breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 583,642 A→G intergenic (+582/‑39) tfaX → / → appY DLP12 prophage; protein TfaX/DNA‑binding transcriptional activator AppY
RA 809,091 A→G I56T (ATC→ACC)  bioA ← adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,290,964 G→T L241F (TTG→TTT rssB → regulator of RpoS
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
JC 2,048,724 Δ5 bp coding (3787‑3791/7077 nt) yeeJ → inverse autotransporter adhesin
RA 2,128,377 G→T P171H (CCC→CAC)  wcaF ← putative acyl transferase
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,327,705 G→T L147I (CTC→ATC)  yfaP ← DUF2135 domain‑containing protein YfaP
RA 2,411,113 T→C G80G (GGA→GGG yfbS ← putative transporter YfbS
RA 2,455,877 Δ1 bp intergenic (‑231/+450) yfcV ← / ← sixA putative fimbrial protein YfcV/phosphohistidine phosphatase
RA 2,870,062 G→T C71* (TGC→TGA umpG ← broad specificity 5'(3')‑nucleotidase and polyphosphatase
RA 3,036,538 C→A P523P (CCG→CCT recJ ← ssDNA‑specific exonuclease RecJ
RA 3,061,836 T→C D329D (GAT→GAC scpA → methylmalonyl‑CoA mutase
RA 3,326,714 A→C D74E (GAT→GAG ftsH ← ATP‑dependent zinc metalloprotease FtsH
RA 3,336,462 C→A K11N (AAG→AAT murA ← UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,510,138 A→G L280S (TTA→TCA)  yhfX ← putative PLP‑binding protein YhfX
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,734,694 C→A D325E (GAC→GAA xylH → xylose ABC transporter membrane subunit
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102986 3104052 1067 33 [30] [29] 31 [mutY] [mutY]
* * ÷ NC_000913 3423770–3424233 3424525–3424238 6–756 31 [20] [29] 41 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =57 (0.460)109 (1.000) 60/244 0.3 65.8% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 63 (0.580)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780557 (0.460)65 (0.600) 39/244 1.1 53.4% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 63 (0.580)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)178 (1.490) 82/268 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =219 (1.780)202 (1.650) 73/274 0.2 64.6% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 4 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)131 (1.060) 68/276 0.3 92.9% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 10 (0.080)coding (3907/4134 nt) rhsA rhs element protein RhsA