breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 16,923 T→G 5.2% K13T (AAG→ACG)  mokC ← regulatory protein for HokC, overlaps CDS of hokC
RA 83,587 A→C 5.7% intergenic (‑58/+35) leuA ← / ← leuL 2‑isopropylmalate synthase/leu operon leader peptide
RA 143,533 T→C 5.7% L252P (CTG→CCG)  yadG → putative ABC transporter ATPase
RA 151,519 A→C 6.6% I27M (ATT→ATG yadK ← putative fimbrial‑like adhesin protein
RA 151,522 G→T 6.8% A26A (GCC→GCA yadK ← putative fimbrial‑like adhesin protein
RA 246,771 G→A 5.1% P20P (CCG→CCA yafL → putative lipoprotein and C40 family peptidase
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 810,569 G→A 11.5% Q63Q (CAG→CAA bioF → 8‑amino‑7‑oxononanoate synthase
RA 1,041,937 T→G 7.0% intergenic (+22/+93) appA → / ← etk phosphoanhydride phosphorylase/tyrosine‑protein kinase, role in O‑antigen capsule formation
RA 1,181,724 A→G 5.4% M1T (ATG→ACG) † ymfA ← DUF3592 family inner membrane protein
RA 1,240,929 T→C 7.2% T381T (ACA→ACG cvrA ← putative cation/proton antiporter
MC JC 1,299,499 Δ1,199 bp 100% intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
RA 1,413,542 A→T 100% Y67N (TAT→AAT)  ydaC ← DUF1187 family protein, Rac prophage; putative double‑strand break reduction protein
RA 1,793,897 Δ1 bp 100% coding (213/552 nt) btuE ← glutathione peroxidase
RA 1,883,027 C→G 7.2% intergenic (+30/‑161) dmlA → / → yeaV D‑malate oxidase, NAD‑dependent; putative tartrate dehydrogenase/putative transporter
RA 1,901,435 A→C 7.1% L51V (TTA→GTA)  yoaE ← putative membrane protein/conserved protein
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 2,093,040 G→A 100% A215T (GCT→ACT)  hisC → histidinol‑phosphate aminotransferase
RA 2,291,234 C→G 5.0% intergenic (+89/+122) narP → / ← ccmH response regulator in two‑component regulatory system with NarQ/heme lyase, CcmH subunit
RA 3,156,588 C→A 7.1% intergenic (+72/‑33) yqhD → / → dkgA aldehyde reductase, NADPH‑dependent/2,5‑diketo‑D‑gluconate reductase A
RA 3,156,589 T→G 7.1% intergenic (+73/‑32) yqhD → / → dkgA aldehyde reductase, NADPH‑dependent/2,5‑diketo‑D‑gluconate reductase A
RA 3,368,171 C→G 6.2% P116R (CCG→CGG)  yhcG → DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
RA 3,456,014 C→A 5.5% H147N (CAT→AAT)  gspC → general secretory pathway component, cryptic
RA 3,569,436 T→C 6.2% H628R (CAC→CGC)  glgX ← glycogen debranching enzyme
RA 3,833,667 G→T 5.6% I290I (ATC→ATA yicI ← putative alpha‑glucosidase
RA 3,835,243 T→C 5.2% Q229R (CAG→CGG)  yicJ ← putative transporter
RA 4,094,414 T→G 5.4% intergenic (‑143/+308) rhaD ← / ← rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
RA 4,117,004 T→A 100% D73V (GAT→GTT)  glpK ← glycerol kinase
MC JC 4,188,484 Δ27 bp 100% coding (3136‑3162/4224 nt) rpoC → RNA polymerase, beta prime subunit
RA 4,307,295 A→T 7.5% L169Q (CTG→CAG)  alsK ← D‑allose kinase
RA 4,307,299 G→A 7.8% P168S (CCC→TCC)  alsK ← D‑allose kinase

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 399794 =48 (0.740)3 (0.050) 3/262 NT 5.9% coding (202/213 nt) yaiZ DUF2754 family putative inner membrane protein
?NC_000913 399823 = 51 (0.830)intergenic (+18/+6) yaiZ/ddlA DUF2754 family putative inner membrane protein/D‑alanine‑D‑alanine ligase A
* ? NC_000913 1207790 =37 (0.570)22 (0.380) 21/244 NT 43.7% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 24 (0.420)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780538 (0.580)26 (0.450) 24/244 NT 47.5% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 24 (0.420)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 2304469 =56 (0.860)3 (0.050) 3/262 NT 6.1% noncoding (55/655 nt) REP161 (repetitive extragenic palindromic) element; contains 12 REP sequences REP161 (repetitive extragenic palindromic) element; contains 12 REP sequences
?NC_000913 2304953 = 39 (0.630)noncoding (539/655 nt) REP161 (repetitive extragenic palindromic) element; contains 12 REP sequences REP161 (repetitive extragenic palindromic) element; contains 12 REP sequences
* ? NC_000913 3750789 =85 (1.310)4 (0.070) 4/260 NT 5.1% intergenic (+9/+23) sgbE/ysaC L‑ribulose‑5‑phosphate 4‑epimerase/pseudogene
?NC_000913 3750811 = 70 (1.140)intergenic (+31/+1) sgbE/ysaC L‑ribulose‑5‑phosphate 4‑epimerase/pseudogene
* ? NC_000913 = 3944977NA (NA)7 (0.110) 7/264 NT 15.2% noncoding (1275/2904 nt) rrlC 23S ribosomal RNA of rrnC operon
?NC_000913 = 4038810 41 (0.630)noncoding (1293/2905 nt) rrlA 23S ribosomal RNA of rrnA operon
* ? NC_000913 = 417180256 (0.860)7 (0.110) 5/272 NT 12.2% intergenic (+47/‑254) rrfB/murB 5S ribosomal RNA of rrnB operon/UDP‑N‑acetylenolpyruvoylglucosamine reductase, FAD‑binding
?NC_000913 = 4213161 46 (0.720)intergenic (+3/‑72) rrfE/yjaA 5S ribosomal RNA of rrnE operon/stress‑induced protein