breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
MC JC 1,299,499 Δ1,199 bp insH21 insH21
JC JC 1,638,416 IS1 (–) +9 bp coding (21‑29/189 nt) ynfQ ← Qin prophage; protein YnfQ
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,329,057 C→T V247V (GTG→GTA yfaQ ← tandem DUF2300 domain‑containing protein YfaQ
RA 2,950,734 A→G R576R (CGT→CGC recD ← exodeoxyribonuclease V subunit RecD
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
JC 3,495,041 (CCTGGCGCACG)1→2 coding (1031/2544 nt) nirB → nitrite reductase catalytic subunit NirB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,851,687 A→T D366E (GAT→GAA ilvB ← acetohydroxy acid synthase I subunit IlvB
RA 4,294,195 A→G intergenic (+56/‑286) nrfG → / → gltP putative formate‑dependent nitrite reductase complex subunit NrfG/glutamate/aspartate : H(+) symporter GltP
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196283 1211432 15150 14 [12] [13] 14 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3423766–3424233 3424525–3424239 7–760 14 [12] [9] 16 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3363001 =115 (2.050)76 (1.370) 46/274 0.1 56.9% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 1 (0.020)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)56 (1.000) 42/276 0.2 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 0 (0.000)coding (3907/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 4542682 =51 (0.910)14 (0.270) 11/258 2.9 22.8% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 47 (0.900)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 454269051 (0.910)18 (0.340) 17/258 1.8 27.6% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 47 (0.900)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit