breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 49,765 C→T intergenic (+134/‑58) kefC → / → folA K(+) : H(+) antiporter KefC/dihydrofolate reductase
RA 49,884 C→T P21L (CCG→CTG)  folA → dihydrofolate reductase
RA 49,905 T→G L28R (CTC→CGC)  folA → dihydrofolate reductase
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 481,400 C→T A1002T (GCA→ACA)  acrB ← multidrug efflux pump RND permease AcrB
RA 485,614 G→T N2K (AAC→AAA acrA ← multidrug efflux pump membrane fusion lipoprotein AcrA
RA 803,662 C→A L54I (CTT→ATT)  ybhJ → putative hydratase YbhJ
RA 1,905,761 G→A G25D (GGT→GAT)  mntP → Mn(2(+)) exporter
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 4,182,820 C→T H526Y (CAC→TAC)  rpoB → RNA polymerase subunit beta
RA 4,390,754 G→T A252A (GCC→GCA rsgA ← ribosome small subunit‑dependent GTPase A

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423711–3424234 3424593–3424238 5–883 7 [6] [6] 7 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 4296108–4296378 4296398 21–291 9 [5] [4] 18 gltP/yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)68 (1.520) 33/120 0.0 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 160379833 (0.690)10 (0.210) 7/128 2.0 23.0% coding (780/1029 nt) lsrC Autoinducer‑2 ABC transporter membrane subunit LsrC
?NC_000913 1603802 = 34 (0.710)coding (784/1029 nt) lsrC Autoinducer‑2 ABC transporter membrane subunit LsrC