breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 131,531 C→A intergenic (‑271/‑84) yacH ← / → acnB DUF3300 domain‑containing protein YacH/hypothetical protein
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 409,980 G→T R14S (CGC→AGC)  rdgC ← nucleoid‑associated protein RdgC
RA 474,257 C→A intergenic (+5/+44) amtB → / ← tesB ammonia/ammonium transporter/acyl‑CoA thioesterase II
RA 718,705 G→T R586S (CGC→AGC)  speF ← ornithine decarboxylase, degradative
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,342,124 C→A I68I (ATC→ATA pyrF → orotidine‑5'‑phosphate decarboxylase
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,233,498 C→A V207V (GTC→GTA sanA → DUF218 domain‑containing protein SanA
RA 2,579,384 G→T R240L (CGA→CTA)  talA → transaldolase A
JC 2,765,463 (TATGGCAC)6→7 intergenic (‑310/+50) abpA ← / ← yfjM CP4‑57 prophage; anti‑bacteriophage protein/CP4‑57 prophage; protein YfjM
RA 3,238,885 C→A S102R (AGC→AGA alx → putative membrane‑bound redox modulator Alx
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,494,239 C→A L77M (CTG→ATG)  nirB → nitrite reductase catalytic subunit NirB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,505,167 C→A G370G (GGC→GGA yjhB → putative sialic acid transporter

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102975 3104095 1121 23 [22] [21] 23 mutY–[yggX] mutY,[yggX]
* * ÷ NC_000913 3423753–3424234 3424534–3424238 5–782 24 [22] [22] 23 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 374963NA (NA)27 (0.520) 13/190 2.5 NA noncoding (33/34 nt) other REP31a
?NC_000913 = 377487 NA (NA)intergenic (+176/+48) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 12994980 (0.000)69 (0.940) 51/268 0.2 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =124 (1.630)119 (1.580) 71/274 0.0 65.7% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 1 (0.010)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)107 (1.410) 67/276 0.0 97.3% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 3 (0.040)coding (3907/4134 nt) rhsA rhs element protein RhsA