breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 1,105,093 T→C 7.1% S45P (TCC→CCC)  csgC → curli assembly protein
RA 1,205,173 A→G 5.5% pseudogene (770/1134 nt) aaaE → pseudogene, phage terminase protein A family, e14 prophage;Phage or Prophage Related
RA 1,286,965 A→G 14.4% intergenic (+439/+101) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,298,252 T→C 6.3% intergenic (‑131/‑346) adhE ← / → ychE fused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase/UPF0056 family inner membrane protein
RA 1,413,542 A→T 100% Y67N (TAT→AAT)  ydaC ← DUF1187 family protein, Rac prophage; putative double‑strand break reduction protein
RA 1,706,969 C→T 6.7% N17N (AAC→AAT rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,793,897 Δ1 bp 100% coding (213/552 nt) btuE ← glutathione peroxidase
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 2,093,040 G→A 100% A215T (GCT→ACT)  hisC → histidinol‑phosphate aminotransferase
RA 2,270,600 T→C 8.5% intergenic (+57/‑124) mepS → / → rtn murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein, weak murein LD‑carboxypeptidase/resistance protein for phages lambda and N4, putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
RA 2,340,717 C→T 5.3% V1151M (GTG→ATG)  yfaL ← adhesin
RA 2,388,341 A→G 5.2% T28T (ACT→ACC yfbN ← uncharacterized protein
RA 2,394,768 T→C 13.4% T93A (ACT→GCT)  nuoL ← NADH:ubiquinone oxidoreductase, membrane subunit L
RA 2,473,882 T→G 13.1% L94R (CTG→CGG)  yfdP → CPS‑53 (KpLE1) prophage; uncharacterized protein
RA 2,501,610 G→A 5.6% L161L (CTG→CTA ypdC → putative DNA‑binding protein
RA 2,525,756 A→G 6.5% N211S (AAT→AGT)  yfeN → putative outer membrane protein
RA 2,565,055 C→G 5.8% Q179E (CAG→GAG)  yffS → CPZ‑55 prophage; uncharacterized protein
RA 2,818,143 T→C 10.2% intergenic (‑10/+53) argZ ← / ← argY tRNA‑Arg/tRNA‑Arg
RA 2,980,742 A→T 7.3% intergenic (‑109/+20) ygeA ← / ← araE Asp/Glu_racemase family protein/arabinose transporter
RA 3,358,324 G→A 5.0% C1201Y (TGC→TAC)  gltB → glutamate synthase, large subunit
RA 3,436,814 G→A 5.8% R100H (CGC→CAC)  trkA → NAD‑binding component of TrK potassium transporter
RA 3,465,130 T→C 6.4% V17V (GTT→GTC gspM → general secretory pathway component, cryptic
RA 3,481,009 C→A 7.8% D51Y (GAT→TAT)  kefG ← potassium‑efflux system ancillary protein for KefB, glutathione‑regulated
RA 4,085,619 G→T 7.7% A68E (GCA→GAA)  fdoG ← formate dehydrogenase‑O, large subunit
RA 4,116,406 C→T 7.1% M272I (ATG→ATA glpK ← glycerol kinase
RA 4,117,004 T→A 26.2% D73V (GAT→GTT)  glpK ← glycerol kinase
RA 4,117,050 T→G 44.9% S58R (AGC→CGC)  glpK ← glycerol kinase
RA 4,182,928 A→T 12.2% E562V (GAA→GTA)  rpoB → RNA polymerase, beta subunit
RA 4,187,597 C→T 92.0% P750L (CCG→CTG)  rpoC → RNA polymerase, beta prime subunit
RA 4,279,915 A→G 6.2% intergenic (+88/‑64) ghxP → / → yjcE guanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter/putative cation/proton antiporter
RA 4,325,949 A→G 6.0% intergenic (‑207/+451) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,356,446 A→T 7.1% intergenic (‑34/+25) dtpC ← / ← cadA dipeptide and tripeptide permease/lysine decarboxylase, acid‑inducible
RA 4,356,447 A→T 6.4% intergenic (‑35/+24) dtpC ← / ← cadA dipeptide and tripeptide permease/lysine decarboxylase, acid‑inducible
RA 4,491,466 G→A 5.3% A354V (GCC→GTC)  idnT ← L‑idonate and D‑gluconate transporter
RA 4,544,588 G→A 7.2% G95E (GGA→GAA)  fimC → periplasmic chaperone

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 224344NA (NA)3 (0.040) 3/270 NT NA noncoding (574/1542 nt) rrsH 16S ribosomal RNA of rrnH operon
?NC_000913 = 224358 NA (NA)noncoding (588/1542 nt) rrsH 16S ribosomal RNA of rrnH operon
* ? NC_000913 1207790 =25 (0.300)45 (0.620) 37/244 NT 64.2% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 28 (0.380)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780523 (0.280)63 (0.870) 52/244 NT 72.3% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 28 (0.380)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)72 (0.900) 59/268 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 2729041NA (NA)6 (0.080) 6/262 NT NA noncoding (142/2904 nt) rrlG 23S ribosomal RNA of rrnG operon
?NC_000913 = 2729045 NA (NA)noncoding (138/2904 nt) rrlG 23S ribosomal RNA of rrnG operon
* ? NC_000913 2729473 =53 (0.640)4 (0.050) 3/256 NT 7.9% intergenic (‑31/+141) gltW/rrsG tRNA‑Glu/16S ribosomal RNA of rrnG operon
?NC_000913 = 4168362 44 (0.580)intergenic (+163/‑9) rrsB/gltT 16S ribosomal RNA of rrnB operon/tRNA‑Glu
* ? NC_000913 2729953 =NA (NA)3 (0.040) 4/264 NT NA noncoding (1203/1542 nt) rrsG 16S ribosomal RNA of rrnG operon
?NC_000913 2729970 = NA (NA)noncoding (1186/1542 nt) rrsG 16S ribosomal RNA of rrnG operon