breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,197,761 T→A E159D (GAA→GAT ymfE ← e14 prophage; uncharacterized protein YmfE
RA 1,645,276 G→T intergenic (‑2/+70) hokD ← / ← relE Qin prophage; toxic protein HokD/Qin prophage; mRNA interferase toxin RelE
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,075,358 C→A L194M (CTG→ATG)  yeeR → CP4‑44 prophage; inner membrane protein YeeR
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,211,645 G→T E141* (GAA→TAA)  yehR → DUF1307 domain‑containing lipoprotein YehR
RA 2,215,391 A→T T176S (ACC→TCC)  mlrA → DNA‑binding transcriptional activator MlrA
RA 2,232,269 T→C G142G (GGT→GGC cdd → cytidine/deoxycytidine deaminase
RA 2,260,484 C→A A309A (GCG→GCT fruA ← fructose‑specific PTS multiphosphoryl transfer protein FruA
RA 2,950,065 C→A A275E (GCG→GAG)  argA → N‑acetylglutamate synthase
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,810,859 G→T S98R (AGC→AGA mutM ← DNA‑formamidopyrimidine glycosylase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,878,724 C→T E466K (GAA→AAA)  gyrB ← DNA gyrase subunit B
RA 3,932,491 G→T W392C (TGG→TGT kup → K(+):H(+) symporter Kup
RA 4,070,417 G→T intergenic (‑16/+98) yihR ← / ← yihS putative aldose 1‑epimerase YihR/sulfoquinovose isomerase
RA 4,205,328 C→A D202Y (GAC→TAC)  purD ← phosphoribosylamine‑‑glycine ligase
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,344,953 C→G R202R (CGG→CGC yjdF ← conserved inner membrane protein YjdF

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102970 3104072 1103 38 [35] [35] 37 mutY mutY
* * ÷ NC_000913 3423771–3424234 3424533–3424238 5–763 36 [34] [34] 36 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)114 (0.960) 58/268 0.5 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =197 (1.610)234 (1.930) 90/274 0.0 70.4% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 1 (0.010)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)143 (1.170) 74/276 0.2 95.3% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 7 (0.060)coding (3907/4134 nt) rhsA rhs element protein RhsA