breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
JC JC 154,864 IS5 (+) +4 bp coding (560‑563/2598 nt) htrE ← putative fimbrial usher protein HtrE
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 559,427 G→T A152S (GCG→TCG)  sfmC → putative fimbrial chaperone SfmC
RA 656,302 C→A intergenic (‑334/‑255) dcuC ← / → pagP anaerobic C4‑dicarboxylate transporter DcuC/Lipid IVA palmitoyltransferase
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 975,824 C→A A68E (GCA→GAA)  mukE → chromosome partitioning protein MukE
RA 1,045,723 C→A Q27H (CAG→CAT gfcE ← putative exopolysaccharide export lipoprotein GfcE
JC 1,198,505 (AATGATGA)6→7 intergenic (‑268/‑190) ymfE ← / → lit e14 prophage; uncharacterized protein YmfE/e14 prophage; cell death peptidase Lit
RA 1,488,673 G→T G148C (GGC→TGC)  aldA → aldehyde dehydrogenase A
RA 1,552,032 C→A intergenic (+41/+366) fdnI → / ← yddM formate dehydrogenase N subunit gamma/putative DNA‑binding transcriptional regulator YddM
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,720,688 T→C D46G (GAC→GGC)  slyA ← DNA‑binding transcriptional dual regulator SlyA
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,443,760 A→C D460A (GAC→GCC)  tamA → translocation and assembly module subunit TamA
RA 4,631,375 G→T A215S (GCC→TCC)  slt → soluble lytic murein transglycosylase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102964 3104061 1098 51 [50] [49] 51 [mutY] [mutY]
* * ÷ NC_000913 3423766–3424234 3424523–3424238 5–758 51 [49] [40] 60 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 3944157–3945567 3946841–3946693 1127–2685 52 [49] [49] 51 [rrlC]–rrfC [rrlC],rrfC

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =196 (1.140)100 (0.660) 61/244 0.7 38.5% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 146 (0.960)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 1207805196 (1.140)51 (0.330) 37/244 2.2 24.2% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 146 (0.960)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)228 (1.360) 103/268 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =220 (1.280)282 (1.650) 92/274 0.2 71.6% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 5 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)219 (1.270) 96/276 0.1 96.0% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 9 (0.050)coding (3907/4134 nt) rhsA rhs element protein RhsA