine ABC transporter ATPase
RA 989,549 C→T 2.8% G270S (GGT→AGT)  pncB ← nicotinate phosphoribosyltransferase
RA 1,062,106 A→G 4.8% E103G (GAG→GGG)  torD → TorA‑maturation chaperone
RA 1,096,485 A→T 2.6% I215F (ATT→TTT)  ycdU → putative inner membrane protein
RA 1,196,305 C→T 100% R395C (CGT→TGT)  icd → isocitrate dehydrogenase, e14 prophage attachment site, tellurite reductase
RA 1,245,182 T→C 3.2% L8S (TTA→TCA)  ycgY → uncharacterized protein
RA 1,262,603 A→T 3.1% I92N (ATC→AAC)  ispE ← 4‑diphosphocytidyl‑2‑C‑methylerythritol kinase
RA 1,303,527 G→T 2.9% R210L (CGG→CTG)  oppB → oligopeptide ABC transporter permease
RA 1,350,429 C→T 2.9% R204H (CGC→CAC)  fabI ← enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent
RA 1,492,878 Δ1 bp 4.2% coding (409/1641 nt) trg → methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor
RA 1,604,356 A→G 2.8% N104D (AAC→GAC)  lsrD → autoinducer 2 import system permease protein
RA 1,840,377 Δ1 bp 2.8% coding (911/1308 nt) ynjE → molybdopterin synthase sulfurtransferase
RA 1,843,992 A→G 2.9% F294L (TTT→CTT)  ynjI ← inner membrane protein
RA 1,868,104 A→G 3.6% K399K (AAA→AAG yeaG → protein kinase, endogenous substrate unidentified, autokinase
RA 2,035,361 C→G 3.1% intergenic (+118/‑474) yedS → / → hchA pseudogene, outer membrane protein homology, putative outer membrane protein/glyoxalase III and Hsp31 molecular chaperone
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative glycosyl transferase
RA 2,136,219 A→T 3.1% V342E (GTG→GAG)  wza ← colanic acid export protein, outer membrane auxillary lipoprotein
RA 2,243,661 T→A 3.0% intergenic (‑11/‑247) folE ← / → yeiG GTP cyclohydrolase I/S‑formylglutathione hydrolase
JC 2,414,787 Δ15 bp 3.1% coding (43‑57/2145 nt) pta → phosphate acetyltransferase
RA 2,415,464 Δ1 bp 7.0% coding (720/2145 nt) pta → phosphate acetyltransferase
RA 2,423,282 T→C 3.1% K86E (AAG→GAG)  yfcI ← transposase_31 family protein
RA 2,455,420 G→T 4.1% F75L (TTC→TTA yfcV ← putative fimbrial‑like adhesin protein
RA 2,534,289 Δ1 bp 3.2% coding (226/1728 nt) ptsI → PEP‑protein phosphotransferase of PTS system (enzyme I)
RA 2,572,028 A→G 2.5% T7T (ACT→ACC eutN ← Ethanolamine catabolic microcompartment shell protein
RA 2,625,426 C→T 3.2% A105V (GCG→GTG)  ppx → exopolyphosphatase
RA 2,707,558 Δ1 bp 100% coding (242/480 nt) rseC ← SoxR iron‑sulfur cluster reduction factor component
RA 2,854,269 T→A 2.8% intergenic (+7/‑67) hypE → / → fhlA carbamoyl dehydratase, hydrogenases 1,2,3 maturation protein/formate hydrogenlyase transcriptional activator
RA 3,037,131:1 +G 2.6% coding (974/1734 nt) recJ ← ssDNA exonuclease, 5' ‑‑> 3'‑specific
RA 3,037,131:2 +C 2.6% coding (974/1734 nt) recJ ← ssDNA exonuclease, 5' ‑‑> 3'‑specific
RA 3,213,427 A→C 6.7% N128T (AAC→ACC)  rpoD → RNA polymerase, sigma 70 (sigma D) factor
RA 3,213,432 G→A 50.9% V130I (GTT→ATT)  rpoD → RNA polymerase, sigma 70 (sigma D) factor
RA 3,324,196 Δ1 bp 3.2% coding (714/849 nt) folP ← 7,8‑dihydropteroate synthase
RA 3,377,888 A→G 2.7% R106R (CGT→CGC rpsI ← 30S ribosomal subunit protein S9
RA 3,500,503 C→T 2.9% A199V (GCG→GTG)  frlA → putative fructoselysine transporter
RA 3,605,196 C→T 2.8% F73F (TTC→TTT yhhL → DUF1145 family protein
RA 3,710,552 G→T 2.9% intergenic (‑78/+246) eptB ← / ← yhjX KDO phosphoethanolamine transferase, Ca(2+)‑inducible/pyruvate‑inducible inner membrane protein, putative transporter
MC JC 3,815,858 Δ82 bp 100% [rph][rph] [rph], [rph]
RA 3,841,328 C→A 3.9% V137V (GTG→GTT nepI ← putative transporter
JC 4,001,644 Δ5 bp 100% coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter
RA 4,039,444 Δ1 bp 3.3% noncoding (1927/2905 nt) rrlA → 23S ribosomal RNA of rrnA operon
RA 4,184,542 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit
RA 4,265,710 G→A 4.8% P466P (CCG→CCA dnaB → replicative DNA helicase
RA 4,268,239 T→C 4.2% M376T (ATG→ACG)  tyrB → tyrosine aminotransferase, tyrosine‑repressible, PLP‑dependent
