breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 312,360 C→A intergenic (+21/+14) ykgL → / ← ykgO uncharacterized protein YkgL/putative ribosomal protein
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,034,143 C→A S297Y (TCT→TAT)  hyaB → hydrogenase 1 large subunit
RA 1,165,990 C→A intergenic (+305/‑95) ycfP → / → ndh UPF0227 protein YcfP/NADH:quinone oxidoreductase II
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,619,121 T→C M1A (GTG→GCG) † marR → DNA‑binding transcriptional repressor MarR
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,171,892 G→T Q328K (CAG→AAG)  gatD ← galactitol‑1‑phosphate 5‑dehydrogenase
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,563,695 C→A S35* (TCA→TAA)  yffQ → CPZ‑55 prophage; uncharacterized protein YffQ
RA 3,100,348 G→T C127* (TGC→TGA ansB ← L‑asparaginase 2
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
MC JC 3,650,693 Δ1,343 bp IS5‑mediated [yhiS] [yhiS]
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,867,789 G→T C21F (TGC→TTC)  yidQ → DUF1375 domain‑containing putative lipoprotein YidQ
RA 4,216,697 C→A A407E (GCA→GAA)  aceB → malate synthase A
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196255 1211453 15199 17 [14] [16] 18 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3102986 3104055 1070 19 [15] [13] 17 [mutY] [mutY]
* * ÷ NC_000913 3423766–3424234 3424544–3424240 7–779 19 [16] [16] 18 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3363001 =79 (1.400)85 (1.520) 51/276 0.1 68.2% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 1 (0.020)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)58 (1.030) 48/278 0.1 96.7% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 2 (0.040)coding (3907/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 4542682 =38 (0.670)12 (0.230) 12/260 3.0 23.2% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 44 (0.830)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit