breseq  version 0.33.1  revision 8505477f25b3 mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 33 shown, sorted by frequency from high to low)
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,926,760	0	A	G	71.9%	213.4 / 113.0	128	Y82H (TAC→CAC)	asnC	DNA‑binding transcriptional dual regulator AsnC
*	NC_000913	4,063,143	0	T	C	71.2%	164.8 / 95.7	104	A299A (GCT→GCC)	yihN	putative transporter YihN
*	NC_000913	2,715,655	1	.	G	68.8%	207.2 / 105.2	80	coding (233/588 nt)	eamB	cysteine/O‑acetylserine exporter EamB
*	NC_000913	2,872,825	0	C	.	66.7%	70.4 / 108.1	60	intergenic (‑5/+189)	ftsB/ygbE	cell division protein FtsB/conserved inner membrane protein YgbE
*	NC_000913	34,111	1	.	T	61.0%	252.9 / 164.6	101	intergenic (+73/‑189)	carB/caiF	carbamoyl phosphate synthetase subunit beta/DNA‑binding transcriptional activator CaiF
*	NC_000913	143,103	0	G	A	54.8%	56.3 / 182.7	126	E109K (GAG→AAG)	yadG	putative ABC transporter ATP‑binding protein YadG
*	NC_000913	2,342,616	0	G	A	48.2%	16.3 / 187.5	114	V518V (GTC→GTT)	yfaL	putative autotransporter adhesin YfaL
*	NC_000913	4,296,060	0	C	T	31.9%	327.4 / 256.7	241	intergenic (+266/+376)	gltP/yjcO	glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*	NC_000913	3,499,600	0	T	G	31.9%	76.9 / 14.9	48	G51G (GGT→GGG)	yhfL	DUF4223 domain‑containing lipoprotein YhfL
*	NC_000913	624,589	0	T	C	31.3%	233.2 / 143.9	150	intergenic (‑79/‑296)	fepB/entC	ferric enterobactin ABC transporter periplasmic binding protein/isochorismate synthase EntC
*	NC_000913	1,684,749	0	A	C	29.7%	159.6 / 14.0	74	D164A (GAC→GCC)	tus	DNA replication terminus site‑binding protein
*	NC_000913	888,014	0	T	G	28.7%	199.8 / 16.9	94	noncoding (44/81 nt)	rybB	small regulatory RNA RybB
*	NC_000913	2,483,268	0	T	A	28.6%	167.2 / 32.7	78	L24F (TTA→TTT)	emrK	tripartite efflux pump membrane fusion protein EmrK
*	NC_000913	954,398	0	A	C	27.9%	129.1 / 11.2	62	G23G (GGT→GGG)	focA	formate channel FocA
*	NC_000913	1,489,174	0	G	T	27.7%	225.8 / 28.0	83	G315C (GGT→TGT)	aldA	aldehyde dehydrogenase A
*	NC_000913	1,015,516	0	G	T	25.6%	199.7 / 31.0	82	intergenic (+57/‑199)	pqiC/rmf	intermembrane transport lipoprotein PqiC/ribosome modulation factor
*	NC_000913	2,029,274	0	C	T	25.0%	132.9 / 12.6	60	K31K (AAG→AAA)	yedI	conserved inner membrane protein YedI
*	NC_000913	1,775,467	0	A	C	24.7%	211.7 / 10.7	97	intergenic (+23/‑120)	aroD/ydiF	3‑dehydroquinate dehydratase/putative acetate‑CoA transferase
*	NC_000913	3,180,341	0	G	T	24.6%	140.6 / 19.4	61	G200C (GGT→TGT)	ygiB	DUF1190 domain‑containing protein YgiB
*	NC_000913	4,457,105	0	A	G	24.2%	227.5 / 23.1	91	I441V (ATC→GTC)	mpl	UDP‑N‑acetylmuramate‑‑L‑alanyl‑gamma‑D‑glutamyl‑ meso‑2,6‑diaminoheptanedioate ligase

Marginal new junction evidence (sorted from low to high skew)
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3875268	154 (1.350)	120 (1.440) +CGCGCAGGAAACA	5/72	8.3	56.9%	intergenic (‑108/‑170)	dgoR/yidX	putative DNA‑binding transcriptional regulator DgoR/putative lipoprotein YidX
	?	NC_000913	4641321 =	95 (0.830)					coding (380/687 nt)	yjtD	putative rRNA methyltransferase