RA 4,295,833 T→C 7.4% intergenic (+40/+604) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,059 C→T 4.2% intergenic (+266/+378) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,154 T→C 9.2% intergenic (+361/+283) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,189 A→G 30.9% intergenic (+396/+248) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,190 A→C 29.8% intergenic (+397/+247) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,352,830 T→A 4.3% F82L (TTT→TTA ghoS → antitoxin of GhoTS toxin‑antitoxin pair, endonuclease for ghoT mRNA
RA 4,354,909 A→T 7.4% intergenic (‑191/+46) lysU ← / ← dtpC lysine tRNA synthetase, inducible/dipeptide and tripeptide permease
RA 4,397,773 T→C 3.0% W121R (TGG→CGG)  mutL → methyl‑directed mismatch repair protein
RA 4,452,306 Δ1 bp 3.1% coding (1248/1503 nt) ytfR → putative sugar ABC transporter ATPase
RA 4,541,342 G→A 2.7% G129S (GGT→AGT)  fimB → tyrosine recombinase/inversion of on/off regulator of fimA
RA 4,618,270 Δ1 bp 2.6% coding (41/1323 nt) deoA → thymidine phosphorylase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423826–3424401 3424401 1–576 54 [51] [53] 54 [rrlD] [rrlD]
* * ÷ NC_000913 4233715 4235546 1832 54 [53] [51] 54 pgi pgi

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 257908 =NA (NA)105 (0.800) 71/504 NT 100% intergenic (+9/‑768) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 = 1293039 0 (0.000)intergenic (‑117/‑488) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
* ? NC_000913 = 381689NA (NA)11 (0.090) 10/486 NT NA coding (339/366 nt)
coding (16/906 nt)
insC1
insD1
IS2 repressor TnpA
IS2 transposase TnpB
?NC_000913 = 381743 NA (NA)coding (70/906 nt) insD1 IS2 transposase TnpB
* ? NC_000913 458787 =4 (0.030)118 (0.900) 73/498 NT 96.8% intergenic (+87/‑101) clpX/lon ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
?NC_000913 608011 = NA (NA)intergenic (+23/‑54) hokE/insL1 toxic polypeptide, small/IS186 transposase
* ? NC_000913 = 4587990 (0.000)116 (0.890) 79/496 NT 100% intergenic (+99/‑89) clpX/lon ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
?NC_000913 = 609347 NA (NA)intergenic (+170/+112) insL1/entD IS186 transposase/phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex
* ? NC_000913 995022 =132 (1.000)4 (0.030) 3/488 NT 3.1% coding (967/1146 nt) ssuD alkanesulfonate monooxygenase, FMNH(2)‑dependent
?NC_000913 995153 = 120 (0.940)coding (836/1146 nt) ssuD alkanesulfonate monooxygenase, FMNH(2)‑dependent
* ? NC_000913 = 1066842107 (0.810)3 (0.020) 3/484 NT 2.8% intergenic (+16/+22) agp/yccJ glucose‑1‑phosphatase/inositol phosphatase/uncharacterized protein
?NC_000913 = 1066847 106 (0.840)intergenic (+21/+17) agp/yccJ glucose‑1‑phosphatase/inositol phosphatase/uncharacterized protein
* ? NC_000913 1105952 =114 (0.860)4 (0.030) 4/486 NT 3.5% coding (133/534 nt) ymdB O‑acetyl‑ADP‑ribose deacetylase, RNase III inhibitor during cold shock, putative cardiolipin synthase C regulatory subunit
?NC_000913 1105994 = 110 (0.860)coding (175/534 nt) ymdB O‑acetyl‑ADP‑ribose deacetylase, RNase III inhibitor during cold shock, putative cardiolipin synthase C regulatory subunit
* ? NC_000913 1207790 =67 (0.510)92 (0.740) 69/472 NT 65.8% coding (290/630 nt) stfP e14 prophage, uncharacterized protein
?NC_000913 1209619 = 33 (0.270)pseudogene (1/501 nt) stfE pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related
* ? NC_000913 = 120780561 (0.460)71 (0.570) 51/472 NT 61.2% coding (305/630 nt) stfP e14 prophage, uncharacterized protein
?NC_000913 = 1209602 33 (0.270)pseudogene (18/501 nt) stfE pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related
* ? NC_000913 1272758 =142 (1.080)3 (0.020) 3/482 NT 2.7% coding (252/696 nt) chaC cation transport regulator
?NC_000913 1273352 = 84 (0.670)coding (248/354 nt) ychN putative sulfur relay protein
