breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 363,940 G→T P789Q (CCG→CAG)  lacZ ← beta‑galactosidase
RA 553,507 G→T P145Q (CCG→CAG)  lpxH ← UDP‑2,3‑diacylglucosamine diphosphatase
JC JC 571,356 IS2 (–) +5 bp coding (464‑468/552 nt) ybcL → DLP12 prophage; periplasmic protein YbcL
RA 644,345 C→A C220F (TGC→TTC)  rna ← RNase I
RA 728,468 G→T L89I (CTT→ATT)  kdpA ← K(+) transporting P‑type ATPase subunit KdpA
RA 744,292 G→T G17V (GGT→GTT)  pxpC → 5‑oxoprolinase component C
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,360,746 G→T R55S (CGT→AGT)  puuA ← glutamate‑putrescine ligase
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,893,777 C→A intergenic (+66/‑356) yoaB → / → yoaC RutC family protein YoaB/DUF1889 domain‑containing protein YoaC
RA 1,948,841 G→A A561V (GCG→GTG)  aspS ← aspartate‑‑tRNA ligase
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 3,080,385 A→T I417I (ATT→ATA tktA ← transketolase 1
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,925,213 G→T Q141K (CAA→AAA)  mnmG ← 5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG
RA 4,083,225 G→T N866K (AAC→AAA fdoG ← formate dehydrogenase O subunit alpha
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102938 3104083 1146 28 [27] [27] 28 mutY mutY
* * ÷ NC_000913 3423785–3424234 3424535–3424238 5–751 29 [20] [26] 28 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =119 (1.120)27 (0.290) 22/244 2.5 22.0% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 86 (0.920)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)103 (1.000) 70/268 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =137 (1.300)169 (1.610) 77/274 0.1 70.6% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 5 (0.050)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)125 (1.180) 85/276 0.0 96.9% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 4 (0.040)coding (3907/4134 nt) rhsA rhs element protein RhsA