breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 689,521 G→T P454Q (CCG→CAG)  lnt ← apolipoprotein N‑acyltransferase
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 941,170 G→T C71F (TGC→TTC)  dmsA → dimethyl sulfoxide reductase subunit A
RA 1,068,965 G→T A292E (GCA→GAA)  rutG ← pyrimidine:H(+) symporter
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,417,724 C→A D14Y (GAC→TAC)  racC ← Rac prophage; protein RacC
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,657,212 C→A G575V (GGC→GTC)  hscA ← iron‑sulfur cluster biosynthesis chaperone HscA
RA 2,729,307 C→A intergenic (‑123/+62) rrlG ← / ← gltW 23S ribosomal RNA/tRNA‑Glu
RA 2,967,075 G→T R454S (CGT→AGT)  ptsP ← phosphoenolpyruvate‑protein phosphotransferase PtsP
RA 2,984,070 C→A G19W (GGG→TGG)  kduI ← 5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase
RA 2,999,269 G→T A208D (GCT→GAT)  ygeR ← LysM domain‑containing putative peptidase lipoprotein YgeR
RA 3,031,465 C→A L33L (CTC→CTA uacT → urate:H(+) symporter
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,548,380 C→A G564V (GGC→GTC)  malQ ← 4‑alpha‑glucanotransferase
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,595,421 C→A A20A (GCG→GCT livM ← branched chain amino acid/phenylalanine ABC transporter membrane subunit LivM
RA 3,595,754 G→T A217E (GCG→GAG)  livH ← branched chain amino acid/phenylalanine ABC transporter membrane subunit LivH
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,031,719 G→T A187S (GCG→TCG)  pepQ → Xaa‑Pro dipeptidase
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196270 1211432 15163 28 [21] [27] 28 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3102949 3104065 1117 31 [27] [27] 28 [mutY] [mutY]
* * ÷ NC_000913 3423770–3424530 3424530 1–761 29 [26] [26] 28 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 374963NA (NA)28 (0.380) 14/190 2.9 NA noncoding (33/34 nt) other REP31a
?NC_000913 = 377487 NA (NA)intergenic (+176/+48) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 12994980 (0.000)97 (0.930) 60/268 0.3 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =156 (1.450)185 (1.730) 76/274 0.1 70.4% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 1 (0.010)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)127 (1.180) 69/276 0.2 97.7% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 3 (0.030)coding (3907/4134 nt) rhsA rhs element protein RhsA