* ? NC_000913 1293032 =2 (0.020)139 (1.050) 88/504 NT 98.6% intergenic (‑110/‑495) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
?NC_000913 = 1979270 NA (NA)intergenic (‑55/‑483) insA/uspC IS1 repressor TnpA/universal stress protein
* ? NC_000913 = 12994980 (0.000)74 (0.570) 54/496 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 1560542 =121 (0.920)3 (0.020) 3/500 NT 2.8% coding (388/1023 nt) ddpB D,D‑dipeptide ABC transporter permease
?NC_000913 1560822 = 89 (0.680)coding (108/1023 nt) ddpB D,D‑dipeptide ABC transporter permease
* ? NC_000913 = 1590598103 (0.780)4 (0.030) 4/486 NT 3.9% intergenic (‑62/+256) yneL/hipA pseudogene, AraC family/inactivating GltX kinase facilitating persister formation, toxin of HipAB TA pair, autokinase
?NC_000913 = 1590605 99 (0.780)intergenic (‑69/+249) yneL/hipA pseudogene, AraC family/inactivating GltX kinase facilitating persister formation, toxin of HipAB TA pair, autokinase
* ? NC_000913 = 19785020 (0.000)52 (0.410) 40/488 NT 100% intergenic (‑305/+16) flhD/insB1 flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
?NC_000913 1979279 = 0 (0.000)intergenic (‑64/‑474) insA/uspC IS1 repressor TnpA/universal stress protein
* ? NC_000913 1979486 =0 (0.000)73 (0.550) 50/504 NT 100% intergenic (‑271/‑267) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913 2101749 = NA (NA)intergenic (‑5/+146) wbbL/insH1 pseudogene, lipopolysaccharide biosynthesis protein/IS5 transposase and trans‑activator
* ? NC_000913 = 19794890 (0.000)90 (0.680) 63/504 NT 100% intergenic (‑274/‑264) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913 = 2290111 NA (NA)intergenic (‑32/+1) insH1/yejO IS5 transposase and trans‑activator/pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system
* ? NC_000913 = 2647280130 (0.980)7 (0.060) 7/484 NT 5.5% coding (44/2313 nt) pbpC penicillin‑insensitive murein repair transglycosylase, inactive transpeptidase domain protein
?NC_000913 = 2647349 117 (0.920)coding (4937/4962 nt) yfhM bacterial alpha2‑macroglobulin colonization factor ECAM, anti‑host protease defense factor, periplasmic inner membrane‑anchored lipoprotein
* ? NC_000913 = 2713827151 (1.140)5 (0.040) 5/482 NT 3.8% coding (934/1335 nt) srmB ATP‑dependent RNA helicase
?NC_000913 = 2713906 109 (0.860)coding (1013/1335 nt) srmB ATP‑dependent RNA helicase
* ? NC_000913 = 3220244149 (1.130)5 (0.040) 5/484 NT 3.2% coding (753/1380 nt) patA putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent
?NC_000913 = 3220396 164 (1.290)coding (905/1380 nt) patA putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent
* ? NC_000913 3652035 =NA (NA)124 (0.940) 72/504 NT 100% intergenic (+5/+146) yhiS/insH1 pseudogene/IS5 transposase and trans‑activator
?NC_000913 = 4049924 0 (0.000)noncoding (27/109 nt) spf Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
* ? NC_000913 = 3653229NA (NA)164 (1.240) 89/504 NT 100% intergenic (‑32/‑1) insH1/yhiS IS5 transposase and trans‑activator/pseudogene
?NC_000913 4049921 = 0 (0.000)noncoding (24/109 nt) spf Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
* ? NC_000913 4295831 =NA (NA)13 (0.120) 8/426 NT NA intergenic (+38/+606) gltP/yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
?NC_000913 = 4296395 NA (NA)noncoding (562/602 nt) RIP321 (repetitive extragenic palindromic) element, contains 11 REP sequences and 4 IHF sites RIP321 (repetitive extragenic palindromic) element, contains 11 REP sequences and 4 IHF sites
* ? NC_000913 4542683 =40 (0.300)9 (0.070) 9/486 NT 14.0% intergenic (+49/‑433) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913 4542997 = 72 (0.570)intergenic (+363/‑119) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
* ? NC_000913 = 454269138 (0.290)9 (0.070) 8/486 NT 14.2% intergenic (+57/‑425) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913 = 4542987 72 (0.570)intergenic (+353/‑129) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)