breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 595,933 G→T G112C (GGT→TGT)  cusC → copper/silver export system outer membrane channel
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 936,953 G→T D1244Y (GAT→TAT)  ftsK → cell division DNA translocase FtsK
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,085,013 C→A E171D (GAG→GAT yeeE ← inner membrane protein YeeE
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,496,532 G→T T296T (ACG→ACT lpxP → palmitoleoyl acyltransferase
RA 2,811,850 G→T D142Y (GAC→TAC)  emrA → multidrug efflux pump membrane fusion protein EmrA
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
JC JC 3,744,255 IS5 (+) +4 bp intergenic (+45/‑70) yiaL → / → yiaM DUF386 domain‑containing protein YiaL/2,3‑diketo‑L‑gulonate:Na(+) symporter ‑ membrane subunit
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,966,153 C→A intergenic (+63/‑78) trxA → / → rhoL thioredoxin 1/rho operon leader peptide
MC JC 4,059,453 Δ9 bp coding (1047‑1055/1824 nt) bipA → ribosome‑dependent GTPase, ribosome assembly factor
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196275 1211433 15159 31 [25] [23] 30 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3102972 3104066 1095 30 [29] [29] 30 mutY mutY
* * ÷ NC_000913 3423761–3424234 3424533–3424239 6–773 30 [29] [29] 32 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)105 (1.040) 65/268 0.2 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =147 (1.410)174 (1.680) 88/274 0.0 70.2% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 2 (0.020)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)122 (1.170) 63/276 0.3 95.3% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 6 (0.060)coding (3907/4134 nt) rhsA rhs element protein RhsA