breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 389,699 C→A R10L (CGC→CTC)  hemB ← porphobilinogen synthase
RA 656,330 A→G intergenic (‑362/‑227) dcuC ← / → pagP anaerobic C4‑dicarboxylate transporter DcuC/Lipid IVA palmitoyltransferase
RA 855,922 C→A intergenic (‑178/‑41) ldtB ← / → ybiT L,D‑transpeptidase YbiS/ABC transporter ATP‑binding protein YbiT
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 922,738 G→T intergenic (‑148/‑175) cspD ← / → clpS DNA replication inhibitor/specificity factor for ClpA‑ClpP chaperone‑protease complex
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,234,451 C→A V89V (GTG→GTT nhaB ← Na(+):H(+) antiporter NhaB
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,151,853 G→T P207Q (CCG→CAG)  pphC ← protein‑serine/threonine phosphatase PphC
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,266,937 C→A intergenic (+76/‑177) alr → / → tyrB alanine racemase 1/tyrosine aminotransferase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,367,748 C→A D194Y (GAC→TAC)  aspA ← aspartate ammonia‑lyase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196245 1211428 15184 14 [10] [13] 16 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3102959 3104049 1091 14 [13] [13] 14 [mutY] [mutY]
* * ÷ NC_000913 3423791–3424234 3424536–3424239 6–746 15 [9] [13] 14 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 374921 =NA (NA)34 (0.930) 13/190 1.5 NA intergenic (+40/‑538) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377531 = NA (NA)intergenic (+220/+4) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 374963NA (NA)21 (0.580) 13/190 1.5 NA noncoding (33/34 nt) other REP31a
?NC_000913 = 377487 NA (NA)intergenic (+176/+48) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 375102 =NA (NA)29 (0.700) 19/216 1.0 NA noncoding (17/36 nt) other REP31d
?NC_000913 377351 = NA (NA)noncoding (16/37 nt) other REP32a
* ? NC_000913 = 12994980 (0.000)61 (1.180) 44/268 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =69 (1.300)66 (1.250) 45/274 0.1 65.8% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 0 (0.000)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)55 (1.040) 40/276 0.3 98.2% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 1 (0.020)coding (3907/4134 nt) rhsA rhs element protein RhsA