breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 295,424 G→T R52R (CGC→CGA yagN ← CP4‑6 prophage; protein YagN
RA 311,480 (T)8→7 intergenic (‑144/‑632) ecpR ← / → ykgL DNA‑binding transcriptional dual regulator MatA/uncharacterized protein YkgL
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,231,752 G→T R189R (CGG→CGT umuC → DNA polymerase V catalytic protein
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,839,083 G→T A113S (GCC→TCC)  ynjD → putative ABC transporter ATP‑binding protein YnjD
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,972,935 G→T G135G (GGC→GGA cheW ← chemotaxis protein CheW
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,188,569 C→A G781V (GGC→GTC)  yehB ← putative fimbrial usher protein YehB
RA 2,218,525 C→A A11A (GCG→GCT yehY ← glycine betaine ABC transporter membrane subunit YehY
RA 2,892,508 G→T V99F (GTT→TTT)  queD → 6‑carboxy‑5,6,7,8‑tetrahydropterin synthase
JC 3,306,862 (GTACGACCG)1→2 coding (999/1890 nt) deaD ← ATP‑dependent RNA helicase DeaD
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,401,914 A→G R473R (CGT→CGC csrD ← regulator of CsrB and CsrC decay
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,708,837 C→A E547* (GAA→TAA)  eptB ← Kdo2‑lipid A phosphoethanolamine 7''‑transferase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,446,409 G→T E766D (GAG→GAT tamB → translocation and assembly module subunit TamB

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196270 1211431 15162 37 [33] [31] 38 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3102971 3104078 1108 35 [33] [33] 35 mutY mutY
* * ÷ NC_000913 3423770–3424544 3424544 1–775 38 [31] [33] 35 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)199 (1.600) 100/268 0.0 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =216 (1.690)198 (1.560) 79/274 0.1 64.3% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 5 (0.040)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)150 (1.170) 74/276 0.2 98.0% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 3 (0.020)coding (3907/4134 nt) rhsA rhs element protein RhsA