breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 312,327 A→T R72R (CGA→CGT ykgL → uncharacterized protein YkgL
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
JC JC 2,313,928 IS2 (–) +5 bp coding (441‑445/2673 nt) rcsD → RcsD phosphotransferase
RA 2,339,171 C→G A84P (GCG→CCG)  gyrA ← DNA gyrase subunit A
RA 2,906,299 G→T G197C (GGC→TGC)  ygcG → protein YgcG
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,392,446 Δ1 bp intergenic (+11/‑26) glyV → / → glyX tRNA‑Gly/tRNA‑Gly
RA 4,392,453 C→T intergenic (+18/‑19) glyV → / → glyX tRNA‑Gly/tRNA‑Gly
RA 4,392,456 A→G intergenic (+21/‑16) glyV → / → glyX tRNA‑Gly/tRNA‑Gly
RA 4,496,732 C→T intergenic (+243/‑18) leuX → / → intB tRNA‑Leu/KpLE2 phage‑like element; putative integrase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196275 1211431 15157 13 [11] [12] 15 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3423785–3424230 3424525–3424238 9–741 16 [10] [8] 14 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)66 (1.220) 44/268 0.2 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =72 (1.290)71 (1.280) 43/274 0.2 65.9% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 2 (0.040)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)46 (0.830) 37/276 0.4 92.0% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 4 (0.070)coding (3907/4134 nt) rhsA rhs element protein RhsA